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Published on October 16, 2009; 10.1104/pp.109.147033

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Received September 3, 2009
Accepted October 10, 2009

The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis

Satoshi Kidokoro , Kyonoshin Maruyama , Kazuo Nakashima , Yoshiyuki Imura , Yoshihiro Narusaka , Zabta K. Shinwari , Yuriko Osakabe , Yasunari Fujita , Junya Mizoi , Kazuo Shinozaki , and Kazuko Yamaguchi-Shinozaki *

Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan; Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ibaraki 305-8686, Japan; and Plant Science Center, RIKEN Yokohama Institute, Kanagawa 230-0045, Japan

* Corresponding author; email: kazukoys{at}jircas.affrc.go.jp.

Transcription factors of the DREB1/CBF family specifically interact with a cis-acting dehydration-responsive element/C-repeat (DRE/CRT) involved in low-temperature stress–responsive gene expression in Arabidopsis. Expression of DREB1s is induced by low temperatures and is regulated by the circadian clock under unstressed conditions. Promoter sequences of DREB1s contain six conserved motifs, Boxes I to VI. We analyzed the promoter region of DREB1C using transgenic plants and found that Box V with the G-box sequence negatively regulates DREB1C expression under circadian control. The region around Box VI contains positive regulatory elements for low-temperature-induced expression of DREB1C. Using yeast one-hybrid screens, we isolated cDNA encoding the transcriptional factor PIF7, which specifically binds to the G-box of the DREB1C promoter. The PIF7 gene was expressed in rosette leaves, and the PIF7 protein was localized in the nuclei of the cells. Transactivation experiments using Arabidopsis protoplasts indicated that PIF7 functions as a transcriptional repressor for DREB1C expression and that its activity is regulated by PIF7-interacting factors TOC1 and PhyB, which are components of the circadian oscillator and the red light photoreceptor, respectively. Moreover, in the pif7 mutant, expression of DREB1B and DREB1C was not repressed under light conditions, indicating that PIF7 functions as a transcriptional repressor for the expression of DREB1B and DREB1C under circadian control. This negative regulation of DREB1 expression may be important for avoiding plant growth retardation by the accumulation of DREB1 proteins under unstressed conditions.






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