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First published online April 22, 2005; 10.1104/pp.105.059287 Plant Physiology 138:173-183 (2005) © 2005 American Society of Plant Biologists Sanguinarine Biosynthesis Is Associated with the Endoplasmic Reticulum in Cultured Opium Poppy Cells after Elicitor Treatment1Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4 (J.A., D.A.B., P.J.F.); and School of Biological Sciences, Washington State University, Pullman, Washington 99164–4236 (V.R.F.)
Three key benzylisoquinoline alkaloid biosynthetic enzymes, (S)-N-methylcoclaurine-3'-hydroxylase (CYP80B1), berberine bridge enzyme (BBE), and codeinone reductase (COR), were localized in cultured opium poppy (Papaver somniferum) cells by sucrose density gradient fractionation and immunogold labeling. CYP80B1 catalyzes the second to last step in the formation of (S)-reticuline, the last common intermediate in sanguinarine and morphine biosynthesis. BBE converts (S)-reticuline to (S)-scoulerine as the first committed step in sanguinarine biosynthesis, and COR catalyzes the penultimate step in the branch pathway leading to morphine. Sanguinarine is an antimicrobial alkaloid that accumulates in the vacuoles of cultured opium poppy cells in response to elicitor treatment, whereas the narcotic analgesic morphine, which is abundant in opium poppy plants, is not produced in cultured cells. CYP80B1 and BBE were rapidly induced to high levels in response to elicitor treatment. By contrast, COR levels were constitutive in the cell cultures, but remained low and were not induced by addition of the elicitor. Western blots performed on protein homogenates from elicitor-treated cells fractionated on a sucrose density gradient showed the cosedimentation of CYP80B1, BBE, and sanguinarine with calreticulin, and COR with glutathione S-transferase. Calreticulin and glutathione S-transferase are markers for the endoplasmic reticulum (ER) and the cytosol, respectively. In response to elicitor treatment, large dilated vesicles rapidly developed from the lamellar ER of control cells and fused with the central vacuole. Immunogold localization supported the association of CYP80B1 and BBE with ER vesicles, and COR with the cytosol in elicitor-treated cells. Our results show that benzylisoquinoline biosynthesis and transport to the vacuole are associated with the ER, which undergoes major ultrastructural modification in response to the elicitor treatment of cultured opium poppy cells.
Alkaloids are a diverse group of approximately 12,000 low molecular weight, nitrogen-containing compounds found in about 20% of plant species. Morphine and sanguinarine are members of the large and diverse group of benzylisoquinoline alkaloids, of which more than 2,500 different structures have been identified in plants. Although morphine is a potent narcotic analgesic, it has been implicated in the stress-induced cross-linking of galacturonic-containing polysaccharides in the cell walls of opium poppy (Papaver somniferum) plants (Morimoto et al., 2001  ). By contrast, sanguinarine, which shows specific toxic effects to herbivores and microbial pathogens, is proposed to function as an inducible defense compound (Schmeller et al., 1997). Benzylisoquinoline alkaloids share a common biosynthetic pathway, beginning with the condensation of two L-Tyr derivatives to produce the central precursor (S)-norcoclaurine (Fig. 1; Samanani et al., 2004). Specific O- and N-methyltransferases convert (S)-norcoclaurine to (S)-N-methylcoclaurine (Facchini, 2001). The P450-dependent monooxygenase (S)-N-methylcoclaurine-3'-hydroxylase (CYP80B1) catalyzes the 3'-hydroxylation of (S)-N-methylcoclaurine (Pauli and Kutchan, 1998). The subsequent 4'-O-methylation of (S)-3'-hydroxy-N-methylcoclaurine yields (S)-reticuline, the last common intermediate in the biosynthesis of both sanguinarine and morphine (Facchini, 2001). The berberine bridge enzyme (BBE) catalyzes the conversion of (S)-reticuline to (S)-scoulerine, the first committed step in the sanguinarine pathway (Facchini et al., 1996b). Alternatively, (S)-reticuline can be isomerized to its (R)-epimer as the first step in the formation of morphine (Fig. 1). The NADPH-dependent enzyme codeinone reductase (COR) converts (–)-codeinone to (–)-codeine as the penultimate step in morphine biosynthesis (Unterlinner et al., 1999). Sanguinarine is produced in cultured opium poppy cells in response to treatment with a fungal-derived elicitor (Facchini et al., 1996a). By contrast, morphine accumulation has not been detected in induced or noninduced cell cultures, although both alkaloids are abundant in the plant.
