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Update on T-DNA Binary Vectors

T-DNA Binary Vectors and Systems

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907–1392

For more than two decades, scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants. Initial technologies to introduce genes of interest (goi) into Agrobacterium involved complex microbial genetic methodologies that inserted these goi into the transfer DNA (T-DNA) region of large tumor-inducing plasmids (Ti-plasmids). However, scientists eventually learned that T-DNA transfer could still be effected if the T-DNA region and the virulence (vir) genes required for T-DNA processing and transfer were split into two replicons. This binary system permitted facile manipulation of Agrobacterium and opened up the field of plant genetic engineering to numerous laboratories. In this review, we recount the history of development of T-DNA binary vector systems, and we describe important components of these systems. Some of these considerations were previously described in a review by Hellens et al. (2000b).

Agrobacterium transfers T-DNA, which makes up a small (approximately 5%–10%) region of a resident Ti-plasmid or root-inducing plasmid (Ri-plasmid), to numerous species of plants (DeCleene and DeLey, 1976; Anderson and Moore, 1979), although the bacterium can be manipulated in the laboratory to transfer T-DNA to fungal (Bundock et al., 1995; Piers et al., 1996; de Groot et al., 1998; Abuodeh et al., 2000; Kelly and Kado, 2002; Li et al., 2007) and even animal cells (Kunik et al., 2001; Bulgakov et al., 2006). Transfer requires three major elements: (1) T-DNA border repeat sequences (25 bp) that flank the T-DNA in direct orientation and delineate the region that will be processed from the Ti/Ri-plasmid (Yadav et al., 1982); (2) vir genes located on the Ti/Ri-plasmid; and (3) various genes (chromosomal virulence [chv] and other genes) located on the bacterial chromosomes. These chromosomal genes generally are involved in bacterial exopolysaccharide synthesis, maturation, and secretion (e.g. Douglas et al., 1985; Cangelosi et al., 1987, 1989; Robertson et al., 1988; Matthysse, 1995; O'Connell and Handelsman, 1999). However, some chromosomal genes important for virulence likely mediate the bacterial response to the environment (Xu and Pan, 2000; Saenkham et al., 2007). Several recent reviews enumerate factors involved in and influencing Agrobacterium-mediated transformation (Gelvin, 2003; McCullen and Binns, 2006).

The vir region consists of approximately 10 operons (depending upon the Ti- or Ri-plasmid) that serve four major functions.

(1) Sensing plant phenolic compounds and transducing this signal to induce expression of vir genes (virA and virG). VirA and VirG compose a two-component system that responds to particular phenolic compounds produced by wounded plant cells (Stachel et al., 1986). Because wounding is important for efficient plant transformation, Agrobacterium can sense a wounded potential host by perceiving these phenolic compounds. Activation of VirA by these phenolic inducers initiates a phospho-relay, ultimately resulting in phosphorylation and activation of the VirG protein (Winans, 1991). Activated VirG binds to the vir box sequences preceding each vir gene operon, allowing increased expression of each of these operons (Pazour and Das, 1990). In addition to induction of the vir genes by phenolics, many sugars serve as co-inducers. These sugars are perceived by a protein, ChvE, encoded by a gene on the Agrobacterium chromosome. In the presence of these sugars, vir genes are more fully induced at lower phenolic concentrations (Peng et al., 1998).

(2) Processing T-DNA from the parental Ti- or Ri-plasmid (virD1 and virD2). Together, VirD1 (a helicase) and VirD2 (an endonuclease) bind to and nick DNA at 25-bp directly repeated T-DNA border repeat sequences (Jayaswal et al., 1987; Wang et al., 1987). The VirD2 protein covalently links to the 5' end of the processed single-strand DNA (the T-strand) and leads it out of the bacterium, into the plant cell, and to the plant nucleus (Ward and Barnes, 1988; Howard et al., 1992).

(3) Secreting T-DNA and Vir proteins from the bacterium via a type IV secretion system (virB operon and virD4). The Agrobacterium virB operon contains 11 genes, most of which form a pore through the bacterial membrane for the transfer of Vir proteins (Christie et al., 2005). Currently, we know of five such proteins that are secreted through this apparatus: VirD2 (unattached or attached to the T-strand), VirD5, VirE2, VirE3, and VirF (Vergunst et al., 2000, 2005). VirD4 acts as a coupling factor to link VirD2-T-strand to the type IV secretion apparatus (Christie et al., 2005).

(4) Participating in events within the host cell involving T-DNA cytoplasmic trafficking, nuclear targeting, and integration into the host genome (virD2, virD5, virE2, virE3, and virF). VirD2 and VirE2 may play roles in targeting the T-strand to the nucleus (Howard et al., 1992; Zupan et al., 1996). In addition, VirE2 likely protects T-strands from nucleolytic degradation in the plant cell (Yusibov et al., 1994; Rossi et al., 1996). VirF may play a role in stripping proteins off the T-strand prior to T-DNA integration (Tzfira et al., 2004).

