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ENVIRONMENTAL STRESS AND ADAPTATION

Enhanced Photosynthesis and Redox Energy Production Contribute to Salinity Tolerance in Dunaliella as Revealed by Homology-Based Proteomics¹

Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany (A.J.L., A.S.); and Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel (U.P., A.K.)

	ABSTRACT

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
Salinity is a major limiting factor for the proliferation of plants and inhibits central metabolic activities such as photosynthesis. The halotolerant green alga Dunaliella can adapt to hypersaline environments and is considered a model photosynthetic organism for salinity tolerance. To clarify the molecular basis for salinity tolerance, a proteomic approach has been applied for identification of salt-induced proteins in Dunaliella. Seventy-six salt-induced proteins were selected from two-dimensional gel separations of different subcellular fractions and analyzed by mass spectrometry (MS). Application of nanoelectrospray mass spectrometry, combined with sequence-similarity database-searching algorithms, MS BLAST and MultiTag, enabled identification of 80% of the salt-induced proteins. Salinity stress up-regulated key enzymes in the Calvin cycle, starch mobilization, and redox energy production; regulatory factors in protein biosynthesis and degradation; and a homolog of a bacterial Na⁺-redox transporters. The results indicate that Dunaliella responds to high salinity by enhancement of photosynthetic CO₂ assimilation and by diversion of carbon and energy resources for synthesis of glycerol, the osmotic element in Dunaliella. The ability of Dunaliella to enhance photosynthetic activity at high salinity is remarkable because, in most plants and cyanobacteria, salt stress inhibits photosynthesis. The results demonstrated the power of MS BLAST searches for the identification of proteins in organisms whose genomes are not known and paved the way for dissecting molecular mechanisms of salinity tolerance in algae and higher plants.

Plant proteomics have encompassed a variety of species, including Arabidopsis (Mayfield et al., 2001), rice (Koller et al., 2002), maize (Zea mays; Chang et al., 2000), pea (Pisum sativum; Peltier et al., 2000), wheat (Triticum aestivum; Amiour et al., 2002), and poppy (Papaver somniferum; Decker et al., 2000). Proteomes are characterized most effectively by a combination of protein chromatography, proteolytic digestion, and peptide MS analysis (Aebersold and Goodlett, 2001; Mann et al., 2001). Genome sequences and corresponding protein sequence DBs provide a reference for the identification of proteins by MS by the correlation of masses of intact peptides (peptide mass fingerprinting, PMF) or their fragments (tandem mass spectrometry, MS/MS) with in silico processed sequences from DB entries. Conventional protein identification algorithms, such as Mascot (Perkins et al., 1999) and SEQUEST (Eng et al., 1994), are primarily capable of exact matching of analyzed peptides to DB sequences (Fenyo, 2000; Liska and Shevchenko, 2003b), thus hampering proteome characterization in many plant species with unsequenced genomes (van Wijk, 2001; Liska and Shevchenko, 2003a). We demonstrate here that this limitation can be overcome by advanced sequence-similarity DB-searching methods.

To characterize the proteome of the green alga Dunaliella salina, we applied two-dimensional (2-D) gel electrophoresis for protein separation, followed by MS and multiple DB-searching techniques (Liska and Shevchenko, 2003b). We used the conventional DB-searching algorithm, Mascot, and two sequence similarity DB-searching algorithms, MS BLAST and MultiTag, to probe protein and expressed sequence tag (EST) DBs. MS BLAST is a sequence-similarity searching tool, which utilizes redundant, degenerate, and partially inaccurate sequence candidates obtained by the interpretation of MS/MS spectra of peptides (Shevchenko et al., 2001). All sequence candidates obtained by the interpretation of all MS/MS spectra are merged into a single search query in an arbitrary order. Because of such queries, MS BLAST does not rely on E-values of reported alignments (Altschul et al., 1990). Instead, it uses an alternative scoring scheme, which is based on the precomputed threshold scores that are set conditionally on the number of retrieved high-scoring segment pairs and the total number of fragmented precursors (Habermann et al., 2004). MultiTag software (Sunyaev et al., 2003) utilizes short (2–4 amino acid residues) stretches of peptide sequences, combined with the masses of corresponding peptide fragments and the mass of intact peptides into a peptide sequence tag. The significance of hits is estimated by computing E-values of corresponding alignments, which include several error-tolerant matching peptide sequence tags (Mann and Wilm, 1994).