Many alkaloid biosynthetic enzymes are found in subcellular compartments other than the cytosol. For example, enzymes of the monoterpenoid indole alkaloid pathway in Catharanthus roseus have been localized to the cytosol (De Luca and Cutler, 1987 ), vacuole (McKnight et al., 1991), tonoplast (Stevens et al., 1993), thylakoid membranes (De Luca and Cutler, 1987), and endoplasmic reticulum (ER; St-Pierre and De Luca, 1995). The involvement of multiple subcellular compartments is also apparent in the biosynthesis of quinolizidine alkaloids. Although the quinolizidine nucleus is synthesized in chloroplasts (Wink and Hartmann, 1982), subsequent modifications occur only after alkaloid intermediates are transported to the cytosol and mitochondria. Two acyltransferases that catalyze the acylation of (+)-p-coumaroylepilupinine and (–)-13 -tigloyloxymultifluorine were differentially localized to the cytoplasm and mitochondria, respectively, of Lupinus albus (Suzuki et al., 1996). Ultimately, quinolizidine alkaloids are thought to accumulate in the vacuoles of epidermal cells where their defensive properties can be most effective. Such complex schemes for the compartmentalization of metabolic enzymes effectively sequester toxic alkaloids and biosynthetic intermediates away from the cytosol. The subcellular trafficking of pathway intermediates also creates a potentially key level of metabolic regulation that would not be possible if enzymes and substrates diffused freely in the cytosol.
In cultured cells, five enzymes involved in the conversion of (S)-reticuline to dihydrosanguinarine have been associated with a subcellular compartment other than the cytosol. BBE and three subsequent P450-dependent monooxygenases were localized to a membrane fraction with a specific density (
The cosedimentation of some benzylisoquinoline alkaloid biosynthetic enzymes with an endomembrane fraction exhibiting a density greater than that of the ER has led to speculation that distinct alkaloid-synthesizing vesicles occur in certain cell types (Amann et al., 1986 In this study, we investigate the subcellular localization of CYP80B1, BBE, and COR as representative enzymes of benzylisoquinoline alkaloid biosynthesis in cultured opium poppy cells treated with a fungal elicitor. We show that the association of CYP80B1 and BBE with the ER is correlated with major changes in ER ultrastructure, which appear to be linked to the elicitor-induced biosynthesis of sanguinarine in cultured opium poppy cells. Suc density gradient fractionation was used to show the localization of benzylisoquinoline alkaloid biosynthetic enzymes with distinct subcellular compartments, which were identified using antibodies to CYP80B1, BBE, COR, and specific marker proteins. Immunogold labeling and electron microscopy were used to support the subcellular compartmentalization of each biosynthetic enzyme and document changes in the ultrastructure in cultured opium poppy cells in response to elicitor treatment.
CYP80B1, BBE, and COR Levels in Elicited Opium Poppy Cell Cultures A time-course experiment was performed on opium poppy cell cultures treated with Botrytis cinerea fungal homogenate to determine if CYP80B1, BBE, and COR enzyme levels change in response to elicitor treatment. Protein was extracted from control cell cultures collected immediately after elicitor treatment (i.e. 0 h) and at various time points thereafter. Western-blot analysis was performed on these extracts using anti-CYP80B1, anti-BBE, anti-COR, and anti-calreticulin antibodies (Fig. 2). CYP80B1 and BBE protein levels were not detectable in control extracts but began to increase 5 h after the addition of the elicitor (Fig. 2). Although the levels of both CYP80B1 and BBE increased in response to elicitor treatment, BBE levels peaked at 50 h, whereas CYP80B1 levels continued to rise until at least 80 h postelicitation (Fig. 2). By contrast, COR and calreticulin were detected in control cultures, and their levels did not change in response to the addition of the elicitor (Fig. 2).
