Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 99:180-185 (1992)
© 1992 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Augur, C.
Right arrow Articles by Albersheim, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Augur, C.
Right arrow Articles by Albersheim, P.
Agricola
Right arrow Articles by Augur, C.
Right arrow Articles by Albersheim, P.
Development and Growth Regulation

Further Studies of the Ability of Xyloglucan Oligosaccharides to Inhibit Auxin-Stimulated Growth 1

Christopher Augur, Lu Yu2, Keiichiro Sakai, Tomoya Ogawa, Pierre Sinaÿ, Alan G. Darvill and Peter Albersheim

Complex Carbohydrate Research Center and the Department of Biochemistry, The University of Georgia, Athens, Georgia 30602, The Institute of Physical and Chemical Research, Wako-Shi, Saitama, Japan, École Normale Supérieure, Laboratoire de Chimie, 24 Rue Lhomond, Paris, France

The structural features required for xyloglucan oligosaccharides to inhibit 2,4-dichlorophenoxyacetic acid-stimulated elongation of pea stem segments have been investigated. A nonasaccharide (XG9) containing one fucosyl-galactosyl side chain and an undecasaccharide (XG11) containing two fucosyl-galactosyl side chains were purified from endo-{beta}-1,4-glucanase-treated xyloglucan, which had been isolated from soluble extracellular polysaccharides of suspension-cultured sycamore (Acerpseudoplatanus) cells and tested in the pea stem bioassay. A novel octasaccharide (XG8') was prepared by treatment of XG9 with a xyloglucan oligosaccharide-specific {alpha}-xylosidase from pea seedlings. XG8' was characterized and tested for its ability to inhibit auxin-induced growth. All three oligosaccharides, at a concentration of 0.1 microgram per milliliter, inhibited 2,4-dichlorophenoxyacetic acid-stimulated growth of pea stem segments. XG11 inhibited the growth to a greater extent than did XG9. Chemically synthesized nona- and pentasaccharides (XG9, XG5) inhibited 2,4-dichlorophenoxyacetic acid-stimulated elongation of pea stems to the same extent as the same oligosaccharides isolated from xyloglucan. A chemically synthesized structurally related heptasaccharide that lacked a fucosyl-galactosyl side chain did not, unlike the identical heptasaccharide isolated from xyloglucan, significantly inhibit 2,4-dichlorophenoxyacetic acid-stimulated growth.

2 Present address: Chengdu Institute of Biology, Chengdu, Sichuan, Peoples Republic of China.

1 This work was supported in part by U.S. Department of Energy grants DE-FG09-85ER13425 and DE-FG09-87ER13810 as part of the U.S. Department of Agriculture/Department of Energy/National Science Foundation Plant Science Centers program.




This article has been cited by other articles:


Home page
 J BiochemHome page
T. Ishimizu, C. Hashimoto, R. Takeda, K. Fujii, and S. Hase
A Novel {alpha}1,2-L-Fucosidase Acting on Xyloglucan Oligosaccharides is Associated with Endo- -Mannosidase
J. Biochem., December 1, 2007; 142(6): 721 - 729.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. Yaoi, T. Nakai, Y. Kameda, A. Hiyoshi, and Y. Mitsuishi
Cloning and Characterization of Two Xyloglucanases from Paenibacillus sp. Strain KM21
Appl. Envir. Microbiol., December 1, 2005; 71(12): 7670 - 7678.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yaoi and Y. Mitsuishi
Purification, Characterization, Cloning, and Expression of a Novel Xyloglucan-specific Glycosidase, Oligoxyloglucan Reducing End-specific Cellobiohydrolase
J. Biol. Chem., December 6, 2002; 277(50): 48276 - 48281.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Wu and D. J. Cosgrove
Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins
J. Exp. Bot., September 1, 2000; 51(350): 1543 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Vargas-Rechia, F. Reicher, M. Rita Sierakowski, A. Heyraud, H. Driguez, and Y. Liénart
Xyloglucan Octasaccharide XXLGol Derived from the Seeds of Hymenaea courbaril Acts as a Signaling Molecule
Plant Physiology, March 1, 1998; 116(3): 1013 - 1021.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. Augur, V. Stiefel, A. Darvill, P. Albersheim, and P. Puigdomenech
Molecular Cloning and Pattern of Expression of an alpha-L-Fucosidase Gene from Pea Seedlings
J. Biol. Chem., October 20, 1995; 270(42): 24839 - 24843.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W.-D. Reiter, C. C. S. Chapple, and C. R. Somerville
Altered Growth and Cell Walls in a Fucose-Deficient Mutant of Arabidopsis
Science, August 20, 1993; 261(5124): 1032 - 1035.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1992 by the American Society of Plant Biologists