Although vir genes were first defined genetically because of their importance in virulence (Koekman et al., 1979; Garfinkel and Nester, 1980; Holsters et al., 1980; DeGreve et al., 1981; Leemans et al., 1981), no gene within T-DNA is essential for T-DNA transfer. The ability to delete wild-type oncogenes and opine synthase genes from within T-DNA and replace them with genes encoding selectable markers and other goi helped initiate the field of plant genetic engineering (Bevan et al., 1983; Fraley et al., 1983; Herrera-Estrella et al., 1983).

	DEVELOPMENT OF BINARY VECTOR SYSTEMS

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Initial efforts to introduce goi into T-DNA for subsequent transfer to plants involved cumbersome genetic manipulations to recombine these genes into the T-DNA region of Ti-plasmids (co-integrate or exchange systems; Garfinkel et al., 1981; Zambryski et al., 1983; Fraley et al., 1985; Fig. 1A ). This was because Ti/Ri-plasmids are very large, low copy number in Agrobacterium, difficult to isolate and manipulate in vitro, and do not replicate in Escherichia coli, the favored host for genetic manipulation. T-DNA regions from wild-type Ti-plasmids are generally large and do not contain unique restriction endonuclease sites suitable for cloning a goi. In addition, scientists wanted to eliminate oncogenes from T-DNA to regenerate normal plants. Opine synthase genes were also generally deemed superfluous in constructions designed to deliver goi to plants.

View larger version (17K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 1. Schematic diagram of co-integration/exchange systems and T-DNA binary vector systems to introduce genes into plants using Agrobacterium-mediated genetic transformation. A, Co-integration/exchange systems. Genes of interest (goi) are exchanged into the T-DNA region of a Ti-plasmid (either oncogenic or disarmed) via homologous recombination. Following exchange, the exchange/co-integration vector can be cured (removed) from the Agrobacterium cell; B, T-DNA binary vector systems. Genes of interest are maintained within the T-DNA region of a binary vector. Vir proteins encoded by genes on a separate replicon (vir helper) mediate T-DNA processing from the binary vector and T-DNA transfer from the bacterium to the host cell. The selection marker is used to indicate successful plant transformation. ori, Origin of replication; Abr, antibiotic-resistance gene used to select for the presence of the T-DNA binary vector in E. coli (during the initial stages of gene cassette construction) or in Agrobacterium.

The utility of binary systems for ease of genetic manipulation soon became obvious. No longer were complex, cumbersome microbial genetic technologies necessary to introduce a goi into the T-region of a Ti-plasmid. Rather, the goi could easily be cloned into small T-DNA regions within binary vectors specially suited for this purpose. After characterization and verification of the construction in E. coli, the T-DNA binary vector could easily be mobilized (by bacterial conjugation or transformation) into an appropriate Agrobacterium strain containing a vir helper region.

Over the past 25 years, both T-DNA binary vectors and disarmed Agrobacterium strains harboring vir helper plasmids have become more sophisticated and suited for specialized purposes. Table I lists many commonly used T-DNA binary vectors (and vector series). Table II lists many commonly used disarmed Agrobacterium vir helper strains.

	PROPERTIES OF BINARY VECTORS

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(1) T-DNA left and right border repeat sequences to define and delimit T-DNA. T-DNA border repeat sequences (T-DNA borders) contain 25 bp that are highly conserved in all Ti- and Ri-plasmids examined to date (Waters et al., 1991). Nicking by the VirD1/VirD2 endonuclease occurs between nucleotides 3 and 4 (Wang et al., 1987). Thus, within Agrobacterium, nucleotides 4 to 25 remain within the T-DNA at the left border (LB), whereas at the right border (RB) nucleotides 1 to 3 remain intact. However, within the plant, the T-strand is frequently chewed back, most likely by exonucleases. Because VirD2 is linked to and therefore protects the 5' end of the T-strand, loss of nucleotides at this end is usually minimal (a few nucleotides at most). Loss of nucleotides from the unprotected 3' end occurs more frequently and is generally more extensive; deletions up to several hundred nucleotides are not uncommon (Rossi et al., 1996). Early T-DNA binary vectors contained the plant antibiotic selection marker gene near the 5' end of T-DNA (RB), and goi were placed near the 3' end (LB; e.g. Bevan, 1984). However, extensive loss of DNA from the 3' end, most likely the result of nucleolytic degradation, could result in antibiotic-resistant transgenic plants with deletions in the goi. This problem was ameliorated by placing the selection marker gene near the LB and the goi near the RB. Extensive deletion of the T-DNA from the 3' end would result in removal of the selection marker and lack of recovery of these plants. Thus, deletion of the goi was generally abrogated. Sequences near RBs (so-called overdrive sequences) can increase transmission of T-DNA (Peralta et al., 1986). These sequences are frequently incorporated into T-DNA binary vector RB regions.

