Plant Physiol. (1998) 116: 529-537

Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H⁺-ATPase¹
IV. Fusicoccin Induces the Association between the Plasma Membrane H⁺-ATPase and the Fusicoccin Receptor

Claudio Olivari^*, Cristina Meanti, Maria Ida De Michelis, and Franca Rasi-Caldogno²

Dipartimento di Biologia, Università di Milano, via G. Celoria 26, 20133 Milano, Italy (C.O., C.M., F.R.-C.); and Istituto di Botanica, Università di Genova, corso Dogali 1c, 16136 Genova, Italy (M.I.D.M.)

	ABSTRACT

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Different approaches were utilized to investigate the mechanism by which fusicoccin (FC) induces the activation of the H⁺-ATPase in plasma membrane (PM) isolated from radish (Raphanus sativus L.) seedlings treated in vivo with (FC-PM) or without (C-PM) FC. Treatment of FC-PM with different detergents indicated that PM H⁺-ATPase and the FC-FC-binding-protein (FCBP) complex were solubilized to a similar extent. Fractionation of solubilized FC-PM proteins by a linear sucrose-density gradient showed that the two proteins comigrated and that PM H⁺-ATPase retained the activated state induced by FC. Solubilized PM proteins were also fractionated by a fast-protein liquid chromatography anion-exchange column. Comparison between C-PM and FC-PM indicated that in vivo treatment of the seedlings with FC caused different elution profiles; PM H⁺-ATPase from FC-PM was only partially separated from the FC-FCBP complex and eluted at a higher NaCl concentration than did PM H⁺-ATPase from C-PM. Western analysis of fast-protein liquid chromatography fractions probed with an anti-N terminus PM H⁺-ATPase antiserum and with an anti-14-3-3 antiserum indicated an FC-induced association of FCBP with the PM H⁺-ATPase. Analysis of the activation state of PM H⁺-ATPase in fractions in which the enzyme was partially separated from FCBP suggested that the establishment of an association between the two proteins was necessary to maintain the FC-induced activation of the enzyme.

	INTRODUCTION

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The phytoxin FC is a powerful effector of the PM H⁺-ATPase and has been widely used as a tool with which to study the mechanism of physiological modulation of this crucial enzyme (Marrè, 1979; Marrè et al., 1993).

A high-affinity FCBP has been identified from different plant tissues (Aducci and Ballio, 1989; Weiler et al., 1990), and highly purified FCBP preparations have been obtained from several plant materials (de Boer et al., 1989; Oecking and Weiler, 1991; Aducci et al., 1993; Korthout et al., 1994). Work on isolated PM vesicles and on proteoliposomes reconstituted with partially purified PM H⁺-ATPase and FCBP has shown that FC, upon binding to PM-localized FCBP, causes strong activation of the PM H⁺-ATPase (for review, see Aducci et al., 1995). Stimulation of the PM H⁺-ATPase is variable and erratic when FC is added to isolated PM, whereas it becomes more dramatic when FC is fed in vivo. This activation determines a shift in the pH optimum of the enzyme toward more alkaline values and a decrease in the apparent K_m for the substrate Mg-ATP (Rasi-Caldogno and Pugliarello, 1985; Rasi-Caldogno et al., 1986, 1993; De Michelis et al., 1991; Johansson et al., 1993; Olivari et al., 1993; Lanfermeijer and Prins, 1994).

The PM H⁺-ATPase has a C-terminal autoinhibitory domain (Palmgren et al., 1990, 1991), and biochemical analysis of FC- and trypsin-treated PM (the latter treatment resulting in the loss of the C-terminal tail) has shown that FC-induced activation of PM H⁺-ATPase depends on a conformational modification of the enzyme, leading to the displacement of the C terminus (De Michelis et al., 1992; Rasi-Caldogno et al., 1993; Johansson et al., 1993; Lanfermeijer and Prins, 1994).