In an attempt to characterize the unique salinity tolerance of Dunaliella, we identified 61 proteins from three subcellular fractions: crude plasma membrane (cPm), chloroplast (chl)-soluble, and cytosol (cyt)-soluble that were up-regulated in 3 M NaCl. The induced proteins included enzymes of central metabolic pathways, such as photosynthesis, energy production, protein synthesis and turnover, and amino acid biosynthesis. Sequence-similarity protein identification techniques were essential for effective identification of more than one-half the proteins analyzed. We therefore expect the proteomics of many plants with unsequenced genomes to be more amenable to characterization than previously facilitated by conventional methods.

	RESULTS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 

Biochemical Isolation and Fractionation of Dunaliella Proteins

D. salina cells that have been cultured continuously in either 0.5 M NaCl or in 3 M NaCl were utilized for differential protein analysis. In order to increase the resolution and dynamic range of protein detection, cells were fractionated into cPm, cyt, and chl fractions. Proteins were extracted following the protocols for each fraction and resolved on 2-D gels. Figure 1, A to C, shows a representative gel for each fraction at 0.5 and 3 M NaCl, where a sample of 250 µg protein was applied. Conventional staining with Coomassie Blue revealed approximately 800 distinct spots on each gel. The comparative analysis of 2-D gel images in all fractions suggested that, upon salinity stress in 3 M NaCl, only about 10% of detectable spots changed their intensity by more than 2-fold.

View larger version (59K): [in this window] [in a new window]   Figure 1. Two-dimensional gel electrophoresis of different cellular fractions from D. salina: A, cPm; B, cyt soluble; C, chl soluble. Left, Proteins from 0.5 M NaCl cells; right, proteins from 3 M NaCl cells. Polypeptides (250 µg) were extracted and separated on 18-cm IPG strips and then applied on 11% SDS-PAGE. Gels were stained with Coomassie Brilliant Blue R-250. Numbers indicate the selected spots and refer to the numbers in Table I; the circled spots are unidentified spots.

Protein Identification by MS

From the three cellular fractions, PMF identified 9 out of 78 spots using reference protein DB sequences from Dunaliella sp. and Chlamydomonas sp., among others (Table I). The remaining 69 proteins from the three fractions were analyzed by nanoelectrospray MS/MS and Mascot protein DB searching, which identified 23 additional proteins (in one case two proteins were identified in one spot). An example of the MS and MS/MS spectra, obtained for one protein (spot C14), is presented in Figure 2. The complete set of MS/MS spectra from the analysis was further interpreted using the MS BLAST sequence-similarity protein identification approach. Amino acid sequences were predicted de novo from MS/MS spectra and assembled into modified BLAST queries for DB searching, as previously described (Shevchenko et al., 2001). MS BLAST identified 50 proteins, which included all of the proteins identified by Mascot (except one), plus 28 additional identifications (Table I).

View larger version (25K): [in this window] [in a new window]   Figure 2. MS of a protein digest and an MS/MS peptide spectrum. MS of a protein digest is shown on the left. Peaks labeled with T are trypsin autolysis peptides. An MS/MS of the doubly charged peptide precursor (m/z 564.78) is shown on the right, with an amino acid sequence produced by automated de novo interpretation. Altogether, 13 peptides were fragmented and 97 putative peptide sequences produced by automated interpretation of these MS/MS spectra were assembled into a single query and submitted for MS BLAST DB searching. Five peptides were matched to the protein sequence of EF-Tu from the heterotrophic flagellate Reclinomonas americana. Three high-scoring segment pairs—best-scoring alignments of the queried peptide sequences and corresponding peptides from the DB entry produced by a BLAST search engine—are presented in the section below. B in the peptide sequences stands for a generic trypsin cleavage site, either R or K.

The MultiTag approach for sequence-similarity identification was used as a final attempt to identify the remaining proteins because of its demonstrated enhanced sensitivity over MS BLAST (Liska et al., 2004). This technique confirmed the identification made by Mascot and subsequently missed by MS BLAST, and contributed a single new identification, which relied upon a single peptide alignment.