(2) A plant-active selectable marker gene (usually for antibiotic or herbicide resistance). The most commonly used selection systems employ aminoglycoside antibiotics such as kanamycin or hygromycin, herbicides such as phosphinothricin/gluphosinate, or herbicide formulations such as Basta or Bialophos. Other selection systems, such as phospho-mannose isomerase, employ metabolic markers (Todd and Tague, 2001). Some plant species have low-level tolerance to kanamycin, and care should be taken to determine the minimum concentration of antibiotic that will completely kill nontransformed tissues. As mentioned above, early binary vectors had these markers placed near the T-DNA RB. However, because of the polarity of T-DNA transfer (RB to LB; Wang et al., 1984), recent vectors contain the selectable marker near the LB to assure transfer of the goi.

(3) Restriction endonuclease, rare-cutting, or homing endonuclease sites within T-DNA into which goi can be inserted. Early binary vectors, such as pBIN19, contained a few restriction endonuclease cloning sites in a lacZ complementation fragment, permitting blue/white screening for the presence of the transgene insertion (Bevan, 1984). In many vectors, promoters and polyA addition signals flank these sites. More recently, binary vectors containing multiple rare-cutting restriction endonuclease or homing endonuclease sites have been developed (Chung et al., 2005; Tzfira et al., 2005). These vectors, derived from plasmids originally constructed by Goderis et al. (2002), are designed to accompany a series of satellite (pSAT) vectors. The pSAT vectors contain expression cassettes (promoter, multiple restriction endonuclease cloning sites, polyA addition signal) flanked by rare-cutting/homing endonuclease sites (Chung et al., 2005). Some of these vectors have incorporated into these expression cassettes tags to generate fluorescent fusion proteins for protein localization studies (Tzfira et al., 2005) or protein-protein interaction studies (Citovsky et al., 2006). Multiple expression cassettes from the pSAT vectors can be loaded into the cognate rare-cutting sites in the binary vectors, permitting simultaneous introduction of multiple genes into plants. Several recent binary vectors contain Gateway sites to facilitate insertion of genes or exchange of gene cassettes from other vectors. Additionally, several BAC binary vectors have been designed to clone large inserts of more than 100 kb (Hamilton, 1997; Liu et al., 1999, 2000).

(4) Origin(s) of replication to allow maintenance in E. coli and Agrobacterium. The incompatibility group of the plasmid, with function related to the specific origin of replication, can be important if several plasmids need to co-exist in the bacterium. As such, these plasmids must belong to different incompatibility groups. In some instances, origins of replication may function in both Agrobacterium and in E. coli (in which initial constructions are generally made). These broad host range replication origins include those from RK2 (incP; e.g. pBIN19 and derivatives), pSa (incW; e.g. pUCD plasmid derivatives), and pVS1 (e.g. pPZP derivatives). Other origins of replication that function in Agrobacterium, such as those from Ri-plasmids (e.g. pCGN vectors), do not function in E. coli; thus, a ColE1 origin (such as the one used in pUC and pBluescript plasmids) is added to the vector. Different origins of replication replicate to different extents in Agrobacterium. The pSa origin replicates to two to four copies per cell (Lee and Gelvin, 2004), the RK2 (Veluthambi et al., 1987) and pVS1 (L.-Y. Lee, unpublished data) origins replicate to seven to 10 copies per cell, and the pRi origin replicates to 15 to 20 copies per cell (L.-Y. Lee, unpublished data).

(5) Antibiotic-resistance genes within the chromosome and within backbone sequences for selection of the binary vector in E. coli and Agrobacterium. Many commonly used Agrobacterium strains are resistant to rifampicin due to a chromosomal mutation (see Table II). In addition, commonly used Agrobacterium strains can be grown on Suc as the sole carbon source. Most commonly used E. coli K12 laboratory strains cannot use Suc as a carbon source. Thus, growth on minimal medium containing rifampicin and Suc generally will eliminate E. coli from Agrobacterium cultures, an especially useful selection following introduction of the binary vector into Agrobacterium by mating plasmids between E. coli and Agrobacterium (Ditta et al., 1980; Garfinkel et al., 1981).

Care must be taken in matching binary vectors with specific vir helper Agrobacterium strains. As listed in Table II, many of these strains already express genes for resistance to kanamycin, carbenicillin, erythromycin, or gentamicin. Thus, one cannot easily use binary vectors with the same selection marker in these strains. For example, many T-DNA binary vectors based upon pBIN19 utilize kanamycin-resistance as the bacterial selection marker. A. tumefaciens EHA101 is kanamycin resistant and cannot easily be used with these pBIN19 derivatives. However, one can use these binary vectors in the near-isogenic kanamycin-sensitive strain A. tumefaciens EHA105. In addition, some Agrobacterium strains are resistant to low levels of spectinomycin, an antibiotic that is used in conjunction with the pPZP plasmids and their derivatives. When using spectinomycin, the researcher should test various concentrations of the antibiotic with the vir helper strain lacking the binary vector to assure effective killing. Care must also be taken if a binary vector contains a tetracycline-resistance gene. A. tumefaciens C58 harbors a tetracycline-resistance determinant (Luo and Farrand, 1999) and is thus resistant to low levels of this antibiotic.

