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BIOENERGETICS AND PHOTOSYNTHESIS

Proton Transport in Maize Tonoplasts Supported by Fructose-1,6-Bisphosphate Cleavage. Pyrophosphate-Dependent Phosphofructokinase as a Pyrophosphate-Regenerating System¹

Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941–590, RJ, Brazil

	ABSTRACT

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
The energy derived from pyrophosphate (PP_i) hydrolysis is used to pump protons across the tonoplast membrane, thus forming a proton gradient. In a plant's cytosol, the concentration of PP_i varies between 10 and 800 µM, and the PP_i concentration needed for one-half maximal activity of the maize (Zea mays) root tonoplast H⁺-pyrophosphatase is 30 µM. In this report, we show that the H⁺-pyrophosphatase of maize root vacuoles is able to hydrolyze PP_i (Reaction 2) formed by Reaction 1, which is catalyzed by PP_i-dependent phosphofructokinase (PFP):

Fructose-1,6-bisphosphate (F1,6BP) + Pi PPi +Fructose-6-phosphate (F6 P)	(reaction 1)
PPi 2 Pi	(reaction 2)
H+cyt H+vac	(reaction 3)
F1,6BP + H+cyt H+vac + F6P + Pi	(reaction 4)

During the steady state, one-half of the inorganic phosphate released (Reaction 4) is ultimately derived from F1,6BP, whereas PFP continuously regenerates the pyrophosphate (PP_i) hydrolyzed. A proton gradient (pH) can be built up in tonoplast vesicles using PFP as a PP_i-regenerating system. The pH formed by the H⁺-pyrophosphatase can be dissipated by addition of 20 mM F6P, which drives Reaction 1 to the left and decreases the PP_i available for the H⁺-pyrophosphatase. The maximal pH attained by the pyrophosphatase coupled to the PFP reaction can be maintained by PFP activities far below those found in higher plants tissues.

Plants, protozoans, and certain bacteria have a membrane-bound H⁺-translocating inorganic pyrophosphatase (H⁺-PPase) that works as a proton pump. This enzyme couples the hydrolysis of PP_i with electrogenic translocation of protons across the membrane (Baykov et al., 1999). In plants, this enzyme is located in the tonoplast membrane, where it supports vacuolar functions, such as preventing the harmful accumulation of Ca²⁺ and Na⁺ in the cytosol and allowing storage of organic metabolites that can be recovered by the cytosol when needed by the H⁺-energized tonoplast membranes. The cytosolic PP_i concentration in plant cells is approximately 200 µM (Weiner et al., 1987; Maeshima, 2000). This concentration is sufficient to promote the maximal activity of vacuolar H⁺-PPase in plant cells (Maeshima, 2000). The PP_i concentration is determined by the balance between the rates of PP_i hydrolysis and synthesis. The hydrolysis of PP_i is catalyzed by the H⁺-PPase (Davies et al., 1993). On the other hand, PP_i is synthesized as a by-product of various biosynthetic reactions such as the synthesis of nucleic acids, polysaccharides, coenzymes, and proteins; activation of fatty acids; and isoprenoid synthesis. Under stress conditions, such as anoxia, the biosynthetic reactions are slowed down, The aim of this study was to evaluate whether the maize (Zea mays) root H⁺-PPase is able to bind and hydrolyze the PP_i formed by Reaction 1 catalyzed by PFP in vitro under conditions that mimic the concentrations of F1,6BP, P_i, Mg²⁺, F2,6BP, and F6P found in the plant cell cytosol (Rebeille et al., 1983; Ukaji and Ashihara, 1987; Weiner et al., 1987; Kubota and Ashihara, 1990; Davies et al., 1993; Stitt, 1998; Table I). Here, we show that the affinity of maize root H⁺-PPase for PP_i under these conditions is sufficient to shift Reaction 1 toward PP_i formation, making it possible to build up a proton gradient in the tonoplast even in the presence of very low amounts of PFP.

