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First published online October 29, 2004; 10.1104/pp.104.052274 Plant Physiology 136:3670-3681 (2004) © 2004 American Society of Plant Biologists Characterization of a Xyloglucan Endotransglucosylase Gene That Is Up-Regulated by Gibberellin in Rice1,[w]National Institute of Agrobiological Sciences, Tsukuba 305–8602, Japan (A.J., G.Y., H.N., H.I., S.K.); University of Tsukuba, Tsukuba 305–8572, Japan (A.J., H.M.); and Nagoya University, Nagoya 464–8601, Japan (H.K., M.M.)
Xyloglucan endotransglucosylases/hydrolases (XTHs) that mediate cleavage and rejoining of the  (1-4)-xyloglucans of the primary cell wall are considered to play an important role in the construction and restructuring of xyloglucan cross-links. A novel rice (Oryza sativa) XTH-related gene, OsXTH8, was cloned and characterized after being identified by cDNA microarray analysis of gibberellin-induced changes in gene expression in rice seedlings. OsXTH8 was a single copy gene; its full-length cDNA was 1,298 bp encoding a predicted protein of 290 amino acids. Phylogenetic analysis revealed that OsXTH8 falls outside of the three established subfamilies of XTH-related genes. OsXTH8 was preferentially expressed in rice leaf sheath in response to gibberellic acid. In situ hybridization and OsXTH8 promoter GUS fusion analysis revealed that OsXTH8 was highly expressed in vascular bundles of leaf sheath and young nodal roots where the cells are actively undergoing elongation and differentiation. OsXTH8 gene expression was up-regulated by gibberellic acid and there was very little effect of other hormones. In two genetic mutants of rice with abnormal height, the expression of OsXTH8 positively correlated with the height of the mutants. Transgenic rice expressing an RNAi construct of OsXTH8 exhibited repressed growth. These results indicate that OsXTH8 is differentially expressed in rice leaf sheath in relation to gibberellin and potentially involved in cell elongation processes.
The plant primary cell wall is a complex and dynamic structure that plays an important role in controlling cell shape and plant morphology as a whole. Structural modification of the cell wall is important considering regulation of cell growth and differentiation. Flowering plants have type I wall in which the principal cellulose cross-linking glycans is xyloglucan and as much as 35% of the wall mass is pectin (Carpita and Gibeaut, 1993  ; Cosgrove, 1997). In the type II cell wall of the grasses and cereals, the predominant glycans that cross link the cellulose microfibrils are glucuronoarabinoxylan and (1,3)(1,4)--D-glucan (Buckeridge et al., 2004). Type II cell walls contain a relatively low amount of xyloglucan but could nevertheless be very important (Yokoyama et al., 2004). It is considered that structural changes in these networks are regulated by enzymatic modification, and therefore wall-modifying enzymes would be expected to play an important role in regulating the plasticity of the cell walls.
A class of enzymes known as xyloglucan endotransglucosylases/hydrolases (XTHs; Yokoyama and Nishitani, 2001
The advent of the genome sequencing projects has revealed the presence of multigene XTH families in various plant species, and XTH activity has been detected in a variety of plant tissues (Nishitani, 1997 Thus, the presence of XTHs with different tissue-specific expression, hormonal regulation, and/or potentially different enzymatic properties seems to be necessary for the metabolism of xyloglucan during various stages of plant growth and development. Therefore, the characterization of individual XTH genes within a single species is essential to understand their specific roles. In this study, a novel XTH gene, named OsXTH8, was identified by a cDNA microarray analysis of GA-regulated genes in rice. We describe OsXTH8 developmental and hormonal regulation and discuss its importance in growth processes of rice.
OsXTH8 Is a Novel XTH-Related Gene
A rice XTH gene named OsXTH8 was identified by a cDNA microarray analysis of GA-regulated genes in rice. In an effort to characterize the expression of OsXTH8 in rice, full-length OsXTH8 cDNA was amplified by RACE PCR and sequenced. The OsXTH8 full-length cDNA is 1,298 bp including a poly(A)+ tail, and it encodes a predicted protein of 290 amino acid residues. The deduced amino acid sequence indicated the presence of a putative signal peptide rich in hydrophobic amino acids in the N-terminal region (von Heijne, 1986 As OsXTH is a multigene family in rice, to examine the precise expression pattern of OsXTH8 gene during development and in response to hormonal treatment, 3' untranslated region (UTR) of OsXTH8 gene was used as a specific probe. To check the specificity of 3' UTR OsXTH8, genomic DNA was digested with EcoRI, XbaI, and XhoI, respectively. Membrane probed with the 3' UTR region of OsXTH8 produced a single band (Fig. 1), confirming the specificity of the 3' UTR probe.
