Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 16, 2007; 10.1104/pp.106.094953

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
143/4/1615    most recent
pp.106.094953v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matheson, L. A
Right arrow Articles by Brandizzi, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matheson, L. A
Right arrow Articles by Brandizzi, F.
Agricola
Right arrow Articles by Matheson, L. A
Right arrow Articles by Brandizzi, F.

Received December 18, 2006
Accepted February 7, 2007

Multiple Roles of ARF1 in Plant Cells Include Spatially-Regulated Recruitment of Coatomer and Elements of the Golgi Matrix

Loren A Matheson , Sally L Hanton , Marika Rossi , Maita Latijnhouwers , Giovanni Stefano , Luciana Renna , and Federica Brandizzi *

Department of Biology, 112 Science Place, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada; Plant Pathology Program, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK; Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA

* Corresponding author; email: brandizz{at}msu.edu.

Recent evidence indicates that ARF1 carries out multiple roles in plant cells that may be independent from the established effector complex COPI. To investigate potential COPI-independent functions, we have followed the dynamics of ARF1 and a novel putative effector, the plant golgin GDAP1 in living plant cells. We present data that ascribe a new role to ARF1 in plant cell membrane traffic by showing that the GTPase functions to recruit GDAP1 to membranes. In addition, although ARF1 appears to be central to the recruitment of both COPI components and the golgin, we have established a different subcellular distribution of these ARF1 effectors. Live cell imaging demonstrates that GDAP1 and COPI are distributed on Golgi membranes. However, GDAP1 is also found on ARF1-labeled structures that lack coatomer, suggesting that the membrane environment, rather than ARF1 alone, influences the differential recruitment of ARF1 effectors. In support of this hypothesis, fluorescence recovery after photobleaching analyses demonstrated that GDAP1 and COPI have different kinetics on membranes during the cycle of activation and inactivation of ARF1. Therefore, our data support a model where modulation of the cellular functions of ARF1 in plant cells encompasses not only the intrinsic activities of the effectors, but also differential recruitment onto membranes that is spatially regulated.




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Osterrieder, E. Hummel, C. M. Carvalho, and C. Hawes
Golgi membrane dynamics after induction of a dominant-negative mutant Sar1 GTPase in tobacco
J. Exp. Bot., October 27, 2009; (2009) erp315v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Held, A. Boulaflous, and F. Brandizzi
Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes
Plant Physiology, August 1, 2008; 147(4): 1469 - 1481.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. Latijnhouwers, T. Gillespie, P. Boevink, V. Kriechbaumer, C. Hawes, and C. M. Carvalho
Localization and domain characterization of Arabidopsis golgin candidates
J. Exp. Bot., December 1, 2007; 58(15-16): 4373 - 4386.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2007 by the American Society of Plant Biologists