View larger version (13K): [in this window] [in a new window]   Figure 1. Cleavage of F1,6BP and Mg2+ dependence. The F1,6BP cleavage was dependent on MgCl2 (A) and F1,6BP (B) concentrations. The MgCl2 dependence was measured in a medium containing: 50 mM MOPS-Tris buffer (pH 7.0), 60 milliunits mL–1 mung bean (Vigna radiata) PFP, 0.1 mg mL–1 maize vacuolar microsomal protein, 100 mM KCl, 2 µM F2,6BP, and 10 mM F1,6BP (A). When the F1,6BP dependence was measured, the MgCl2 concentration was fixed at 0.6 mM(B). The reaction was performed at 30°C and started by addition of 0.1 mM Pi. Aliquots of 0.8 mL were removed, and the reaction was stopped by the addition of 0.2 mL of 50% (w/v) trichloroacetic acid. The figure shows a representative experiment. Similar results were obtained with three different vesicle preparations.

View larger version (14K): [in this window] [in a new window]   Figure 2. Membrane proton gradient supported by PFP, F1,6BP, and Pi. The assay conditions were identical to those described in Figure 1B, except that the 9-amino-6-chloro-2-methoxyacridine (ACMA; 3 µM) was present and F1,6BP, F2,6BP, and Pi concentrations are listed below for each panel. At the end of gradient formation, 1 µM carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) was added to dissipate the H+ gradient in A and B. In C, the addition of PFP activator, F2,6BP, enhanced the proton gradient formation, and the addition of 20 mM F6P dissipated it. The arrows indicate the sequential addition of: A, 25 milliunits of PFP, 2 µM F2,6BP, 2 mM Pi, and 0.6 mM F1,6BP; B, 30 µM Pi was included in the reaction medium before the sequential addition of: 25 milliunits of PFP; 4 µM F2,6BP; 0.6 mM F1,6BP, and 2 mM Pi; C, 10 milliunits of PFP, 2 mM Pi, 0.1 mM F1,6BP, 4 µM F2,6BP, and 20 mM F6P. The figure shows a representative experiment. Similar results were obtained in three different vesicle preparations.

View larger version (19K): [in this window] [in a new window]   Figure 4. PFP dependence for the formation of the membrane proton gradient. The reaction was carried out in medium containing 10 mM MOPS-Tris (pH 7.0), 3 µM ACMA, 1 mM MgCl2, 100 mM KCl, 2 µM F2,6BP, 2 mM Pi, 0.1 mg mL–1 maize vacuolar microsomal protein, and 0.6 mM F1,6BP. The initial rate of H+ pumping was taken in the linear phase of fluorescence quenching of ACMA and was expressed as percentage F per minute after the addition of 1, 3, 8, and 18 milliunits of PFP to the cuvette (2 mL) in the absence () or in the presence () of 12 mM NaF or 2 µM FCCP (). The figure shows a representative experiment. Similar results were obtained in three different vesicle preparations. The dotted line represents the maximal rate of H+ pumping measured when 0.3 mM PPi was added to the reaction mixture in the absence of the PPi-PFP regenerating system.

	RESULTS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

PFP and maize root H⁺-PPase catalyzed the release of P_i from F1,6BP (Fig. 1). There was no measurable thereby limiting the rate of PP_i formation. Thus, the use of the reaction catalyzed by PFP in the direction of PP_i formation may be an auxiliary mechanism to prevent a drop in the cytosolic levels of PP_i under the metabolic conditions of the living cell (Davies et al., 1993). As far as we know, this proposal has never been demonstrated experimentally.

The mass to action ratio measured for the substrates and products of PFP in plants is close to its K_eq value (Kubota and Ashihara, 1990). This implies that in the plant cytosol, the direction of the reaction may be determined by the [PP_i] to [P_i] ratio and that the PP_i formed at the expense of F1,6BP could ultimately be used to pump H⁺ across the tonoplast membrane as follows:

Pi+F1,6BP PPi+F6P	(reaction 1)	G° = +0.7 kcal mol-1
PPi 2Pi	(reaction 2)	G° = -5.1 kcal mol-1
H+cyt H+vac (pH = 2)	(reaction 3)	G = +1.5 kcal mol-1
F1,6BP + H+cyt F6P + Pi+H+vac	(reaction 4)	G° = -2.9 kcal mol-1

release of P_i when the two enzymes needed to regenerate PP_i or the substrates F1,6BP and P_i were omitted from the assay medium. The rate of cleavage varied with the concentration of F1,6BP (Fig. 1B), and the half-maximal rate was observed with 123 µM F1,6BP. PFP and H⁺-PPase both require Mg²⁺ for their activities (Maeshima and Yoshida, 1989; Givan, 1999; Maeshima, 2000). Magnesium was needed for the coupled reaction in Figure 1; the maximal rate of F1,6BP cleavage was obtained in the presence of 0.6 to 1 mM MgCl₂. Higher MgCl₂ concentrations were found to be inhibitory (Fig. 1A). The findings of Figure 1 indicate that the true substrate for the maize root H⁺-PPase was the very low PP_i concentration derived from Reaction 1 and that the P_i released into the medium originated from F1,6BP by the action of PFP, which continuously regenerated the PP_i hydrolyzed (Reactions 1 and 2).

The concentration of PP_i that is attained when the PP_i-regenerating reaction reaches a steady state varied depending on the pH of the medium. The rate of F1,6BP cleavage was maximal in the pH range of 6.0 to 7.0 and decreased by 50% when the pH was raised from 7.0 to 8.0 (data not shown).

Transmembrane Proton Gradient

The assay in Figure 2 shows that the energy derived from the steady-state cleavage of F1,6BP was sufficient to form and maintain a transmembrane proton gradient in tonoplast vesicles. In the presence of PFP, the addition of F2,6BP, P_i, and F1,6BP promoted a quenching of ACMA fluorescence, indicating that a proton gradient was formed across the tonoplast membrane (Fig. 2A). The gradient was abolished when 1 µM FCCP, a proton ionophore, was added to the medium (Fig. 2, A and B).

The proton gradient formed depended on the concentration of P_i present in the reaction medium (Fig. 2B). In the presence of 30 µM P_i, a small proton gradient was observed after the addition of 0.1 mM F1,6BP (Fig. 2B). Further addition of P_i (2 mM), promoted both an increase in the initial rate of proton pumping and an enhancement of the proton gradient. This result suggests that the addition of P_i to the medium displaces Reaction 1 toward PP_i formation, increasing its steady-state concentration.

The capacity of the system to form the proton gradient was highly dependent on the presence of the PFP activator F2,6BP (Fig. 2C). A slow rate of proton pumping was measured in presence of 2 mM P_i, 0.1 mM F1,6BP, and 10 milliunits of PFP. However, when 2 µM F2,6BP was added to the medium, the rate of proton pumping was accelerated by more than 2-fold. This result confirms that the rate of the PP_i formation by PFP is stimulated by F2,6BP and that the proton gradient is also dependent on the rate of PP_i formation (Fig. 2C). Finally, the transmembrane proton gradient was abolished when 20 mM F6P was added to the medium, indicating that when the equilibrium is driven toward F1,6BP formation, the steady-state concentration of PP_i available to the maize root H⁺-PPase is greatly reduced, a condition that led to a decrease of the H⁺ gradient.

Equilibrium Concentrations of PP_i Formed from F1,6BP and P_i and PP_i Dependence of Maize Root H⁺-PPase

The equilibrium concentration of PP_i formed in the reaction catalyzed by PFP varied depending on the initial concentrations of F1,6BP, P_i, and F6P. To evaluate if the range of PP_i concentrations formed in our experiments was close to that needed for one-half maximal activation of the maize root H⁺-PPase, the PP_i concentration was calculated in two ways: by using the concentrations of substrates and products found in plant cytosol (Kubota and Ashihara, 1990; Davies et al., 1993) or by using the results obtained under the conditions of the experiments of Figure 2. In addition, we measured the apparent affinity of maize root H⁺-PPase for PP_i (Fig. 3). Table II shows the PP_i concentrations that would be formed under these different conditions. These values were calculated using the K_eq of 0.31 (Kubota and Ashihara, 1990) for the reaction catalyzed by PFP.