The deduced amino acid sequence of OsXTH8 showed sequence homology and the presence of a functional motif in comparison with other rice XTHs sequences (Fig. 2). OsXTH8 showed a sequence identity of 43%, 46%, 33%, and 38% to OsXTR1, OsXTR2, OsXTR3, and OsXTR4 (Uozu et al., 2000 ), respectively. XTHs contain a conserved sequence (DEIDFEFLG) that matches the bacterial endo--1, 3-1, 4-glucanase (Borriss et al., 1990) and has been suggested as the catalytic center for the XTH family. The corresponding sequence in OsXTH8 has two amino acid substitutions (DEIDIEFMG) compared to the consensus sequence.
To find the evolutionary relationships of OsXTH8 with XTHs of other plant species, a phylogenic tree was generated using full-length protein sequences. The analysis revealed that XTHs could be loosely grouped into four distinct groups (Fig. 3), as reported by other authors (Nishitani, 1997 ; Campbell and Braam, 1999a; Catala et al., 2001). Group 1 contains genes that share a high level of sequence identity among different species and that are expressed in young developing tissues (Catala et al., 1997; Shimizu et al., 1997; Akamatsu et al., 1999; Takano et al., 1999). Group 2 comprises XTH genes from several species showing diverse patterns of expression and response to hormonal or mechanical stimuli, including touch-inducible, flooding-response, BR-inducible, and fruit ripening-related XTHs (Catala et al., 2001). Group 3 represents a divergent group of XTHs, including NXG1 from nasturtium (Tropaeolum majus) that can act as xyloglucan hydrolase and transglycosylase (de Silva et al., 1993). OsXTH8 has the highest homology to sequences in Group 4, consisting of well-characterized barley (Hordeum vulgare) genes, HvPM2 and HvPM5, which are up-regulated by GA in barley leaf sheaths and leaves (Smith et al., 1996). Monocot members of group 4 revealed to have two substitutions (DEIDIEFMG) compared to the consensus sequence (DEIDFEFLG). The presence of different amino acid residues in the putative catalytic region may attribute to unique enzymatic activity of OsXTH8.
Alternative Organ- and Cell Type-Expression Profiles of OsXTH8
It has been shown that different members of XTH gene family are specifically regulated by various physiological and environmental stimuli (Xu et al., 1996 To examine the tissue specificity of OsXTH8, total RNAs from rice callus, root, leaf blade, and leaf sheath were hybridized with OsXTH8-specific DNA probe. A strong signal was detected in leaf sheaths but weak or no signal was observed in leaf blades, roots, and calli (Fig. 4A). These expressions were enhanced by GA3 treatment (Fig. 4A). When 1-month-old seedlings were used to characterize the expression in leaf sheath, the expression was mainly found in the three basal parts of leaf sheath (Fig. 4B). Enhanced expression of OsXTH8 in the third part of 1-month-old rice seedling, which corresponded to the second internode of leaf sheath, compared to two basal parts of leaf sheath showed that OsXTH8 is differentially expressed in leaf sheath.
In situ hybridization was performed using the basal part (culm) of 2-week-old rice seedlings to learn more about the expression pattern. On hybridization with gene-specific OsXTH8 antisense probe, the cross and longitudinal sections of culm tissue revealed an accumulation of OsXTH8 mRNA in shoot apex meristem, vascular tissues, and young leaves (Fig. 5A). Although the expression did not seem to be delimited to specific cell types, significant hybridization was observed in large and small vascular bundles of leaf sheath and peripheral cylinder of the vascular bundles and fibers in the nodal region (Fig. 5A). No significant signal was visible when sense probe was used.
