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PLANTS INTERACTING WITH OTHER ORGANISMS

Organization and Metabolism of Plastids and Mitochondria in Arbuscular Mycorrhizal Roots of Medicago truncatula^1,[w]

Leibniz-Institut für Pflanzenbiochemie, Abteilung Sekundärstoffwechsel, D–06120 Halle (Saale), Germany (S.L., W.S., C.A., D.S., T.F.); and Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany (J.K.)

	ABSTRACT

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
Colonization of root cortical cells by arbuscular mycorrhizal fungi leads to marked cytological changes of plastids and mitochondria. Plastids in particular are forming tubular extensions partially connecting individual organelles in a network-like way. These cytological changes correspond to an increased need for plastid and mitochondrial products during establishment and functioning of the symbiosis. The analysis of metabolite and transcript levels in mycorrhizal and nonmycorrhizal roots from Medicago truncatula revealed concomitant changes regarding a number of metabolic pathways. Our results indicate the activation of the mitochondrial tricarboxylic acid cycle and of plastid biosynthetic pathways producing fatty acids, amino acids, and apocarotenoids. These observations provide a general overview of structural and metabolic changes of plastids and mitochondria during colonization of root cortical cells by arbuscular mycorrhizal fungi.

Nongreen plastids are important biosynthetic organelles. Metabolic pathways studied in recent years include N-assimilation (Esposito et al., 2003), starch biosynthesis (Geigenberger et al., 2004), and lipid biosynthesis (Schwender et al., 2004). There is an obvious need for a number of plastid products during the establishment and functioning of the AM symbiosis. Nevertheless, apart from apocarotenoid accumulation, no changes of plastid metabolism have been described in AM roots so far. We examined such changes by combining analyses of metabolite and transcript levels, including mitochondrial metabolism, which can supply ATP and carbon skeletons to the plastids (Mackenzie and McIntosh, 1999). We chose the AM model plant Medicago truncatula (Hause and Fester, 2004) for our analysis because the large amount of EST data available provides a good basis for the analysis of changes in transcript levels by electronic northern. The proliferation of organelles corresponding to the activation of mitochondrial catabolic and plastid anabolic pathways was analyzed by confocal laser scanning microscopy (CLSM) of AM roots containing organelles labeled with green fluorescent protein (GFP) after root transformation, mediated by Agrobacterium rhizogenes.

	RESULTS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 

Plastid and Mitochondrial Structures in the Roots of M. truncatula

CLSM analysis of M. truncatula roots transformed with suitably targeted GFP showed that high numbers of plastids and mitochondria are present within the central cylinder and only few of these organelles within the root cortex (Fig. 1, A, G, and I). In some cases, plastids were forming stromules within the central cylinder (Fig. 1D). AM colonization of roots led to the formation of arbuscules in the inner cortical cell layer (Fig. 1G) and to an increase in the numbers of plastids (Fig. 1, B and E) in the colonized cells. In single optical sections of cortical cells from noncolonized roots, we counted between 2 and 11 plastids (28 cells evaluated), and in single optical sections from colonized cells, 21 to 38 plastids could be observed. In some of these cells, individual plastids became connected by stromules (Fig. 1, C and F) resembling the plastid networks described for N. tabacum (Fester et al., 2001) and Z. mays (Hans et al., 2004). The connection of plastid metabolism and plastid shape is further demonstrated by plastids from transformed root explants, where plastids were virtually absent from the central cylinder and formed large roundish organelles in cortical cells (Fig. 1H).

View larger version (103K): [in this window] [in a new window]   Figure 1. Bright-field micrographs and green fluorescence according to CLSM analysis of plastids (A–H) and mitochondria (I–L) labeled by the GFP in mycorrhizal and nonmycorrhizal roots from M. truncatula. Longitudinal sections of nonmycorrhizal roots reveal large amounts of plastids (A) and mitochondria (I) within the central cylinder. Corresponding details show stromules of plastids (D, see arrows) and thread-like mitochondria (K, see arrows). The cross section of a mycorrhizal root from M. truncatula (G) allows a comparison between the occurrence of plastids within the central cylinder and within a colonized cell from the inner cortex (see arrows). Plastids from transformed root explants show large, roundish forms (H). Regarding the micrographs presenting colonized cells (B, C, E, F, J, and L), green fluorescence (B, C, and J) and bright-field micrographs (E, F, and L) are given separately, and arbuscules are marked by arrows. Plastids in such cells are strongly proliferating (B) and forming connecting stromules in some cases (C); mitochondria are proliferating and forming more or less clumped aggregates (J). All bars represent 50 µm, except for C and F, which represent 20 µm.

