Plant Physiol. (1998) 116: 1451-1460

Polypeptides of the Maize Amyloplast Stroma¹
Stromal Localization of Starch-Biosynthetic Enzymes and Identification of an 81-Kilodalton Amyloplast Stromal Heat-Shock Cognate

Ying Yu, Helen He Mu, Chen Mu-Forster², and Bruce P. Wasserman^*

Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520

	ABSTRACT

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In the developing endosperm of monocotyledonous plants, starch granules are synthesized and deposited within the amyloplast. A soluble stromal fraction was isolated from amyloplasts of immature maize (Zea mays L.) endosperm and analyzed for enzyme activities and polypeptide content. Specific activities of starch synthase and starch-branching enzyme (SBE), but not the cytosolic marker alcohol dehydrogenase, were strongly enhanced in soluble amyloplast stromal fractions relative to soluble extracts obtained from homogenized kernels or endosperms. Immunoblot analysis demonstrated that starch synthase I, SBEIIb, and sugary1, the putative starch-debranching enzyme, were each highly enriched in the amyloplast stroma, providing direct evidence for the localization of starch-biosynthetic enzymes within this compartment. Analysis of maize mutants shows the deficiency of the 85-kD SBEIIb polypeptide in the stroma of amylose extender cultivars and that the dull mutant lacks a >220-kD stromal polypeptide. The stromal fraction is distinguished by differential enrichment of a characteristic group of previously undocumented polypeptides. N-terminal sequence analysis revealed that an abundant 81-kD stromal polypeptide is a member of the Hsp70 family of stress-related proteins. Moreover, the 81-kD stromal polypeptide is strongly recognized by antibodies specific for an Hsp70 of the chloroplast stroma. These findings are discussed in light of implications for the correct folding and assembly of soluble, partially soluble, and granule-bound starch-biosynthetic enzymes during import into the amyloplast.

	INTRODUCTION

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Starch is the major storage carbohydrate of higher plants, consisting of 25% amylose and 75% amylopectin in wild-type maize (Zea mays L.). The enzymes AGP, SS, SBE, and SU1 catalyze substrate formation, chain elongation, and branch-point insertion and trimming, respectively, as starch granules enlarge and develop. Various isoforms of these enzymes have been purified, cloned, and expressed in bacterial systems (Preiss, 1991; Martin and Smith, 1995; Wasserman et al., 1995), but the continuing emergence of new activities and isoforms in a range of species indicates that our understanding of the starch-biosynthetic pathway is far from complete. In addition, the relative contribution of specific enzyme isoforms to granule formation is regulated by factors such as subcellular localization and enzyme solubility within the starch matrix.

In nonphotosynthetic sink tissue, starch granules are contained within a specialized plastid known as the amyloplast (Lopes and Larkins, 1993; Nelson and Pan, 1995). Amyloplast-localized polypeptides are believed to be synthesized in the cytosol and are targeted to the amyloplast envelope by a transit peptide, which is proteolytically cleaved upon translocation through the amyloplast envelope into the stroma (Gavel and von Heijne, 1990; Li et al., 1992). For many years, it was believed that AGP was localized in the amyloplast stroma (Echeverria et al., 1985; Miller and Chourey, 1995). However, a recent study using maize amyloplasts isolated by gentle mechanical release showed that AGP activity is restricted largely to the cytosol (Denyer et al., 1996). A more detailed understanding of the compartmentalization of enzymes among the cytosol, the amyloplast stroma, and the starch granule is therefore vital for elucidating the mechanism of starch synthesis and deposition in the developing endosperm.

Morphologically, the amyloplast comprises three distinct components, the starch granule, the envelope, and the soluble compartment or stroma. Each of these components is characterized by a unique set of activities and polypeptides. In starch granules from common wild-type maize, the most abundant granule-associated polypeptide, the 60-kD GBSSI (waxy protein), is exclusively insoluble. In contrast, SSI and SBEIIb exist as both soluble and granule-associated forms (Mu-Forster et al., 1996). The amyloplast envelope from maize yields a complex set of integral membrane proteins. Of these, polypeptides in the size range of 39 to 44 kD have been implicated as isoforms of the putative adenylate-translocator BT1 (Cao et al., 1995; Sullivan and Kaneko, 1995). In contrast to the starch granule and the amyloplast envelope, the polypeptide composition of the amyloplast stroma has not been reported. Moreover, the hypothesis that the soluble SSs, SBEs, and ancillary polypeptides are localized within the stromal compartment of the amyloplast has never been directly tested or proven by probing stromal fractions with antibodies recognizing specific polypeptides.