Suc Density Gradient Fractionation of Elicited Opium Poppy Cell Cultures
Protein homogenates from opium poppy cell cultures treated with the elicitor for 30 h were fractionated on a 15% to 65% continuous Suc density gradient to determine the subcellular localization of CYP80B1, BBE, and COR (Fig. 3). After ultracentrifugation, 1-mL fractions were collected from the bottom of the tube, and the density of each fraction was determined. Western blots were performed on selected fractions using antibodies specific to calreticulin, glutathione S-transferase (GST), mitochondrial aldehyde dehydrogenase (RF2), and plasma membrane proton-ATPase (PM H+-ATPase), which represented markers for the ER (Campos et al., 1996
Magnesium/EDTA Shift Assay of Elicited Opium Poppy Cell Cultures
Suc density gradients were prepared with either 10 mM MgSO4 or 10 mM EDTA to distinguish between microsomal fractions containing calreticulin and plasma membrane vesicles containing PM H+-ATPase. Magnesium ions maintain the binding of ribosomes to the ER surface, thus increasing its density. Conversely, addition of the chelating agent EDTA strips ribosomes from the ER, thus decreasing its density (Lord, 1983
Ultrastructural Changes in Opium Poppy Cells after Elicitor Treatment A time-course experiment was performed to identify changes in the cellular ultrastructure of cultured opium poppy cells in response to elicitor treatment (Fig. 5). Using a standard aldehyde fixation protocol for transmission electron microscopy, the actively growing control cultured cells were seen to have a dense cytoplasm, several plastids, and many mitochondria (Fig. 5A). Within 5 h after the addition of the elicitor, some cells showed the presence of large vesicles in the cytoplasm that were not typical of control cells (Fig. 5C). After 10 h there was evidence that smaller vesicles had fused to form large vesicles (Fig. 5D), and by 50 h the movement of vesicles to the tonoplast was apparent (Fig. 5E). The larger vesicles were often seen projecting into the vacuoles (Fig. 5, D and E). Eighty hours after elicitor treatment, many cells showed an apparent hypersensitive response including disruption of the cytoplasm and the presence of multivesicular bodies (Fig. 5F). In the later time points after elicitor treatment, the abundance of a flocculent material in the vacuole also increased, although it did not stain well using aldehyde fixation (Fig. 5E).
Potassium permanganate fixation produces a high contrast of membranous structures in cells as viewed by transmission electron microscopy, and was used to better establish endomembrane dynamics within the elicitor-treated opium poppy cell cultures. Numerous organelles and a distinctive lamellar ER were evident in control cells prepared with this fixation protocol (Fig. 6A). This fixation technique clearly showed a proliferation of irregularly shaped vesicles in the cytoplasm of elicitor-treated cells, and these vesicles contained an electron-dense material (Fig. 6B). A similar electron-dense, flocculent material was seen to accumulate in the vacuole of elicitor-treated cells (Fig. 6B). Control cells contained relatively little electron-dense material in the central vacuole and the ER lumen (Fig. 6A). Examination of many cells indicated that the large vesicles in elicitor-treated cells appeared to result from dilations of lamellar ER (Fig. 6C). The vesicles were often closely associated with the tonoplast of the central vacuole (Fig. 6B). Flocculent, electron-dense material was not visible in the Golgi of either control or elicited cells (data not shown).
Cultured opium poppy cells fixed using high-pressure freezing and freeze substitution also showed the formation of large vesicles in response to elicitor treatment (Fig. 6, D to F). Membranes of the large vesicles and the tonoplast were lined with an electron-dense material in elicitor-treated cells (Fig. 6E). In control cells, the ER exhibited a lamellar structure and together with the tonoplast lacked an electron-dense membrane lining (Fig. 6D). The large, dilated ER vesicles appeared to fuse with the central vacuole since vesicles were frequently found near the tonoplast (Fig. 6E) and complete fusion was sometimes detected (Fig. 6F).