To further characterize the spatial distribution patterns of OsXTH8 gene expression, 2,325 bp of the OsXTH8 upstream region from the proposed first translational start site was fused to the -glucuronidase (GUS) reporter gene. This fusion gene was introduced into rice cells, and transgenic plants were regenerated. Putative OsXTH8::GUS transgenic plants were screened by PCR. Only PCR-confirmed, transgenic lines were used for GUS staining and GUS assay. To assess whether the GUS staining patterns were consistent with the result of in situ hybridization, similar tissue sections of leaf sheath were used. Figure 5B shows the GUS expression pattern driven by OsXTH8 promoter in the basal part of leaf sheath and young nodal roots of 2-week-old rice seedlings. In the case of leaf sheath, strong GUS staining was observed in shoot apex meristem and vascular bundles, very much similar to the result of in situ hybridization (Fig. 5, A and B). Microscopic observation of vascular bundles revealed GUS expression in vascular bundle sheath and mesotomic sheath surrounding xylem and phloem. Stele or vascular cylinder region in young nodal roots of the coleoptile node and roots arising from nodal roots also showed GUS staining (Fig. 5B). Weak GUS staining was found in the sclerenchyma cells lining the epidermis of young leaves (Fig. 5B).
GA dose-dependent expression patterns of OsXTH8 mRNA were determined in rice leaf sheaths using different concentration of GA3 (1, 5, 10, and 50 µM). OsXTH8 expression was found to be up-regulated with the increase in GA3 concentration as there was no inhibitory effect of increase in GA3 concentration up to 50 µM; however, 5 µM GA3 induced maximum expression of OsXTH8 (Fig. 6A). To determine temporal expression patterns of OsXTH8 mRNA, leaf sheaths were treated for 1, 3, 6, 12, and 24 h. OsXTH8 mRNA accumulation in leaf sheath showed that during treatment of leaf sheath fragments with GA3, OsXTH8 expression was up-regulated and continued to increase throughout the 24-h incubation period (Fig. 6B). As 2-week-old rice seedlings were used for time-course experiment, because of the growth of leaf sheaths, increase in OsXTH8 expression in control could also be detected.
It has been shown that XTH genes are regulated by various hormones. For example, BRU1, a soybean XTH, is regulated by BR (Zurek and Clouse, 1994 ), and TCH4, an Arabidopsis (Arabidopsis thaliana) XTH gene, is up-regulated by auxin and BR (Xu et al., 1995). To characterize hormonal regulation of OsXTH8 expression, the effect of several plant hormones was examined on OsXTH8 mRNA abundance (Fig. 6C). When leaf sheaths were treated with GA3, brassinolide (BL), 6-bezyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA), GA3 up-regulated the expression of OsXTH8 and there was very little effect of other hormones. Uniconazole, which is a potent GA biosynthesis inhibitor, had an inhibitory effect on the OsXTH8 mRNA accumulation (Fig. 6C).
To understand the physiological functions of OsXTH8, its expression in rice mutants with abnormal heights was investigated. Tanginbozu is a GA-deficient semidwarf mutant and a single recessive gene controls the semidwarfism of Tanginbozu. Mutation in Tanginbozu blocks the three oxidative steps whereby ent-kaurene is converted to ent-kaurenoic acid resulting in less accumulation of active GA (Ogawa et al., 1996 To analyze whether the 2,325-bp 5'-promoter region of the OsXTH8 locus is sufficient for the induction of its expression, an independent transgenic line (Fig. 7B), which was transformed with 2,325 bp of OsXTH8 promoter fused to the GUS reporter gene, was treated with 5 µM GA3. It was observed that GA3 treatment enhanced the expression of the GUS reporter gene compared to untreated (mock) OsXTH8::GUS transgenic rice (Fig. 7B). This indicated that the 2,325-bp promoter region of OsXTH8 was sufficient for hormone-induced OsXTH8 expression. This observation was also confirmed by GUS assay using methyl-umbelliferylglucuronide as a substrate (Fig. 7C).