The inability to analyze amyloplast stromal polypeptides was largely due to fragility of the amyloplast envelope. However, amyloplast-isolation methods based on direct, gentle mechanical release from isolated endosperms (Denyer et al., 1996) have now replaced protoplast-based procedures (Echeverria et al., 1985), thereby eliminating artifacts introduced by repeated centrifugations and use of cell wall-hydrolytic enzyme preparations of unknown purity. Although envelope fragility remains a major impediment to the use of uptake or metabolic studies requiring extensive handling or centrifugation steps (Echeverria et al., 1985, 1988), when physical handling is held to an absolute minimum, amyloplast preparations free of nonplastidial markers are readily obtained from immature (12-15 DAP) tissue.

The objective of this study was to identify and characterize polypeptides of the maize amyloplast stroma. Our working hypothesis is based on the prediction that the stroma contains a unique set of polypeptides, many of which are involved in starch granule formation. To achieve this objective, our strategy was to examine the enrichment of polypeptides specifically localized in the soluble stromal fraction. This identification is based on differential enrichment of polypeptides present in stromal fractions upon comparison with polypeptides of soluble extracts obtained from whole endosperm. Upon normalization for protein, polypeptides specifically localized in the amyloplast stroma are predicted to appear as differentially prominent bands in the soluble stromal fractions.

This approach yielded a well-defined group of polypeptides that are prominently displayed in the amyloplast stromal fractions. Through the combined use of highly specific antibodies and direct protein sequencing, we demonstrate that SSI, SBEIIb, and SU1 are each localized in the amyloplast stroma. Moreover, the stroma of maize contains a novel 81-kD member of the Hsp70 family of stress-related proteins. This 81-kD Hsc70 is constitutively expressed and is therefore classified and referred to as an Hsc. Hsp70s function as molecular chaperones in a wide range of organisms (Hendrick and Hartl, 1993; Hartl and Martin, 1995; Boston et al., 1996; Miernyk, 1997). The implications of molecular chaperones of the Hsp70 class within the maize amyloplast stroma are discussed in relation to protein import into the amyloplast and the folding of starch-biosynthetic enzymes.

	MATERIALS AND METHODS

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Preparation of Amyloplast Stromal Fractions

Soluble extracts from kernels were prepared as follows: 5 g of 15-DAP maize kernels was suspended in 5 mL of buffer A (10% glycerol, 10 mm EDTA, 1.25 mm DTT, and 50 mm Tris-HCl, pH 7.0), and mixtures were homogenized using a mortar and pestle. For immature 13-DAP maize, 7 g of kernels was combined with 34 mL of buffer A. Cellular debris were removed through two layers of Miracloth (Calbiochem). Filtrates were then centrifuged at 15,000g for 30 min. Soluble extracts were recovered, assayed for enzyme activities, and stored at 80°C.

Soluble extracts from isolated endosperms were prepared as follows: for 15-DAP maize, 5 g of endosperm was obtained by manual removal of embryos and pericarp and held on ice. Ten milliliters of buffer A was added and mixtures were homogenized using a mortar and pestle. For immature 13-DAP maize, 5 g of endosperm was combined with 5 mL of buffer A. Cellular debris were removed through two layers of Miracloth. Filtrates were then centrifuged at 15,000g for 20 min and soluble extracts were recovered.