To support the localization of CYP80B1 and BBE to the ER, and COR to the cytosol, as suggested by Suc density gradient fractionation experiments (Figs. 3 and 4), antibodies specific to each protein were used for immunogold localization in opium poppy cells treated with a fungal elicitor for 50 h. CYP80B1 was associated with the periphery of the ER, and labeling was not found in association with any other cellular compartment (Fig. 7A). Label for BBE was also detected along the ER and not in other locations (Fig. 7B). While labeling was along the ER in both cases, it could not be determined if the labeled proteins were on a particular surface of the ER or in the lumen. During tissue preparation, even lumenal proteins can become adsorbed to the ER membrane, and the resolution of the technique, as used, was also not sufficient to definitively determine on which side of the membrane an immunoreactive protein might reside (Fig. 7B). COR was localized throughout the cytosol and was not associated with the ER or other organelles (Fig. 7C). Cell sections treated with preimmune serum from individual mice used to produce the anti-CYP80B1, anti-BBE, or anti-COR sera did not show labeling (Fig. 7D).
Sanguinarine Accumulation in Elicitor-Treated Opium Poppy Cell Cultures The fluorescent alkaloid sanguinarine was found to accumulate in the central vacuole of only approximately 20% of cultured opium poppy cells in response to elicitor treatment (Fig. 8). Moreover, only cells physically associated with other cultured cells were found to contain detectable amounts of sanguinarine.
The accumulation of benzylisoquinoline alkaloids in elicitor-treated opium poppy cell cultures involves the transcriptional activation of all known genes involved in the biosynthesis of (S)-reticuline and sanguinarine (Facchini and Park, 2003 ). Time-course experiments to examine the induction of CYP80B1 and BBE protein levels (Fig. 2) were in temporal agreement with the accumulation of corresponding gene transcripts (Huang and Kutchan, 2000; Facchini and Park, 2003). COR was present in noninduced cells, but did not increase in response to elicitor treatment at the transcriptional (Huang and Kutchan, 2000; Facchini and Park, 2003) or posttranslational (Fig. 2) levels. Despite the general occurrence of several morphine biosynthetic enzymes in opium poppy cell cultures (Gerardy and Zenk, 1993a, 1993b; Lenz and Zenk, 1995a, 1995b), products of this branch pathway are not produced. The inability of cultured opium poppy cells to accumulate morphine might be caused by the absence of other relevant enzymes. The silencing of COR genes in transgenic opium poppy plants resulted in the accumulation of (S)-reticuline rather than codeinone (Allen et al., 2004). Thus, the absence of a single enzyme prevents intermediates from general benzylisoquinoline alkaloid biosynthesis from entering the morphine-specific branch pathway. Nevertheless, the efficient operation of the sanguinarine pathway shows that cultured opium poppy cells possess the essential regulatory, cellular, and metabolic components to support the biosynthesis and accumulation of a highly substituted and cytotoxic benzylisoquinoline alkaloid.
