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First published online December 16, 2005; 10.1104/pp.105.072967 Plant Physiology 140:140-149 (2006) © 2006 American Society of Plant Biologists The Arabidopsis Group 1 LATE EMBRYOGENESIS ABUNDANT Protein ATEM6 Is Required for Normal Seed Development1,[W]Department of Genetics and Biochemistry (A.J.M., L.M.L., W.R.M.) and Department of Biological Sciences (W.R.M.), Clemson University, Clemson, South Carolina 29634–0324
As part of the embryo maturation process, orthodox seeds undergo a developmentally regulated dehydration period. The LATE EMBRYOGENESIS ABUNDANT (LEA) genes encode a large and diverse family of proteins expressed during this time. Many hypothesize that LEA proteins act by mitigating water loss and maintaining cellular stability within the desiccated seed, although the mechanisms of their actions remain largely unknown. The model plant Arabidopsis (Arabidopsis thaliana) contains two genes belonging to the group 1 LEA family, ATEM1 and ATEM6, and knockout mutations in these genes are being sought as a means to better understand group 1 LEA protein function during embryo maturation. We have identified a T-DNA insertion allele of the ATEM6 gene in which the T-DNA is present just downstream of the protein coding region. While this gene is transcriptionally active and encodes a wild-type protein, there is no detectable ATEM6 protein in mature seeds. Mutant seeds display premature seed dehydration and maturation at the distal end of siliques, demonstrating that this protein is required for normal seed development. We propose that one function for group 1 LEA proteins in seed development is to buffer the water loss that occurs during embryo maturation and that loss of ATEM6 expression results in the mutant phenotype.
Seeds are remarkable structures that serve as the connection between successive generations of plants. As part of the normal developmental program of orthodox seeds, most cellular water is lost during the latter stages of embryo maturation and many seeds dehydrate to as low as 5% of their original water content. Despite the low level of retained water, seed embryos remain viable and mature, and dry seeds readily germinate under appropriate environmental conditions. Therefore, plants must have evolved a mechanism that allows cells to tolerate the low water potential found in dry seeds.
The LATE EMBRYOGENESIS ABUNDANT (LEA) genes are expressed, as the name implies, late in embryo maturation and during the developmentally regulated period of dehydration at the end of seed development. LEA proteins are thought to play an important role in the maturation process, and studies in barley (Hordeum vulgare) have demonstrated that accumulation of LEA proteins in embryos is correlated with the acquisition of desiccation tolerance (Bartels et al., 1988
LEA genes are found throughout higher plants, including monocots, dicots, and gymnosperms, and differences in temporal expression pattern and sequence similarity among the encoded proteins have allowed them to be subdivided into groups (Dure et al., 1989
The group 1 LEA genes, also called the Em (Thompson and Lane, 1980
There are two group 1 LEA genes, ATEM1 and ATEM6, in the genome of the model plant Arabidopsis (Arabidopsis thaliana), and these genes are differentially expressed both temporally and spatially during embryo maturation. ATEM1 mRNA is expressed predominantly in provascular tissues with the strongest expression in the root tip (Vicient et al., 2000
To study the in planta function of the ATEM proteins during embryo maturation, a population of more than 100,000 T-DNA-mutagenized Arabidopsis lines maintained by Syngenta (Sessions et al., 2002
Approximately 3 kb of Arabidopsis genomic DNA sequence containing the ATEM6 locus (At2g40170) was provided to the Torrey Mesa Research Institute for a search of the Syngenta T-DNA insertional mutation database (Sessions et al., 2002  ). The results of the BLAST search were analyzed and the sites of two potential insertions within this gene were identified (Table I). One plant line, Garlic_413_E08, contains T-DNA from pCSA110 (McElver et al., 2001) and was predicted to contain an insertion at position 16,786,882 on chromosome 2. This location is within exon 2 of the ATEM6 coding region and 10 bp 5' of the TAA translational stop codon. The insertion in the other line, Garlic_587_G09, contains T-DNA from pCSA104 (McElver et al., 2001) and was predicted to be located at position 16,787,092 on chromosome 2. This location is within the single intron of ATEM6.
