Plant Physiol.
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Plant Physiology 99:1704-1707 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Morphoregulatory Role of Thidiazuron 1

Substitution of Auxin and Cytokinin Requirement for the Induction of Somatic Embryogenesis in Geranium Hypocotyl Cultures

Christena Visser2, Javed A. Qureshi, Ravinder Gill and Praveen K. Saxena

Department of Horticultural Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron). In less than 2 weeks, somatic embryos were observed in treatments containing levels of thidiazuron (TDZ) ranging from 0.2 to 1.0 micromolar. The use of N6-benzylaminopurine in combination with indole-3-acetic acid also evoked embryogenesis, but the efficiency of somatic embryo production was significantly lower than that obtained with TDZ. Hypocotyl culture for only 2 days on TDZ-supplemented medium before transfer to a basal medium was sufficient for inducing somatic embryogenesis. This distinction between the induction and expression of embryogenesis may provide an experimental system for studying the developmental biology of somatic embryogenesis. Substitution of the auxin-cytokinin requirement for the induction of somatic embryogenesis by TDZ suggests the possibility of a novel mode of its action by modulation of endogenous growth regulators.

2 Recipient of NSERC graduate fellowship.

1 This research was supported by operating grants from the Natural Sciences and Engineering Research Council of Canada and the University Research Incentive Funds to P.K.S.






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Copyright © 1992 by the American Society of Plant Biologists