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Plant Physiology 98:1437-1443 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Estimation of Mesophyll Conductance to CO2 Flux by Three Different Methods 1

Francesco Loreto, Peter C. Harley, Giorgio Di Marco and Thomas D. Sharkey

Istituto di Radiobiochimica ed Ecofisiologia Vegetali, (CNR-IREV), Area della Ricerca del CNR, 00016 Monterotondo Scalo, Roma, Italy, Systems Ecology Research Group, San Diego State University, San Diego, California 92182, Department of Botany, University of Wisconsin, Madison, Wisconsin 53706

The resistance to diffusion of CO2 from the intercellular airspaces within the leaf through the mesophyll to the sites of carboxylation during photosynthesis was measured using three different techniques. The three techniques include a method based on discrimination against the heavy stable isotope of carbon, 13C, and two modeling methods. The methods rely upon different assumptions, but the estimates of mesophyll conductance were similar with all three methods. The mesophyll conductance of leaves from a number of species was about 1.4 times the stomatal conductance for CO2 diffusion determined in unstressed plants at high light. The relatively low CO2 partial pressure inside chloroplasts of plants with a low mesophyll conductance did not lead to enhanced O2 sensitivity of photosynthesis because the low conductance caused a significant drop in the chloroplast CO2 partial pressure upon switching to low O2. We found no correlation between mesophyll conductance and the ratio of internal leaf area to leaf surface area and only a weak correlation between mesophyll conductance and the proportion of leaf volume occupied by air. Mesophyll conductance was independent of CO2 and O2 partial pressure during the measurement, indicating that a true physical parameter, independent of biochemical effects, was being measured. No evidence for CO2-accumulating mechanisms was found. Some plants, notably Citrus aurantium and Simmondsia chinensis, had very low conductances that limit the rate of photosynthesis these plants can attain at atmospheric CO2 level.

1 Research supported by Department of Energy grant FG02-87ER 13785 to T.D.S. and National Research Council of Italy, Special Project RAISA, Sub-project No. 2, Paper No. 253 to G.D. F.L. was supported by Consiglio Nazionale della Ricerche and North Atlantic Treaty Organization fellowships, and P.C.H. was supported by a grant from the U.S. Department of Energy CO2 Research Division No. DE-FG03-86ER60490 to J.F. Reynolds, Systems Ecology Research Group, San Diego State University.




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