Our results show that sanguinarine biosynthesis occurs in association with the ER of cultured opium poppy cells in response to elicitor treatment. CYP80B1, BBE, and sanguinarine were shown to cosediment with calreticulin by Suc density gradient fractionation (Figs. 3 and 4). The cellular fractionation-based localization of CYP80B1 and BBE to the ER was supported by the immunogold labeling of elicitor-treated opium poppy cell cultures (Fig. 7). CYP80B1 is an integral membrane protein associated with an ER-bound NAD(P)H-dependent cytochrome P450 reductase (Pauli and Kutchan, 1998
The putative improper compartmentalization of COR might reflect the inability of cultured opium poppy cells to form a complex of morphine branch pathway enzymes (Allen et al., 2004
Potassium permanganate fixation showed the accumulation of a flocculent material in ER-derived vesicles and the central vacuole after elicitor treatment (Fig. 6). The flocculent material was not proteinaceous due to the strong oxidizing properties of potassium permanganate, which results in low contrast of protein-rich constituents, such as ribosomes and the cytoskeleton (Giddings, 2003
The large vesicles in elicited opium poppy cells (Figs. 5 and 6) appeared similar in size and morphology to previously reported alkaloid-synthesizing vesicles (Amann et al., 1986
The formation and storage of sanguinarine in cultured opium poppy cells appears to involve a similar process. Our data show that elicitor treatment causes the simultaneous activation of alkaloid biosynthetic gene expression and dilation of the ER. The induced biosynthetic enzymes can be detected by western-blot analysis (Fig. 2) and immunogold labeling (Fig. 7) during, and subsequent to, the dilation of large ER-derived vesicles (Figs. 5 and 6). Immunogold labeling provides equivocal support for the association of CYP80B1 and BBE with dilated ER (Fig. 7). Dilated ER is likely present in the immunogold-labeled cells, but is not visible because vacuoles and large vesicles are typically ruptured by the aldehyde fixation of cultured opium poppy cells (data not shown). The biosynthetic enzyme- and alkaloid-laden vesicles migrate to, and fuse with, the tonoplast membrane, releasing their contents into the vacuole (Fig. 6). Alternatively, vesicles might aggregate within, or be engulfed by, small vacuoles that subsequently fuse to the central vacuole (Fig. 5). This model appears to accurately describe the cell biology of benzylisoquinoline alkaloid biosynthesis in different species (Amann et al., 1986
In the plant, benzylisoquinoline alkaloid biosynthesis has been suggested to involve sieve elements of the phloem, whereas products of the pathway are known to accumulate in laticifers (Bird et al., 2003
Opium poppy cell cultures are not physiologically homogenous since sanguinarine accumulates in only a subset of elicitor-treated cells (Fig. 8). The cell line used in this study was selected for its ability to produce copious quantities of sanguinarine in response to elicitor treatment, with up to 50% of the alkaloid secreted into the culture medium (Eilert and Constabel, 1985
Plant Cell Cultures Cell cultures of opium poppy (Papaver somniferum; cell line 2009) were maintained under fluorescent light at 23°C on Gamborg 1B5C medium consisting of B5 salts and vitamins, 100 mg L–1 myoinositol, 1 g L–1 hydrolyzed casein, 20 g L–1 Suc, and 1 mg L–1 2,4-dichlorophenoxyacetic acid. Cells were subcultured every 6 d using a 1:3 dilution of inoculum to fresh medium.
Fungal elicitors were prepared according to Eilert et al. (1985)
The opium poppy CYP80B1 (Huang and Kutchan, 2000
Antibodies were prepared from purified antigens using repeated subcutaneous injections. Antigen proteins were dialyzed against 146 mM NaCl, resuspended at a concentration of 400 µg mL–1, emulsified (1:1) with Freund's complete adjuvant, and 100 µL injected into mice. Booster injections were performed every 3 weeks until a sufficient titer was achieved.