Seeds for the two plant lines were obtained, grown in soil, and both seed and vegetative tissue were harvested from the initial planting. Genomic DNA was extracted from the bulked plant tissue of each line and subjected to Southern-blot analysis using T-DNA-specific (pBluescript [pBS]) and ATEM6 gene-specific (3'-untranslated region [UTR]) probes after digestion with an enzyme that cleaves once near the center of the T-DNA. While both lines displayed multiple bands when hybridized with the T-DNA probe (pBS), indicating the presence of multiple insertion sites, only the Garlic_413_E08 line displayed a band other than that found in wild-type Columbia genomic DNA when hybridized with the ATEM6 gene-specific 3'-UTR probe (data not shown). This demonstrates that the Garlic_413_E08 line contains a T-DNA in or near ATEM6 and that the Garlic_587_G09 line does not. This Garlic_413_E08 plant line will hereafter be referred to as 6.413, as a reference to the ATEM6 gene and the Garlic plant line number supplied by Syngenta.
Southern hybridization assays using an ATEM6 3'-UTR gene-specific probe were performed to identify individual plants containing the T-DNA insertion identified above. Digestion of genomic DNA with BamHI should produce an approximately 11.4-kb DNA fragment containing both the pBS portion of the T-DNA and the ATEM6 3'-UTR region for the T-DNA insertion allele (Fig. 1A ), whereas the wild-type ATEM6 allele should produce an 8,470-bp fragment, a difference of approximately 3.0 kb. Figure 2 shows that plants grown from the original bulked seed are segregating for the insertion and that individual plants homozygous for the wild-type ATEM6 allele (e.g. lines 1.2, 2.2, and 6.4), homozygous for the T-DNA insertion allele (e.g. lines 2.1, 3.2, and 8.2), and heterozygotes (e.g. line 7.2) are clearly distinguishable. Hybridization of the same membranes with a pBS probe demonstrated that all individuals contained multiple T-DNA inserts (data not shown). Plants homozygous for the ATEM6 insertion allele were backcrossed into wild-type Columbia and the resulting seedlings screened using the Southern-blot analysis described above to identify plants with only the ATEM6-associated T-DNA. These heterozygous plant lines were allowed to self and plant lines homozygous for the insertion allele were subsequently identified.
Plasmid Rescue
If the T-DNA present in the 6.413 plant line represents a simple, single insertion, as diagrammed in Figure 1A, the insert should be bounded by the left border (LB) and right border (RB) regions and it should be possible to PCR amplify boundary fragments using LB or RB and ATEM6 gene-specific primers. However, initial attempts at PCR failed to produce any products, suggesting that the DNA at the site of the insertions may have undergone some rearrangement, a relatively frequent observation for T-DNA insertions (Castle et al., 1993 The EcoRI experiment to define RB junctions with genomic DNA rescued two plasmids (6.413-1E and 6.413-2E). When plasmid DNA from the two rescued colonies was extracted and run on an agarose gel for size determination, the two linearized plasmids appeared to be approximately the same size (approximately 7,500 bp). However, digestion with NcoI resulted in different banding patterns, demonstrating that these two plasmids are unique (data not shown). Sequence analysis of plasmid 6.413-1E indicated that this T-DNA insert was not within the ATEM6 gene locus, but rather at position 16,474,005 on chromosome 1 in the 5'-LB to RB-3' orientation (with respect to the 5' to 3' orientation of the chromosome sequence; Table II). This insertion is most closely linked to locus At3g11860 that encodes a hypothetical protein. In addition, our data show that 189 bp of the RB sequence have been deleted. Sequence analysis of plasmid 6.413-2E confirmed the insertion of a T-DNA in a 5'-RB to LB-3' orientation (with respect to the 5' to 3' orientation of the chromosome sequence) with its RB/ATEM6 junction at position 16,786,867 on chromosome 2 (Fig. 1B; Table II). The sequence analysis further revealed a 767-bp deletion at the RB of the T-DNA immediately 5' to the insert location.