Although some Agrobacterium strains or binary vectors may harbor a β-lactamase gene that confers resistance to carbenicillin, it is still relatively easy to kill these bacteria following infection of plants. The β-lactam antibiotics Augmentin and Timentin contain, additionally, clavulanate, which will inhibit β-lactamases. Concentrations of Timentin ranging from 100 to 150 mg/L will completely eliminate growth of Agrobacterium C58-based strains harboring a β-lactamase gene (Cheng et al., 1998). Agrobacterium Ach5-based strains, such as LBA4404, do not express β-lactamase activity well, and thus can be killed by even lower concentrations of either carbenicillin or Timentin (Hooykaas, 1988).

	ALTERNATIVE T-DNA BINARY SYSTEMS

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Although T-DNA binary vector systems almost always consist of T-DNA and vir regions localized on plasmids, it is not essential that they function this way. Replicons containing T-DNA or vir genes do not need to be plasmids. Indeed, several laboratories have shown that T-DNA can be integrated into an Agrobacterium chromosome and launched from this replicon (Hoekema et al., 1984; Miranda et al., 1992), and specialized vectors have been generated to facilitate integration of DNA into a specific neutral (i.e. not involved in virulence) region of the chromosome of A. tumefaciens C58 (Lee et al., 2001). Although launching T-DNA from the Agrobacterium chromosome can result in lower transformation frequencies, this process has the beneficial consequences of reducing integrated transgene copy number and almost completely eliminating integration of vector backbone sequences into the plant genome (Ye et al., 2007).

	CONCLUSION

TOP DEVELOPMENT OF BINARY VECTOR... PROPERTIES OF BINARY VECTORS ALTERNATIVE T-DNA BINARY SYSTEMS CONCLUSION LITERATURE CITED

 
T-DNA binary systems have greatly simplified the generation of transgenic plants. No longer are complex, sophisticated microbial genetic regimens required to integrate goi into T-DNA regions located on large, cumbersome Ti- or Ri-plasmids. Along with companion vir helper strains, numerous different T-DNA binary vectors with specialized properties have been designed to facilitate such diverse activities as protein expression, activation tagging, protein localization, protein-protein interaction studies, and RNAi-mediated gene silencing. However, the ease of use of binary vectors may have come at a cost. The use of multicopy binary vectors generally results in integration of multiple copies of T-DNA into the plant genome. Multiple transgene copies have a propensity to silence to a greater extent than do single integrated copies. In addition, integration of vector backbone sequences from binary vectors into plant DNA, a potential regulatory problem, is common (Martineau et al., 1994; Kononov et al., 1997; Wenck et al., 1997). Integration of non-T-DNA region sequences when T-DNA is launched from large Ti-plasmids is relatively rare (Ramanathan and Veluthambi, 1995). Thus, the use of multicopy binary vectors may have exacerbated two common problems associated with plant transformation, multiple integrated transgene copy number and vector backbone integration. Launching T-DNA from low-copy-number T-DNA binary vectors or from the Agrobacterium chromosome may mitigate these problems (Ye et al., 2007). Such systems should greatly increase the quality of Agrobacterium-mediated transformation events.

	ACKNOWLEDGMENTS

 
Work in the authors' laboratory is supported by the Biotechnology Research and Development Corporation, the Corporation for Plant Biotechnology Research, and the National Science Foundation (Plant Genome grant no. 0110023).

Received November 9, 2007; accepted November 25, 2007; published February 6, 2008.

	FOOTNOTES

 
www.plantphysiol.org/cgi/doi/10.1104/pp.107.113001

^* Corresponding author; e-mail gelvin{at}bilbo.bio.purdue.edu.

	LITERATURE CITED

TOP DEVELOPMENT OF BINARY VECTOR... PROPERTIES OF BINARY VECTORS ALTERNATIVE T-DNA BINARY SYSTEMS CONCLUSION LITERATURE CITED

 
Abuodeh RO, Orbach MJ, Mandel MA, Das A, Galgiani JN (2000) Genetic transformation of Coccidioides immitis facilitated by Agrobacterium tumefaciens. J Infect Dis 181: 2106–2110[CrossRef][ISI][Medline]

An G (1987) Binary Ti vectors for plant transformation and promoter analysis. Methods Enzymol 153: 292–305[CrossRef][ISI]

An G, Watson BD, Stachel S, Gordon MP, Nester EW (1985) New cloning vehicles for transformation of higher plants. EMBO J 4: 277–284[ISI][Medline]