Amyloplasts were isolated as previously described (Denyer et al., 1996), with BSA omitted from the isolation medium. Omission of BSA was necessary to conduct protein assays required for calculation of specific activities and for normalization of protein levels for SDS-PAGE. Ten grams of endosperm was obtained by manual dissection and placed in a tilted Petri dish containing amyloplast-isolation medium consisting of buffer B (0.8 m sorbitol, 1 mm EDTA, 1 mm KCl, 2 mm MgCl₂, 2 mm DTT, and 50 mm Hepes, pH 7.5) and incubated on ice for 30 min. A wide-bore pipette was used to slowly aspirate the cloudy liquid to a 30-mL round-bottom centrifuge tube. Endosperms were re-immersed in buffer B and sliced in half with a razor blade. The resultant extracts were transferred to centrifuge tubes using pipettes with the tips covered with Miracloth to filter out large particles. This procedure was repeated until the endosperms became soft. A yellow amyloplast-enriched pellet was recovered by centrifugation at 36g for 10 min. Amyloplasts were then resuspended in 1 mL of buffer A and stored at 80°C. Immediately before use, amyloplasts were thawed in the presence of 0.3% Triton X-100. A clear, soluble stromal fraction was recovered by centrifugation at 15,000g for 30 min.

A comparison of the SS and SBE activities of stromal fractions from amyloplasts released in the absence and presence of BSA demonstrated that omission of BSA did not alter levels of SS or SBE activities, their storage stability, or stromal polypeptide profiles in denaturing gels.

Starch Granule Isolation

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Enzyme Assays

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SBE activity was assayed by the phosphorylase a stimulation method (Hawker et al., 1974). Assay mixtures of 200 µL contained 50 mm [¹⁴C]Glc-1-P (1.3 µCi mmol¹), 1 mm AMP, 1.2 units of phosphorylase a (Sigma), 0.1 m sodium citrate, pH 7.0, and enzyme. Mixtures were incubated at 30°C for 30 min and reactions were terminated by heating at 100°C for 2 min. Radiolabeled reaction products were recovered by alcohol precipitation (2 mL of 75% [v/v] methanol and 1% [w/v] KCl), followed by centrifugation at 1600g for 3 min. Products were precipitated twice and radioactivity was determined by liquid-scintillation counting. A unit of activity is expressed as 1 µmol Glc incorporated into -d-glucan min¹.

AGP was assayed using the coupled-enzyme spectrophotometric assay in the presence of 10 mm 3-phosphoglyceric acid (Plaxton and Preiss, 1987). One unit of activity is defined as the amount of enzyme required to produce 1 µmol Glc-1-P min¹ at 37°C.

ADH assay mixtures contained 0.5 mL of 25 mm NAD, 0.25 mL of 2.0 m ethanol, and 0.75 mL of 0.1 m PPi buffer, pH 9.2, and reactions were initiated by the addition of 50 µL of sample. Formation of NADH was measured spectrophotometrically at 340 nm. Units are expressed as micromoles of NADH produced per minute (Vallee and Hoch, 1955).

Analytical Procedures

	RESULTS

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Detergent-Assisted Permeabilization of the Amyloplast Envelope

View larger version (21K): [in this window] [in a new window]   Figure 1. Effect of Triton X-100 on SS recovery and activity. A, SS levels of amyloplast stromal fractions. Amyloplasts were released from endosperm, as described in ``Materials and Methods'' and recovered by low-speed centrifugation. Stromal fractions were generated by suspension of amyloplasts in buffer A containing Triton X-100 at the levels indicated. Suspensions were mixed and centrifuged. Stromal fractions were recovered and assayed for SS activity. B, Effect of Triton X-100 on AGP (), SBE (), and SS () activities of a soluble endosperm extract. Endosperm extracts were prepared as described in ``Materials and Methods'' and aliquots were assayed at the indicated levels of Triton X-100.

These results show that total soluble endosperm activities of SS and AGP are neither activated nor inhibited by Triton X-100. SBE was activated 1.5-fold at 0.1% Triton X-100 but declined to 1.3-fold at 0.3 to 0.4%. This mild level of activation was not sufficient to explain the 10- to 20-fold elevation of SBE specific activity observed in Figure 2. Furthermore, during assays of stromal fractions, Triton X-100 levels were diluted 10-fold. Therefore, it must be concluded that the activity released from the amyloplasts (Fig. 1A) was due to Triton X-100-induced rupture of the amyloplast envelope rather than to detergent stimulation of enzyme activity.