View larger version (21K): [in this window] [in a new window]   Figure 3. PPi saturation kinetics of maize root H+-PPase. The assay medium composition was 50 mM MOPS-Tris (pH 7.0), 100 mM KCl, 0.6 mM MgCl2, 3 µM FCCP, and 0.030 mg mL–1 maize vesicle protein. The PPi concentrations are shown on the abscissa. The reaction was started by addition of vesicles. The amount of PPi hydrolyzed never exceeded 5% of the total PPi added to the medium. The kinetic parameters shown in the figure were calculated from a nonlinear regression analysis applied to the Hill equation using the program ENZFITTER. The specific activities shown are means ± SE of at least four independent preparations.

The PP_i concentration needed for one-half maximal rate activity of maize root H⁺-PPase was 3.2 x 10^–5 M (Fig. 3). This value is similar to that previously reported by Maeshima (2000), is 10-fold lower than the PP_i equilibrium concentrations calculated in Table II (conditions A and B), and is close to the equilibrium values calculated for condition C. This means that the rate of proton pumping (Fig. 2) responds readily to the equilibrium of the reaction catalyzed by PFP. The addition of 20 mM F6P (Fig. 2C) reduces the PP_i concentration to such a low level (Table II, condition D) that can no longer be used by the maize root H⁺-PPase to accumulate H⁺ inside the tonoplast vesicles.

PFP Dependence for Formation of the Transmembrane Proton Gradient

The rate of vacuolar proton pumping varied depending on the amount of PFP added to the reaction medium (Fig. 4). The proton pumping was blocked by 12 mM fluoride, an anion that inhibits the vacuolar maize root H⁺-PPase (Fig. 4, black circles). The assay condition of 2 mM P_i, 0.5 mM F1,6BP, 2 µM F2,6BP, and 18 milliunits of PFP was sufficient to stimulate the proton pumping to a level similar to that observed using 0.3 mM PP_i, a saturating concentration (Fig. 4, white circles and dotted line). The amount of PFP used in Figure 4 is very small and is close to the levels observed in antisense transformant plants in which more than 95% of the total PFP activity has been abolished (Hajirezaei et al., 1994; Nielsen and Stitt, 2001).

	DISCUSSION

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
In this report, the capacity of maize root vacuolar H⁺-PPase to use the PP_i formed at equilibrium by PFP was evaluated. It was found that in the presence of Mg²⁺, P_i, F1,6BP and F2,6BP concentrations similar to those available in plant cytosol (Rebeille et al., 1983; Ukaji and Ashihara, 1987; Weiner et al., 1987; Kubota and Ashihara, 1990; Stitt, 1998), the PP_i formed by even a small amount of PFP was sufficient to activate the vacuolar H⁺-PPase and to form a proton gradient across the tonoplast membrane. This reaction is coupled to a decrease in the F1,6BP concentration, a compound that has a lower energy of hydrolysis than PP_i (de Meis, 1989, 1993). Table II shows that the free energy change (associated with Reaction 4) is markedly negative (Table II, G_{1 + 2} varying from –2.19 to –9.68 kcal mol^–1), despite different levels of PP_i formed under the various conditions used to calculate the equilibrium PP_i concentration. Even when the PP_i concentration was as low as 3 µM (Table II, condition D), the calculated G_{1 + 2} was –2.19 kcal mol^–1, a value that indicates a favorable shift to PP_i hydrolysis. In maize roots tips, the contribution to the steady-state level to PP_i in the cytosol of only one of the reactions that produce PP_i, the formation of UDP-Glc from Glc-1 P and UTP, was estimated to be 10 µM (Roberts, 1990) a value that is more than 3 times higher than that used to obtain a value of G_{1 + 2} of –2.19 kcal mol^–1 (Table II, condition D). This means that there are no thermodynamic constraints for the pumping of protons into the vacuole lumen using F1,6BP, P_i and 20 mM F6P to achieve the equilibrium concentration of PP_i of 3 µM. The observation that the protons leak out of the vesicles when 20 mM F6P is added to the medium (Fig. 2C) is related to the decrease of the steady-state PP_i concentration to a level far below that needed by the tonoplast H⁺-PPase (Fig. 3).