RNAi OsXTH8 Transgenic Plants Exhibited Altered Development To assess the effects of loss of function of OsXTH8 on rice growth and development, a 360-bp 3' UTR fragment of OsXTH8 was cloned into the pIG121-Hm vector in both sense and antisense orientations (Fig. 8A) for subsequent generation of rice RNAi transgenic plants. RNAi OsXTH8 was introduced into rice using Agrobacterium-mediated transformation. Rice plants transformed with only pIG121-Hm vector were used as control. At regeneration stage, the regeneration efficiency of the RNAi OsXTH8 transformed callus was only about 37% when compared to control transformed callus. After transformation, 29 transformants were generated, out of which 13 plants appeared like wild type and 16 exhibited altered vegetative growth. RNAi transformed plants showed various degrees of repressed growth and were 20% to 50% shorter than the control plants when they reached maturity (Fig. 8B). Three different RNAi transgenic lines (T1-1, T1-3, and T1-5) were selected for further analysis. RNAi transgenic line T1-1 appeared like control in phenotype, while the other two transgenic lines T1-3 and T1-5 had almost a 40% to 50% reduced height when compared to the control (Fig. 8E). To determine whether a relationship existed between the OsXTH8 RNA levels and the phenotype, RNA was extracted from the three transgenic lines to analyze their transcript levels compared with that in the control. The results are shown in Figure 8D, with ethidium bromide-stained rRNA to show that equal amounts of RNA were used. Two lines, T1-3 and T1-5, in which OsXTH8 transcript accumulated in very little amount, had a 40% and 50% reduction in height when compared to the control. Transgenic line T1-1 that appeared like control in phenotype had slightly reduced OsXTH8 mRNA accumulation. To assess whether the phenotype of the RNAi transgenic plants was caused by the knockout of OsXTH8 or other OsXTHs genes were also affected, these lines were tested for the expression of OsXTR1, OsXTR2, OsXTR3, and OsXTR4. It is clear from Figure 8D that there was no significant reduction in the transcript level of OsXTH genes tested. The internodes of RNAi OsXTH8 transgenic elongated almost normally (T in Fig. 8C), but three internodes (II, III, and IV) seemed to be shorter than the control plants. The apparent repressed growth of transgenic rice was attributed to the reduced elongation of these three internodes.
The existence of a family of 29 XTH genes in rice suggests that individual XTHs may exhibit distinct patterns of expression in terms of tissue specificity and responses to hormonal and environmental stimuli. The individual XTH enzymes encoded by the XTH gene family are thought to have varying kinetic properties as well as different catalytic functions, including transferase and hydrolase activities. Therefore, it is predicted that combinatorial expression of multiple XTHs is critical for a broad spectrum of plant developmental processes (Rose et al., 2002 ). In this study, we characterized the new XTH-related gene, named OsXTH8, which is highly expressed in rice leaf sheath and is up-regulated by GA3. Southern-blot analysis and database search revealed that OsXTH8 is a novel gene. To study the functional role of OsXTH8 in rice, the enzymatic activity profile of OsXTH8 must be examined.
The deduced amino acid sequence of OsXTH8 shares high homology, and many conserved sequences, with other XTH family members. OsXTH8 also has amino acid residues peculiar to monocotyledons within the presumed catalytic region. Most XTH family members in rice and other plants have the conserved amino acid sequence DEIDFEFLG (Fig. 2), whereas the corresponding sequence in OsXTH8 is DEIDIEFMG. This conserved sequence is also found in the catalytic region of bacterial endo-
Most sequence differences among XTH proteins are found in the carboxyl terminal and, based on the relatedness of carboxyl-terminal regions, the XTHs from many plant species can be loosely organized into four groups (Campbell and Braam, 1999a
An insight into the physiological role of XTHs can be gained from their enzymatic activity, the expression pattern of XTH genes, and the activity of promoter-GUS fusions for individual genes at various developmental stages in various parts of plants. OsXTH8 is expressed in rice leaf sheath, and, less abundantly, in root, leaf blade, and callus (Fig. 4). Similarly, OsXTR1, OsXTR2, OsXTR3, and OsXTR4 were shown to express mainly in rice culm (stem), and their expression patterns in culm seem to overlap with that of OsXTH8. OsXTR1 is expressed in both the elongation and division zones of internodes, with a higher level of expression in the former. In contrast, OsXTR4 is expressed in the division zone of internodes and nodes. OsXTR2 is expressed in most organs, with the exception of well-developed leaf blades and roots. OsXTR3 expression showed a strict organ specificity in elongating stems, being notably higher in the internode elongation zone (Uozu et al., 2000