Transcript Levels of Key Metabolic Enzymes in AM Roots

A screening of The Institute for Genomic Research (TIGR) Medicago truncatula Gene Index (MtGI Release 7.0; http://www.tigr.org/tdb/tgi/mtgi/) for tentative consensus (TC) sequences connected to the metabolism of nongreen plastids, mitochondria, or to related cytosolic pathways resulted in about 700 sequences. Comparing the number of respective expressed sequence tags (ESTs) in cDNA libraries from mycorrhizal (in total 19,366 EST clones) and nonmycorrhizal (in total 18,783 EST clones) roots, we calculated likelihood ratios R = L¹/L⁰ according to Journet et al. (2002). These ratios refer to the likelihood (L¹, L⁰) of two hypotheses (H¹, H⁰) calculated from the number of EST sequences observed in different libraries. In the null hypothesis H⁰, the frequency of ESTs is constant, and different numbers of EST sequences are due to random variation. In the alternative hypothesis H¹, the frequency of EST sequences differs in the libraries examined. R-values therefore represent the probability for differential transcript levels regarding two tissues, here mycorrhizal and nonmycorrhizal roots, divided by the probability for identical levels of this transcript. Forty-six of the 700 sequences examined gave R = 1.7 and higher (Table I). Most of the annotations referred to enzymes of carbohydrate metabolism (including tricarboxylic acid [TCA] cycle and respiratory electron transport chain), followed by enzymes for the biosynthesis of amino acids and fatty acids. The percentage of TC sequences with elevated R-values was particularly high regarding carotenoid metabolism. Three TC sequences were annotated to corresponding enzymes, two of them with R = 2.6.

Transcript levels of the 35 sequences (Table I) in mycorrhizal and nonmycorrhizal roots were measured using real-time RT-PCR. Primers defining 50-bp amplicons were selected from TC regions corresponding to the putative open reading frames (see Supplemental Table II). All primers were tested by conventional PCR and produced single products of the expected size. Analysis of melting behavior of the PCR product after real-time PCR revealed the existence of single PCR products. Standard curves for the various amplicons indicated high amplification efficiencies in all cases presented. In the case of six TC sequences, we did not succeed in selecting suitable primer pairs. These sequences were excluded from the subsequent analyses. Difference of cycle threshold (c_t)-values were calculated by subtracting mean c_t-values of assays from mycorrhizal roots from the respective mean c_t-values of assays from nonmycorrhizal roots. An amplicon corresponding to the plant translation elongation factor (EF) 1 was used for internal standardization by substracting the c_t-value for this amplicon from the c_t-values of the various samples resulting in c_t-values. The c_t-values regarding this amplicon showed only a minor variation (c_t ranging from –0.25–0.45), indicating the use of comparable amounts of cDNA. The amplicon corresponding to the mycorrhiza-specific phosphate transporter (MtPT4) from M. truncatula (Harrison et al., 2002) was used as a control for the existence of a functional mycorrhizal symbiosis (Isayenkov et al., 2004) and for the presence of functionally active arbuscules in particular. c_t-Values regarding this amplicon were ranging between 8.4 (roots harvested 30 d after inoculation) and 9 or 12 (roots harvested 60 d after inoculation), indicating a high percentage of functional mycorrhizal colonization that corresponded to the microscopically determined degrees of mycorrhizal colonization (colonized roots/whole root system) of approximately 30% (roots harvested 30 d after inoculation) and 70% (roots harvested 60 d after inoculation). The group of plants showing the highest c_t-value for the MtPT4 amplicon gave the highest c_t-values for most amplicons. The plants harvested 30 d after inoculation with a colonization degree of approximately 30% and a c_t-value for the MtPT4-amplicon of 8.4, in contrast, gave the lowest c_t-values for most amplicons.

Roots harvested 90 d after inoculation showed a high degree of mycorrhizal colonization (approximately 80%), a c_t-value for the EF amplicon of 1.5, and a c_t-value for the MtPT4 amplicon of 9.5. c_t-values for the amplicons tested, however, were quite low. Only in the case of TC78514 (-carotene desaturase), TC79399 (enoyl-acyl carrier protein [ACP] reductase), and TC76985 (pyruvate decarboxylase) did c_t-values reach approximately 1.