Based on reported concentrations of glycolytic intermediates of plant cells (Kubota and Ashihara, 1990), the G for the formation of PP_i (Reaction 1; Kubota and Ashihara, 1990) and the G of PP_i hydrolysis (Reactions 2 and 3; de Meis, 1984; Davies et al., 1993), it was possible to calculate the overall G for the glycolytic conversion of F1,6BP to either pyruvate or PP_i and the subsequent hydrolysis of PP_i by the H⁺-PPase needed to maintain a pH of 2 units across the tonoplast membrane. From these estimates, it becomes apparent that thermodynamic pull for the utilization of F1,6BP toward H⁺ pumping (G₁ + G₂ in Fig. 5) is slightly more favorable than the formation of pyruvate (G₃ + G₄ in Fig. 5). The readily reversible PFP reaction may provide an adaptive pathway for glycolysis and gluconeogenesis in higher plant cells (Black et al., 1987). The result shown in Figure 2 suggests a new role of PFP, the formation of PP_i to be used for the formation of a proton gradient in tonoplast vesicles.

View larger version (11K): [in this window] [in a new window]   Figure 5. Free-energy changes (G) associated with the conversion of F1,6BP to build up a H+ gradient in tonoplast vesicles or with conversion to pyruvate by the glycolytic pathway. After Glc or Fru phosphorylation, the F1,6BP formed can be diverted to PPi synthesis by PPi-PFP (G1), and the PPi is hydrolyzed by H+-PPase to build up a proton gradient of 2 units of pH in the tonoplast vesicles (G2). Alternatively, the F1,6BP is converted into triose-P by aldolase (G3). The triose-P is converted by glycolytic enzymes to pyruvate (G4). G1 and G2 were calculated as shown in Table II (condition A) using concentrations in the plant cell cytosol. The G3 and G4 were calculated from glycolytic reactions described by Kubota and Ashihara (1990).

One of the possible limiting factors for the utilization of F1,6BP and P_i to form PP_i would be the amount of PFP and/or H⁺-PPase present in the cells. A simultaneous up-regulation in the levels of H⁺-PPase and PFP have been detected during anoxia in rice (Oryza sativa) seedlings (Mertens et al., 1990; Carystinos et al., 1995; Drew, 1997). Table I shows that the levels of PFP and H⁺-PPase activities detected in different plants (Hajirezaei et al., 1994; Nielsen and Stitt, 2001) were sufficient in our assays to promote the formation of a H⁺ gradient. We found that it was possible to detect a proton gradient in tonoplast vesicles even in the presence of a PFP activity similar to that observed in antisense transformant plants (Hajirezaei et al., 1994; Nielsen and Stitt, 2001). The H⁺-PPase levels used in our experiments are similar to those detected in maize roots and other plants (Table I). These data suggest that, given the low G values of Reactions 1 and 2 (Fig. 5), the low ratio in the activity levels of PFP to H⁺-PPase would limit the vacuolar proton pumping when the concentration of PFP decreases to levels below 4 milliunits g fresh weight^–1. In recent reports (Hajirezaei et al., 1994; Nielsen and Stitt, 2001) using tobacco and potato plant transformants that lead up to a 95% reduction in the PFP activity (see also Table I), there were no major changes in carbon fluxes or leaf or plant growth. Nevertheless, there was a compensatory increment in the F2,6BP levels both in tobacco leaves and potato tubers (Hajirezaei et al., 1994; Nielsen and Stitt, 2001). In our study, the levels of PFP activity are similar to those detected in tobacco and potato plant transformants (Fig. 4; Table I). This raises the possibility that under steady-state conditions in the plant cytosol, different enzymes involved in energy transduction, such as maize root vacuolar H⁺-PPase, may use phosphate compounds with a low energy of hydrolysis (de Meis, 1989, 1993; de Meis et al., 1992a, 1992b; Montero-Lomeli and de Meis, 1992; Galina et al., 1995) to perform the work that is usually associated with the consumption of higher energy phosphate compounds such as PP_i (Romero and de Meis, 1989).