View larger version (32K): [in this window] [in a new window]   Figure 2. Enrichment of SS and SBE in amyloplast stromal extracts. Specific activities of SS (A; nanomoles per minute per milligram), SBE (B; micromoles per minute per milligram), ADH, the cytosolic marker enzyme (C; micromoles per minute per milligram), and AGP assayed with 10 mm 3-phosphoglyceric acid (D; micromoles per minute per milligram). Solid and striped bars represent tissue harvested at 13 and 15 DAP, respectively. Values are representative of two independent isolations.

Cytosolic and Stromal Enzyme Activities

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This increase in specific activity could not be explained by the removal of an inhibitor because activity from the endosperm soluble fraction is linear with enzyme dilution and with time (Mu et al., 1994). Furthermore, if cytosolic activities were suppressed because of the presence of an inhibitor, the immunoblots showing dramatic enrichments of the SS, SBE, and SU1 polypeptides in the stromal fraction (Fig. 3) would not correlate with the activity data.

View larger version (55K): [in this window] [in a new window]   Figure 3. Immunoblots of endosperm and amyloplast stromal extracts probed with ADH, SSI, SBEIIb, and SU1 antibodies. SDS gels were run with amyloplast stromal fractions (Am), soluble extracts from whole endosperm (En), or SDS extracts corresponding to 2.5 mg of isolated starch granules (Gr). Immunoblots were probed with antibodies recognizing ADH (A), SSI (B), SBEIIb (C), and SU1 (D). The blots shown in A and D were visualized by electrochemiluminescence. The blots shown in B and C were visualized colorimetrically. Each lane contained 5 µg of protein.

No enrichment of the specific activity of the cytosolic marker ADH was observed (Fig. 2C). This finding is in accordance with the distribution of total enzyme activity described in the amyloplast AGP study (Denyer et al., 1996). Moreover, immunoblot analysis conducted using ADH antibodies (Good and Crosby, 1989) shows that the ADH polypeptide was barely detected in the stromal fraction (Fig. 3A). The partitioning of ADH toward the whole endosperm fraction supports the activity data (Fig. 2) and provides direct evidence that we are characterizing stroma and not a random set of endosperm polypeptides. Previous amyloplast studies have not used ADH antibodies to demonstrate the relative purity of stromal fractions.

Similarly, the specific activity of AGP in stromal fractions was not increased relative to whole endosperms (Fig. 2D), which is consistent with the finding that 95% of maize endosperm AGP activity is cytosolic (Denyer et al., 1996). Total activity recoveries in the stromal fraction relative to soluble endosperm extracts were 0.3% for ADH and 4 to 5% for SS and SBE, which agrees with yields previously reported (Denyer et al., 1996). The low yields of SS and SBE are attributed to the incomplete nature of amyloplast release from endosperms during the isolation procedure.

Immunoblot Analysis of Stromal Polypeptides

View larger version (95K): [in this window] [in a new window]   Figure 4. Immunoblots of endosperm and amyloplast stromal extracts probed with SH2 and BT2 antibodies. SDS gels were run with amyloplast stromal fractions (Am) or soluble extracts from whole endosperm (En). Immunoblots were probed with antibodies recognizing SH2, the AGP large subunit, or BT2, the AGP small subunit, as indicated. Each lane contained 5 µg of protein.

View larger version (89K): [in this window] [in a new window]   Figure 5. Protein composition of soluble extracts from whole endosperm and isolated amyloplasts. A, Coomassie blue-stained gel with 15 µg of protein per lane. B, Silver-stained gel with 10 µg of protein per lane. Shown are the soluble extracts from whole ground endosperm (En) and the amyloplast stromal fraction (Am). Marks indicate stromal proteins in descending order of apparent molecular mass: >220, 215, 180, 134, 112, 107, 85, 81, and 76 kD.