Amplicons representing enzymes for amino acid and fatty acid biosynthesis as well as for carotenoid metabolism showed significantly and repeatedly elevated c_t-values regarding the plants harvested 60 d after inoculation. In most cases, c_t-values regarding the plants harvested 30 d after inoculation were elevated as well. c_t-values for these amplicons are summarized in Table I. Regarding amino acid biosynthesis, two of the TC sequences found are annotated to enzymes involved in the assimilation of ammonia and the production of Asn (Asp transaminase and Asn synthase), a major plant storage and transport compound for nitrogen. The third TC sequence is annotated to anthranilate phosphoribosyl transferase, catalyzing the second committed step of Trp biosynthesis. Regarding fatty acid biosynthesis, TC sequences with elevated transcript levels in AM roots have been found for a subunit of the acetyl-CoA carboxylase complex (ACCC) as well as for the ACP S-malonyl transferase and the enoyl-ACP reductase. Referring to the metabolism of carotenoids, only three TC sequences had been found by in silico analysis, two of them are characterized by R-values of 2.6 and c_t-values of about 1. These TC sequences are annotated as -carotene desaturase and as a carotenoid cleaving enzyme. The first of these enzymes is involved in providing lycopene, the precursor of most other carotenoids, and the second enzyme is responsible for the oxidative cleavage of carotenoids (Schwartz et al., 2001) leading to carotenoid degradation products comparable to those described repeatedly for AM roots (Fester et al., 2002a).

In summary, our analysis clearly shows an increase in transcript levels referring to enzymes of amino acid and fatty acid biosynthesis as well as carotenoid metabolism. Regarding carbohydrate metabolism, similar increases in transcript levels were predicted by in silico analysis, but were not observed by real-time RT-PCR.

Steady-State Levels of Metabolites in AM Roots

Steady-state levels of polar metabolites from mycorrhizal and nonmycorrhizal roots from M. truncatula harvested 40 d after inoculation with the AM fungus have been determined using gas chromatography-mass spectrometry (GC-MS) metabolite profiling analysis (Fig. 2A). Regarding polar metabolites, about 170 compounds were identified (the complete data set is given in Supplemental Table III). About 50 of these compounds accumulated to different levels in mycorrhizal and nonmycorrhizal roots, most notably various sugars and sugar alcohols (anhydrosorbitol, Fru, Fuc, galactinol, glycerol, gulose, maltose, maltotriose, mannitol, melezitose, melibiose, myo-inositol, rhamnose, Suc, trehalose, and Xyl), organic acids, and amino acids (acetylglutamate, Ala, aminoadipate, aminobutyrate, Arg, Asp, Asn, Cys, dehydroascorbate, ferulate, fumarate, Glu, Gln, indol-3-acetate, Lys, malate, maleate, Orn, Pro, pyroglutamate, Tyr, and Trp). In this publication, we focus on compounds referring to plastid and mitochondrial metabolism and on phosphate and trehalose, which are directly linked to a functional mycorrhizal symbiosis (Table II). Apart from clear increases in phosphate levels and from the presence of trehalose in AM roots, we observed significant increases in the levels of the amino acids Asp, Glu, Gln, Lys, Arg, and Cys and lower increases in the levels of Asn, Tyr, and Trp. In addition, two compounds involved in the mitochondrial TCA cycle, fumarate and malate, markedly decreased.

View larger version (36K): [in this window] [in a new window]   Figure 2. Representative GC-MS profile of polar (A) and GC-TOF-MS profile of nonpolar (B) metabolites from mycorrhizal M. truncatula root samples harvested 40 d after inoculation. Metabolites were identified using specific mass fragments and validated by the Automated Mass Spectral Deconvolution and Identification System in (A) and using authentic reference substances in (B). Regarding polar metabolites, only a part of the metabolites identified is indicated. The complete data set is given in Supplemental Table III. Regarding nonpolar metabolites, only the part of the chromatogram with signals from fatty acids is presented. A quantitative comparison of metabolite levels in mycorrhizal and nonmycorrhizal roots using ribitol (polar metabolites) and methyl nonadecanoate (nonpolar metabolites) as internal standards (IS) and referring to metabolites relevant for plastid or mitochondrial metabolism is given in Table II.

	DISCUSSION

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 
The massive proliferation of plastids in colonized root cortical cells from N. tabacum (Fester et al., 2001) and Z. mays (Hans et al., 2004) suggested a crucial role for these organelles. Increased amounts of products from plastid biosynthetic pathways (e.g. fatty acids or amino acids) are necessary to allow the formation of symbiotic structures (e.g. the periarbuscular membrane) and to support the general increase in metabolic activity. To detect corresponding metabolic changes, we examined plastid biosynthetic activity in AM roots of the model plant M. truncatula. Mitochondria were included in this analysis because they are providing ATP and carbon skeletons for plastid metabolism.