	MATERIALS AND METHODS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 

Materials and Chemicals

All chemicals were obtained from Sigma (St. Louis). PP_i:Fru-6-phosphate 1-phosphotransferase was from mung bean (Vigna radiata; Sigma F-8757). FCCP was dissolved in ethanol. The amounts used were such that the ethanol concentration in the reaction mixture was never higher than 0.5% (v/v). At this concentration, ethanol had no effect on either the H⁺ gradient or PP_i hydrolysis.

Preparation of Plant Material and Isolation of Microsomes from Maize Root Cells

Maize (Zea mays) seeds were surface sterilized with sodium hypochlorite (approximately 10 min in a 3% [v/v] solution), then washed with sterile water and soaked in water for 24 h. Radicles were harvested for preparation of vesicles from seeds allowed to germinate for 5 d on wet filter paper in the dark at 28°C.

Vacuolar membrane vesicles were isolated from whole roots using differential centrifugation. Roots (approximately 100 g wet weight) were homogenized in an IKA-Euroturrax T25 basic (speed 3 for 15 s using an S25N-18G probe, Wilmington, NC) with 200 mL of an ice-cold extraction buffer containing 10% (v/v) glycerol, 0.5% (v/v) polyvinylpyrrolidone-40, 0.13% (w/v) bovine serum albumin, 5 mM EDTA, and 0.1 M Tris-HCl (pH 8.0). Just before use, 3.3 mM dithiothreitol, 150 mM KCl, and 1 mM phenylmethylsulfonyl fluoride (final concentrations) were added to the buffer. The homogenate was strained through four layers of cheesecloth and centrifuged at 8,000g for 20 min. The supernatant was centrifuged at 100,000g for 40 min. The pellet was resuspended in 80 mL of the extraction buffer and centrifuged once more at 100,000g for 40 min. The pellet was resuspended in a small volume of ice-cold buffer containing 10 mM Tris-HCl (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA, and 1 mM dithiothreitol. The vesicles were frozen under liquid N₂. Protein concentrations were determined by the method of Lowry et al. (1951).

Measurement of F1,6BP Cleavage

The cleavage of F1,6BP was measured colorimetrically at 30°C by determining the rate of liberation of P_i (Fiske and Subbarow, 1925) in reaction media containing: 50 mM MOPS-Tris buffer (pH 7.0), 60 milliunits mL^–1 mung bean PFP, 0.1 mg mL^–1 maize vacuolar microsomal protein, 100 mM KCl, 2 µM F2,6BP, 0.6 mM MgCl₂, and different F1,6BP concentrations. When the MgCl₂ dependence was measured, the F1,6BP was fixed at 10 mM. When the pH dependence was measured, 50 mM MOPS-Tris and 50 mM glycyl-Gly buffer were added (pH from 6.0–8.5), and the concentrations of MgCl₂ and F1,6BP were 1 and 5 mM, respectively. The reaction was started by the addition of 0.1 mM P_i. Aliquots of 0.8 mL were removed and the reaction was stopped by addition of 0.2 mL of 50% (w/v) trichloroacetic acid. Reaction times were adjusted so as to limit the cleavage of F 1,6 BP to <10%. Cleavage was linear with time under these conditions.

Measurement of PP_i-Dependent Proton Transport into Maize Tonoplast Vesicles

The accumulation of H⁺ by the vesicles was determined by measuring the fluorescence quenching of ACMA (Molecular Probes, Eugene, OR) using a spectrofluorimeter (model F-3010, Hitachi, Tokyo). The excitation wavelength was set at 415 nm, and the emission wavelength was set at 485 nm. The reaction was carried out in 2 mL of medium containing 10 mM MOPS-Tris (pH 7.0), 3 µM ACMA, 1 mM MgCl₂, and 100 mM KCl.

	ACKNOWLEDGMENTS

 
We are grateful to Dr. Martha Sorenson for critical reading of the manuscript and valuable suggestions.

Received May 8, 2003; returned for revision June 20, 2003; accepted June 27, 2003.