Consistent with the specific activity data (Fig. 2), immunoblots show that SSI (Fig. 3B) and SBEIIb (Fig. 3C) are highly concentrated in the amyloplast. The SU1 antibody recognized an 83-kD polypeptide, which is the deduced size of the SU1 gene product (James et al., 1995; Fig. 3D). Similar to SSI and SBEIIb, SU1 is highly concentrated in the amyloplast stroma. We also probed polypeptide extracts of starch granules isolated directly from the amyloplasts. SSI and SBEIIb were both present in SDS extracts of the granules; however, SU1 was detected in the soluble fraction only (Fig. 3D). Therefore, although SSI and SBEIIb both possess a propensity to associate with the starch granule (Mu-Forster et al., 1996), SU1 is soluble.

AGP subunits were probed using the SH2 and BT2 antibodies (Giroux and Hannah, 1994). These blots (Fig. 4) show that the large and small AGP subunits are distributed between the cytosol and the amyloplast stroma. However, in contrast to SSI, SBEIIb, and SU1, signal intensities for both SH2 and BT2 were weighted toward the soluble whole endosperm fraction. The faster-migrating BT2 signal at 50 kD in the stromal fraction is consistent with a uniquely compartmentalized stromal form (Denyer et al., 1996). The SH2 antibody also recognized multiple species. One immunoreactive species was detected in both samples, and a second species of lower molecular mass was detected in the soluble endosperm extract only. The occurrence of faster-migrating species of BT2 in the amyloplast stroma and SH2 in the soluble endosperm fraction can be interpreted as distinctly compartmentalized isoforms or could represent products of endogenous proteolytic activity (Plaxton and Preiss, 1987; Hannah et al., 1995).

Differentially Enriched Stromal Polypeptides

Polypeptides of >220, 215, 180, 134, 112, 107, 85, 81, and 76 kD were visibly enriched in the stromal fraction. Two of these, the 76- and 85-kD polypeptides, were positively identified as SSI and SBEIIb by using antibodies specific for these isoforms (Fig. 3). Consistent with our previous study (Mu et al., 1994), the 76-kD SSI was not readily discernible in the soluble extracts obtained from whole endosperm.

Of the newly established stromal polypeptides, the >220-, 215-, and 180-kD proteins stained poorly with Coomassie blue (Fig. 5A) but were readily apparent upon staining with silver (Fig. 5B). The 112-kD protein stained intensely with both Coomassie blue and silver. No significant differences in polypeptide profiles were observed between amyloplast fractions isolated from 13- or 15-DAP maize (data not shown). At least three polypeptides (96, 90, and 52 kD) were differentially enhanced in endosperm extracts, with a 52-kD polypeptide migrating at the expected position of the AGP small subunit.

To further establish the identity of amyloplast stromal polypeptides, several of the most abundant stromal polypeptides were excised from Coomassie blue-stained gels, electropurified, and subjected to N-terminal sequence analysis (Fig. 6). As a control, the stromal 85-kD polypeptide recognized by the SBEIIb antibody yielded the N-terminal sequence Ala-Ala-Ala-Arg-Lys-Val-Val-Met-Val-Pro. This corresponds to the predicted sequence of SBEIIb from W64A beginning at position 55 (Fisher et al., 1993). In W64A the N-terminal sequence of the mature protein was reported to begin with five Ala residues (Ala-Ala-Ala-Ala-Ala-Arg-Lys-Ala-Val-Met-Val-Pro) beginning at position 53 of the predicted sequence (Fisher et al., 1993). This difference between the two mature proteins may be varietal in nature.

View larger version (20K): [in this window] [in a new window]   Figure 6. N-terminal sequences of SBEIIb (85 kD; A) and the stromal Hsc70 (81 kD; B). Polypeptides were excised from SDS gels and electropurified as previously described (Mu-Forster et al., 1996). The dash at position 16 indicates an uncertain residue tentatively identified as an Arg residue. Published sequences were as follows: SBEIIb beginning at residue 53 of the predicted sequence (Fisher et al., 1993); chloroplast Hsp70 from Pisum sativum (Marshall and Keegstra, 1992); chloroplast Hsp70 from Curcurbita sp. (Tsugeki and Nishimura, 1993); chromoplast Hsp70 from Narcissus pseudonarcissus (Bonk et al., 1996); mitochondrial SSC1, an Hsp70 analog from Saccharomyces cerevisiae (Craig et al., 1989); dnaK, an Hsp70 analog from E. coli (Bardwell and Craig, 1984); and cytosolic Hsp70 from Z. mays endosperm (Rochester et al., 1986).

