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Environmental and Stress Physiology

Association of 70-Kilodalton Heat-Shock Cognate Proteins with Acclimation to Cold ¹

Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611, Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida 32610, Department of Pathology and Laboratory Medicine, University of Florida, Gainesville, Florida 32610

Exposure of young spinach seedlings (Spinacia oleracea L. cv Bloomsdale) to 5°C leads to an increase in the synthesis of several 79-kilodalton proteins that are present in leaf tissue grown at 20°C. Protein sequence analyses and immunological cross-reactivity indicate that this group of proteins belongs to the 70-kilodalton heat-shock family. Steady-state transcript levels and protein synthesis are increased two- to threefold within 1 day, but immunoblot analyses suggest that the steady-state concentration of this protein group in leaf tissue only gradually accumulates at low temperature. It is proposed that the increased synthesis of several members of the 70-kilodalton heat-shock family could result from an influence of low temperature on protein folding and/or assembly processes.

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