Salt-Induced Proteins in Dunaliella

The overall 61 identified proteins that were up-regulated by high salt represent 45 individual proteins, according to their predicted function. This difference in number results either from cross-contamination between different fractions (six cases) or from the presence of several forms of proteins in the same fraction (eight cases).

The identified proteins, grouped according to their predicted function, are presented in Table I. The two largest categories contain enzymes of central metabolic pathways involved in carbon assimilation and mobilization or in metabolic energy production. The first group comprises five major Calvin cycle enzymes, including a special form of the Rubisco large subunit (RbcL), as well as Rubisco activase, which controls photosynthetic carbon fixation. It also contains three enzymes of the reductive pentose phosphate pathway, a starch biosynthetic enzyme, and three forms of plasma membrane carbonic anhydrase, which enhances CO₂ uptake in Dunaliella at high salinity (Fisher et al., 1996). Up-regulation of Calvin cycle enzymes and of plasma membrane carbonic anhydrases might result from enhanced photosynthetic CO₂ assimilation. In the independent experiment, we measured the rate of light-dependent carbon uptake at high and low salinity. Indeed, we found that carbon uptake activity was over 2-fold faster in cells grown in 3 M NaCl than in 0.5 M NaCl (Fig. 3).

View larger version (12K): [in this window] [in a new window]   Figure 3. Effect of salt concentration on photosynthetic CO2 fixation, oxygen evolution, and dark respiration activities. Dunaliella cells from 0.5 or 3 M NaCl cultures were washed and suspended in fresh growth media. Carbon fixation was measured from accumulation of 14C-bicarbonate (white). Rates of photosynthetic oxygen evolution (black) and dark respiration (gray) activities were measured with an oxygen electrode in the light and in the dark, respectively. Control (100%) activities represent the specific activities of 0.5 M NaCl cells. Control rates of carbon fixation, oxygen evolution, and dark respiration were 61.8 ± 4.8 µM C x mg chl–1 h–1, 96.8 ± 12.3 µM O2 evolved x mg chl–1 h–1, and 33.5 ± 5 µM O2 consumed x mg chl–1 h–1. The results represent averages of three measurements.

Another group of up-regulated proteins are key enzymes in biosynthesis of various amino acids: carbamoyl phosphate synthetase (Arg), Asp kinase-homoserine DH, (Thr, Met, and Ile), 2-isopropyl malate synthase (Leu), and 3-phosphoglycerate DH (Ser, Cys, and Gly). Related to this group is Gln synthetase (Gln synthesis), which is also a key enzyme in ammonia assimilation. Enhanced biosynthesis of amino acids may suggest dynamic changes in protein synthesis and/or turnover. This notion is further supported by up-regulation of various regulatory proteins involved in protein synthesis initiation (eukaryotic initiation factor [eIF]3 = TGF- receptor-interacting protein), elongation factors (EF-Tu, EF-G), protein processing and glycosylation (GDP-Man pyrophosphorylase), and protein degradation (26S proteasome). A unique up-regulated protein is the NADH quinone reductase (NQR)- subunit homolog, a component of a bacterial redox-driven Na⁺ extrusion system, which is associated with salinity tolerance in marine bacteria (Hayashi et al., 1995). Surprisingly, we identified this subunit in the cyt-soluble fraction, but not in the plasma membrane fraction. It is conceivable that the subunit dissociated from the membrane during purification. This subunit in bacteria has only one transmembrane domain and is easily dissociated from the bacterial complex during purification (Nakayama et al., 1998).

Two categories of general stress-related proteins in plants are up-regulated in Dunaliella at 3 M NaCl: antioxidants, involved in protection against oxidative stress, and chaperones that protect proteins against denaturation under stress conditions.

	DISCUSSION

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
In this article we describe the first large-scale proteome analysis of salt up-regulated proteins in a lower plant whose genome remains largely uncharacterized. The analysis was limited mostly to major soluble proteins that are up-regulated by no less than 1.5-fold and did not account for minor proteins. We were able to identify about 80% of the selected proteins. The successful analysis was due to cellular fractionation, which enriched relatively minor up-regulated proteins, and the sequence-similarity search algorithm, MS BLAST.