	FOOTNOTES

 
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.026633.

¹ This work was supported by Programa de Apoio a Núcleos de Excelência-PRONEX-Financiadora de Estudos e Projetos (grant), by Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant and fellowship to A.C.d.S.), and by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (grant and fellowship to W.S.d.-S.).

^* Corresponding author; e-mail galina{at}bioqmed.ufrj.br; fax 55–21–2270–8647.

	LITERATURE CITED

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
ap Rees T, Green JH, Wilson PM (1985) Pyrophosphate-fructose 6-phosphate 1-phosphotransferase and glycolysis in non-photosynthetic tissues of higher plants. Biochem J 227: 299–304[Web of Science][Medline]

Baykov AA, Cooperman BS, Goldman A, Lahti R (1999) Cytoplasmic inorganic pyrophosphatase. Prog Mol Subcell Biol 23: 127–150[Medline]

Black CC, Mustardy L, Suna SS, Kormanik PP, Xu D-P, Paz N (1987) Regulation and roles for alternative pathways of hexose metabolism in plants. Physiol Plant 69: 387–394

Carystinos GD, MacDonald HR, Monroy AF, Dhindsa RS, Poole RJ (1995) Vacuolar H⁺-translocating pyrophosphatase is induced by anoxia or chilling in seedlings of rice. Plant Physiol 108: 641–649[Abstract]

Clayton H, Ranson J, ap Rees T (1993) Pyrophosphate-fructose-6-phosphate 1-phosphotransferase and fructose 2,6-bisphosphate in the bundle sheath of maize leaves. Arch Biochem Biophys 301: 151–157[Medline]

Davies JM, Poole RJ, Sanders D (1993) The computed free-energy change of hydrolysis of inorganic pyrophosphate and ATP. Apparent significance for inorganic-pyrophosphate-driven reactions of intermediary metabolism. Biochim Biophys Acta 1141: 29–36[CrossRef]

de Meis L (1984) Pyrophosphate of high and low energy: contribution of pH, Ca²⁺, Mg²⁺, and water to free energy of hydrolysis. J Biol Chem 259: 6090–6097[Abstract/Free Full Text]

de Meis L (1989) Role of water in the energy of hydrolysis of phosphate compounds-energy transduction in biological membranes. Biochim Biophys Acta 973: 333–349[Medline]

de Meis L (1993) The concept of energy rich phosphate compounds: water, transport ATPases and entropic energy. Arch Biochem Biophys 366: 287–296

de Meis L, Grieco MA, Galina A (1992a) Reversal of oxidative phosphorylation in submitochondrial particles using glucose 6-phosphate and hexokinase as an ATP regenerating system. FEBS Lett 308: 197–201[Medline]

de Meis L, Montero-Lomelí M, Grieco MAB, Galina A (1992b) The Maxwell Demon in biological systems: use of glucose 6-phosphate and hexokinase as an ATP regenerating system by the Ca2+-ATPase of sarcoplasmic reticulum and submitochondrial particles. Ann N Y Acad Sci 671: 19–31[Medline]

Drew MC (1997) Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Annu Rev Plant Physiol Plant Mol Biol 48: 223–250[CrossRef][Web of Science][Medline]

Duff SMG, Moorhead GBG, Lefebvre DD, Plaxton WC (1989) Phosphate starvation inducible bypasses of adenylate and phosphate-dependent glycolytic enzymes in Brassica nigra suspension cells. Plant Physiol 90: 1275–1278[Abstract/Free Full Text]

Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66: 375–400[Free Full Text]

Galina A, Reis M, Albuquerque MC, Gomez-Puyou A, Gomez-Puyou MT, de Meis L (1995) Different properties of the mitochondrial and cytosolic hexokinases in maize roots. Biochem J 309: 105–112

Givan CV (1999) Evolving concepts in plant glycolysis: two centuries of progress. Biol Rev 74: 277–309[CrossRef]

Hajirezaei M, Sonnewald U, Viola R, Carlisle S, Dennis DT, Stitt M (1994) Transgenic potato plants with strongly decreased expression of pyrophosphate: fructose-6 phosphate phosphotransferase show no visible phenotype and only minor changes in metabolic fluxes in their tubers. Planta 192: 16–30[Web of Science]