The NcoI rescue experiment yielded nine plasmids (6.413-1N to 6.413-9N). Sequence analysis of one of these plasmids, 6.413-2N, indicated that this T-DNA insert was not within the ATEM6 gene locus, but rather at position 19,134,716 on chromosome 3 in the 5'-LB to RB-3' orientation (with respect to the 5' to 3' orientation of the chromosome sequence; Table II). This insertion is most closely linked to locus At3g51560 that encodes a disease resistance protein of the Toll interleukin receptor-nucleotide-binding site-Leu-rich repeat class. The remaining eight plasmids, 6.413-1N and 6.413-3N to 6.413-9N, defined the ATEM6/LB junction at position 16,786,868 on chromosome 2 (Fig. 1B; Table II). Sequence data further indicated a deletion of 267 bp in the T-DNA LB region directly adjacent to the insert location. Therefore, this T-DNA insertion is located 15 bp 3' of the predicted insertion site as determined by the initial BLAST search or 2 bp downstream of the TAA stop codon in ATEM6 (i.e. outside the ATEM6 open reading frame). Whereas there is deletion of T-DNA sequences at both ends of the insert, there is no accompanying deletion of ATEM6 sequences. As such, the ATEM6 open reading frame in exon 2 remains intact and still encodes a normal protein. The deletions in both the T-DNA LB and RB regions explain our earlier inability to PCR amplify products from these locations using Syngenta primers in conjunction with ATEM6 gene-specific primers. Furthermore, this suggests that the deletions occurred after the sequence information identified by BLAST search was generated for the Syngenta database (Sessions et al., 2002 ). Hereafter, the plant lines containing the T-DNA insertion in the ATEM6 locus will be referred to as atem6-1. Two additional inserts identified in the Garlic_587_G09 line, including nearest gene and putative function, are also shown in Table II.
The plasmid rescue results described above indicated that there were deletions in both borders of the atem6-1 T-DNA. Although sequence analysis of the regions directly adjacent to the insertion did not indicate any chromosomal alterations, it remained possible that other aberrations on chromosome 2 had occurred. Therefore, one simple sequence-length polymorphism (NGA168) and two cleaved-amplified polymorphic sequence (Thy1 and ML) markers were chosen to map the large arm of chromosome 2. Thy1 is located close to the centromere, NGA168 is tightly linked to ATEM6, and ML is located close to the telomere. All markers mapped to chromosome 2 and in the correct order as compared to ATEM6 and to each other, indicating that there were no gross chromosomal abnormalities associated with the atem6-1 insertion (Fig. 3 ).
Reverse Transcription-PCR and RNA Gel-Blot Analysis of Seeds Sequence analysis from the plasmid rescue experiments demonstrated that the atem6-1 T-DNA insertion occurred 2 bp outside of the ATEM6 open reading frame. Since both the promoter and coding regions of the mutant allele remained intact, it could be possible for this gene to express an mRNA that encodes a wild-type protein. It was of interest, therefore, to determine whether this mutant allele expresses an mRNA. Total RNA extracted from mature wild-type seeds and from mature seeds homozygous for the atem6-1 allele was subjected to reverse transcription (RT)-PCR. The RT reaction was performed using an oligo(dT)17 primer containing a V clamp at the 3' end and a 20-bp 5' extension (Table III). Subsequent PCR was performed using primers designed to the ATEM6 first and second exons such that a 198-bp product should be amplified from ATEM6-derived mRNA for both the wild-type and atem6-1 mutant allele. Wild-type Columbia DNA was subjected to PCR in a separate reaction as a control for contaminating genomic DNA. Because the PCR primers flank the single intron in ATEM6, a product derived from a DNA template should be 303 bp. The generation of a 198-bp product from both wild-type atem6-1 mutant seeds demonstrates that an ATEM6-derived mRNA is actively transcribed in the mutant (Fig. 4 ).
RNA gel-blot analysis of total RNA samples was then used to determine the level of expression and size of the wild-type and mutant transcripts. Previous cDNA characterization has shown that wild-type ATEM6 transcripts are approximately 540 and 640 nucleotides in length prior to polyadenylation (Gaubier et al., 1993 ). Because of the location of the T-DNA insertion in the mutant, the 3'-UTR region is physically separated from the remainder of the ATEM6 gene. Therefore, membranes were hybridized using an ATEM6 5'-UTR probe. As shown in Figure 5
, the ATEM6 message is readily detected in wild-type mature seed RNA and is of a size consistent with the earlier study. Seeds homozygous for the mutant atem6-1 allele, however, contain significantly lower levels of a slightly smaller transcript, approximately 12% of that found in wild type.
