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Plant Physiology 98:1336-1341 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Cell Wall Free Space of Cucumis Hypocotyls Contains NAD and a Blue Light-Regulated Peroxidase Activity 1

James R. Shinkle, Steven J. Swoap2, Patrice Simon3 and Russell L. Jones

Department of Plant Biology, University of California, Berkeley, California 94720, Department of Biology, Trinity University, San Antonio, Texas 78212

Solutions were obtained from the cell wall free space of red light-grown cucumber (Cucumis sativus L.) hypocotyl sections by a low-speed centrifugation technique. The centrifugate contained NAD and peroxidase but no detectable cytoplasmic contamination, as indicated by the absence of the activity of glucose-6-phosphate dehydrogenase from the cell wall solution. Peroxidase activity centrifuged from the cell wall of red light-grown cucumber hypocotyl section could be resolved into at least three cathodic isoforms and two anodic isoforms by isoelectric focusing. Treatment of red light-grown cucumber seedlings with a 10-minute pulse of high-intensity blue light increased the level of cell wall peroxidase by about 60% and caused a qualitative change in the anodic isoforms of this enzyme. The increase in peroxidase activity was detectable within 25 minutes after the start of the blue light pulse, was maximal at 35 minutes, and declined to control levels by 45 minutes of irradiation. The inhibitory effect of blue light on hypocotyl elongation was more rapid than the effect of blue light on total wall peroxidase activity, leading to the conclusion that growth and peroxidase activity are not causally related.

2 Present address: Department of Physiology, University of California, Irvine, CA 92715.

3 Present address: Laboratoire de Physiologie Vegetale, Universite de Genev, CH-1211 Geneve 4, Switzerland.

1 This work was supported by grants to R.L.J. from the National Science Foundation and the U.S. Department of Energy.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1992 by the American Society of Plant Biologists