The Identification of Metabolic Changes in AM Roots

Possibly differentially accumulating transcripts were selected in a first screen by electronic northern analysis prior to a closer analysis by real-time RT-PCR. Whereas only few differentially accumulating transcripts connected to primary metabolism have been observed in AM roots using cDNA arrays for transcript profiling (Liu et al., 2003), electronic northern analysis resulted in a number of sequences that could be analyzed further.

There are a number of problems when analyzing metabolic changes in AM roots: (1) Our measurements refer to whole root systems, containing relatively small numbers of colonized cells (Fig. 1G) of varying symbiotic state. Accordingly, our analysis underestimates the actual changes regarding transcript and metabolite levels in individual colonized cells.

(2) Transcript levels referring to primary metabolism may depend on various external factors, possibly explaining the poor correlation between a number of R- and c_t-values. The TC sequences annotated to Asp transaminase and ACCC, example given, were characterized by small R-values (1.9 each) but had clearly elevated transcript levels in AM roots according to real-time RT-PCR. Other TC sequences with high R-values like ferritin (741), Suc synthase (48), or pyruvate decarboxylase (34) were apparently not induced in our plants according to real-time RT-PCR.

(3) A further problem regards the interpretation of metabolite data. Whereas the plant origin of apocarotenoids (Walter et al., 2000) and some fatty acids (Olsson, 1999) as well as the predominant fungal origin of trehalose (Douds et al., 2000) and a different set of fatty acids (Olsson, 1999) have been shown before, it is impossible to determine the biosynthetic origin of individual intermediates of carbohydrate catabolism or amino acid biosynthesis. Nevertheless, the observation of concomitant changes of metabolite and transcript levels regarding individual enzymatic steps allows the identification of metabolic pathways apparently induced in colonized roots (Fig. 3). Thus, our data provide a comprehensive view of the metabolic reactions of roots to the colonization by AM fungi.

View larger version (60K): [in this window] [in a new window]   Figure 3. Complementary changes of transcript and metabolite levels indicating the activation of mitochondrial TCA cycle and of plastid biosynthetic pathways producing fatty acids, amino acids, and apocarotenoids. Enzymatic steps referring to TC sequences with significantly more EST clones in cDNA libraries from mycorrhizal when compared to nonmycorrhizal roots (R > 1.7) are marked in green. Confirmation of differential transcript levels by real-time RT-PCR is indicated by bold red arrows or red dots. Metabolites showing increased amounts in mycorrhizal roots are marked in red, and metabolites with decreased amounts are shown in blue. Increased amounts of metabolites might partially be due to fungal biosynthetic activity (see discussion). PPP, Pentose phosphate pathway; PEP, phosphoenolpyruvate; GAP, glycerinaldehyde 3-P.

According to GC-MS analysis, the levels of amino acids such as Asp, Glu, Gln, Lys, Arg, Cys, Asn, Tyr, and Trp are increased in AM roots. The increasing levels of these metabolites are accompanied by increasing levels of plant transcripts for key biosynthetic enzymes like Asp aminotransferase, Asn synthase, and anthranilate phosphoribosyl transferase.

In the case of fatty acids, plants and AM fungi are producing different sets of metabolites. Palmitvaccenoate and vaccenoate are typical fungal fatty acids, and palmitate, linoleate, and linolenate are of predominant plant origin (Olsson, 1999). Increasing levels of both groups of metabolites are the result of active plant and fungal fatty acid biosynthesis. The activation of plant fatty acid biosynthesis is further confirmed by finding increased transcript levels for key enzymes of this pathway (acetyl-CoA carboxylase, ACP S-malonyl transferase, and enoyl-ACP reductase).

Regarding apocarotenoid biosynthesis, both fragments derived from carotenoid oxidative cleavage can be detected photometrically in M. truncatula by HPLC (Fester et al., 2002a, 2005) but were not detected by the methods used here. In addition, the AM-dependent activation of the methylerythritol-P pathway (Walter et al., 2000) and of carotenoid biosynthesis (Fester et al., 2002b) has been demonstrated for M. truncatula. These earlier reports are now supported by finding AM-responsive transcripts with sequence similarity to a further enzyme involved in carotenoid biosynthesis (-carotene desaturase) and to a carotenoid cleaving dioxygenase producing the fragments observed in AM roots (Schwartz et al., 2001; Fester et al., 2002a).