To determine the 3' end of the mutant mRNA and to ensure that the seed from which the RNA was extracted was not contaminated with wild type, the RT reaction of atem6-1 mature seed RNA from above was used for 3'-RACE. The 5' exon 1 RT-PCR primer was used in combination with a 3' primer representing the 5' extension on the RT primer (Table III). The products were cloned as blunt-ended fragments into pBS after treatment with Klenow fragment and T4 DNA kinase. Sequence analysis of eight clones identified two classes of transcripts from the atem6-1 mutant allele that are polyadenylated at 103 and 114 nucleotides after entering the T-DNA insert. None of the clones contained any wild-type ATEM6 3'-UTR sequences except the 2 bp that separate the T-DNA insertion from the open reading frame.
Since the mutant atem6-1 seeds express a mature ATEM6-derived mRNA, albeit at a significantly lower level than that found in wild type, it was of interest to determine whether these seeds also translate that mRNA into ATEM6 protein. Protein was isolated from mature seeds of a single silique from wild-type and homozygous atem6-1 plants as well as from a silique of a backcross of a homozygous atem6-1 mutant plant to wild type (i.e. heterozygous seed). The extracts were separated on a 15% Tris-Gly gel, transferred to a polyvinylidene difluoride (PVDF) membrane, and ATEM proteins were detected using a polyclonal rabbit anti-wheat Em protein antibody. Figure 6 shows that this antibody detects both ATEM6 and ATEM1 proteins in wild-type seed extracts. ATEM6 protein is also readily detected in the heterozygous atem6-1 seed extracts, but is essentially undetectable in homozygous atem6-1 mutant seed extracts. Therefore, although there is an actively transcribed message present in mature homozygous mutant atem6-1 seeds, there is no detectable ATEM6 protein.
Phenotypic Analysis of atem6-1 Mutant Plants
ATEM6 mRNA and ATEM6 protein are normally expressed only in embryonic tissues and only during the final stages of seed maturation (Gaubier et al., 1993 Under the growth conditions used in this study, silique and seed development was complete with mature dry seeds being present 16 to 17 d after flowering (daf). For this study, the phenotype of siliques ranging in age from 7 through 17 daf was examined. When homozygous atem6-1 mutant plants and wild-type Columbia plants were grown in parallel, it was observed that seed and silique development is also indistinguishable through about 12 daf. After that period, however, there is a visible difference between mutant and wild type and, at 13 and 14 daf, seeds at the distal end of siliques of mutant plants begin to dehydrate prematurely (Fig. 7, A and B ). In addition, the distal ends of the mutant siliques also dehydrate early and, in the most severe cases, split open prior to any dehydration of seeds or siliques at the proximal ends.
To verify that earlier stages of seed development are not affected in this mutant, seeds were dissected from siliques at 8 to 15 daf, cleared using Hoyer's solution (Liu and Meinke, 1998 ), and visualized by Nomarski optics. There was no obvious difference between mutant and wild-type seed development through 12 daf (data not shown). When the phenotype was visible (13–14 daf), embryos at the base of the silique were visually as developed as those at the tip, the only difference being the premature dehydration of seeds at the distal end. Therefore, development of mutant embryos appears to be synchronous along the length of the silique, whereas the onset of dehydration is not. Consistent with expression only in embryonic tissues and only during the maturation phase of embryo development, growth at all other stages and in all other tissues appears indistinguishable from wild type (data not shown).
The wild-type ATEM6 allele was reintroduced into homozygous atem6-1 mutant plants by direct T-DNA transformation using a 3,002-bp genomic clone. PCR was used to verify that the plants contained both the atem6-1 mutant allele and the ATEM6 complementation construct. Phenotypic characterization (as described above) indicated that the presence of the ATEM6 complementation construct is sufficient to rescue the phenotype of the atem6-1 mutant allele (Fig. 7C). Whereas mutant siliques exhibited premature desiccation of the seeds at the tip, wild-type and the atem6-1-complemented seeds desiccated synchronously along the length of the silique.