The FCBP is a member of the 14-3-3 protein family (Korthout and de Boer, 1994; Marra et al., 1994; Oecking et al., 1994), highly conserved and ubiquitously expressed proteins that bind to a variety of proteins involved in signal transduction and cell cycle regulation (Aitken et al., 1992; Morrison, 1994). We recently compared the maximum FC-binding capacity and the amount of H⁺-ATPase in PM isolated from Arabidopsis thaliana and radish (Raphanus sativus L.) seedlings, and found evidence to suggest a one-to-one stoichiometry between the FCBP and PM H⁺-ATPase (De Michelis et al., 1996b), indicating that there is no amplification step between the signal perceived by the FCBP and the activation of the PM H⁺-ATPase. This suggests that the FC-induced activation of the PM H⁺-ATPase depends on the molecular interaction of the FC-FCBP complex with the enzyme.

These data are consistent with the hypothesis that FC-induced activation of PM H⁺-ATPase depends on a direct interaction of the FC-FCBP complex with the enzyme, leading to the displacement of the C-terminal autoinhibitory domain.

Marra et al. (1996) showed that solubilized PM H⁺-ATPase from maize roots treated in vivo with FC and fractionated by anion-exchange HPLC eluted separately with respect to FCBP and retained its activated state after enzyme insertion into liposomes, thus suggesting a permanent modification of the PM H⁺-ATPase not dependent on a direct interaction of the FC-FCBP complex with the enzyme.

In this work we applied different approaches (solubilization with different detergents and Suc-density gradient and anion-exchange FPLC) to separate the PM H⁺-ATPase in the PM fraction purified from radish seedlings treated in vivo with or without FC from the FC-FCBP and to then analyze the activation state of the enzyme. The results obtained show that FC binding to FCBP induces an interaction between the PM H⁺-ATPase and the FC-FCBP complex and suggest that such an interaction is necessary to activate the PM H⁺-ATPase.

	MATERIALS AND METHODS

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Germination of Seeds and Isolation of PM

Solubilization of PM H⁺-ATPase and the FC-FCBP Complex

Separation of Solubilized PM Proteins by Linear Suc Gradient

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Anion-Exchange FPLC

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PM H⁺-ATPase Activity

Treatment with asolectin was performed by incubation of samples diluted 1:1 with a sonicated solution containing 15 mg mL¹ asolectin, 10 mm Mops-bis-Tris propane, pH 7.0, 20% (v/v) glycerol, 5 mm EDTA, and 9.375 mg mL¹ dodecyl--d-maltoside for 8 min at room temperature (keeping constant the ratio between asolectin and dodecyl--d-maltoside). The standard assay medium was then added. The concentration of asolectin used during the assay was 300 µg mL¹.

FC RIA

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SDS-PAGE

Western Analysis

Statistics

	RESULTS

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Solubilization of PM H⁺-ATPase and the FC-FCBP Complex

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To compare the efficiency of different detergents to solubilize the PM H⁺-ATPase and the FC-FCBP complex, we evaluated the PM H⁺-ATPase activity and the amount of FC-FCBP complex in the pellet obtained after solubilization of PM purified from FC-treated seedlings (FC-PM) in different conditions. Evaluation of the FC-FCBP complex in the SN was not possible because of interference of detergents with the FC RIA (data not shown). Table II shows PM H⁺-ATPase activity and the FC-FCBP complex measured in the insoluble fraction after solubilization of FC-PM with n-octyl--d-glucopyranoside, Chaps, and dodecyl--d-maltoside. In agreement with the data shown in Table I, the detergents solubilized the PM H⁺-ATPase to different extents. The same pattern was obtained when the concentration of the FC-FCBP complex was evaluated; in all of the detergents tested, the percentage of the two proteins remaining in the pellet after solubilization was similar.

In native PM vesicles, FC-induced activation of PM H⁺-ATPase is much stronger at pH values typical of the cytoplasm of a plant cell (pH 7.5) than at the relatively acidic pH optimum of the enzyme; therefore, the ratio between the activity measured at pH 6.4 and that measured at pH 7.5 (the pH ratio) is lower in FC-PM compared with that measured in C-PM (Rasi-Caldogno and Pugliarello, 1985; Rasi-Caldogno et al., 1986, 1993; Schulz et al., 1990; De Michelis et al., 1991; Olivari et al., 1993; Johansson et al., 1993). The pH ratio is therefore a useful parameter to monitor the activation state of the PM H⁺-ATPase. Table III shows the PM H⁺-ATPase activity measured at pH 6.4 and 7.5 and the corresponding pH ratios in native PM and the SN after solubilization of C-PM and FC-PM with different detergents. All detergents increased the pH ratio of solubilized PM H⁺-ATPase with respect to that measured in native PM. However, in all conditions tested the pH ratio in the SN fraction from FC-PM was one-half that from C-PM, indicating that the enzyme solubilized from FC-PM maintained its activated state.