Regarding mitochondrial metabolism, we observed a decrease of fumarate and malate, two metabolites of the TCA cycle. Fumarate in particular is exclusively assigned to the TCA cycle, while malate is also involved in transport systems between the cytosol and mitochondria or plastids. The finding of reduced steady-state levels of these two compounds is indicative most likely for an increased activity of the TCA cycle. This conclusion is supported by positive in silico results for a number of respective TC sequences. Regarding the activation of other pathways of carbohydrate catabolism (glycolysis or the pentose phosphate pathway [PPP]), only few in silico and no experimental data were obtained. Because products of these pathways, however, are necessary for building up fatty acids and carotenoids, a respective activation in AM roots appears likely.

Functional Significance of the Metabolic Changes Observed

The cellular changes observed during the formation of arbuscules suggest a general activation of plant cell metabolism (Bonfante-Fasolo, 1984; Gianinazzi-Pearson, 1996). This activation results in a higher demand for most products of plastid metabolism, including nucleotides, amino acids, and fatty acids. In agreement with our findings, fatty acids can be predicted to be of particular importance. Building up the periarbuscular membrane, colonized cells are increasing twofold to fourfold the surface of their plasma membrane (Bago et al., 2000). In addition, intracellular membrane systems, e.g. plastid, mitochondrial, or endoplasmic reticulum membranes (Hause and Fester, 2004), are proliferating as well. In contrast to this, the prominent position of the biosynthetic pathway leading to Asp and Asn (Fig. 3) reflects either an increasing demand for protein biosynthesis or the ability of AM fungi to provide nitrogen to their host plants (Hodge et al., 2001) and the use of Asn as a major compound for storage and transport of nitrogen in M. truncatula. The latter process might involve plastids from the central cylinder as well as plastids from colonized root cortical cells. Finally, the functional meaning of apocarotenoid biosynthesis has not been explained so far (Strack et al., 2003), although such compounds are accumulating in AM roots from a large variety of plants, in some cases to considerable levels (Fester et al., 2002a, 2005), and the induction of respective enzymes has been shown in a number of cases (Walter et al., 2000; Fester et al., 2002b; Hans et al., 2004). Recent experiments suggest a connection of this phenomenon with the accumulation of hydrogen peroxide close to disintegrating arbuscules (Fester and Hause, 2005).

The analysis of metabolic flux and regulation in specialized nongreen plastids in recent years has provided crucial features of metabolism in these organelles. In short, the oxidative PPP can be assumed to be the main source for NADPH as has been shown in a number of studies regarding Glu biosynthesis (Bowsher et al., 1989, 1992; Wright et al., 1997; Esposito et al., 2003). The intermediates of the oxidative PPP and glycolysis have been shown to be rapidly exchangeable between plastids and the cytosol when analyzing fatty acid producing Brassica napus embryos (Schwender et al., 2004). ATP-consuming pathways, like the methylerythritol-P pathway necessary for carotenoid biosynthesis, are mainly controlled by the activity of the ATP translocator in the inner plastid envelope as has been shown for starch biosynthesis in potato (Solanum tuberosum) tubers (Geigenberger et al., 2004). Furthermore, it is tempting to speculate that phosphate provided by the fungus might have a direct influence on the various translocators situated in the inner plastid envelope and on starch biosynthesis (Douds et al., 2000), leading to a higher level of glycolytic intermediates within the cytosol. Thus, the provision of high amounts of phosphate by the fungus might be ultimately linked to an elevated amount of carbohydrates available for the fungus.

Cytological Changes during Arbuscule Formation

Regarding mitochondria, Logan and Leaver (2000) described shapes ranging from spherical to sausage- or thread-like forms for roots from Arabidopsis (Arabidopsis thaliana). In M. truncatula as in Arabidopsis, spherical forms appear to be more predominant within root cortical cells, whereas thread-like forms are abundant in the central cylinder. Nevertheless, there is a certain percentage of thread-like mitochondria in root cortical cells from M. truncatula. Upon colonization of root cortical cells, mitochondria are aggregating in the vicinity of arbuscules forming clumps similar to those reported by Logan and Leaver (2000) for cells from the Arabidopsis hypocotyl.

