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Published on March 9, 2007; 10.1104/pp.107.095653

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Received January 8, 2007
Accepted February 15, 2007

Magnaporthe grisea Infection Triggers RNA Variation and Antisense Transcript Expression in Rice

Malali Gowda , R-C. Venu , Huameng Li , Chatchawan Jantasuriyarat , Songbiao Chen , Maria Bellizzi , Vishal Pampanwar , HyeRan Kim , Ralph A. Dean , Eric Stahlberg , Rod Wing , Cari Soderlund , and Guo-Liang Wang *

Department of Plant Pathology, The Ohio State University, Columbus OH 43210, USA; Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, AZ 85721, USA; Arizona Genomics Institute and Department of Plant Sciences, University of Arizona, AZ 85721; Fungal Genomics Laboratory, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA; Ohio Supercomputer Center, The Ohio State University, Columbus, OH 43212, USA; Rice Genomics Laboratory, Hunan Agricultural University, Changsha, Hunan 410128, China

* Corresponding author; email: wang.620{at}osu.edu.

Rice blast, caused by the fungal pathogen Magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. In this study, a comprehensive analysis of the rice transcriptome after M. grisea infection was conducted using RL-SAGE. A total of 83,382 distinct 21-bp RL-SAGE tags were identified from 627,262 individual tags isolated from the resistant (R), susceptible (S) and control (C) libraries. The sequence analysis revealed that the tags in the R and S libraries had a significant reduced matching rate to the rice genomic and expressed sequences in comparison to the C library. The high level of one-nucleotide mismatches of the R and S library tags was due to nucleotide conversions. The A to G and U to C nucleotide conversions were the most predominant types, which were induced in the M. grisea infected plants. RT-PCR analysis showed that the expression of the adenine deaminase and cytidine deaminase genes was highly induced after inoculation. In addition, many antisense transcripts were induced in the infected plants and the expression of four antisense transcripts was confirmed by strand-specific RT-PCR. These results demonstrate that there is a series of dynamic and complex transcript modifications and changes in the rice transcriptome at the early M. grisea infection stages.






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