Stop-and-Go Movements of Plant Golgi Stacks are Mediated by the Acto-Myosin System

Andreas Nebenführ, Larry Gallagher, Terri G. Dunahay, Jennifer A. Frohlick, Anna M. Mazurkiewicz, Janet B. Meehl, L. Andrew Staehelin

manuscript in press in Plant Physiology (December 1999).

These video sequences demonstrate the movement of GFP-labeled Golgi stacks in tobacco BY-2 suspension culture cells. Specifically, these videos formed the basis for some of the figures in our paper analyzing the molecular basis for the Golgi stack movement.

All video sequences are shown at 4 frames per second and are speeded up by a factor of two.

Fig. 4 a: Movement in cortical cytoplasm [video] (2.2 MB)

Fig. 4 b: Movement in transvacuolar strands [video] (1.7 MB)

Fig. 4 c: Movement is stopped by cytochalasin D treatment [video] (1.7 MB)

Fig. 4 d: Movement appears unaffected by nocodazole treatment [video] (2.6 MB)

Fig. 2 c: Some stacks appear as rings [video] (1 MB; size bar = 5 µm)

Fig. 8: Shift of active region in cortical cytoplasm:
Short video sequences (10 s duration) were recorded at 10 min intervals [0 min, 10 min, 20 min], Golgi stacks were detected automatically using a peak-finding algorithm. Positions where stacks were detected in most frames (slowly moving and wiggling stacks) are coded in blue and green, positions where stacks were detected only rarely (fast moving stacks) are coded in yellow and red [0 min, 10 min, 20 min].

For further information you can contact Andreas Nebenführ via andreas.nebenfuehr{at}colorado.edu or at

Andreas Nebenführ
University of Colorado
MCD Biology
Boulder, CO 80309-0347
USA
phone +1-303-492-8893
fax +1-303-492-7744

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