This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Samuels, A. L. Articles by Glass, A. D. M. Search for Related Content PubMed PubMed Citation Articles by Samuels, A. L. Articles by Glass, A. D. M. Agricola Articles by Samuels, A. L. Articles by Glass, A. D. M.

Membranes and Bioenergetics

Immunofluorescent Localization of Plasma Membrane H⁺-ATPase in Barley Roots and Effects of K Nutrition ¹

Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada

The plasma membrane H⁺-ATPase (PM-H⁺-ATPase) of barley (Hordeum vulgare L. cv Klondike) roots was assayed by cross-reaction on western blots and cryosections with an antibody against the PM-H⁺-ATPase from corn roots. Under conditions of reduced K availability, which have previously been shown to increase K influx by greater than 25-fold, there were only minor changes detected in PM-H⁺-ATPase levels. Antibody labeling of cryosections showed the relative distribution of PM-H⁺-ATPase among cell types in root tips and mature roots. Epidermal cells, both protoderm and mature root epidermis, including root hairs, had high levels of antibody binding. In mature roots, the stelar tissue showing the highest antibody binding was the companion cells of the phloem, followed by pericycle, xylem parenchyma, and endodermis.

This article has been cited by other articles:

				H. Shen, J. Chen, Z. Wang, C. Yang, T. Sasaki, Y. Yamamoto, H. Matsumoto, and X. Yan Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation J. Exp. Bot., March 1, 2006; 57(6): 1353 - 1362. [Abstract] [Full Text] [PDF]

				T. E. Sondergaard, A. Schulz, and M. G. Palmgren Energization of Transport Processes in Plants. Roles of the Plasma Membrane H+-ATPase Plant Physiology, September 1, 2004; 136(1): 2475 - 2482. [Full Text] [PDF]

				F. Ma and C. A. Peterson Frequencies of plasmodesmata in Allium cepa L. roots: implications for solute transport pathways J. Exp. Bot., May 1, 2001; 52(358): 1051 - 1061. [Abstract] [Full Text] [PDF]

				A.G. Netting pH, abscisic acid and the integration of metabolism in plants under stressed and non-stressed conditions: cellular responses to stress and their implication for plant water relations J. Exp. Bot., February 2, 2000; 51(343): 147 - 158. [Abstract] [Full Text] [PDF]

				T. Bibikova, T Jacob, I Dahse, and S Gilroy Localized changes in apoplastic and cytoplasmic pH are associated with root hair development in Arabidopsis thaliana Development, January 8, 1998; 125(15): 2925 - 2934. [Abstract] [PDF]