During normal development, the Arabidopsis genes ATEM1 and ATEM6 are expressed only in embryos. The temporal expression pattern of these two genes is slightly different, with ATEM1 induction preceding ATEM6 by about 2 d. Spatial expression patterns also differ with ATEM1 expressed predominantly in provascular tissue and the root meristem, whereas ATEM6 is expressed throughout the embryo with greater expression in provascular tissue and the shoot meristem (Vicient et al., 2000 ). Therefore, the proteins encoded by these genes are not functionally redundant in seed maturation. By BLAST search of a T-DNA insertional mutant population, we identified a putative mutation in the ATEM6 gene. Molecular characterization of the insertion revealed that the T-DNA had inserted just downstream of the TAA stop codon for the open reading frame. Insertion at this location results in retention of an intact 5' flanking region and an unaltered protein coding capability. Barring the involvement of downstream regulatory elements, one would predict that this insertion allele should exhibit normal transcriptional activity. Indeed, our results from RT-PCR demonstrate that this gene is transcribed and spliced in maturing seeds and that the transcripts are polyadenylated, at least to some extent. As evidenced by RNA gel-blot analysis, however, the transcript levels appear significantly reduced in dry seed. The lower level of mRNA expression in the mutant could be explained in several ways. First, the onset of expression could be delayed or the rate of transcription could be lower. Although we think this is unlikely, we are currently investigating temporal regulation of ATEM6 in the atem6-1 mutant to determine whether it is comparable to that of wild-type plants. An alternative explanation, and the one we favor, is that abnormal transcription termination and/or polyadenylation of the mRNA is responsible for the decrease in mature seed mRNA levels. Our 3'-RACE data show that transcripts from the atem6-1 insertion allele are polyadenylated, at least to some extent, and terminate at two different places in the T-DNA. This suggests the use of cryptic or fortuitous termination signals present in the T-DNA sequences resulting in the production of a chimeric RNA that may have an altered stability with respect to wild type. In any case, the normal ATEM6 termination and polyadenylation signals will not be present as the T-DNA insert physically separates the normal 3' region for this gene from the remainder by over 7.5 kb. The likelihood that a conventional termination/polyadenylation process is functioning, particularly one associated with a gene that is normally expressed to high levels, is remote.
Our data further demonstrate that, although the T-DNA insert is located outside the ATEM6 coding region of the gene, there is little or no ATEM6 protein present in mature seeds. This is not due to an inability to detect ATEM proteins in extracts from cells that contain the insertion allele because both ATEM6 and ATEM1 proteins are seen in extracts from heterozygotes. While the antibody used is polyclonal, it is likely that similar or identical epitopes are recognized for both proteins based on the high level of amino acid sequence identity. In addition, ATEM1 protein is readily detected in extracts from seeds homozygous for the atem6-1 allele. Because the coding region of the gene is unchanged and the mRNA is appropriately spliced, protein translated from that mRNA should display all the characteristics of wild-type protein, including stability. We hypothesize, therefore, that the decrease in protein levels is due, at least in part, to altered stability of the chimeric mRNA. This is supported by the presence of significantly lower levels of the ATEM6 transcript in the homozygous mutant. In addition, if polyadenylation is poor or inefficient, there could be a significant effect on the process of ribosome recycling (Munroe and Jacobson, 1990 The severe reduction or elimination of ATEM6 protein production is manifested in homozygous mutant plants as a distinct phenotype during late seed maturation. At a time in development when seeds from wild-type plants are still fully green and hydrated along the length