OsXTH8 expression increased in a dose- and time-dependent manner with GA3 treatment. A significant increase in OsXTH8 mRNA was observed in leaf sheath segments or intact plants with an increase in GA3 concentration, and there was no inhibitory effect associated with further increase in GA3 concentration up to 50 µM (Fig. 6A). OsXTH8 also showed a temporal increase in transcript levels when leaf sheaths were treated with GA3. Relative to controls, the earliest increase in OsXTH8 mRNA level and leaf sheath growth was detectable after 1 h of incubation with GA3, and it continued to increase up to 24 h of incubation (Fig. 6B). Transcriptional up-regulation of XTH gene expression by GA has been demonstrated in several instances (Uozu et al., 2000
Tanginbozu is a GA-deficient semidwarf mutant of rice, the phenotype of which is controlled by a single recessive gene. The mutation in Tanginbozu blocks the three oxidative steps whereby ent-kaurene is converted to ent-kaurenoic acid, resulting in less accumulation of active GA (Ogawa et al., 1996
GUS activity and histochemical localization of GUS activity showed that a 2,325-bp fragment 5' upstream of the OsXTH8-coding region was sufficient to drive and up-regulate GUS reporter gene expression in response to GA3 (Fig. 7, B and C). Computer analysis using the PLACE signal scan program, a database of plant cis-acting regulatory DNA elements (Higo et al., 1999
One way to examine the role of XTHs in plant growth and development is to increase or decrease endogenous XTHs' mRNA content by applying transgenic methodology. RNAi OsXTH8 expressed under the control of constitutive cauliflower mosaic virus (CaMV) 35S promoter produced plants with altered growth (Fig. 8B). The repression of growth in a subpopulation of RNAi transgenic rice was attributable to the reduced second, third, and fourth internodal elongation (Fig. 8C) and is consistent with the observation that in 1-month-old wild-type rice seedlings, OsXTH8 mRNA accumulation was only observed in the first three parts of the leaf sheath (Fig. 4B), which at this stage of development correspond to the third and fourth internode. As OsXTH8 is a member of a multigene family, it is possible that silencing of OsXTH8 could affect the expression of other OsXTH genes. For this reason, we selected four OsXTR genes, where OsXTR1, OsXTR2, and OsXTR4 are fairly closely related genes and OsXTR3 is distantly related gene to OsXTH8 (Yokoyama et al., 2004
Whether the repressed internode elongation in the transgenic plants is because of some discernible alteration in cell expansion or development awaits further investigation. Sakamoto et al. (2003) In conclusion, this study shows that OsXTH8 is differentially expressed in rice leaf sheath in relation to GA. Based on the specific accumulation of OsXTH8 transcripts in young rice leaf sheath and the altered phenotype of RNAi OsXTH8, it is suggested that OsXTH8 is involved in cell wall modification processes during rice growth and development.
Plant Materials and Treatments Wild-type rice (Oryza sativa L. cv Nipponbare or cv Ginbozu) and two rice mutants, Tanginbozu and Slr1, were grown under white fluorescent light (about 200 µmol m–2 s–1, 12-h light period/d) at 25°C and 75% relative humidity in a growth chamber. Leaf sheath segments of 2-week-old seedlings were floated on 10 mL of distilled water in 60 mm x 15 mm petri dishes containing BL (Fuji Chemical, Toyama, Japan), IAA, GA3, ABA, and BA uniconazole (Wako Pure Chemical, Osaka) for various times.
A cDNA microarray containing 4,000 cDNA clones randomly selected from the rice cDNA library was used. The mRNAs for microarray probes preparation were purified with an Oligotex-dT-30 mRNA purification kit (Takara, Kyoto) according to the manufacturer's instructions. mRNA samples (1 µg) prepared from leaf sheath treated with 5 µM GA3 for 24 h or water as a control, were reverse transcribed in 20-µL volume containing 1 mM Cy3 or Cy5 dCTP (Amersham Biosciences, Piscataway, NJ), anchored oligo(dT)25, random nonamer, dithiothreitol, dNTPs, and SuperScript II (Invitrogen, Carlsbad, CA). After incubation at 42°C for 2 h, the reaction was stopped, and RNA was degraded by first heating at 94°C for 3 min and then treated with NaOH at 37°C for 15 min. Fluorescently labeled probes were purified with the QIAquick PCR purification kit (Qiagen, Hilden, Germany). Probe hybridization and scanning of the hybridized microarray slide were done according to the method of Yazaki et al. (2000)
RACE-PCR was used to clone the full-length sequence of OsXTH8 using Gene Racer kit (Invitrogen). Oligo(dT) was used for first strand synthesis. PCR was performed by using Gene Racer 5' Primer and a gene-specific primer (5'-CACACCGCCCAACTGTGCAAGATGAACT-3'). The PCR product was purified and cloned into pCR 4Blunt-TOPO vector (Zero Blunt TOPO PCR Cloning Kit for Sequencing; Invitrogen). Sequencing of the full-length cDNA was accomplished for both strands using dye-labeled terminations (PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit; Applied Biosystems, Foster City, CA) and an automated DNA sequencer (model 373A; Applied Biosystems). DNA sequence was analyzed with Genetyx-WIN Version 5.1 (Software Development, Tokyo).
