Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 25, 2008; 10.1104/pp.108.119511

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
147/2/802    most recent
pp.108.119511v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Rudd, J. J.
Right arrow Articles by Hammond-Kosack, K.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rudd, J. J.
Right arrow Articles by Hammond-Kosack, K.
Agricola
Right arrow Articles by Rudd, J. J.
Right arrow Articles by Hammond-Kosack, K.

Received March 20, 2008
Accepted April 18, 2008

The wheat MAP-kinases TaMPK3 and TaMPK6 are differentially regulated at multiple levels during compatible disease interactions with Mycosphaerella graminicola

Jason J. Rudd *, John Keon , and Kim Hammond-Kosack

Centre for Sustainable Pest and Disease Management, Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK

* Corresponding author; email: jason.rudd{at}bbsrc.ac.uk.

Many race- or isolate-specific disease resistance responses of plants towards pathogens (incompatible interactions) invoke hypersensitive response (HR)-like programmed cell death (PCD), and the co-ordinate activation of mitogen-activated protein kinases homologous to Arabidopsis AtMPK6 and AtMPK3 (or Tobacco SIPK and WIPK), respectively. Resistance of wheat leaves to the necrotrophic fungal pathogen Mycosphaerella graminicola can also operate at an isolate-cultivar specific level. We confirm here that resistance is achieved without any sign of HR-like PCD during the incompatible interaction. Instead PCD is strictly associated with the compatible interaction and is triggered during disease symptom expression. A strong transcriptional activation of TaMPK3, the wheat homologue of Arabidopsis AtMPK3, was observed immediately preceding PCD and symptom development in the compatible interaction. Generation and use of TaMPK3 and TaMPK6 -specific antibodies in western blots and in coupled immunoprecipitation-protein kinase assays demonstrated that the TaMPK3 protein also accumulated, and was subsequently post-translationally activated, during the compatible interaction in parallel to PCD. In contrast, no increase in expression, protein levels or post-translational activation of TaMPK6 was observed at any stage of either compatible or incompatible interactions. However, the protein levels of TaMPK6 became markedly reduced during the compatible interaction coincident with the onset of TaMPK3 protein accumulation. These data highlight the emerging similarity between the signalling pathways triggered in a host plant during successful infection by a necrotrophic fungal pathogen with the resistance responses normally effective against biotrophs.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2008 by the American Society of Plant Biologists