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Plant Physiology 99:468-472 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture 1

A Flow Cytometric Study

Kwan-Hung Lo2 and K. Peter Pauls

Department of Crop Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

The influence of donor plant growth conditions on microspore embryogenesis in rapeseed (Brassica napus) was studied for plants grown at 23/18°C (16/8 hours) under continuous light, 23/18°C (16/8 hours) with a light/dark (16/8 hours) cycle, 15/12°C (16/8 hours) under continuous light and 15/12°C (16/8 hours) with a light/dark (16/8 hours) cycle. Significantly higher embryo yields were obtained from microspore cultures initiated from donor plants grown at 15/12°C instead of 23/18°C. Flow cytometric measurements of the microspores isolated from 2.5- to 5.0-millimeter buds showed that the microspores isolated from low-temperature-grown plants had significantly lower log 90-degree light scatter to forward angle light scatter and log 90-degree light scatter to time of flight ratios than those isolated from high-temperature-grown plants, suggesting that the former are more translucent than the latter. Thus, the effect of donor plant growth temperature on microspore embryogenesis may be mediated by a change in the physiology of the microspore cell, which results in the reduction of its cytoplasmic granularity and/or exine density.

2 Present address: Department of Horticultural Science, University of Saskatchewan, Saskatoon, Canada S7N 0W0.

1 Supported by the Ontario Ministry of Agriculture and Food.






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Copyright © 1992 by the American Society of Plant Biologists