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Plant Physiology 98:971-978 (1992)
© 1992 American Society of Plant Biologists

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Membranes and Bioenergetics

Identification and Characterization of Lipoxygenase Isoforms in Senescing Carnation Petals

Marie-Aude Rouet-Mayer, Jean-Marc Bureau and Christiane Laurière

Laboratoire de Physiologie des Organes Végétaux, Centre National de la Recherche Scientifique, 4ter route des Gardes, 92190 Meudon, France

A membrane-associated lipoxygenase and a soluble lipoxygenase have been identified in carnation (Dianthus caryophyllus L. cv Rêve) petals. Treatments of microsomal membranes by nonionic or zwitterionic detergents indicated that lipoxygenase is tightly bound to the membranes. By phase separation in Triton X-114, microsomal lipoxygenase can be identified in part as an integral membrane protein. Soluble lipoxygenase had an optimum pH range of 4.9 to 5.8, whereas microsomal lipoxygenase exhibited maximum activity at pH 6.1. Both soluble and membrane-associated lipoxygenases produced carbonyl compounds and hydroperoxides simultaneously, in the presence of oxygen. The membranous enzyme was fully inhibited by 0.1 millimolar n-propyl gallate, nordihydroguaiaretic acid, or salicylhydroxamic acid, but the effect of the three inhibitors on the soluble enzyme was much lower. The soluble lipoxygenase is polymorphic and three isoforms greatly differing by their isoelectric points were identified. Lipoxygenase activity in flowers was maximal at the beginning of withering, both in the microsomal and the soluble fractions. Substantial variations in the ratio of the two forms of lipoxygenase were noted at different sampling dates. Our results allowed us to formulate the hypothesis of a strong association of one soluble form with defined membrane constituents.




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P. Veronico, D. Giannino, M. T. Melillo, A. Leone, A. Reyes, M. W. Kennedy, and T. Bleve-Zacheo
A Novel Lipoxygenase in Pea Roots. Its Function in Wounding and Biotic Stress
Plant Physiology, July 1, 2006; 141(3): 1045 - 1055.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Society of Plant Biologists