Kombrink E, Kruger NJ, Beevers H (1984) Kinetic properties of pyrophosphate-fructose-6-phosphate phosphotransferase from germinating castor bean endosperm. Plant Physiol 74: 395–401[Abstract/Free Full Text]

Kubota K, Ashihara H (1990) Identification of non-equilibrium glycolytic reactions in suspension-cultured plant cells. Biochim Biophys Acta 1036: 138–142[Medline]

Lowry OH, Rosebrough JN, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193: 265–275[Free Full Text]

Maeshima M (2000) Vacuolar H⁺-pyrophosphatase. Biochim Biophys Acta 1465: 37–51[Medline]

Maeshima M, Yoshida S (1989) Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean. J Biol Chem 264: 20068–20073[Abstract/Free Full Text]

Mertens E, Larondelle Y, Hers H-G (1990) Induction of pyrophosphate: fructose 6-phosphate 1 phosphotransferase by anoxia in rice seedlings. Plant Physiol 93: 584–587[Abstract/Free Full Text]

Milner ID, Ho LC, Hall JL (1995) Properties of proton and sugar transport at the tonoplast of tomato (Lycopersicon esculentum) fruit. Physiol Plant 94: 399–410[CrossRef]

Montero-Lomeli M, de Meis L (1992) Glucose 6-phosphate and hexokinase can be used as an ATP-regenerating system by the Ca²⁺-ATPase of sarcoplasmic reticulum. J Biol Chem 267: 1829–1833[Abstract/Free Full Text]

Nielsen TH, Stitt M (2001) Tobacco transformants with strongly decreased expression of pyrophosphate: fructose-6-phosphate 1-phosphotransferase expression in the base of their young growing leaves contain much higher levels of fructose-2,6-bisphosphate but no major changes in fluxes. Planta 214: 106–116[Web of Science][Medline]

O'Brien WE, Bowien S, Wood HG (1975) Isolation and characterization of a pyrophosphate-dependent phosphofructokinase from Propionibacterium shermanii. J Biol Chem 250: 8690–8695[Abstract/Free Full Text]

Paul M, Sonnewald U, Hajirezaei M, Dennis D, Stitt M (1995) Transgenic tobacco plants with strongly decreased expression of pyrophosphatefructose-6-phosphate 1-phosphotransferase do not differ significantly from wild-type in photosynthate partitioning, plant growth or their ability to cope with limiting phosphate. Planta 196: 277–283

Rebeille F, Bligny R, Martin J-B, Douce R (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch Biochem Biophys 225: 143–148[CrossRef][Web of Science][Medline]

Roberts JKM (1990) Observation of uridine triphosphate:glucose-1-phosphate uridylyltransferase activity in maize roots tips by saturation transfer ³¹P-NMR: estimation of cytoplasmic PP_i. Biochim Biophys Acta 1051: 29–36[Medline]

Romero P, de Meis L (1989) Role of water in the energy of hydrolysis of phosphoanhydride and phosphoesters bonds. J Biol Chem 264: 7869–7873[Abstract/Free Full Text]

Smyth DA, Chevalier P (1984) Phosphate accumulation in leaves of wheat seedlings measured in leaf cell protoplasts isolated after root or foliar treatment with ortho-phosphate. Physiol Plant 61: 135–140

Stitt M (1998) Pyrophosphate as an energy donor in the cytosol of plant cells: an enigmatic alternative to ATP. Bot Acta 111: 167–175

Ukaji T, Ashihara H (1987) Metabolic regulation in plant-cell culture: XXI. Effect of inorganic phosphate on synthesis of 5-phosphoribosyl-1-pyrophosphate in cultured plant cells. Int J Biochem 11: 1127–1131[CrossRef]

Weiner H, Stitt M, Heldt HW (1987) Subcellular compartmentation of pyrophosphate and alkaline pyrophosphatase in leaves. Biochim Biophys Acta 893: 13–21.0.0[CrossRef]

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