A Maize Amyloplast Stromal Hsc70

We then tested the identity of the 81-kD stromal polypeptide using antibodies generated against an Hsp70 from the chloroplast stroma of spinach (Wang et al., 1993; Fig. 7). As with the previous experiments, normalized soluble extracts from whole endosperm and amyloplasts were compared. Controls consisted of an Hsp70 preparation from E. gracilis (Amir-Shapira et al., 1990) and the purified stromal 81-kD polypeptide. The blot shows that the antibodies strongly recognized the 81-kD stromal polypeptide (Fig. 7). Consistent with SSI and SBEIIb, this polypeptide is enriched within the stromal fraction. The antibodies also detected an immunoreactive species at 70 kD, which migrates at the expected mass of a known endosperm cytosolic form of Hsp70 from maize endosperm (Rochester et al., 1986). Antibodies to BiP, the Hsp localized in the lumen of the ER (Fontes et al., 1991), did not recognize the stromal 81-kD Hsc70 polypeptide (not shown).

View larger version (36K): [in this window] [in a new window]   Figure 7. Immunoblots of endosperm and amyloplast stromal extracts probed with chloroplast Hsp70 antibody. Lane 1, Hsp70 preparation containing two closely migrating Hsp70 proteins purified from E. gracilis (Amir-Shapira et al., 1990; 0.1 µg); lane 2, soluble extract from whole endosperm (En; 30 µg); lane 3, amyloplast stromal fraction (Am; 30 µg); and lane 4, purified maize stromal 81-kD polypeptide (0.1 µg).

From these independent lines of evidence, it may be concluded that the 81-kD polypeptide is a stromal member of the Hsp70 family. Members of the Hsp70 family have been found in the stroma of several other types of plastids, such as the chloroplast (Marshall and Keegstra, 1992; Wang et al., 1993) and chromoplast (Bonk et al., 1996). This work shows that maize endosperm contains at least two distinct members of the Hsp70 family of stress-related proteins.

Analysis of Stroma from Mutant Cultivars

View larger version (68K): [in this window] [in a new window]   Figure 8. Polypeptide profiles of amyloplast stroma of amylose extender (ae) and dull (du) mutants. Stroma are from an Exs86 parental line (lane 1), an isogenic dull mutant (lane 2), and an amylose extender mutant (lane 3). Each lane contained 30 µg of protein.

Granule-Associated Proteins Are Not Selectively Solubilized by 0.3% Triton X-100

View larger version (50K): [in this window] [in a new window]   Figure 9. Effect of Triton X-100 on the extraction of soluble polypeptides from suspensions of whole endosperms and isolated amyloplasts. Lanes 1 and 2, Soluble fraction from whole endosperm; lanes 3 and 4, soluble fraction from isolated amyloplasts. Soluble extracts were prepared in buffer A in the presence (+) or absence () of 0.3% Triton X-100. Each lane contained 25 µg of protein.

	DISCUSSION

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The amyloplast is one of the most vital and yet least- understood organelles in starch-bearing crops. Therefore, detailed knowledge of the proteins that constitute the amyloplast envelope, the starch granule, and the stroma is essential to gain a complete understanding of starch deposition in developing grains. This study demonstrates that the amyloplast stroma provides a concentrated source of starch-biosynthetic enzymes and ancillary proteins, establishing a means to investigate the structure and function of individual stromal polypeptides. The utility of this approach was validated by positive identification of an amyloplast-localized Hsc70 of 81 kD.