Cultured cells were collected by vacuum filtration and ground under liquid nitrogen to a fine powder with a mortar and pestle in the presence of polyvinyl polypyrrolidone (100 mg g–1 of tissue). Ground tissues were suspended in extraction buffer (50 mM Tris-HCl, pH 7.5, 5 mM EDTA, 5 µM PMSF, and 5 mM 2-mercaptoethanol), incubated on ice for 30 min, and the supernatant collected by centrifugation at 10,000g for 10 min at 4°C. Protein concentrations were determined according to Bradford (1976)
Continuous Suc density gradients were prepared by layering 5-mL aliquots of 50 mM Tricine-NaOH, pH 7.5, solutions containing 65, 57, 45, 37, 30, 23, and 15% (w/v) Suc in thin-wall ultracentrifuge tubes (Nalgene, Rochester, NY). Layered gradients were equilibrated for 24 h at 4°C. For magnesium-shift assays, Suc solutions were supplemented with 10 mM EDTA or 10 mM MgSO4. Protoplasts were isolated by incubating 1 g (fresh weight) of cultured plant cells in 10 mL of hydrolysis buffer (400 mM mannitol, 3 mM MES-NaOH, pH 5.8), containing 2% (w/v) cellulase R-10 (Onozuka Biochemicals, Nishinomiya, Japan) and 1% (w/v) macerozyme R-10 (Onozuka), for 16 h. Protoplasts were filtered through a 44-µm nylon mesh, centrifuged at 300g, rinsed in hydrolysis buffer, and centrifuged again. The pellet was resuspended in homogenization buffer (330 mM sorbitol, 50 mM HEPES-NaOH, pH 7.0, 0.1 mM PMSF, 2 mM EDTA, and 0.005% [v/v] Nonidet P-40) to a concentration of 2 to 3 x 103 mL–1, and protoplasts were disrupted in a Potter-Elveheim tissue grinder. Seven milliliters of protoplast lysate were layered onto the Suc density gradient and centrifuged for 3 h at 100,000g. The bottom of the ultracentrifuge tube was punctured with a 24-gauge hypodermic needle and 1-mL fractions were collected. The Suc density of each fraction was determined using a refractometer (Fisher Scientific, Loughborough, Leicestershire, UK).
Cultured opium poppy cells were fixed for 10 min in 4% (v/v) glutaraldehyde in 0.1 M cacodylic buffer, pH 7, followed by 2 h in 2% (v/v) paraformaldehyde and 2.5% (v/v) glutaraldehyde in 0.1 M cocodylate buffer, pH 7.3, and 0.2 M Suc. After fixation, the cells were washed three times for 5 min in 0.1 M cocodylate buffer, pH 7.3, and 0.2 M Suc, and fixed with 2% (w/v) osmium tetroxide for 2 h. After fixation, the cells were washed three times for 5 min in water, stained with 1% (w/v) tannic acid for 2 h, washed three times for 5 min in water, and stained with 2% (w/v) uranyl acetate for 2 h. The cells were dehydrated using a 30%, 50%, 60%, 80%, 95%, and 100% (v/v) acetone series with incubations of 2 h in each solution, and infiltrated with Spurr's resin (Electron Microscopy Sciences, Fort Washington, PA) using a 1:4, 1:3, 1:2, 1:1, 2:1, and 3:1 (resin:acetone) series with incubations of 4 h in each solution. Samples were transferred to pure resin, cast into 1-mL plastic capsules, and incubated at 60°C for 16 h.
Cultured plant cells were immersed in fixative (2% potassium permanganate in 50 mM veronal buffer, pH 9.0) for 2 h and rinsed three times each for 15 min in 100 mM sodium barbituate buffer, pH 8.8. Sections were dehydrated and embedded in Spurr's resin in acetone using a 1:4, 1:3, 1:2, 1:1, 2:1, and 3:1 (resin:acetone) infiltration series with incubations of 4 h in each solution. Samples were transferred to pure resin, cast into 1-mL plastic capsules, and incubated at 60°C for 16 h.
Cultured plant cells were resuspended in 25% dextran (39,000 g mol–1; w/v) and collected on a 30-µm nylon mesh. The cell slurry was transferred to gold sample holders that were dipped in 100 mg mL–1 lecithin in chloroform and allowed to dry. The holder was immediately frozen in a Balzers HPM 010 high-pressure freezer (Bal-tec, Balzers, Liechtenstein) and transferred to liquid nitrogen for storage. Sample holders were opened under liquid nitrogen and transferred to cryosubstitution vials containing 2% (w/v) osmium tetroxide and 8% (v/v) dimethoxypropane in anhydrous acetone. Cryosubstitution was performed at –80°C for 2 to 3 d in a bath of acetone and dry ice, and then slowly warmed to –20°C over 12 h, 4°C over 4 h, and room temperature over 4 h. After rinsing several times in acetone, the samples were removed from the sample holders and infiltrated in Spurr's resin in acetone using a 1:3, 1:1, and 3:1 (resin:acetone) infiltration series with incubations of 4 h in each solution. Samples were transferred twice to pure resin for 24 h, cast into 1-mL plastic capsules, and incubated at 60°C for 16 h.