Anderson A, Moore L (1979) Host specificity in the genus Agrobacterium. Phytopathology 69: 320–323[CrossRef][ISI]

Becker D (1990) Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucleic Acids Res 18: 203[Free Full Text]

Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12: 8711–8721[Abstract/Free Full Text]

Bevan MW, Flavell RB, Chilton MD (1983) A chimeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184–187[CrossRef]

Bouchez D, Camilleri C, Caboche M (1993) A binary vector based on Basta resistance for in planta transformation of Arabidopsis thaliana. C R Acad Sci Ser III Sci Vie 316: 1188–1193

Bulgakov VP, Kisselev KV, Yakovlev KV, Zhuravlev YN, Gontcharov AA, Odintsova NA (2006) Agrobacterium-mediated transformation of sea urchin embryos. Biotechnol J 1: 454–461[CrossRef][Medline]

Bundock P, den Dulk-Ras A, Beijersbergen A, Hooykaas PJJ (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14: 3206–3214[ISI][Medline]

Cangelosi GA, Hung L, Puvanesarajah V, Stacey G, Ozga DA, Leigh JA, Nester EW (1987) Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. J Bacteriol 169: 2086–2091[Abstract/Free Full Text]

Cangelosi GA, Martinetti G, Leigh JA, Lee CC, Theines C, Nester EW (1989) Role of Agrobacterium tumefaciens chvA protein in export of beta-1,2-glucan. J Bacteriol 171: 1609–1615[Abstract/Free Full Text]

Chakrabarty R, Banerjee R, Chung SM, Farman M, Citovsky V, Hogenhout SA, Tzfira T, Goodin M (2007) pSITE vectors for stable integration or transient expression of autofluorescent protein fusions in plants: probing Nicotiana benthamiana-virus interactions. Mol Plant Microbe Interact 20: 740–750[CrossRef][ISI][Medline]

Chen QJ, Zhou HM, Chen J, Wang XC (2006) A Gateway-based platform for multigene plant transformation. Plant Mol Biol 62: 927–936[CrossRef][ISI][Medline]

Cheng ZM, Schnurr JA, Dapaun JA (1998) Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Rep 17: 646–649[CrossRef][ISI]

Chung SM, Frankman EL, Tzfira T (2005) A versatile vector system for multiple gene expression in plants. Trends Plant Sci 10: 357–361[CrossRef][ISI][Medline]

Christie PJ, Atmakuri K, Krishnamoorthy V, Jakubowski S, Cascales E (2005) Biogenesis, architecture, and function of bacterial Type IV secretion systems. Annu Rev Microbiol 59: 451–485[CrossRef][ISI][Medline]

Citovsky V, Lee LY, Vyas S, Glick E, Chen MH, Vainstein A, Gafni Y, Gelvin SB, Tzfira T (2006) Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J Mol Biol 362: 1120–1131[CrossRef][ISI][Medline]

Coutu C, Brandle J, Brown D, Brown K, Miki B, Simmonds J, Hegedus DD (2007) pORE: A modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res 16: 771–781[CrossRef][Medline]

Curtis MD, Grossniklaus U (2003) A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133: 462–469[Abstract/Free Full Text]

Day MJD, Ashurst JL, Dixon RA (1994) Plant expression cassettes for enhanced translational efficiency. Plant Mol Biol Rep 12: 347–357[CrossRef]

de Framond AJ, Barton KA, Chilton MD (1983) Mini-Ti: a new vector strategy for plant genetic engineering. Biotechnology (N Y) 5: 262–269

de Groot MJA, Bundock P, Hooykaas PJJ, Beijersbergen AGM (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16: 839–842[CrossRef][ISI][Medline]

DeCleene M, DeLey J (1976) The host range of crown gall. Bot Rev 42: 389–466[CrossRef]

DeGreve H, Decraemer H, Seurinck J, Van Montagu M, Schell J (1981) The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6S3. Plasmid 6: 235–248[CrossRef][ISI][Medline]

Ditta G, Stanfield S, Corbin D, Helinski DR (1980) Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347–7351[Abstract/Free Full Text]

Douglas CJ, Staneloni RJ, Rubin RA, Nester EW (1985) Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region. J Bacteriol 161: 850–860[Abstract/Free Full Text]

Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS (2006) Gateway-compatible vectors for plant functional genomics and proteomics. Plant J 45: 616–629[CrossRef][ISI][Medline]

Fraley RT, Rogers SG, Horsch RB, Eichholtz DA, Flick JS, Fink CL, Hoffmann NL, Sanders PR (1985) The SEV system: a new disarmed Ti plasmid vector system for plant transformation. Biotechnology (N Y) 3: 629–635[CrossRef]

Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, et al (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80: 4803–4807[Abstract/Free Full Text]