of the silique, mutant seeds at the distal end of siliques display premature drying. In addition, other aspects of the maturation process appear to be accelerated as evidenced by the accumulation of pigments in those same seeds. Display of the phenotype associated with elimination of this single group 1 LEA protein corresponds to the normal developmental time for the onset of ATEM6 expression, and beyond 14 daf the remaining mutant seeds mature rapidly and relatively synchronously. This phenotype is not present in plants heterozygous for the atem6-1 mutation, suggesting that the level of ATEM6 protein produced from a single wild-type allele is sufficient for normal seed development. Complementation of the atem6-1 mutation using a 3,002-bp ATEM6 allele restored the wild-type phenotype of the homozygous mutant seed. This demonstrates that the presence of ATEM6 is necessary to maintain hydration throughout the length of the silique during the later stages of seed development. Premature desiccation was not observed in all mutant siliques and occasionally there was early drying of a few wild-type siliques. However, the frequency of premature drying in atem6-1 plants was significantly higher and much more severe than the number observed in wild-type plants (Supplemental Table I). This suggests that the phenotype associated with the atem6-1 mutation is variably expressed. Statistical analysis (LSD-ANOVA) was used to determine whether the phenotype of a population of wild-type plants was statistically different from that of a population of atem6-1 mutants and demonstrated that the phenotypes of the two populations are not the same (P value < 0.001; Supplemental Table II). LSD-ANOVA analysis was also used to compare the phenotypes of wild-type, atem6-1 mutant, and atem6-1 complement populations. The results indicated that the phenotype of the complementation plants is not different from the wild type (P value < 0.001) and that the atem6-1 mutant phenotype is different from both the wild-type and complement plants (P value < 0.001; Supplemental Table II.). A three-way contingency table was used to determine whether any of the silique phenotypes could be due to chance or whether they were based on plant type (wild type, mutant, or complement). The results indicated that the phenotypes could only be due to plant type (P = 0.001; Supplemental Table III).
Several studies have demonstrated that rearrangements, deletions, and inversions may be a relatively frequent occurrence at sites of T-DNA integration (Castle and Meinke, 1994 Based on our observations, ATEM6 protein expression is necessary for normal seed development. While characterization of the atem6-1 mutant plants at this level does not allow us to directly address some of the functions proposed for group 1 LEA proteins (e.g. the water replacement hypothesis), the phenotype suggests that expression of ATEM6 protein may be required to buffer the rate of dehydration during the latter stages of seed maturation. This is consistent with the very hydrophilic nature of group 1 LEA proteins, yet does not preclude involvement in other mechanisms. Our hypothesis is also consistent with initial display of the phenotype in seeds that are most distal from the remainder of the plant because those would be the first to experience lower water potentials. Interestingly, this mutation is not lethal and mutant seeds are indistinguishable from wild type visually and readily germinate under laboratory conditions. We are currently exploring the possibility that expression of the other group 1 LEA, ATEM1, displays an altered level or domain of expression in mutant seeds that might in some way compensate for loss of ATEM6 expression. An alternative, although not mutually exclusive, explanation is that the near-optimal conditions used for plant propagation in the laboratory setting do not reveal a decrease in fitness that might be apparent in mutant plants grown in the wild. The exact mechanisms by which the group 1 LEA proteins contribute to dehydration/desiccation tolerance remain to be fully elucidated.
Plant Materials and Growth Conditions