If one assumes that each amyloplast is a 6-µm-diameter spherical particle and that the amyloplast stroma accounts for 10% of plastid volume, then soluble stromal contents are diluted by more than 100-fold when intact whole endosperm is subjected to homogenization. Because of this dilution effect, stromal proteins are greatly obscured by cytosolic polypeptides when soluble endosperm extracts are separated by SDS-PAGE. However, differential electrophoretic analysis of soluble extracts from the amyloplast stroma and whole endosperm provided a facile means to distinguish between stromal and cytosolic polypeptides. When soluble fractions from amyloplasts and endosperms were normalized for protein content, differential enrichment of SSI and SBEIIb was clearly evident (Fig. 3). Moreover, enrichment of these polypeptides on immunoblots was correlated with the significant elevation of SS- and SBE-specific activities (Fig. 2). In accordance, our results demonstrate that SU1 (James et al., 1995) is compartmentalized within the stroma. To further examine stromal fractions, two starch mutants were compared with wild-type cultivars. Stromal fractions from the amylose extender mutant were deficient in SBEIIb, whereas the dull mutant was lacking in a polypeptide of >220 kD. This size range is generally consistent with the estimated mass of SSII, which was estimated at 180 kD by gel-filtration chromatography (Mu et al., 1994).

Most significantly, this approach provided a means to identify novel stromal polypeptides. As a case in point, sequence analysis of an abundant 81-kD polypeptide provided evidence indicating that this polypeptide is an amyloplastic member of the Hsp70 family of stress-related proteins (Fig. 6). Further proof was obtained by showing that Hsc70 was strongly recognized by antibodies generated against a 75-kD chloroplast stromal Hsp70 (Fig. 7; Wang et al., 1993). In addition, the banding intensity of stromal Hsc70 paralleled that of SSI and SBEIIb (Fig. 7), as would be expected for a protein localized in the stroma. The antibodies also recognized the maize cytosolic Hsp70, which migrates at 70 kD, albeit with lesser intensity. Consistent with its localization within the cytosol, the 70-kD cytosolic Hsp70 was not differentially elevated in soluble stromal fractions.

Identification of stromal Hsc70s is significant because little is known about the way in which nuclear-encoded polypeptides are folded as they are translocated through the amyloplast envelope into the stroma. The existence of Hsp70 proteins in other plant plastids such as chloroplasts and chromoplasts is well established (Marshall and Keegstra, 1992; Bonk et al., 1996); however, to our knowledge, until now amyloplastic Hsps had not been identified. The N-terminal sequence of the maize stromal 81-kD Hsc70 bears more similarity to the plastidial and prokaryotic Hsp70s than to the N terminus of the 70-kD maize cytosolic Hsp70 (Fig. 6). The 81-kD maize stromal Hsc70 appears to be constitutively expressed, which would classify this protein as an Hsc (Miernyk, 1997). The finding of stromal Hsp70s would help to explain the process of polypeptide translocation across the amyloplast envelope and protein folding within the stroma, as illustrated in the working model (Fig. 10).

View larger version (37K): [in this window] [in a new window]   Figure 10. Working model. A proposed mechanism for the import and translocation of polypeptides into the amyloplast stromal compartment illustrating a possible mode of action of the 81-kD Hsc70. This model proposes that chaperones of the Hsp70 class facilitate the import and folding of enzymes that are fully (I) or partially (II) soluble within the stroma. A proposed function of the chaperones is to stabilize the partially soluble mature proteins, delaying their insolubilization within the starch matrix. A separate route for the import and deposition of fully insoluble (e.g. GBSSI) amyloplast proteins (III) is shown.

The amyloplast stroma is a tightly confined environment exhibiting high levels of metabolic activity. As stromal polypeptides cross the amyloplast envelope, the transit peptides are proteolytically cleaved, and each polypeptide must correctly fold to achieve its active conformation. A molecular chaperone could provide a vital function during this process by forming transient complexes with hydrophobic polypeptide domains to prevent aggregation and incorrect folding (Hendrick and Hartl, 1993; Hartl and Martin, 1995; Boston et al., 1996; Miernyk, 1997). Various mechanisms describing possible physical associations between molecular chaperones and translocated polypeptides have been proposed (Cohen et al., 1995; Glick, 1995; Boston et al., 1996).

Moreover, SSs and SBEs have the tendency to become entrapped within the starch matrix (Denyer et al., 1993; Rahman et al., 1995; Mu-Forster et al., 1996). In maize endosperm, only 10 to 20% of SSI is soluble, the remainder is intrinsically bound to the granule (Mu-Forster et al., 1996). In addition, activity gels of endosperm extracts reveal numerous species of SS (Schiefer et al., 1973). The existence of molecular chaperones within the stroma could help to explain these observations. We propose that stromal Hsc70 forms transient complexes with SSI and other stromal enzymes. A complex would serve dual purposes. One would be to provide correct folding. A second and equally important function would be to delay the entrapment of starch-synthetic enzymes within the starch matrix, extending the functional lifetime of the soluble enzymes.