In PM vesicles isolated from different plant materials, lysoPC activates the PM H⁺-ATPase (Palmgren et al., 1991; Rasi-Caldogno et al., 1993; Lanfermeijer and Prins, 1994; De Michelis et al., 1996a). The biochemical characteristics of such an activation are quantitatively and qualitatively similar to those induced by proteolytic treatment of the PM H⁺-ATPase (Palmgren et al., 1990, 1991; Johansson et al., 1993; Rasi-Caldogno et al., 1993). C-terminal deletion analysis of PM H⁺-ATPase expressed in yeast indicated that at least 38 C-terminal residues are necessary to determine lysoPC-induced activation of the PM H⁺-ATPase (Regenberg et al., 1995). Accordingly, in native PM, activation of PM H⁺-ATPase by FC and lysoPC are not additive, suggesting an at least partially common mechanism involving the displacement of the C-terminal inhibitory domain (Johansson et al., 1993; Rasi-Caldogno et al., 1993; De Michelis et al., 1996a).

We tested the effect of lysoPC on the PM H⁺-ATPase solubilized from C-PM and FC-PM. Figure 1 shows the PM H⁺-ATPase activity measured at pH 7.5 in the SN obtained after solubilization of C-PM and FC-PM with dodecyl--d-maltoside in the presence of different concentrations of lysoPC. The PM H⁺-ATPase activity solubilized from C-PM was stimulated by lysoPC in a concentration-dependent manner: stimulation increased up to 20 µg mL¹ (106%) and slightly decreased upon further increase of lysoPC concentration. When SN from FC-PM was analyzed, the PM H⁺-ATPase activity was only scarcely stimulated (27%) by 20 µg mL¹ lysoPC and was severely inhibited by higher concentrations of lysoPC, thus confirming that the H⁺-ATPase solubilized from FC-PM retains its activated state.

View larger version (46K): [in this window] [in a new window]   Figure 1. Effect of lysoPC on C-PM and FC-PM H+-ATPase activity solubilized with dodecyl--d-maltoside (1 mg protein mL1:1 mg detergent mL1). The assay was performed at pH 7.5 and lysoPC was added to the assay medium at 10 µg mL1 (light gray bar), 20 µg mL1 (gray bar), or 40 µg mL1 (black bar). PM H+-ATPase activity is expressed as a percentage of that measured in the absence of lysoPC (open bars): 29 nmol Pi min1 mg1 for C-PM and 67 nmol Pi min1 mg1 for FC-PM.

Fractionation of Solubilized PM Proteins by Suc-Density Gradient and Anion-Exchange FPLC

To discriminate between the two hypotheses we fractionated the SN from FC-PM obtained after solubilization with dodecyl--d-maltoside (3 mg proteins mL¹:3 mg detergent mL¹) by a linear Suc gradient (5-38% [w/w]). Figure 2 shows the distribution profile of the PM H⁺-ATPase activity and of the FC-FCBP complex: the two proteins comigrated, forming a sharp peak at fraction 13 (corresponding to 26% [w/w] Suc). The profile of the PM Ca²⁺-ATPase analyzed in the same gradient was clearly separated (data not shown). We also fractionated the SN from C-PM after solubilization in the same conditions and compared the pH ratios of the PM H⁺-ATPase activity in the peak gradient fractions from C-PM and FC-PM. Table IV shows the PM H⁺-ATPase activities at pH 6.4 and 7.5 and the pH ratios measured in the solubilized PM before fractionation and in three peak gradient fractions: although the pH ratio increased after fractionation, in the fractions from FC-PM this ratio was always much lower than that measured in the fractions from C-PM, indicating that after Suc-gradient fractionation the PM H⁺-ATPase from FC-PM maintained its activated state.

