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Plant Physiol, January 2002, Vol. 128, pp. 9-12 Access to the Maize Genome: An Integrated Physical and Genetic Map1Department of Agronomy (E.C., G.D., J.G., M.M., M.P., H.S.-V.), Division of Biological Sciences (K.C., E.L.), and Department of Computer Engineering/Computer Science (S.C.), University of Missouri, Columbia, Missouri; Agricultural Research Service, United States Department of Agriculture, Columbia, Missouri (E.C., M.M., M.P.); Clemson University, Clemson, South Carolina (C.S., R.W.); and University of Georgia, Athens, Georgia (A.P.)
Crop plant research is poised to
make revolutionary strides including the following: cloning
target genes based on their function and/or their position in the
genome; documenting all genes and their interplay; defining and
exploring all the existing genetic diversity in a species; and using
functional information and syntenic relationships of genes in closely
related species to extrapolate gene function in crop plants. The
challenge, however, is to develop a set of comprehensive and systematic
resources to facilitate these research endeavors. Genomic resources in
maize (Zea mays) will undergird sequencing of the
maize genome and will complement and contribute to research in the
cereals, other grasses, and other crop plants.
For maize, developing genomics tools means facing the daunting
realities of size and complexity. At approximately 2,500 megabases, the
maize genome is comparable in size to that of humans, and the
complexity is likely to be greater because of the abundance of multiple
families of repetitive elements. Thus, gaining access to the maize
genome is best tackled by following Goethe's advice to seek entry to
the whole by going to its parts:
Willst du ins Unendliche schreiten,
Geh nur im Endlichen nach allen Seiten.
To efficiently take a genome apart and put it back together
requires a combination of genetic and physical mapping. In principle, genetic mapping serves to subdivide and order the genome by crossing over and recombining parts, whereas physical mapping allows ordering of
genomic fragments (parts) by determining the overlap among them.
Accordingly, the components of the public effort to develop resources
to access the complete maize genome are to produce a high-resolution
genetic map densely populated with markers; to produce, fingerprint,
and assemble a deep-coverage library of bacterial artificial
chromosomes (BACs) into physical map segments; and through molecular
markers to integrate the genetic and physical maps. The tools that are
being built in maize will provide a scaffold upon which to hang the
sequence and the gene constitution of the genome and will link the
sequence to the collected efforts of the maize genetics community over
the past century.
A core resource essential for developing an integrated
genetic/physical map for maize is a densely marked, high-resolution genetic map. We have constructed a >1850 marker map for the intermated B73/Mo17 (IBM) population (Davis et al., 2001 In addition to the map per se, numerous related resources are provided
to the maize researcher. Seeds of the IBM lines can be obtained from
the Maize Genetics Cooperation Stock Center (Urbana, IL). A collateral
resource resulting from map development is numerous additional genetic
markers. These markers include novel single copy RFLP probes, markers
for Mutator insertion sites, and new SSR primer sets. Links to
obtaining clones for hybridization probes, screening images for RFLP
probes, primer and screening information for 1,800 maize SSR loci, and
mapscore data for the IBM population are available from the Maize
Genome Database (MaizeDB, http://www.agron.missouri.edu/). Twelve
hundred SSR loci were developed by our group (Sharopova et al., 2001 We have undertaken a number of initiatives promoting the use of the IBM
map as a community resource. A subset of 94 IBM lines has been
identified for general community use. We distribute DNA of the 94 lines
in microtitre plate format for individual investigators to map their
genes much as radiation hybrid panel DNA is available for
mammalian gene mapping. We have implemented Web entry for submission of
map score data, from which the resulting locus positions are returned
to the investigator. These features are intended to provide the maize
community at large with resources to contribute to generating
comprehensive information on maize gene map position.
As a first step in producing a physical map, we constructed three
genomic DNA libraries in BACs using DNA from the inbred line B73. B73
was selected because it is one of the parents of the genetic mapping
population; thus, markers mapped on the IBM population and used to
screen the BAC libraries could provide anchors for connecting the
genetic and physical maps. To ensure deep coverage and minimal gaps in
sequence representation in the libraries, libraries were made using
three different restriction enzymes, and each library contained
sufficient numbers of clones to provide severalfold coverage of the
haploid genome. Details of the three libraries are shown in Table
I. Together, the libraries represent
27-fold genome coverage.