Plastids in the roots of M. truncatula show a wide range of structural variation: Plastids from the cortex of root explants have a very large, amyloplast-like appearance without possessing any stromules, and plastids from the root cortex of intact plants are much smaller, but apparently free of stromules as well. Stromules become visible in the case of plastids from the central cylinder, from root nodules (B. Hause, K. Demchenko, and K. Pawlowski, personal communication), and from root cortical cells colonized by AM fungi. Plastids from root nodules and from arbusculated root cortical cells do not only share structural features, but some metabolic features as well. According to a metabolite and transcript profiling project for the rhizobial interaction of L. japonicus, another member of the Fabaceae, plastids from root nodules are mainly involved in the biosynthesis of amino acids, most notably Asn, which is the major export form of nitrogen from the nodules of L. japonicus and M. truncatula (Colebatch et al., 2004). Similarities regarding this metabolic pathway are further suggested by our electronic northern analysis. cDNA libraries from mycorrhizal and nodulated roots show similar numbers of EST clones connected to Asn or Asp metabolism, which is not the case for sequences connected to fatty acid biosynthesis or apocarotenoid accumulation. Plastid stromule formation has been suggested to be linked to increased metabolic activity of plastids (e.g. Kwok and Hanson, 2004; Waters et al., 2004). Such a correlation seems to be supported by the examples presented above.

There are several possible reasons for the correlation of plastid metabolic activity and stromule formation. Stromules allow the plastids of a given cell to physically interact with each other. In the case of arbusculated cells from N. tabacum and Z. mays, plastids seem to form one large compartment, covering the growing arbuscule in a net-like appearance. In the case of M. truncatula, this interconnection is somewhat less pronounced. In addition, an increase in the area of the plastid inner envelope may be necessary for the exchange of plastid metabolites and in particular for the biosynthesis of lipophilic compounds, like fatty acids and carotenoids. A possible connection between carotenoid biosynthesis and stromule formation in AM roots is suggested by a comparison of these features in M. truncatula on one hand and in N. tabacum and Z. mays on the other. Both the activity of carotenoid biosynthesis (Fester et al., 2002a, 2002b) and of stromule formation appear to be decreased in M. truncatula when compared with these two plants.

Outlook

The use of organelle-targeted GFP and the complementary results regarding transcript and metabolite levels provided a first general overview of structural and metabolic changes of cell organelles during colonization by AM fungi. To our knowledge, the magnitude and extent of these changes are not paralleled by other biological processes involving root plastids. A detailed functional analysis of the formation of plastid networks in AM roots might reveal factors involved in signaling between nucleus and plastids, between individual plastids, and between the plant and the fungal cell. The data presented here define possible markers for such an analysis regarding metabolic changes. Similar markers for the study of structural changes are currently being studied (e.g. the plastid division protein FtsZ; Osteryoung and McAndrew, 2001) or still have to be determined (e.g. genes involved in stromule formation).

	MATERIALS AND METHODS

TOP ABSTRACT RESULTS DISCUSSION MATERIALS AND METHODS LITERATURE CITED

 

Plant Material and AM Fungus Inoculation

Barrel medic (Medicago truncatula L. Gaertn. var Jemalong) was grown in a greenhouse in pots filled with expanded clay (Lecaton, 2–5 mm particle size; Fibo Exclay Deutschland). Seven-day-old seedlings (five plants per 500-mL pot) were inoculated with the AM fungus Glomus intraradices Schenck and Smith by the application of propagules in expanded clay (isolate 49, provided by H. von Alten from the collection of the Institut für Pflanzenkrankheiten und Pflanzenschutz der Universität Hannover, Germany). Further details of plant growth conditions have been described previously (Maier et al., 1995). Roots were harvested at 30, 60, or 90 d after inoculation. Part of the root system from each pot was used for the estimation of approximate colonization percentage (colonized roots/whole root system) according to Philips and Hayman (1970) after staining with trypan blue in lactophenol. The rest of the roots was homogenized in liquid nitrogen and stored at –80°C.

Root Transformation and Microscopy Analysis

Roots of M. truncatula were transformed according to the method published by Boisson-Dernier et al. (2001) using the Agrobacterium rhizogenes strain ARquaI. A construct containing the signal peptide of spinach (Spinacia oleracea) ferredoxin NADP(H) oxidoreductase fused to a modified version of the GFP cloned in pBIN (kindly provided by R.B. Klösgen, Halle, Germany; for further description, see Marques et al., 2004) was used for targeting the GFP to plastids. A similar construct in pSMAB701 containing the signal peptide of the -subunit of the Arabidopsis (Arabidopsis thaliana) mitochondrial F₁-ATPase (kindly provided by Y. Niwa, Shizuoka, Japan; for further description, see Niwa et al., 1999) was used for targeting a modified version of the GFP (S65T-GFP) to mitochondria. Composite plants with transformed roots were selected by fluorescence stereomicroscopy (using the GFP1-filter of the MZ FLIII from Leica) before being planted into open pots filled with expanded clay and the mycorrhizal inoculum (G. intraradices Schenck and Smith). Hairy root explants were prepared by repeated subculturing of transformed roots, first in liquid culture containing 1 mg mL^–1 augmentin, then on agar plates containing the same amount of the antibiotic. The composite plants were accustomed to greenhouse conditions and harvested after approximately 8 weeks. Transformed roots were selected again by fluorescence stereomicroscopy, sliced with a razor blade, and immediately analyzed by CLSM. CLSM was performed using the LSM 510 Meta from Zeiss equipped with a Kr/Ar laser (emission at 488 nm). GFP fluorescence (filter setting: NFT545, BP505–530), orange red autofluorescence of root cell walls and arbuscules (filter setting: NFT545, LP560), as well as bright-field micrographs were collected simultaneously.

