Plant Physiol.
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Supplemental video material.

 

Chloroplast phenotypes in leaf mesophyll cells from transgenic Arabidopsis plants with high levels of AtFtsZ1-1 protein.  Cells were viewed under a microscope with a 60x oil immersion objective and the chroroplasts visualized by autofluorescence of chlorophyll, using a Texas Red filter set (excitation 535-585 nm, emission 607-682 nm).  A z-series of optical sections, with 0.5 µm steps between slices, was videocaptured using NIH Image software.  Image stacks were then rotated and projected (brightest point method) and the projections saved as a movie. Bars = 25 µm.

A) Cell with severe chloroplast phenotype. The giant chloroplast 'sheet' is wrapped around the cell. QuickTime Video JPEG Image

B) 'Noodle-like' chloroplast phenotype.  Two cells are shown, each cell has several narrow, long chloroplasts. 

QuickTime Video JPEG Image

 





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