View larger version (14K): [in this window] [in a new window]   Figure 2. Distribution profiles of PM H+-ATPase activity () and the FC-FCBP complex () after solubilization of 6 mg of FC-PM proteins with dodecyl--d-maltoside (3 mg protein mL1:3 mg detergent mL1) and fractionation by a linear Suc-density gradient (5-38% [w/w]). PM H+-ATPase activity was measured at pH 6.4 in the presence of 10% glycerol (v/v). The FC-FCBP complex was assayed as FC RIA (see ``Materials and Methods''). PM H+-ATPase activity and amount of FC-FCBP are expressed as percentages of the peak fraction (822 nmol Pi min1 and 150 pmol bound FC, respectively).

To understand whether FC-induced activation of the PM H⁺-ATPase depends on an interaction with the FC-FCBP complex, it was necessary to find experimental conditions that would separate the two proteins. Marra et al. (1996) recently reported that, in PM from maize (Zea mays) roots incubated in vivo with FC, washed with Triton X-100 prior to solubilization with dodecyl--d-maltoside, and purified by DEAE-anion-exchange HPLC, the PM H⁺-ATPase and bound [³H]FC showed distinct elution profiles. We applied the same solubilization protocol to C-PM and FC-PM from radish seedlings. Table V shows the PM H⁺-ATPase activity measured in native PM, in the pellet obtained after washing PM with Triton X-100, and in the pellet and SN obtained after solubilization with dodecyl--d-maltoside. Treatment of the PM with Triton X-100 caused an approximate 30% decrease in the PM H⁺-ATPase activity, which was restored by the addition of asolectin. Solubilization of Triton-X-100-washed PM with dodecyl--d-maltoside determined an almost complete loss of the PM H⁺-ATPase activity, both in the pellet and in the soluble fractions, probably due to delipidation of the enzyme. The solubilized PM H⁺-ATPase activity of both C-PM and FC-PM was partially restored by the addition of asolectin.

View this table: [in this window] [in a new window]   Table V. Solubilization of PM H+-ATPase from Triton X-100-washed C-PM and FC-PM PM protein (1 mg) was treated with 0.36% (v/v) Triton X-100. Solubilization was performed with 10 mg mL1 dodecyl--d-maltoside in the presence of 2 mg protein mL1. PM H+-ATPase activity was assayed at pH 6.4 in the presence or absence of 300 µg mL1 asolectin.

Proteins from C-PM and FC-PM, solubilized as described in Table V, were fractionated by FPLC on a Mono-Q column. Figure 3 shows the elution profiles of the PM H⁺-ATPase activity from C-PM and FC-PM and the FC-FCBP complex from FC-PM. When solubilized C-PM was fractionated, the PM H⁺-ATPase eluted as a narrow peak at approximately 0.25 m NaCl (fractions 17-22), in agreement with results obtained with other plant materials (Johansson et al., 1994). Fractionation of solubilized FC-PM gave a different PM H⁺-ATPase elution profile. In fact, in this case the PM H⁺-ATPase activity eluted between 0.24 and 0.36 m NaCl (peaking at fractions 23-24, corresponding to 0.31 m NaCl), with a shoulder between 0.24 and 0.27 m NaCl (fractions 19-21) that partially overlapped the peak of C-PM H⁺-ATPase activity.

View larger version (22K): [in this window] [in a new window]   Figure 3. Elution profiles of PM H+-ATPase activity and the FC-FCBP complex after solubilization of 18 mg of C-PM and FC-PM proteins with dodecyl--d-maltoside (2 mg protein mL1:10 mg detergent mL1) and fractionation by FPLC Mono-Q anion-exchange column. , PM H+-ATPase activity from C-PM; , PM H+-ATPase from FC-PM; , FC-FCBP complex; dashed line, proteins; and continuous line, NaCl gradient. PM H+-ATPase activity, assayed in the presence of 300 µg mL1 asolectin, and the amount of FC-FCBP complex are expressed as a percentage of the peak fraction (313.2 nmol Pi min1 for C-PM, 156.6 nmol Pi min1 for FC-PM, and 414-pmol-bound FC, respectively). PM H+-ATPase activity was measured at pH 6.4. Proteins in the peak fractions were 126 µg for C-PM and 72 µg for FC-PM.