To assemble the BACs into contigs, the clones are fingerprinted by
digesting with HindIII. The fingerprinting involves
fractionating the fragments on agarose gels, scanning the gel images,
digitizing them with IMAGE software (Sanger Center, UK), and analyzing
for contig formation using Fingerprint Contig (FPC) software (Soderlund et al., 2000 Accuracy and speed of contig assembly can be enhanced by screening the BAC libraries with molecular markers. Resulting BAC addresses provide important anchor points for assembling individual BACs into contigs. A variety of markers is being used to screen the BAC libraries. Core RFLP Markers The 90 core RFLP markers that serve as bin landmarks on the genetic map have been used for BAC screening. The majority (79) of the markers could be used directly to hybridize to BAC filter sets. The rest of the markers contained repetitive sequences that precluded obtaining definitive addresses, pointing to the need to develop single-copy overgo probes from these markers. Maize ESTs In a partnership of our group with DuPont (Wilmington, DE) and Incyte Genomics (Palo Alto, CA), a unigene set of approximately 10,000 maize ESTs is being used to screen clones from the HindIII and EcoRI BAC libraries. The unigene set was generated by DuPont, using the publicly available maize ESTs to seed their proprietary EST collection to define unigene clusters containing the longest possible sequences for each gene in the set. At Incyte Genomics, the unigene sequences were masked for repetitive elements, overgo probes designed for each gene, and the probes used to hybridize to filters containing BAC DNA. Sorghum Markers Overgo probes derived from sorghum genomic and cDNA clones are being used to screen the BACs. These probes were designed from sequences that hybridize across several cereal genomes, including maize. Amplified Fragment-Length Polymorphism (AFLP) and Miniature Inverted-Repeat Transposable Element (MITE) Markers An additional approach for screening the BACs is based on a
strategy recently applied to constructing an integrated map for sorghum
(Klein et al., 2000 MaizeDB serves as the clearinghouse for information about all markers and the BACs they detect. BAC address information for each marker is then incorporated via FPC into the growing BAC contig assemblies. A Java applet (WebFPC) has been created to display contigs on the web at http://www.genome.clemson.edu/projects/maize/fpc/. BAC contigs are updated monthly, and the data can be searched by individual BAC clone, marker, or contig.
Current estimates indicate that at least 50% of the maize genome
consists of complex arrays of retrotransposon-like elements and that
the majority of these repetitive elements represent a small number of
related families (San Miguel et al., 1996 It is clear the task at hand is to place large numbers of genetically mapped anchor points against the BACs to both coalesce contigs and order contigs on the chromosome framework. To tie the assembled BAC contigs to the genetic map, the BACs must be screened with genetically mapped markers. The 90 core RFLP markers define the bin boundaries on the genetic map and set a framework for the integrated map. Many of the AFLP and MITE markers detected by screening the BAC pools are polymorphic in the IBM population and serve as genetic anchors. In addition, the sorghum markers are useful anchoring tools because they have all been mapped in sorghum, most have been mapped in maize, and many cross-hybridize to DNA from other cereals such as rice (Oryza sativa), sugarcane (Saccharum officinarum), and Pennisetum glaucum. Thus, these markers will not only help connect the BAC contigs to the maize map, but because of the colinearity of cereal genomes, they will also facilitate creation of comparative maps. Of the 10,000 ESTs that will be used to screen the BAC libraries, we anticipate anchoring 1,000 contigs by EST and SSR markers currently on the IBM map. Many of these locations were determined by mapping SSRs that were derived from the public EST sequences. We are initiating development of 2,000 single-nucleotide polymorphism markers derived from the ESTs. Once these global anchoring approaches draw the majority of contigs to the genetic framework, directed sequencing of BACs and BAC ends will be used to derive the source sequence for development of additional single-nucleotide polymorphism markers to attempt to bring the physical map to closure. At MaizeDB, a BLAST server has been implemented to allow users to compare sequences of interest to all public maize sequences (including those in the unigene set) to return a map location if known. The output of such a query is a bin location, BLAST score, and database links for more information, e.g. MaizeDB link to map details; CUGI link to BAC contigs; GenBank; and ZmDB (Zea mays database at Iowa State University) links for sequence and clone information. Maps can be viewed at MaizeDB in three ways. For any single map,
chromosome-specific views are available. For simultaneously looking at
two genetic maps, a comparative map viewer has been developed by
enhancing GIOT software obtained from the Rice Genome Project (Japan).
This viewer shows side-by-side comparisons of similar regions of
selected versions of maize maps and can be expanded to include
comparisons of maize to sorghum and rice. For comparing the maize
genetic map to the developing physical map The genomic resources being developed for maize will serve as a skeleton upon which to hang the sequence and the gene constitution of the genome, and will link the sequence to a century of accumulated genetic studies by members of the maize genetics community. Genomic and genetic knowledge in other cereals, other grasses, and other plant species will complement the resources in maize and will be enhanced in concert with them. MaizeDB can be accessed at http://www.agron.missouri.edu/, where maps, probes, primers, screening images, and lab protocols are presented.
Suggestions on the manuscript from Sue Wessler, University of Georgia, are appreciated. We are grateful for the advice and contributions of our External Advisory Committee, Sue Wessler, chair, University of Georgia; Vicki Chandler, University of Arizona; Joe Ecker, Salk Institute; Stan Letovsky, Cereon Genomics; and Antoni Rafalski, DuPont, for this project.
Received October 18, 2001; accepted October 18, 2001. 1 This research is supported by the National Science Foundation Plant Genome Program (grant no. DBI 9872655).
* Corresponding author; e-mail CoeE{at}missouri.edu; fax 573-882-2768.
www.plantphysiol.org/cgi/doi/10.1104/pp.010953.
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STRATEGIES TO ACCESS A...
THE GENETIC MAP
Johann Wolfgang von Goethe
12. Once all fingerprint data
have been collected, the contigs will be edited manually to ensure
accuracy and consistency.