Cellular Fractionation

Many of the identified proteins in the cPm fraction were soluble proteins derived from the chl or cyt. The contamination of plasma membrane preparations with soluble proteins has been observed in many previous analyses (Santoni et al., 2000) and probably results from adsorption of soluble proteins that are released during cell lysis. For example, the RbcL, which is released from the chl, was identified primarily in the plasma membrane fraction. Integral membrane proteins were not identified in the cPm fraction probably because they are underrepresented by isoelectric focusing (IEF; Santoni et al., 2000). Thylakoid membrane and integral plasma membrane proteins are currently being resolved and analyzed by different procedures. Considering these limitations, it may be expected that the overall number of salt up-regulated proteins in Dunaliella is much larger than revealed in this study.

Salinity Tolerance in Dunaliella

The identified salt-induced proteins in Dunaliella reveal an up-regulation of central metabolic networks that can shed light on the outstanding ability of this alga to survive at high salinity.

The ability of Dunaliella to maintain and enhance photosynthetic CO₂ assimilation and energy production at high salt is remarkable when compared to the physiology of plants under salt stress; plants respond to salt stress by inhibition of photosynthesis and by stimulation of photorespiration, which result primarily from the large decrease in transpiration leading to CO₂ limitation (Wingler et al., 2000). Salinity also limits the availability of CO₂ for photosynthesis in aquatic ecosystems because of the decreased solubility of CO₂ and shifts in pKs of carbonic acid and bicarbonate (Sass and Ben-Yaakov, 1977). Cyanobacteria and algae, including Dunaliella, overcome this limitation by up-regulation of plasma membrane carbonic anhydrases, which facilitate CO₂ acquisition (Booth and Beardall, 1991; Fisher et al., 1996). We identified three carbonic anhydrases in the plasma membrane fraction (A8, A12, and A14). The 60-kD protein (spot A14) is a salt-resistant carbonic anhydrase that has been extensively characterized. The 47-kD protein (spot A8) was identified as a proteolytic product of the major 60-kD protein (Bageshwar et al., 2004). The 35-kD protein (spot A12) was found to be a distinct homolog of carbonic anhydrase protein (A. Zamir, personal communication).

In this study, we show that besides carbonic anhydrase, high salt up-regulates the expression of Rubisco, Rubisco activase, and other key enzymes in the Calvin cycle (Fig. 4), suggesting that the turnover of this CO₂-assimilating pathway is stimulated at high salt. On the contrary, in plants, salt stress was reported to down-regulate transcript levels of Calvin cycle enzymes, consistent with the inhibition of photosynthetic CO₂ fixation at high salt (Table II).

View larger version (45K): [in this window] [in a new window]   Figure 4. Schematic presentation of the enhanced metabolic pathways under high salt in Dunaliella. Red arrows indicate the salt-induced enzymes identified in this work, thick arrows (black or red) show the direction of carbon flow leading to glycerol synthesis, and numbers indicate the identified enzymes according to Table I. PM, Plasma membrane; CA, carbonic anhydrase.

Why does Dunaliella enhance photosynthesis and energy utilization at high salt? We hypothesize that high salt enhances CO₂ fixation, starch mobilization, and redox energy production to enable massive biosynthesis of glycerol (Fig. 4). The internal concentration of glycerol in Dunaliella, growing in 5 M NaCl medium, is close to 7 M, and constitutes the major carbon pool under these conditions (Avron, 1992). Specifically, glycerol is produced from enhanced CO₂ assimilation in the Calvin cycle and/or from starch degradation to dihydroxyacetone phosphate, which is reduced by NADPH to yield glycerol. This interpretation is consistent with previous biochemical evidence for enhanced synthesis of glycerol in Dunaliella from starch and photosynthetic carbon assimilation (Avron, 1992). From four enzymes metabolizing glycerol through dihydroxyacetone phosphate, we identified only dihydroxyacetone kinase (Table I, C3 and B29), which converts glycerol into dihydroxyacetone phosphate (DHAP). In contrast, a proteomic analysis in yeast (Saccharomyces cerevisiae) revealed that all four enzymes, namely, glycerol-3-phosphate DH, glycerol-3-phosphate phosphatase, glycerol kinase, and dihydroxyacetone kinase, were up-regulated at high salinity (Norbeck and Blomberg, 1997). A possible reason for this discrepancy may be that in Dunaliella some of the glycerol-metabolizing enzymes are constitutive. Under optimal growth conditions (0.5–2 M NaCl), Dunaliella accumulates considerable amounts of glycerol and no significant change in the activity of the glycerol-metabolizing enzymes were observed upon salinity stress (Oren-Shamir, 1989).

