Supplemental video material.
Chloroplast phenotypes in leaf mesophyll cells from
transgenic Arabidopsis plants with high levels of AtFtsZ1-1 protein. Cells were viewed under a microscope with a
60x oil immersion objective and the chroroplasts visualized by autofluorescence
of chlorophyll, using a Texas Red filter set (excitation 535-585 nm, emission
607-682 nm). A z-series of optical
sections, with 0.5 µm steps between slices, was videocaptured using NIH Image
software. Image stacks were then
rotated and projected (brightest point method) and the projections saved as a
movie. Bars = 25 µm.
A) Cell with severe chloroplast phenotype. The giant
chloroplast 'sheet' is wrapped around the cell. QuickTime
Video
B) 'Noodle-like' chloroplast phenotype. Two cells are shown, each cell has several
narrow, long chloroplasts.
QuickTime
Video