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Published on May 8, 2008; 10.1104/pp.108.117523

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Received February 5, 2008
Accepted April 29, 2008

The AP2/ERF-domain transcription factor ORA59 integrates jasmonic acid and ethylene signals in plant defense

Martial Pre , Mirna Atallah , Antony Champion , Martin De Vos , Corne M.J. Pieterse , and Johan Memelink *

Institute of Biology Leiden, Clusius Laboratory, Leiden University, Leiden, The Netherlands; Section of Phytopathology, Department of Biology, Utrecht University, Utrecht, The Netherlands (MDV, CP)

* Corresponding author; email: j.memelink{at}biology.leidenuniv.nl.

Plant defense against pathogens depends on the action of several endogenously produced hormones, including jasmonic acid (JA) and ethylene. In certain defense responses, JA and ethylene signaling pathways synergize to activate a specific set of defense genes. Here we describe the role of the Arabidopsis thaliana AP2/ERF-domain transcription factor ORA59 in JA and ethylene signaling and in defense. JA- and ethylene-responsive expression of several defense genes, including the plant defensin gene PDF1.2, depended on ORA59. As a result, overexpression of ORA59 caused increased resistance against the fungus Botrytis cinerea, whereas ORA59-silenced plants were more susceptible. Several AP2/ERF-domain transcription factors have been suggested to be positive regulators of PDF1.2 gene expression based on overexpression in stably transformed plants. Using two different transient overexpression approaches we found that only ORA59 and ERF1 were able to activate PDF1.2 gene expression in contrast to the related proteins AtERF1 and AtERF2. Our results demonstrate that ORA59 is an essential integrator of the JA and ethylene signal transduction pathways, and thereby provide new insight in the nature of the molecular components involved in the crosstalk between these two hormones.






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