Garfinkel DJ, Nester EW (1980) Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol 144: 732–743[Abstract/Free Full Text]

Garfinkel DJ, Simpson RB, Ream LW, White FF, Gordon MP, Nester EW (1981) Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell 27: 143–153[CrossRef][ISI][Medline]

Gelvin SB (2003) Agrobacterium and plant transformation: the biology behind the "gene-jockeying" tool. Microbiol Mol Biol Rev 67: 16–37[Abstract/Free Full Text]

Gleave AP (1992) A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20: 1203–1207[CrossRef][ISI][Medline]

Goderis IJWM, De Bolle MFC, Francois IEJA, Wouters PFJ, Broekaert WF, Cammue BPA (2002) A set of modular plant transformation vectors allowing flexible insertion of up to six expression units. Plant Mol Biol 50: 17–27[CrossRef][ISI][Medline]

Goodin MM, Dietzgen RG, Schichnes D, Ruzin S, Jackson AO (2002) pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. Plant J 31: 375–383[CrossRef][ISI][Medline]

Hajdukiewicz P, Svab Z, Maliga P (1994) The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol Biol 25: 989–994[CrossRef][ISI][Medline]

Hamilton CM (1997) A binary-BAC system for plant transformation with high-molecular weight DNA. Gene 200: 107–116[CrossRef][ISI][Medline]

Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000a) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42: 819–832[CrossRef][ISI][Medline]

Hellens R, Mullineaux P, Klee H (2000b) A guide to Agrobacterium binary Ti vectors. Trends Plant Sci 5: 446–451[CrossRef][ISI][Medline]

Hepburn AG, White J, Pearson L, Maunders MJ, Clarke LE, Prescott AG, Blundy KS (1985) The use of pNJ5000 as an intermediate vector for the genetic manipulation of Agrobacterium Ti-plasmids. J Gen Microbiol 131: 2961–2969[Medline]

Herrera-Estrella L, DeBlock M, Messens E, Hernalsteens JP, Van Montagu M, Schell J (1983) Chimeric genes as dominant selectable markers in plant cells. EMBO J 2: 987–996[ISI][Medline]

Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180[CrossRef]

Hoekema A, Roelvink PW, Hooykaas PJJ, Schilperoort RA (1984) Delivery of T-DNA from the Agrobacterium tumefaciens chromosome into plant cells. EMBO J 3: 2485–2490[ISI][Medline]

Holsters M, Silva B, Van Vliet F, Genetello C, DeBlock M, Dhaese P, Depicker A, Inzé D, Engler G, Villarroel R, et al (1980) The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid 3: 212–230[CrossRef][ISI][Medline]

Hood EE, Gelvin SB, Melchers LS, Hoekema A (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res 2: 33–50[CrossRef][ISI][Medline]

Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168: 1291–1301[Abstract/Free Full Text]

Hooykaas PJJ (1988) Agrobacterium molecular genetics. In SB Gelvin, RA Schilperoort, eds, Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp A4–A13

Howard EA, Zupan JR, Citovsky V, Zambryski PC (1992) The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell 68: 109–118[CrossRef][ISI][Medline]

Jayaswal RK, Veluthambi K, Gelvin SB, Slightom JL (1987) Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens. J Bacteriol 169: 5035–5045[Abstract/Free Full Text]

Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907[ISI][Medline]

Karimi M, Inzé D, Depicker A (2002) GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci 7: 193–195[CrossRef][ISI][Medline]

Kelly BA, Kado CI (2002) Agrobacterium-mediated T-DNA transfer and integration into the chromosome of Streptomyces lividans. Mol Plant Pathol 3: 125–134[CrossRef]

Klee HJ, Yanofsky MF, Nester EW (1985) Vectors for transformation of higher plants. Biotechnology (N Y) 3: 637–642[CrossRef]

Koekman BP, Ooms G, Klapwijk PM, Schilperoort RA (1979) Genetic map of an octopine Ti plasmid. Plasmid 2: 347–357[CrossRef][ISI][Medline]

Koncz C, Schell J (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204: 383–396[CrossRef][ISI]

Kononov ME, Bassuner B, Gelvin SB (1997) Integration of T-DNA binary vector "backbone" sequences into the tobacco genome: Evidence for multiple complex patterns of integration. Plant J 11: 945–957[CrossRef][ISI][Medline]

Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc Natl Acad Sci USA 98: 1871–1876[Abstract/Free Full Text]

Lazo GR, Stein PA, Ludwig RA (1991) A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology (N Y) 9: 963–967[CrossRef][Medline]

Lee LY, Gelvin SB (2004) Osa protein constitutes a strong oncogenic suppression system that can block vir-dependent transfer of IncQ plasmids between Agrobacterium cells and the establishment of IncQ plasmids in plant cells. J Bacteriol 186: 7254–7261[Abstract/Free Full Text]