In Silico Analysis of Gene Expression

TC sequences corresponding to enzymes from various metabolic pathways were searched using the respective lists provided by the TIGR Medicago truncatula Gene Index (MtGI Release 7.0; http://www.tigr.org/tdb/tgi/mtgi/). TC and clone identification numbers are further given according to the MtGI nomenclature. The numbers of ESTs constituting the TC sequences were compared in three cDNA libraries from mycorrhizal roots (MHAM, MTAMP, MTBC; comprising 19,366 EST clones) on the one hand, and five libraries from nonmycorrhizal roots (MTBA, KVO, MTRHE, rootphos-, developing root; comprising 18,783 EST clones) on the other hand. The likelihood ratio (R) between the two hypotheses that a given gene is differentially expressed (H¹) and that it is not differentially expressed (H⁰) was calculated for every TC sequence analyzed according to Equations 4 and 8 from Journet et al. (2002). TC sequences with R = 1.7 and higher were aligned against their most similar tentative annotations as given by MtGI or as found by BLAST homology search using the BLAST algorithm (http://www.ncbi.nlm.nih.gov/BLAST/). Details of annotation are given online in Supplemental Table I. For estimating transcript levels of these TC sequences in nodulated roots, the number of respective EST clones in the libraries MtSN4, GVN, GVSN, M. truncatula R108 Mt, nodulated root, and MtBB (comprising 21,373 sequences in total) were counted.

Analysis of Transcript Steady-State Levels

Total RNA was extracted from roots using a modified protocol from Gibco BRL Life Technologies. Ground, frozen root material (100 mg) was suspended in 1 mL TriReagent (Sigma-Aldrich). After centrifugation, the supernatant was extracted with chloroform, and RNA was precipitated by adding 250 µL 0.8 M sodium citrate, 1.2 M sodium chloride, and 250 µL isopropanol to 450 µL solution. The RNA pellet was washed twice with 75% (v/v) ethanol, dissolved in 20 µL water, and analyzed photometrically and by nondenaturing agarose/ethidium bromide gel electrophoresis. cDNA was synthesized using Moloney murine virus reverse transcriptase (Promega) according to the instructions from the manufacturer.

Primers for real-time PCR analysis were designed for all TC sequences showing R-values >1.7 using Primer Express software (Applied Biosystems). Prior to primer design, the parts of the TC sequences without homology to the coding sequence of the most similar tentative annotation were deleted. When comparing primer pairs with the MtGI database, six primer pairs recognized closely related TC sequences annotated to the same enzymes. In all other cases, primer pairs recognized only the TC sequence they had been derived from. The primers used for analysis are given online in Supplemental Table II.

Real-time PCR was performed using an Applied Biosystems Prism 7000 sequence detection system according to the instructions from the manufacturer. Amplification was followed using the dye SYBR Green I in assays with a total volume of 20 µL. The quality of primers and the efficiency of the amplification were checked by conventional PCR, by analyzing the melting behavior of the PCR product after real-time PCR, and by running standard curves using five different dilutions (factor 4) of cDNA. All assays (standard curve and measurements) were run with three technical parallels. For each transcript studied, three biologically independent samples from mycorrhizal and nonmycorrhizal roots were measured, respectively, summing up to 18 assays per transcript. The data were evaluated using the comparative C_T method for relative quantitation as specified by Applied Biosystems. c_t-values were calculated by subtracting mean c_t-values of assays from mycorrhizal roots from the respective mean c_t-values of assays from nonmycorrhizal roots. The analysis was performed twice using two different plant batches harvested 60 d after inoculation. Amplicons showing high c_t-values were then analyzed again using roots harvested 30 and 90 d after inoculation, respectively. An amplicon corresponding to TC85208 (annotated to the translation elongation factor 1; 5'-AGCACCAAGCAAAGCATCCT-3', 5'-AGGTTGTTACTCGTTCGGATCCT-3') was used as an endogenous control and an amplicon corresponding to the M. truncatula phosphate transporter 4 (AY116210; Harrison et al., 2002; 5'-ACAAATTTGATAGGATTCTTTTGCACGT-3', 5'-TCACATCTTCTCAGTTCTTGAGTC-3') as a positive control. c_t-Values were calculated by substracting the c_t-value for the constitutively expressed translation elongation factor 1 from the c_t-values of the various samples. cDNA assays without reverse transcriptase (RT^–) were used as a control for a possible amplification of contaminating DNA material. We only evaluated samples when these real-time PCR assays either gave no amplification or when the weak signals eventually observed accounted for <2% of the template measured in nonmycorrhizal roots.

