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Plant Physiology 99:1201-1207 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Alteration of Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Activities by Site-Directed Mutagenesis

Jennie B. Shen1 and William L. Ogren

Department of Agronomy, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801-3838, Photosynthesis Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Urbana, Illinois 61801-3838

Site-directed mutagenesis was performed on the 1.6 and 1.9 kilobase spinach (Spinacea oleracea) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase cDNAs, encoding the 41 and 45 kilodalton (kD) isoforms of the enzyme, to create single amino acid changes in the putative ATP-binding site of Rubisco activase (Lys-107, Gln-109, and Ser-112) and in an unrelated cysteine residue (Cys-256). Replacement of Lys-107 with Met produced soluble protein with reduced Rubisco activase and ATPase activities in both isoforms. Substituting Ala or Arg for Lys-107 produced insoluble proteins. Rubisco activase activity increased in the 41-kD isoform when Gln-109 was changed to Glu, but activity in the 45-kD isoform was similar to the wild-type enzyme. ATPase activity in the Glu-109 mutations did not parallel the changes in Rubisco activase activity. Rather, a higher ratio of Rubisco activase to ATPase activity occurred in both isoforms. The mutation of Gln-109 to Lys inactivated Rubisco activase activity. Replacement of Ser-112 with Pro created an inactive protein, whereas attempts to replace Ser-112 with Thr were not successful. The mutation of Cys-256 to Ser in the 45-kD isoform reduced both Rubisco activase and ATPase activities. The results indicate that the two activities of Rubisco activase are not tightly coupled and that variations in photosynthetic efficiency may occur in vivo by replacing the wild-type enzyme with mutant enzymes.

1 Present address: Experimental Station, E. I. Du Pont de Nemours & Co., P.O. Box 80402, Wilmington, DE 19880-0402.




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Copyright © 1992 by the American Society of Plant Biologists