This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by Miernyk, J. A. Articles by Dennis, D. T. Search for Related Content PubMed PubMed Citation Articles by Miernyk, J. A. Articles by Dennis, D. T. Agricola Articles by Miernyk, J. A. Articles by Dennis, D. T.

Metabolism and Enzymology

A Developmental Analysis of the Enolase Isozymes from Ricinus communis ¹

Seed Biosynthesis Research Unit, U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, Illinois 61604, Biology Department, Queen's University, Kingston Ontario, Canada K7L 3N6

Enolase activity was measured in clarified homogenates of various tissues during the life cycle of the castor oil plant (Ricinus communis L. cv Baker 296). The proportions of total activity due to the plastid and cytosolic isozymes were determined after separation by ion-exchange chromatography. The contribution of the plastid isozyme varied from more than 30% of the total at the midpoint of endosperm development to less than 1% in mature leaves and roots. During endosperm development, enolase activity increased to a peak coincident with the maximum rate of storage lipid accumulation, then decreased to nearly undetectable levels in the mature seed. Plastid enolase protein, measured using an enzyme-linked immunosorbent assay, increased in parallel with the increase in activity but decreased less rapidly and was still easily detectable in mature seeds.

This article has been cited by other articles:

				N. L. Houston, M. Hajduch, and J. J. Thelen Quantitative Proteomics of Seed Filling in Castor: Comparison with Soybean and Rapeseed Reveals Differences between Photosynthetic and Nonphotosynthetic Seed Metabolism Plant Physiology, October 1, 2009; 151(2): 857 - 868. [Abstract] [Full Text] [PDF]

				S. K. Lal, C. Lee, and M. M. Sachs Differential Regulation of Enolase during Anaerobiosis in Maize Plant Physiology, December 1, 1998; 118(4): 1285 - 1293. [Abstract] [Full Text]