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Plant Physiology 99:751-754 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Extraction and Partial Characterization of the Ethylene-Forming Enzyme from Apple Fruit 1

J. Carlos Fernández-Maculet and Shang Fa Yang

Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616

Ethylene-forming enzyme (EFE) was isolated from apple (Malus domestica Borkh. cv Golden Delicious) fruit tissue. The enzyme activity in the homogenate is associated with the pellet fraction and can be solubilized with Triton X-100 or polyvinylpolypyrrolidone. The solubilized enzyme system resembles the in vivo system in that it exhibits a low Km (17 micromolar) for its substrate 1-aminocyclopropane-1-carboxylic acid (ACC), is stereospecific toward 2-ethyl-ACC stereoisomers for 1-butene production, and is inhibited by cobalt ions and {alpha}-aminoisobutyric acid. Intact preclimacteric fruits treated with exogenous ethylene showed a marked increase in in vivo EFE activity and this increase was accompanied by a parallel increase in in vitro EFE activity. These results support the notion that the isolated EFE represents the authentic in vivo activity.

1 This work was supported by grant DCM-9004129 from the National Science Fundation. J.C.F-M. was a recipient of a research fellowship awarded by Ministerio de Educación y Ciencia, Spain.




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Copyright © 1992 by the American Society of Plant Biologists