The FC-FCBP complex, measured by FC RIA in the fractions from FC-PM, showed a narrow peak at approximately 0.31 m NaCl, closely matching the major peak of the PM H⁺-ATPase activity. These results were confirmed by western analysis. As shown in Figure 4A, when the FPLC fractions from FC-PM were probed with an antiserum against the N-terminal domain of the PM H⁺-ATPase, the immunodecoration of the 100-kD polypeptide paralleled the elution profile of the PM H⁺-ATPase activity. The elution profile was asymmetric: the intensity of the bands increased from fractions 20 to 24, with a peak distributed between fractions 23 and 24, and then sharply decreased.

View larger version (57K): [in this window] [in a new window]   Figure 4. Immunoblotting of fractions 20 to 28, eluted from an FPLC anion-exchange Mono-Q column of solubilized FC-PM. A, Immunodecoration with anti-N-terminus PM H+-ATPase antibodies; B, immunodecoration with anti-14-3-3 antibodies.

It was recently demonstrated that the FCBP belongs to the family of 14-3-3 proteins (Aitken et al., 1992; Korthout and de Boer, 1994; Marra et al., 1994; Morrison, 1994; Oeking et al., 1994; de Boer, 1997). A 20-amino acid synthetic peptide, corresponding to an N-terminal sequence of the 14-3-3 present in purified FCBP from corn shoots, was used to produce polyclonal antibodies (Marra et al., 1994). We utilized this antiserum to probe fractions of the FPLC from FC-PM (Fig. 4B). The antiserum labeled a band of 30 kD, and to a much lesser extent a slightly smaller band, in fractions 20 to 28. The immunodecoration of the 30-kD band showed a sharp peak at fraction 24, matching the elution profile of the FC-FCBP complex measured by FC RIA (Fig. 3); the higher sensitivity of this approach revealed the presence of the FCBP in fractions in which it was not detectable by FC RIA (compare fractions 20, 21, and 27 in Figs. 3 and 4B).

Figure 5A shows the results of western analysis of FPLC fractions 11 to 24 from C-PM probed with the antiserum against the N-terminal domain of the PM H⁺-ATPase. The immunodecoration of the 100-kD polypeptide matched the elution profile of the PM H⁺-ATPase activity (Fig. 3), with maximal intensity of the bands in fractions 19 and 20. The availability of the anti-14-3-3 antiserum allowed us to analyze the distribution of the FCBP in FPLC fractions from C-PM as well. Figure 5B shows the results of western analysis of fractions 11 to 24 from C-PM probed with the anti-14-3-3 antiserum. The immunodecoration of the 30-kD band indicated that in this case the FCBP elution profile was completely different with respect to the PM H⁺-ATPase, which was monitored for hydrolytic activity (Fig. 3) and by western analysis (Fig. 5A), and eluted between 0.15 and 0.25 m NaCl (fractions 12-17). Moreover, the elution profile of the FCBP from C-PM was markedly different from that of the FCBP from FC-PM, which eluted at a much higher NaCl concentration (see western blot in Fig. 4B and FC RIA in Fig. 3).

View larger version (50K): [in this window] [in a new window]   Figure 5. Immunoblotting of fractions 11 to 24, eluted from FPLC anion-exchange Mono-Q column of solubilized C-PM. A, Immunodecoration with anti-N terminus PM H+-ATPase antibodies; B, immunodecoration with anti-14-3-3 antibodies.

As previously described, the FPLC profile obtained from FC-PM showed a shoulder of PM H⁺-ATPase between 0.24 and 0.27 m NaCl (fractions 19-21), where, as shown by western analysis (Fig. 4), PM H⁺-ATPase activity, and FC RIA (Fig. 3), the PM H⁺-ATPase and FCBP were partially separated. We compared the pH ratios of PM H⁺-ATPase activity in one fraction from C-PM (fraction 18) with two fractions from FC-PM showing similar PM H⁺-ATPase activity: fraction 21, in which the amount of FCBP was very low, and fraction 25, in which the two proteins co-eluted. Data in Table VI show that the pH ratios measured in fraction 18 from the C-PM profile and in fraction 25 from the FC-PM profile, which is within the peak of FC-FCBP, were similar to those measured in dodecyl--d-maltoside-solubilized PM H⁺-ATPase (Table III). Accordingly, the pH ratio measured in fraction 25 from FC-PM was about one-half that in fraction 18 from C-PM (3.4 versus 7.6), indicating that the PM H⁺-ATPase retained its activated state. In fraction 21 from FC-PM, in which the amount of FCBP was much lower than that of fraction 25, the pH ratio was 5.5, significantly higher than that measured in fraction 25, indicating that the PM H⁺-ATPase was partially inactivated in this fraction.