Analysis of Nonpolar Metabolite Steady-State Levels

Roots of M. truncatula were harvested 40 d after inoculation with G. intraradices. After addition of 15 µL methyl nonadecanoate (0.2 mg mL^–1 CHCl₃, internal standard), lyophilized roots (30 mg) were homogenized in a mortar with addition of solid CO₂ and extracted three times with 0.5 mL hexane. Aliquots of the extracts were derivatized using N-methyl-N-(trimethylsilyl)-trifluoroacetamide (30 min, 70°C) after reducing to dryness. Metabolites were separated and analyzed using a gas chromatograph (Agilent 6890) equipped with a TOF-MS (GCT; Waters). Column specifications are as follows: DB-5 MS 30 m x 0.25 mm, i.d.; 0.25-µm film thickness; carrier gas, helium at constant flow 1 mL min^–1; temperature program, 2 min 50°C, then 10°/min to 300°, 10 min 300°, 10°/min to 320°, 11 min at 320°. Splitless injection was as follows: 1 µL at 250°; mass-to-charge ratio 40 to 800.

Analysis of Polar Metabolite Steady-State Levels

Metabolites were extracted according to Roessner et al. (2000) with the following modifications. Aliquots of ground root samples were mixed with 360 µL methanol (–20°C) plus 30 µL ribitol in methanol (0.2 mg mL^–1) and 30 µL d₄-Ala in water (1 mg mL^–1). Samples were shaken for 15 min at 70°C before addition of 200 µL chloroform and further shaking at 37°C for 5 min. After addition of 400 µL water, samples were vortexed, then centrifuged at 21,000g for 5 min at room temperature. Two 80-µL aliquots of the aqueous phase were transferred to Eppendorf tubes and dried at room temperature by vacuum centrifugation (Centrivac; Heraeus). Metabolite derivatization was performed according to Roessner et al. (2000) with the following modifications. One of each pair of dried samples was resuspended in 40 µL methoxyamine hydrochloride (20 mg mL^–1 in pyridine) at 30°C for 90 min and 70 µL N-methyl-N-(trimethylsilyl)-trifluoroacetamide plus 10 µL alkane mixture (see below). GC-MS spectra were obtained with a GC8000 gas chromatograph coupled to a Voyager quadrupol-type mass spectrometer operated by MassLab software (ThermoQuest). Modifications to the initial GC-MS profiling method (Fiehn et al., 2000a, 2000b) included injection of a 1-µL sample in splitless mode, use of a 5°C min^–1 temperature ramp with final temperature set to 320°C on a 30 m x 0.25 mm i.d. Rtx-5Sil MS capillary column with an integrated guard column (Restek), and use of the C₁₂, C₁₅, C₁₉, C₂₂, C₃₂, and C₃₆ n-alkane mixtures for the determination of retention time index. Metabolites were identified and quantified as detailed by Colebatch et al. (2004).

	ACKNOWLEDGMENTS

 
The authors thank Dr. J.P. Marques (Martin-Luther-University Halle-Wittenberg, Germany) and Dr. R.B. Klösgen (Martin-Luther-University Halle-Wittenberg, Germany) for kindly providing a transformation construct for targeting the GFP into plastids, Dr. Y. Niwa (University of Shizuoka, Japan) for a similar construct regarding the labeling of mitochondria, and Dr. P.A. Olsson (University of Lund, Sweden) for cooperation in identifying fatty acids.

Received February 16, 2005; returned for revision June 2, 2005; accepted June 7, 2005.

	FOOTNOTES

 
¹ This work was supported by the Deutsche Forschungsgemeinschaft (Bonn, Germany).

^[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.061457.

^* Corresponding author; e-mail tfester{at}ipb-halle.de; fax 49–345–5582–1509.

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