Plant Physiol. (1998) 116: 1607
CORRECTION
Vol. 116: 687-693, 1998
Seo, M., Akaba, S., Oritani, T., Delarue, M., Bellini, C.,
Caboche, M., and Koshiba, T. Higher Activity of an Aldehyde Oxidase in
the Auxin-Overproducing superroot1 Mutant of
Arabidopsis thaliana.
The editorial office regrets that the abstract carried a misprint that
was made during the production process. The corrected sentence is
printed below followed by the entire abstract.
The activity was about 5 times higher in the extract of the
sur1 seedlings, indicating that AO1 also has a substrate
preference for indole-3-acetaldehyde.
Aldehyde oxidase (AO; EC
1.2.3.1) activity was measured in seedlings of wild type or an
auxin-overproducing mutant, superroot1 (sur1), of Arabidopsis thaliana. Activity
staining for AO after native polyacrylamide gel electrophoresis
separation of seedling extracts revealed that there were three major
bands with AO activity (AO1-3) in wild-type and mutant seedlings. One
of them (AO1) had a higher substrate preference for indole-3-aldehyde.
This AO activity was significantly higher in sur1 mutant
seedlings than in the wild type. The difference in activity was most
apparent 7 d after germination, the same time required for the
appearance of the remarkable sur1 phenotype, which
includes epinastic cotyledons, elongated hypocotyls, and enhanced root
development. Higher activity was observed in the root and hypocotyl
region of the mutant seedlings. We also assayed the
indole-3-acetaldehyde oxidase activity in extracts by high-performance
liquid chromatography detection of indole-3-acetic acid (IAA). The
activity was about 5 times higher in the extract of the
sur1 seedlings, indicating that AO1 also has a substrate
preference for indole-3-acetaldehyde. Treatment of the wild-type
seedlings with picloram or IAA caused no significant increase in AO1
activity. This result suggested that the higher activity of AO1 in
sur1 mutant seedlings was not induced by IAA accumulation and, thus, strongly supports the possible role of AO1 in
IAA biosynthesis in Arabidopsis seedlings.