View this table: [in this window] [in a new window]   Table VI. pH ratio of PM H+-ATPase activity in FPLC fractions from C-PM and FC-PM Assays were performed on the specified fractions of the FPLC Mono-Q column in Figure 3 in the presence of 300 µg mL1 asolectin. The pH ratio is the ratio between the activity measured at pH 6.4 and that measured at pH 7.5.

	DISCUSSION

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In this paper we present evidence indicating that in vivo treatment of radish seedlings with FC causes an association between the PM H⁺-ATPase and the FC-FCBP complex necessary for the activation of the PM H⁺-ATPase.

The starting point of this work was our previous observation that in PM isolated from both A. thaliana and radish seedlings the stoichiometry between the FCBP and the PM H⁺-ATPase was close to one-to-one, suggesting that FC-induced activation of the PM H⁺-ATPase depends on a molecular interaction of the FC-FCBP complex with the enzyme (De Michelis et al., 1996b).

To further investigate this hypothesis, we utilized experimental conditions supposed to separate the two proteins and we analyzed the dependence of the FC-induced activation of PM H⁺-ATPase on its association with the FC-FCBP complex. Different detergents with diverse chemical characteristics solubilize the PM H⁺-ATPase and the FC-FCBP complex from FC-PM to similar extents (Table I-III). Moreover, the distribution profiles of PM H⁺-ATPase activity and of the FC-FCBP complex obtained by fractionation of solubilized FC-PM on a linear-density Suc gradient indicate that the two proteins comigrate (Fig. 2). These results are in agreement with the partial cosedimentation of the PM H⁺-ATPase activity with bound [³H]FC observed when microsomes from radish seedlings treated in vitro with FC were solubilized and fractionated on a Suc-density gradient (Cocucci and Marrè, 1991).

Marra et al. (1996) recently reported that in PM from maize roots incubated in vivo with FC, washed with Triton X-100 prior to solubilization with dodecyl--d-maltoside, and purified by DEAE-anion-exchange HPLC the PM H⁺-ATPase and bound [³H]FC showed distinct elution profiles. We applied the same experimental conditions to fractionate solubilized C-PM and FC-PM from radish seedlings by FPLC on a Mono-Q anion-exchange column but obtained only a partial separation of the two proteins (Fig. 3 and 4). In fact, both measurements of PM H⁺-ATPase activity and the FC-FCBP complex and western analysis performed with antibodies against the PM H⁺-ATPase and the 14-3-3 protein present in purified FCBP from different plant materials (Korthout and de Boer, 1994; Marra et al., 1994; Oecking et al., 1994) showed that the bulk of PM H⁺-ATPase and FCBP from FC-PM coelute between 0.24 and 0.36 m NaCl, with a peak at 0.31 m NaCl. Only a minor fraction of the PM H⁺-ATPase elutes at lower NaCl concentrations partially separated from the FCBP.

It is worth noting that, in agreement with previous observations (Johansson et al., 1994), the PM H⁺-ATPase from C-PM elutes at approximately 0.25 m NaCl, corresponding with the shoulder of PM H⁺-ATPase from FC-PM, where the enzyme is partially separated from the FCBP. Moreover, the 30-kD band of C-PM recognized by antibodies against the 14-3-3 elutes between 0.15 and 0.25 m NaCl, earlier than the PM H⁺-ATPase. Thus, FC treatment of the seedlings causes the establishment of a complex between the FCBP and the PM H⁺-ATPase. This complex, which might also involve other proteins, is stable to the solubilization procedure and elutes at a NaCl concentration higher than that necessary to elute the two proteins from C-PM. The discrepancy between our results and similar recently reported data (Piotrowsky et al., 1996) obtained with PM isolated from FC-treated leaves of Commelina communis and data obtained by Marra et al. (1996) from PM isolated from maize roots might reflect a weaker stability of the complex in graminaceous plants, but further investigations are necessary to understand this aspect.