Rice genomic DNA was digested with EcoRI, XbaI, and XhoI, respectively, separated by 0.8% agarose gel electrophoresis, and then transferred onto Hybond+ membranes under alkaline conditions. A 3' UTR of OsXTH8, which was PCR amplified using gene-specific primers, purified from agarose gel (QIAEXII Gel Extraction Kit; Qiagen), and radiolabeled using [alpha-32P] dCTP (Amersham Biosciences) random prime labeling system (Rediprime II; Amersham Biosciences), was used as probe for Southern-blot analysis. Hybridization was performed at 42°C in an ultrasensitive hybridization buffer (ULTRAhyb, Ambion, Austin, TX) overnight. The blot was washed twice first in 2x SSC, 0.1% x SDS at 42°C for 5 min and in 0.1x SSC, 0.1% SDS at 68°C for 15 min. It was finally analyzed by the phosphor image program with the Typhoon 8600k variable imager (Amersham Biosciences).
Tissue samples were quick-frozen in liquid nitrogen. Samples (0.5 g) were ground to powder by mortar and pestle, and total RNAs were isolated according to the procedure of Chomczynski and Sacchi (1987
To amplify OsXTH8 promoter fragment, rice genomic DNA was extracted from 1-week-old seedlings, grown on Murashige and Skoog medium (Murashige and Skoog, 1962
GUS assay was conducted according to Jefferson (1987) GUS activity was measured using the fluorogenic substrate 4-methyl-umbelliferylglucuronide (Sigma, St. Louis) and measured with an MTP-100F microplate reader (Corona Electric, Ibaraki, Japan). Each assay was carried out with crude extracts containing 5 µg proteins. The relative GUS activity was calculated as the mean of triplicate experiment.
Nodal part tissues taken from 2-week-old seedlings were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde under vacuum. Fixed samples were then dehydrated through a graded ethanol series followed by a t-butanol series, and finally embedded in paraplast. Microtome sections (10 µm thick) were mounted on silicon-coated glass slides (Matsunami, Hamamatsu, Japan). Paraplast was removed through a graded ethanol series. Probes for in situ hybridization were labeled with digoxigenin11-UTP (Roche Diagnostics, Manheim, Germany). OsXTH8 expressed sequence tag pBluescript SK plasmid was either treated with XhoI and transcribed with T7 RNA polymerase (Stratagene, La Jolla, CA; antisense probe) or digested with EcoRI and transcribed with T3 RNA polymerase (Stratagene; sense probe). Immunological detection was done with an anti-digoxygenin-AP conjugate and 4-nitrobluetetrazolium (Roche Diagnostics; Kouchi and Hata, 1993
For construction of RNAi transgenic plants, OsXTH8 cDNA in the pBluescript SK+ vector was amplified by PCR in both sense and antisense directions. Sense RNAi fragment was amplified using primer pairs of 5'-GGGGTACCTTTTGAACTCGATCGATTCAAA-3' (5' side, KpnI site underlined as a linker) and 5'-GCGAGCTCTGTTCATACCTGAGAGCATAAG-3' (3'-side, ScaI site underlined as a linker). Anti-sense RNAi fragment was amplified using primer pairs of 5'-CGTCTAGATGTTCATACCTGAGAGCATAAG-3'(5' side, XbaI site underlined as a linker) CGGGATCCTTTTGAACTCGATCGATTCAAA-3' (3' side, BamHI site underlined as a linker). The resulting PCR fragments were ligated between the CaMV 35S promoter and nopaline synthase terminator in the binary vector pIG121-Hm (Ohta et al., 1990 Total RNAs were extracted from leaves of 2-month-old control and transgenic plants and subjected to northern-blot analysis. The membrane was hybridized with the same probe and condition as described in the previous northern-blot analysis section. Sequence data from this article have been deposited with the EMBL/GenBank data libraries under accession number AB110604.
We are grateful to Dr. T. Murakami and Dr. Y. Ohashi of the National Institute of Agrobiological Sciences for their technical help. We are also grateful to Dr. K. Nakamura of Nagoya University for providing pIG121-Hm vector for rice transformation, and Dr. E.E. Hood of ProdiGene for providing Agrobacterium strain EHA105. Received August 23, 2004; returned for revision September 16, 2004; accepted September 16, 2004.
1 This work was supported in part by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan. 
[w] The online version of this article contains Web-only data. Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.052274. * Corresponding author; e-mail skomatsu{at}affrc.go.jp; fax 81–29–838–7464.
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