Nickel grids (200 mesh; Electron Microscopy Sciences) were washed in acetone for 5 min, ethanol for 5 min, and allowed to dry. A 0.2% (v/v) Formvar solution was prepared in dichloroethane and filtered. A glass slide was dipped into fresh Formvar solution, allowed to dry for 2 min at room temperature, and immersed in distilled water to allow the Formvar film to float to the surface. Cleaned grids were carefully placed onto the Formvar film, and the coated grids were collected with a strip of Parafilm and allowed to dry at room temperature.
Specimen blocks were trimmed to 1 mm2 and sectioned to a thickness of 70 to 120 nm according to silver-gold refraction using 6.35-mm glass knives, cut at a 45° angle, on an Ultracut E ultramicrotome (Reichert-Jung, Vienna). Section compression was reduced using chloroform fumes.
Grids were blocked in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl (TBS) containing 2% (w/v) BSA for 1 h, incubated with the primary antibody in a humid chamber for 2 h, and rinsed three times for 15 min each in TBS containing 2% (w/v) BSA and twice for 10 min each in TBS. Grids were incubated for 1 h with 10 nm colloidal gold, goat anti-mouse IgG (Jackson Immunochemicals, West Grove, PA), and rinsed three times for 10 min each in TBS and twice for 10 min each in water. Grids were dried on filter paper before staining.
Cells fixed in osmium tetroxide and potassium permanganate were stained in 2% (w/v) uranyl acetate, 1% (w/v) lead citrate for 10 min in each solution. Immunogold-labeled grids were stained in a 4:1 2% (w/v) uranyl acetate:2% (w/v) potassium permanganate solution for 5 min. Grids were viewed at 75 kV using a Hitachi 7000X transmission electron microscope (Tokyo).
Fluorescent tissues were viewed using a Leica DM RXA2 microscope (Leica Microsystems, Wetzlar, Germany) and images acquired with a Retiga EX digital camera (Qimaging, Burnaby, Canada). Deconvolution was performed using Open Lab version 2.09 (Improvision, Coventry, UK). Sequence data from this article have been deposited with the EMBL/GenBank data libraries under accession numbers AF191772 (CYP80B1), AF025430 (BBE), and AF108432 (COR).
We thank John Rogers (Washington State University) for the calreticulin antibodies, Cornelia Ullrich (Technische Universität Darmstadt) for the PM H+-ATPase antibodies, and Patrick Schnable (Iowa State University) for the RF2 antibodies. We also thank Lacey Samuels (University of British Columbia) for assistance with the high-pressure freezing and freeze-substitution experiments. Received January 3, 2005; returned for revision February 13, 2005; accepted February 27, 2005.
1 This work was supported by the Natural Sciences and Engineering Research Council of Canada (grant to P.J.F.). P.J.F. holds the Canada Research Chair in Plant Biotechnology. 
2 These authors contributed equally to the paper.
3 Present address: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E2. Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.059287. * Corresponding author; e-mail pfacchin{at}ucalgary.ca; fax 403–289–9311.
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ABSTRACT
RESULTS
) of 1.14 g mL–1 (Amann et al., 1986
-D-thiogalactopyranoside. Cells were pelleted, resuspended in homogenization buffer (50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 10 µM phenylmethylsufonyl fluoride [PMSF], and 5 mM 2-mercaptoethanol), and ruptured using a French press (Spectronic Instruments, Rochester, NY). Cell debris and protein inclusion bodies were recovered by centrifugation. The rinsed pellet was solubilized in homogenization buffer containing 6 M urea, and the solution was passed through a 0.20-µm filter. Recombinant proteins were affinity purified using a Ni2+-charged HiTrap column according to manufacturer's instructions (Pharmacia Biotech, Piscataway, NJ).