The up-regulation of Glc-6-P DH and of 6-phosphogluconate DH, the two rate-limiting enzymes of the reductive pentose phosphate pathway (PPP), suggests that the PPP is enhanced at high salt in Dunaliella. This pathway is central in carbohydrate metabolism in plants and provides both reducing power and NADPH and precursors for biosynthesis of lipids, nucleotides, aromatic amino acids, and sugar derivatives. The increase of PPP activity in Dunaliella at high salt complements the up-regulation of the Calvin cycle and enhanced glycerol synthesis. The rapid consumption of glyceraldehyde-3-P DHAP in the synthesis of glycerol requires continuous replenishment of carbon intermediates, which can be provided as ribulose-5-P, the product of the PPP. It also provides NADPH, which can be utilized for reduction of DHAP. Salt stress was reported to increase the expression of Glc-6-P DH and 6-phosphogluconate DH, and to enhance the activity of the PPP in rice and wheat as well (Krishnaraj and Thorpe, 1996; Table II). These results suggest that the PPP may have a general role in diverting carbon metabolites under salt stress in plants and algae.

The up-regulation of key enzymes in amino acid biosynthesis, and of regulatory factors in protein initiation, elongation, and degradation, suggests that, in Dunaliella, high salt increases biosynthesis and turnover of proteins. However, this notion is rather ambiguous. For example, the large increase in the abundance of Gln synthetase and carbamoyl synthase may indicate either enhanced production of Gln and Arg as a source for biosynthesis of new proteins, or enhanced degradation of proteins. Elevation of Gln synthetase in plants usually reflects increased production of ammonia, whereas Arg is a storage form for nitrogen. Enhanced ammonia production in plants can result either from high photorespiration or from elevated protein degradation (Miflin and Habash, 2002). Since in Dunaliella we found no indication for enhanced photorespiration at high salt (stimulation of photosynthetic CO₂ assimilation and oxygen evolution), we speculate that the increase in the abundance of Gln synthetase reflects ammonia production from enhanced protein degradation.

The significance of the two mitochondrial protein elongation factors, EF-G and EF-Tu, is also ambiguous, since these proteins may have dual functions. Complementary to their established role in protein biosynthesis in mitochondria and chl, bacterial homologs of both proteins have chaperone properties and were proposed to protect proteins against misfolding under stress (Caldas et al., 1998, 2000). Interestingly, salt stress was reported to up-regulate transcription of chl elongation factor, EF-Tu, in plants (Salekdeh et al., 2002).

Of particular interest is the dual-function eIF3 = TGF- receptor-interacting protein (spot A10), which has been associated with stress responses and signal transduction. Homologs of eIF3 in mammals and in plants interact with plasma membrane receptors and, as such, are part of a signal transduction pathway in response to external stimuli (Chen et al., 1995; Jiang and Clouse, 2001). A fission yeast homolog of this protein, Sum1, is part of protein initiation complex 3 and was shown to be relocalized under salt or heat stress into distinct cyt domains (Dunand-Sauthier et al., 2002).

The mitochondrial processing peptidase, zinc metalloproteases, and GDP-Man pyrophosphorylase are involved in processing and glycosylation of proteins. The latter may be associated with glycosylation of major plasma membrane proteins, such as triplicated transferrin-like protein, that accumulate in Dunaliella at high salinity (Sadka et al., 1991; Fisher et al., 1997). The overall picture emerging from these results is of a dynamic remodeling of protein composition in different cellular compartments by synthesis and processing of novel proteins as well as by massive degradation of others.

