Plant Physiology 98:1484-1493 (1992)
© 1992 American Society of Plant Biologists
Development and Growth Regulation
Role of Abscisic Acid in the Induction of Desiccation Tolerance in Developing Seeds of Arabidopsis thaliana
Cor Meurs1,
Amarjit S. Basra2,
Cees M. Karssen and
Leendert C. van Loon
Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
In contrast to wild-type seeds of Arabidopsis thaliana and to seeds deficient in (aba) or insensitive to (abi3) abscisic acid (ABA), maturing seeds of recombinant (aba,abi3) plants fail to desiccate, remain green, and lose viability upon drying. These double-mutant seeds acquire only low levels of the major storage proteins and are deficient in several low mol wt polypeptides, both soluble and bound, and some of which are heat stable. A major heat-stable glycoprotein of more than 100 kilodaltons behaves similarly; during seed development, it shows a decrease in size associated with the abi3 mutation. In seeds of the double mutant from 14 to 20 days after pollination, the low amounts of various maturation-specific proteins disappear and many higher mol wt proteins similar to those occurring during germination are induced, but no visible germination is apparent. It appears that in the aba,abi3 double mutant seed development is not completed and the program for seed germination is initiated prematurely in the absence of substances protective against dehydration. Seeds may be made desiccation tolerant by watering the plants with the ABA analog LAB 173711 or by imbibition of isolated immature seeds, 11 to 15 days after pollination, with ABA and sucrose. Whereas sucrose stimulates germination and may protect dehydration-sensitive structures from desiccation damage, ABA inhibits precocious germination and is required to complete the program for seed maturation and the associated development of desiccation tolerance.
1 Present address: Department of Virology, Agricultural University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
2 Present address: Department of Botany, Punjab Agricultural University, Ludhiana 141004, India.
This article has been cited by other articles:
|
|
|
|
 
M.-K. Choy, J. A. Sullivan, J. C. Theobald, W. J. Davies, and J. C. Gray
An Arabidopsis mutant able to green after extended dark periods shows decreased transcripts of seed protein genes and altered sensitivity to abscisic acid
J. Exp. Bot.,
October 17, 2008;
(2008)
ern227v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Xie, X. Li, B. J. Glover, S. Bai, G.-Y. Rao, J. Luo, and J. Yang
Duplication and Functional Diversification of HAP3 Genes Leading to the Origin of the Seed-Developmental Regulatory Gene, LEAFY COTYLEDON1 (LEC1), in Nonseed Plant Genomes
Mol. Biol. Evol.,
August 1, 2008;
25(8):
1581 - 1592.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Comella, F. Pontvianne, S. Lahmy, F. Vignols, N. Barbezier, A. DeBures, E. Jobet, E. Brugidou, M. Echeverria, and J. Saez-Vasquez
Characterization of a ribonuclease III-like protein required for cleavage of the pre-rRNA in the 3'ETS in Arabidopsis
Nucleic Acids Res.,
March 27, 2008;
36(4):
1163 - 1175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Boudet, J. Buitink, F. A. Hoekstra, H. Rogniaux, C. Larre, P. Satour, and O. Leprince
Comparative Analysis of the Heat Stable Proteome of Radicles of Medicago truncatula Seeds during Germination Identifies Late Embryogenesis Abundant Proteins Associated with Desiccation Tolerance
Plant Physiology,
April 1, 2006;
140(4):
1418 - 1436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gallardo, C. Job, S. P.C. Groot, M. Puype, H. Demol, J. Vandekerckhove, and D. Job
Proteomics of Arabidopsis Seed Germination. A Comparative Study of Wild-Type and Gibberellin-Deficient Seeds
Plant Physiology,
June 1, 2002;
129(2):
823 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. R. Finkelstein, S. S. L. Gampala, and C. D. Rock
Abscisic Acid Signaling in Seeds and Seedlings
PLANT CELL,
May 1, 2002;
14(90001):
S15 - 45.
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-J. Wang, C.-S. Loh, H.-H. Yeoh, and W. Q. Sun
Drying rate and dehydrin synthesis associated with abscisic acid-induced dehydration tolerance in Spathoglottis plicata orchidaceae protocorms
J. Exp. Bot.,
March 1, 2002;
53(368):
551 - 558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gallardo, C. Job, S. P.C. Groot, M. Puype, H. Demol, J. Vandekerckhove, and D. Job
Proteomic Analysis of Arabidopsis Seed Germination and Priming
Plant Physiology,
June 1, 2001;
126(2):
835 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Meinke
A Homoeotic Mutant of Arabidopsis thaliana with Leafy Cotyledons
Science,
December 4, 1992;
258(5088):
1647 - 1650.
[Abstract]
[PDF]
|
|
|
|
|