The working model (Fig. 10) illustrates possible modes of import of proteins into the amyloplast. One class of proteins, which includes SU1 and the prominent 112-kD stromal polypeptide, remain fully soluble following import. A second class consists of SSI and SBEIIb, which are both soluble and granule associated. Stromal Hsc70 or other chaperones may maintain enzyme solubility before entrapment within the starch matrix. A third type of amyloplastic protein, typified by GBSSI, is completely bound to the starch granule. Our model proposes that GBSSI is transported across the envelope as a soluble polypeptide. Chaperones would enable GBSSI to function catalytically before its rapid entrapment within the starch matrix. It remains to be established whether the different classes of polypeptides are served by a single chaperone or by multiple forms.

This study also addressed the subcellular localization of AGP. Some studies have concluded that AGP is largely stromal (Echeverria et al., 1985; Miller and Chourey, 1995), but the fact that the in vitro-translated SH2 and BT2 subunits were the same size as the authentic endosperm subunits (Giroux and Hannah, 1994) has raised uncertainties concerning the true localization of AGP in maize endosperm tissue. Accordingly, one study demonstrated that up to 95% of AGP activity in maize cv UE95 was cytosolic (Denyer et al., 1996). Consistent with this notion, our results show no appreciable enhancement of AGP-specific activity in the stroma (Fig. 2). However, immunoblotting revealed significant levels of stromal SH2 and BT2 subunits at both 13 and 15 DAP (Fig. 4). Of the starch-synthetic enzymes located in the amyloplast stroma, AGP is the only known multimeric complex. The low stromal activity of AGP, therefore, may reflect an inability of the individual subunits to properly fold and assemble into a functionally active tetrameric complex following their import into the stroma. In contrast to SSI and SBEIIb, AGP is completely soluble and does not become embedded within the starch granule (Mu-Forster et al., 1996).

In summary, direct localization of SS, SBE, and SU1 as stromal enzymes supports earlier contentions (Echeverria et al., 1988) that the amyloplast contains the entire complement of proteins required for chain elongation, branching, and processing of starch polymers. Moreover, this organelle contains an abundant member of the Hsp70 family and additional polypeptides of unknown function (e.g. >220, 215, 180, 134, 112, and 107 kD) that may influence granule composition and morphology (Lopes and Larkins, 1993; Nelson and Pan, 1995). Knowledge of stromal polypeptide composition provides a potential basis for establishing the identity of multiple SS isoforms (Pollock and Preiss, 1980; Macdonald and Preiss, 1985; Mu et al., 1994) and for investigating the process by which starch-biosynthetic enzymes and ancillary proteins assume their active conformations following import across the amyloplast envelope.

	FOOTNOTES

   Received October 9, 1997; accepted December 3, 1997.

	ABBREVIATIONS

Abbreviations: ADH, alcohol dehydrogenase. AGP, ADP-Glc pyrophosphorylase. BT, brittle mutant. DAP, days after pollination. GBSSI, granule-bound starch synthase I. Hsc, heat-shock cognate. Hsp, heat-shock protein. SBE, starch-branching enzyme. SH, shrunken mutant. SS, starch synthase. SU1, sugary1 mutant, the starch-debranching enzyme.

	ACKNOWLEDGMENTS

Maize and initial amyloplast isolates were provided by Francie Dunlap, Ed Wilhelm, and Peter Keeling (ExSeed Genetics). We thank Rebecca S. Boston, Maureen Clancy, Alan Good, L. Curtis Hannah, Martha James, Thomas Leustek, Jan S. Miernyk, and Afroza Rahman for generously providing antibodies; Bing-Yuan Chen (Plant Science Department, Rutgers University) for assistance with AGP assays; and George M. Carman for helpful advice. Dave Lear and Joe Florentine assisted with greenhouse work.

	LITERATURE  CITED

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