The identification of a homolog of NQR- is of special interest because it can provide a clue to clarify the exceptional ability of Dunaliella to eliminate Na⁺ ions at hypersaline solutions. Previously, we demonstrated a redox-driven Na⁺ extrusion system in Dunaliella (Katz and Pick, 2001).

The functional significance of increased production of cytoskeletal proteins (tubulin) under high salt is unclear, although it may reflect strengthening of the cell cytoskeleton.

Taken together, our findings suggest that the response of the halotolerant alga Dunaliella to salinity involves up-regulation of enzymes and metabolic pathways, some of which are specific to Dunaliella and others common to plants and related organisms.

Sequence-Similarity Protein Identification by MS

This study has demonstrated that sequence-similarity protein identification by MS can identify more than twice as many proteins as conventional approaches, thus greatly enhancing the proteome analysis in the alga D. salina. Of 78 protein spots analyzed, PMF identified 11% of the proteins, DB searching with MS/MS spectra and the conventional software, Mascot, identified 30% of the proteins, and the sequence-similarity methods, MS BLAST and MultiTag, together identified 67% of the proteins: in total, 78% of analyzed protein spots were identified from 2-D gels. Moreover, MS BLAST produced higher sequence coverage than the conventional software by aligning more peptides in an error-tolerant sequence-similarity manner, increasing the confidence of protein identifications by MS. MS BLAST recognized 266 peptides in total from MS/MS spectra (averaging 5.3 peptides/identification), whereas the conventional software was able to align only 60 peptides in total to DB entries (averaging 2.6 peptides/identification). In 19 cases, MS BLAST extended Mascot sequence coverage (i.e. from 1 to 8 peptides aligned); MS BLAST averaged 6 peptides per identification for this group. In 28 additional cases, MS BLAST made identifications where Mascot was unable to produce any significant alignments, averaging 4.4 peptide alignments per identification. EST DB searching with Mascot averaged 1.75 peptides/identification in 20 cases. False-positive functional assignments based on the sequence identity may be minimized by extending sequence coverage, but only to a certain degree. Rost has demonstrated, using bioinformatics, that proteins with high sequence similarity (70%–90%) can have diverse biochemical functions (Rost, 2002). Bona fide of the protein identifications reported here stems from the fact that multiple proteins were identified and recognized to fit into coherent metabolic pathways and groups (such as the Calvin cycle, reductive pentose phosphate pathway, antioxidation, etc.).

With sequence-similarity DB-searching methods and future related developments, the proteomes of plants with unsequenced genomes will be more amenable for characterization by high-throughput MS techniques. It will enable the identification of more conserved proteins in species that are distantly related to plants with sequenced genomes, as well as more diverse homologous proteins in species such as maize, wheat, and barley, using the closely related genomic sequences of rice. Using these methods for protein identification, immediate, rapid, and effective proteome analysis will be possible in plant biochemistry in many species, without having to wait for the completion of future genomic sequencing.

	MATERIALS AND METHODS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 

Algal Material

Dunaliella salina, a green species, was obtained from the culture collection of Dr. W.H. Thomas (La Jolla, CA). Algae were cultured for several weeks in 0.5 M NaCl (control) or in 3 M NaCl (induced) media, as previously described (Katz and Avron, 1985).

Cellular Fractionation

Cellular fractionation was preformed essentially as previously described for plasma membrane preparation (Katz et al., 1986), with minor modifications (Zchut et al., 2003). Cells were harvested and washed with isotonic glycerol buffer, osmotically equivalent to the NaCl concentration in the growth medium, lysed by an osmotic shock (1:4 dilution), and followed by centrifugation at 5,000g for 15 min. The pellet contained chls and the supernatant contained cyt and cPm fractions. The supernatant was ultracentrifuged at 250,000g for 2 h. The ultracentrifugation supernatant was referred to as the cyt-soluble fraction. The pellet, which contained the cPm, was washed again with suspension buffer containing glycerol 0.5 M, Tris-MOPS 10 mM, pH 7.5, MgCl₂ 2 mM, KCl 10 mM, and ultracentrifuged as before. The ultracentrifugation pellet was resuspended in suspension buffer and referred to as cPm.