Lee LY, Humara JM, Gelvin SB (2001) Novel constructions to enable the integration of genes into the Agrobacterium tumefaciens C58 chromosome. Mol Plant Microbe Interact 14: 577–579[ISI][Medline]

Lee LY, Kononov ME, Bassuner B, Frame BR, Wang K, Gelvin SB (2007) Novel plant transformation vectors containing the superpromoter. Plant Physiol 145: 1294–1300[Abstract/Free Full Text]

Leemans J, Shaw C, Deblaere R, DeGreve H, Hernalsteens JP, Maes M, Van Montagu M, Schell J (1981) Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet 1: 149–164[Medline]

Li G, Zhou Z, Liu G, Zheng F, He C (2007) Characterization of T-DNA insertion patterns in the genome of rice blast fungus Magnaporthe oryzae. Curr Genet 51: 233–243[CrossRef][ISI][Medline]

Liu YG, Nagaki K, Fujita M, Kawaura K, Uozumi M, Ohigara Y (2000) Development of an efficient maintenance and screening system for large-insert genomic DNA libraries of hexaploid wheat in a transformation competent artificial chromosome (TAC) vector. Plant J 23: 687–695[CrossRef][ISI][Medline]

Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D (1999) Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning. Proc Natl Acad Sci USA 96: 6535–6540[Abstract/Free Full Text]

Luo ZQ, Farrand SK (1999) Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58. J Bacteriol 181: 618–626[Abstract/Free Full Text]

Martineau B, Voelker TA, Sanders RA (1994) On defining T-DNA. Plant Cell 6: 1032–1033[CrossRef][ISI][Medline]

Matthysse AG (1995) Genes required for cellulose synthesis in Agrobacterium tumefaciens. J Bacteriol 177: 1069–1075[Abstract/Free Full Text]

McBride KE, Summerfelt KR (1990) Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Mol Biol 14: 269–276[CrossRef][ISI][Medline]

McCullen CA, Binns AN (2006) Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer. Annu Rev Cell Dev Biol 22: 101–127[CrossRef][ISI][Medline]

Miranda A, Janssen G, Hodges L, Peralta EG, Ream W (1992) Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism. J Bacteriol 174: 2288–2297[Abstract/Free Full Text]

O'Connell KP, Handelsman J (1999) chvA locus may be involved in export of neutral cyclic beta-1,2 linked D-glucan from Agrobacterium tumefaciens. Mol Plant Microbe Interact 2: 11–16[Medline]

Ooms G, Hooykaas PJJ, Van Veen RJM, Van Beelan P, Regensburg-Tuink TJG, Schilperoort RA (1982) Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7: 15–29[CrossRef][ISI][Medline]

Palanichelvam K, Oger P, Clough SJ, Cha C, Bent AF, Farrand SK (2000) A second T-region of the soybean-supervirulent chrysopine-type Ti plasmid pTiChry5, and construction of a fully disarmed vir helper plasmid. Mol Plant Microbe Interact 13: 1081–1091[ISI][Medline]

Pazour GJ, Das A (1990) Characterization of the VirG binding site of Agrobacterium tumefaciens. Nucleic Acids Res 18: 6909–6913[Abstract/Free Full Text]

Peng WT, Lee YW, Nester EW (1998) The phenolic recognition profiles of the Agrobacterium tumefaciens VirA protein are broadened by a high level of the sugar binding protein ChvE. J Bacteriol 180: 5632–5638[Abstract/Free Full Text]

Peralta EG, Hellmiss R, Ream W (1986) Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid. EMBO J 5: 1137–1142[ISI][Medline]

Piers KL, Heath JD, Liang X, Stephens KM, Nester EW (1996) Agrobacterium tumefaciens-mediated transformation of yeast. Proc Natl Acad Sci USA 93: 1613–1618[Abstract/Free Full Text]

Ramanathan V, Veluthambi K (1995) Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA. Plant Mol Biol 28: 1149–1154[CrossRef][ISI][Medline]

Robertson JL, Holliday T, Matthysse AG (1988) Mapping of Agrobacterium tumefaciens chromosomal genes affecting cellulose synthesis and bacterial attachment to host cells. J Bacteriol 170: 1408–1411[Abstract/Free Full Text]

Rossi L, Hohn B, Tinland B (1996) Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens. Proc Natl Acad Sci USA 93: 126–130[Abstract/Free Full Text]

Rothstein SJ, Lahners KN, Lotstein RJ, Carozzi NB, Jayne SM, Rice DA (1987) Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation. Gene 53: 153–161[CrossRef][ISI][Medline]

Saenkham P, Eiamphungporn W, Farrand SK, Vattanaviboon P, Mongkolsuk S (2007) Multiple superoxide dismutases in Agrobacterium tumefaciens: functional analysis, gene regulation and their influence on tumoriogenesis. J Bacteriol 189: 8807–8817[Abstract/Free Full Text]