The wild-type Arabidopsis (Arabidopsis thaliana) plants in this work are of the Columbia-0 ecotype. The putative T-DNA insertions in ATEM6 were identified in the Syngenta Arabidopsis Insertion Library (Sessions et al., 2002
Leaf material was collected, ground under liquid nitrogen, and chromosomal DNA was extracted as described previously (Krysan et al., 1996
Homozygous atem6-1 plants (Columbia ecotype) were crossed to wild-type Landsberg erecta. Heterozygous F1 plants were allowed to self-pollinate, generating a segregating F2 population. F2 plants were grown on soil and leaf tissue was collected from 100 individuals for genomic DNA extraction. Leaves were removed and immediately minced in a microcentrifuge tube with a plastic pestle. Each sample was vortexed with 200 µL of extraction buffer (100 mM Tris-HCl, pH 9.5; 1 M KCl; 10 mM EDTA) and incubated at 65°C for 30 min. Samples were centrifuged at 13,000 rpm at room temperature for 10 min. The supernatant was precipitated with 500 µL 100% ethanol, placed at –20°C for 30 min, and then centrifuged at 13,000 rpm at room temperature for 15 min. DNA pellets were washed with 70% ethanol, centrifuged at 13,000 rpm for 2 min at room temperature, and resuspended in 10 mM Tris HCl, pH 8.0, 0.1 mM EDTA (Tris-EDTA). All PCR reactions were run using the following components: 10 mM Tris-HCl, pH 8.3, 50 mM KCl, 2 mM MgCl2, 0.2 mM dNTPs, 12 pmol each primer (see below), and 2 units of Taq polymerase (all primer sequences are listed in Table III). The NGA168 marker (NGA168-F and NGA168-R primers) was amplified with the following program: 94°C, 5 min for one cycle; 94°C, 1 min, 49°C, 1 min, 72°C, 1 min for 10 cycles; 94°C, 1 min, 51°C, 1 min, 72°C, 1 min for 15 cycles; and 72°C, 7 min. The ML and Thy1 markers, the ATEM6 allele, and the atem6-1 allele were amplified using the following program with varying annealing temperatures (Ta): 94°C, 5 min for one cycle; 94°C, 1 min, Ta, 1 min, 72°C, 1 min for 35 cycles; and 72°C, 7 min. The ML-F and ML-R primers used a Ta of 51°C, the Thy1-F and Thy1-R primers used a Ta of 50°C, the ATEM6 allele (ATEM6 gene-specific 2 and ATEM6 gene-specific 3 primers) used a Ta of 55°C, and the atem6-1 allele (ATEM6 gene-specific 2 and AJM-LB1 primers) used a Ta of 50°C. All PCR products were size fractionated on a 0.7% agarose gel.
Plasmid and genomic DNAs were subjected to restriction endonuclease digestion and size fractionated on 0.7% agarose gels at 15 to 40 V overnight. The DNA was then blotted onto a Hybond N+ membrane using a downward alkaline transfer (Chomczynski, 1992
Ten seeds were pulverized at room temperature in a sterile conical microcentrifuge tube with a plastic pestle cleaned with 95% ethanol. RNA was extracted from the seeds according to the protocol described (Chomczynski and Sacchi, 1987
RNA was extracted as described (Vicient and Delseny, 1999
Protein was extracted from a single silique minced in a microcentrifuge tube containing 200 µL of an extraction buffer containing 10 mM Tris-HCl, pH 8.3, and 10 mM NaCl. The tubes were vortexed well, then centrifuged for 2 min at 10K rpm at 4°C. The supernatant was removed from the seed tissue and put into a new microcentrifuge tube and centrifuged two or three times until no solid material remained at the bottom of the tube. Extracts were then frozen at –20°C and put directly into the centrifuge at maximum speed at 4°C for 30 min. The supernatant was transferred to a new tube and heated to 80°C for 20 min followed by centrifugation at maximum speed at 4°C for 30 min. This supernatant was transferred to a fresh tube and precipitated with 25% TCA. Samples were incubated on ice for 5 min, then centrifuged at maximum speed at 4°C for 5 min. The supernatant was removed and the protein pellets were washed with 100% acetone and centrifuged for another 5 min at 4°C. The acetone was removed, the pellets dried slightly, and resuspended directly in gel-loading buffer. Protein extracts were resolved on a 15% Tris-Gly gel and electrotransferred to an Immobilon-P PVDF membrane (Millipore) at 100 V for 1 h at 4°C. Blots were processed using a primary polyclonal rabbit anti-wheat Em antibody, a secondary alkaline phosphatase-conjugated goat anti-rabbit antibody. Chemiluminescent detection was performed using ready-to-use CSPD (Roche Diagnostics).