The chls were washed with chl buffer containing glycerol 0.5 M, Tris-MOPS 10 mM, pH 7.5, MgCl₂ 2 mM, and KCl 50 mM, followed by centrifugation at 3,000g for 15 min. The chls were lysed by two freezing-thawing cycles in buffer containing Tris-MOPS 10 mM, MgCl₂ 2 mM, and KCl 10 mM, followed by centrifugation at 10,000g for 30 min. The supernatant was referred to as the chl-soluble fraction, and the pellet as the chl membrane fraction.

Enzymatic Activities

For CO₂ assimilation, D. salina cells cultured for 4 to 5 d in 0.5 or 3 M NaCl were washed and suspended in fresh growth media (at 3 x 10⁶ cells mL^–1) containing 10 mM ¹⁴C-NaHCO₃ at 25°C. Cells were incubated for 30 min at saturating light or in dark. The reaction was terminated by three washes in fresh growth media at 4°C and cell pellets were treated with 5% TCA and incubated 2 h under reduced pressure to eliminate free ¹⁴CO₂. The results presented are light minus dark values. Rates of dark oxygen uptake and of light-dependent oxygen evolution activities were measured with an oxygen electrode in cells suspended in fresh growth media at 2 x 10⁶ cells mL^–1 at 24°C.

Protein Isolation and 2-D Gels

For total cell protein extraction, 1x10⁸ cells were harvested and washed in 90% glycerol + 10% NaCl medium, iso-osmotic to the growth medium. The pellet was suspended in 0.5 mL extraction buffer, containing 700 mM Suc, 100 mM KCl, 50 mM Tris-Cl, pH 8, 5 mM EDTA, 50 mM dithiothreitol (DTT), 0.5 mM phenylmethylsulfonyl fluoride, and plant protease inhibitor cocktail (Sigma P-9599; Sigma-Aldrich, St. Louis). The cells were frozen in liquid nitrogen, thawed, and sonicated twice, followed by centrifugation at 2,000g for 3 min (to get rid of cell debris). The supernatant was taken for phenol extraction.

For cPm extraction, 700 µg of protein from cPm fraction were suspended in 250 µL extraction buffer and incubated for 10 min at 4°C. Proteins from total cells and cPm were extracted by phenol as previously described (Usuda and Shimogawara, 1995). Proteins were extracted with 1.2:1 volumes of phenol to extraction buffer, mixed vigorously, and centrifuged to obtain phase separation; the phenolic upper phase was collected, and the proteins were precipitated by addition of 5 volumes 0.1 M ammonium acetate in methanol and incubated overnight at –20°C. After centrifugation at 10,000g for 20 min, the pellet was washed with ethanol and acetone and dried. Cyt- and chl-soluble fraction extraction proteins were precipitated by acetone, 1 volume of the soluble fraction was mixed with 4 volumes of cold acetone and incubated overnight at –20°C. The extraction was centrifuged at 20,000g for 20 min, and the pellet was air dried.

The pellets of all fractions were dissolved in 2-D sample buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 0.5% Triton, 5% glycerol, and 0.5% IPG buffer, and incubated for 1 h at room temperature. At this stage, a sample was taken for determination of protein concentration, using the modified Bradford-HCl assay (Ramagli and Rodrigues, 1985). Afterward, 20 mM DTT were added followed by centrifugation at 10,000g for 10 min. Samples of 360 µL containing 250 µg protein from each fraction were loaded on 18-cm IEF dry strips, pH 3 to 10 nonlinear (Amersham Biosciences AB, Uppsala), by cup loading for the total cell proteins, pH 3 to 10 NL with in-gel rehydration for the cPm, pH 4 to 7 with in-gel rehydration for the cyt- and chl-soluble fractions. The strips were subjected to IEF for 50,000 Vh using IPGphor (Amersham Biosciences). Focused gel strips were equilibrated in SDS equilibration buffer (50 mM Tris-Cl, pH 8.8, 30% glycerol, 2% SDS, 6 M urea), first with buffer containing 1% DTT (w/v) for 15 min, and afterward with buffer containing 4% iodoacetamide for 15 min. The strips were briefly washed with running buffer and loaded on top of a prepared SDS-PAGE Laemmli system, 11% acrylamide, and covered with 0.5% agarose. SDS-PAGE was run at 20°C, 70 V, 16 h using the Hoeffer Dalt system. The running buffer contained 50 mM Tris-base, 384 mM Gly, and 0.2% SDS. Gels were stained with 0.1% Coomassie Brilliant Blue R-250 in 50% methanol, 10% acetic acid (v/v). The stained gels were scanned using ImageScanner (Amersham Biosciences). The intensity of protein spots from the 3 and 0.5 M control gels were compared using ImageMaster 2-D software version 3.01 (Amersham Biosciences), and the Z3 software version 1.5 (Compugen, Tel Aviv). Comparative analyses were performed by analyzing images from three independent cultures, with at least two repetitions for each culture. The pixel intensity of each spot was normalized against the total pixel density arising from all the protein spots on the gel. Spots up-regulated more than 2-fold were selected for analysis by MS.