Stachel SE, Nester EW, Zambryski PC (1986) A plant cell factor induces Agrobacterium tumefaciens vir gene expression. Proc Natl Acad Sci USA 83: 379–383[Abstract/Free Full Text]

Todd R, Tague BW (2001) Phosphomannose isomerase: a versatile selectable marker for Arabidopsis thaliana germ-line transformation. Plant Mol Biol Rep 19: 307–319[CrossRef][ISI]

Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, Krichevsky A, Taylor T, Vainstein A, Citovsky V (2005) pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants. Plant Mol Biol 57: 503–516[CrossRef][ISI][Medline]

Tzfira T, Vaidya M, Citovsky V (2004) Involvement of targeted proteolysis in plant genetic transformation by Agrobacterium. Nature 431: 87–92[CrossRef][Medline]

Uberlacker B, Werr W (1996) Vectors with rare-cutter restriction enzyme sites for expression of open reading frames in transgenic plants. Mol Breed 2: 293–295[CrossRef]

van Engelen FA, Molthoff JW, Conner AJ, Nap JP, Pereira A, Stiekema WJ (1995) pBINPLUS: an improved plant transformation vector based on pBIN19. Transgenic Res 4: 288–290[CrossRef][ISI][Medline]

Veluthambi K, Jayaswal RK, Gelvin SB (1987) Virulence genes A, G, and D mediate the double-stranded border cleavage of T-DNA from the Agrobacterium Ti plasmid. Proc Natl Acad Sci USA 84: 1881–1885[Abstract/Free Full Text]

Vergunst AC, Schrammeijer B, den Dulk-Ras A, de Vlaam CMT, Regensburg-Tuink TJG, Hooykaas PJJ (2000) VirB/D4-dependent protein translocation from Agrobacterium into plant cells. Science 290: 979–982[Abstract/Free Full Text]

Vergunst AC, van Lier MCM, den Dulk-Ras A, Stuve TAG, Ouwehand A, Hooykaas PJJ (2005) Positive charge is an important feature of the C-terminal transport signal of the VirB/D4-translocated proteins of Agrobacterium. Proc Natl Acad Sci USA 102: 832–837[Abstract/Free Full Text]

Vilaine F, Casse-Delbart F (1987) A new vector derived from Agrobacterium rhizogenes plasmids: a micro-Ri plasmid and its use to construct a mini-Ri plasmid. Gene 55: 105–114[CrossRef][ISI][Medline]

Wang K, Herrera-Estrella L, Van Montagu M, Zambryski P (1984) Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome. Cell 38: 455–462[CrossRef][ISI][Medline]

Wang K, Stachel SE, Timmerman B, Van Montagu M, Zambryski PC (1987) Site-specific nick in the T-DNA border sequence as a result of Agrobacterium vir gene expression. Science 235: 587–591[Abstract/Free Full Text]

Ward ER, Barnes WM (1988) VirD2 protein of Agrobacterium tumefaciens very tightly linked to the 5' end of T-strand DNA. Science 242: 927–930[ISI]

Waters VL, Hirata KH, Pansegrau W, Lanka E, Guiney DG (1991) Sequence identity in the nick regions of IncP plasmid transfer origins and T-DNA borders of Agrobacterium Ti plasmids. Proc Natl Acad Sci USA 88: 1456–1460[Abstract/Free Full Text]

Wenck A, Czako M, Kanevski I, Marton L (1997) Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation. Plant Mol Biol 34: 913–922[CrossRef][ISI][Medline]

Winans SC (1991) An Agrobacterium two-component regulatory system for the detection of chemicals released from plant wounds. Mol Microbiol 5: 2345–2350[CrossRef][ISI][Medline]

Xiang C, Han P, Lutziger I, Wang K, Oliver DJ (1999) A mini binary vector series for plant transformation. Plant Mol Biol 40: 711–717[CrossRef][ISI][Medline]

Xu XQ, Pan SQ (2000) An Agrobacterium catalase is a virulence factor involved in tumorigenesis. Mol Microbiol 35: 407–414[CrossRef][ISI][Medline]

Yadav NS, Van der Leyden J, Bennett DR, Barnes WM, Chilton MD (1982) Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc Natl Acad Sci USA 79: 6322–6326[Abstract/Free Full Text]

Ye X, Gilbertson A, Peterson MW, inventors. March 29, 2007. Vectors and methods for improved plant transformation efficiency. US Patent Application No. US2007/0074314 A1

Yusibov VM, Steck TR, Gupta V, Gelvin SB (1994) Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells. Proc Natl Acad Sci USA 91: 2994–2998[Abstract/Free Full Text]

Zambryski P, Joos PH, Genetello C, Leemans J, Van Montagu M, Schell J (1983) Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J 2: 2143–2150[ISI][Medline]

Zupan JR, Citovsky V, Zambryski P (1996) Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells. Proc Natl Acad Sci USA 93: 2392–2397[Abstract/Free Full Text]

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