As previously mentioned, FPLC fractionation of FC-PM allows partial separation of PM H⁺-ATPase from FCBP corresponding to fractions 19 to 21 (Figs. 3 and 4), where the relative amount of FC-FCBP is much lower than that of PM H⁺-ATPase. Analysis of the activation state of PM H⁺-ATPase (Table VI) in these fractions partially deprived of FCBP indicates that separation of the two proteins leads to inactivation of PM H⁺-ATPase, thus suggesting that the association between the FC-FCBP complex and PM H⁺-ATPase is necessary to the FC-induced activation of the enzyme.

Marra et al. (1996) showed that in FC-treated maize roots the PM H⁺-ATPase, once separated from the FCBP, retained its activated state. These results would be suggestive of an FC-induced covalent modification of the PM H⁺-ATPase; however, in those experiments western analysis to confirm the elution profile of the PM H⁺-ATPase activity was performed with an anti-C-terminus H⁺-ATPase antibody, which would not detect proteolyzed PM H⁺-ATPase activated by removal of the C-terminal domain (Palmgren et al., 1990, 1991; Rasi-Caldogno et al., 1993). On the other hand, Piotrowsky et al. (1996) recently showed that proteolytic removal of the C terminus leads to the release of FCBP from PM, suggesting that FCBP-specific 14-3-3 interacts directly with the PM H⁺-ATPase, presumably near or at the C terminus.

Data from several laboratories obtained with different plant materials indicate that FC binding to the FCBP stabilizes the coupling between FCBP and the PM H⁺-ATPase (Cocucci and Marrè, 1991; Korthout and de Boer, 1994; Oecking et al., 1994; De Michelis et al., 1996b; Piotrowsky et al., 1996). Evidence in this paper clearly confirms this association and indicates that it is necessary for activation of the PM H⁺-ATPase. The involvement of other proteins (e.g. protein kinases) in the formation of the complex cannot be ruled out. However, the lack of activation upon separation from the FC-FCBP complex suggests that activation does not depend on a covalent modification of the PM H⁺-ATPase.

Altogether, our results are in agreement with a recently proposed model for FC-induced activation of the PM H⁺-ATPase (de Boer, 1997). In this model the 14-3-3 forms a bridge between two domains of the PM H⁺-ATPase located in the C terminus and central loop (Aitken, 1996; Piotrowsky et al., 1996), analogous to the model for the Raf kinase-14-3-3 protein interaction (Aitken, 1996). In this conformation, the activity of the enzyme is low but stable. Activation of the PM H⁺-ATPase by different modulators such as FC, phosphatase 2A, or the phospho-Ser-259-Raf-1 peptide (Moorhead et al., 1996) would lead to the dissociation of the 14-3-3 from one domain, which by unfolding would determine the activation of the enzyme by dimerization or oligomerization, as reported for 14-3-3-induced oligomerization of Raf (Farrar et al., 1996). In light of the data available so far, the alternative proposed model, which explains the FC-induced activation of the PM H⁺-ATPase as due to the dissociation of 14-3-3 from the enzyme (Moorhead et al., 1996), is not supported by experimental evidence.

	FOOTNOTES

   Received July 21, 1997; accepted October 17, 1997.

	ABBREVIATIONS

Abbreviations: Chaps, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane-sulfonate. FC, fusicoccin. FCBP, FC-binding protein. FPLC, fast-protein liquid chromatography. [³H]FC, [³H]dihydrofusicoccin. lysoPC, lysophosphatidylcholine. PM, plasma membrane. RIA, radioimmunoassay. SN, supernatant.

	ACKNOWLEDGMENTS

The authors are grateful to Patrizia Aducci and Mauro Marra (Dipartimento di Biologia, Università di Roma Tor Vergata, Rome, Italy) for the generous gift of antiserum anti-FC and anti-14-3-3.

	LITERATURE  CITED

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