MS Analysis of Protein Spots

Individual protein spots were manually excised from 2-D gels and in-gel digested with trypsin as previously described (Shevchenko et al., 1996). Extracted protein digests were analyzed first by PMF on a Bruker Reflex IV matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) MS in reflectron mode using AnchorChip 384/600 targets (Bruker Daltonics, Bremen, Germany) for preparing the probes (Thomas et al., 2004).

Proteins unidentified by PMF were analyzed by nanoelectrospray MS/MS on a modified MDS Sciex QSTAR Pulsar i quadruple time-of-flight (QqTOF) instrument using uncoated borosilicate glass capillaries (1.2 mm o.d. x 0.69 mm i.d.) from Harvard Apparatus (Holliston, MA; capillaries were drawn in-house on a Sutter P-97 puller).

DB Searching

PMF were used for DB searching by Mascot against the MSDB from NCBI (February 2003), with a mass tolerance of 150 ppm; no restrictions were imposed for protein molecular weight; species selection was set to Green Plants. Sets of MS/MS spectra from the analysis of unidentified proteins were first searched by Mascot against the above DB to identify proteins with peptides identical to those existing in silico, at a precursor mass tolerance of 0.1 D and fragment ion mass tolerance of 0.05 D, as above. Mascot queries were generated from MS/MS spectra using the processing script Mascot v.1.6b2 as an extension of Bioanalyst QS software from Applied Biosystems (Foster City, CA). Mascot EST DB searching used Other Green Plants EST_others (November 27, 2002). All MS/MS spectra were then analyzed by MS BLAST against the nonredundant nrdb protein DB at http://dove.embl-heidelberg.de/Blast2/msblast.html, as previously described (Shevchenko et al., 2001). Amino acid sequences were predicted with 0.1-D tolerance for precursor masses and a 0.05-D tolerance for fragment ions using Bioanalyst QS. Queries for MS BLAST DB searches were generated from MS/MS spectra using the ProBLAST v.1.0b11 data-processing script as an extension of Bioanalyst QS (Nimkar and Loo, 2002). In cases where scripted MS BLAST methods failed to identify a protein, an MS BLAST query was generated by manual interpretation of MS/MS spectra using Bioanalyst. If the analyzed protein remained unidentified after PMF, Mascot, and MS BLAST DB searching, with queries derived automatically and manually from MS/MS data, peptide sequence tags were constructed from MS/MS spectra by manual interpretation using Bioanalyst QS for DB searching and MultiTag analysis of search results (Sunyaev et al., 2003).

	ACKNOWLEDGMENTS

 
The authors acknowledge Dr. Henrik Thomas (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany) for MALDI-TOF assistance for PMF, Yariv Spector for 2-D gel preparation assistance, Tal Varsano for preliminary development of sample preparation, and Professor Gad Galili for advice and fruitful discussions.

Received February 4, 2004; returned for revision May 31, 2004; accepted June 2, 2004.

	FOOTNOTES

 
¹ This work was supported by The Ministry of Commerce in Israel (MAGNET program), by The Avron-Minerva Center for Photosynthesis, and by Nature Beta Technologies, Eilat, Israel.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.039438.

^* Corresponding author; e-mail adriana.katz{at}weizmann.ac.il; fax 972–8–9344118.

	LITERATURE CITED

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