Figure 1. Comparison of S. lepidophylla SlTPS1 with other TPS protein sequences. A, Alignment of an internal region of plant TPS amino acid sequences with bacteria, yeast, and animal sequences. Gaps that were introduced to optimize the alignment are indicated by dashes; identical residues are shaded. B, Dendrogram of TPS sequences. Amino acid sequences of TPS from (top to bottom) M. thermoautotrophicum (MtTPS1; accession no. AE000931); E. coli (EcOtsA; accession no. X69160); D. melanogaster (DmTPS1; accession no. AC004373); A. thaliana (AtTPS1; accession no. Y08568); S. lepidophylla (SlTPS1; accession no. U96736); S. cerevisiae (ScTPS1; accession no. X68214); and A. thaliana (AtTPS4; accession no. Z97344). DmTPS1 and AtTPS4 expressed sequence tags were found in databases from the respective systematic genome-sequencing programs. C, Diagram of TPS and TPP protein sequences. A comparison of protein size and structure is shown based on sequence-alignment data presented in A and compared with E. coli (EcotsB) and S. cerevisiae (ScTPS2) sequences. Protein size is shown in amino acid (aa) length. D, The 5 leader sequence of the SlTPS1 gene is shown. Three ATG triplets are present (boldface), but only the one close to the 3 end is in frame with the open reading frame sequence; thus, it is considered to be the putative initiation codon. The NcoI restriction site is underlined.

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