A 3,002-bp ATEM6 genomic clone was PCR amplified using the ATEM6-F and ATEM6-R primers (Table III) and cloned into the SmaI site of pBS (Stratagene). The genomic clone and pBI101 vector were digested using BamHI and EcoRI, run on a 0.7% agarose gel, and the desired fragments isolated. Insert and vector were ligated and transformed into E. coli strain DH5
Pots containing 10 to 20 homozygous atem6-1 mutant plants were grown until the first inflorescence meristems reached a length of at least 10 cm. Agrobacterium cultures were grown as described (Clough and Bent, 1998
Seeds were surface sterilized with 70% ethanol for 1 min, 10% bleach, containing 0.1% Triton X-100 for 10 min and rinsed with sterile distilled water three times. Germination media consisted of 0.5x Murashige and Skoog media (one packet of Murashige and Skoog minimal salts [no. M6899; Sigma] supplemented with 1% Suc, 0.8 mg/L thiamine, 0.5 mg/mL pyridoxine, 0.5 mg/mL nicotinic acid, and 50 mg/L myoinositol) and 0.8% Phytagar was supplemented with 50 µg/mL kanamycin sulfate and 125 µg/mL carbenicillin. Sterile seeds were mixed with 0.5x Murashige and Skoog Phytagar germination media and poured onto selection plates. To prevent drying or contamination while allowing gas exchange, plates were wrapped with latex-free medical bandaging tape. Plates were cold treated at 4°C for 3 d, then moved into the growth chamber. Seeds remained on the selection media for 7 d until green seedlings with first true leaves were identified. These seedlings were placed on 0.5x Murashige and Skoog nonselective media for 7 d, then moved onto soil. PCR was used to confirm that the selected plants contained both the atem6-1 allele and the wild-type ATEM6 complement. All PCRs were done using 10 mM Tris-HCl, pH 8.3, 50 mM KCl, 2 mM MgCl2, 0.2 mM dNTPs, 12 pmol each primer (see below), and 2 units of Taq polymerase. The following program (with variable Ta) was used for all PCRs: 94°C, 5 min for one cycle; 94°C, 1 min, Ta, 1 min, 72°C, 1 min for 30 cycles; and 72°C, 7 min. The ATEM6 gene-specific 2 and AJM LB-1 primers (Ta = 50°C) were used to amplify the atem6-1 allele. The ATEM6 gene-specific 2 and ATEM6 gene-specific 3 primers (Ta = 55°C) were used to amplify the wild-type ATEM6 allele. Primers specific to the kanamycin NptII gene (Ta = 50°C) present within the pBI101 T-DNA were also used to verify the presence of the complementation T-DNA. All primer sequences are listed in Table III.
The phenotypes of seeds within the silique were determined by dissecting the silique from base or pedicel to tip using Dumont number 4 and 5 forceps. The silique was held in place at the pedicel while the forceps were used to dissect longitudinally along the natural line of dehiscence. One-half of each valve covering the seeds was removed to expose the seeds while keeping them in situ. Images of the seeds were taken with a Nikon SMZ1500 dissecting scope using Q-Capture software (version 2.68.6; Q-Imaging). A score of wild type was assigned if the seeds were desiccating synchronously along the length of the silique. A score of mutant was assigned if the first one to five seeds in the silique were mature and desiccated, while the remaining seeds were green. The numerical values from the phenotype study (percent wild type-looking siliques) were converted to a normal distribution using an arcsine-squared conversion. Statistical analysis of phenotype was done using Systat software (version 10; Systat).
We thank Julie M. Long for technical assistance and Julia Frugoli for critical reading of the manuscript. We also thank Torrey Mesa Research Institute/Syngenta for providing the putative Arabidopsis T-DNA insertion lines. Received October 17, 2005; returned for revision October 17, 2005; accepted November 1, 2005.
1 This work was supported by the National Research Initiative of the U.S. Department of Agriculture (USDA) Cooperative State Research, Education and Extension Service (CSREES; grant no. 2002–35318–12627 to W.R.M.); the CSREES/USDA (project no. SC–1700200 to W.R.M.); the Howard Hughes Medical Institute SC-LIFE Undergraduate Research Program; and the Clemson University Calhoun Honors College, Technical Contribution Number 5061 of the Clemson University Experiment Station.  The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: William R. Marcotte Jr. (marcotw{at}clemson.edu).
[W] The online version of this article contains Web-only data. Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.072967. * Corresponding author; e-mail marcotw{at}clemson.edu; fax 864–656–0119.
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ABSTRACT
RESULTS
MCR, using ampicillin at 100 µg/mL for selection. Plasmid DNA was sequenced using the Big Dye version 3.0 kit according to the manufacturer's instructions (Applied Biosystems). Plasmids rescued using EcoRI digestion were sequenced using a T7 promoter primer and the QRB2 primer (
