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EDITOR'S CHOICE SERIES ON THE NEXT GENERATION OF BIOTECH CROPS

Bacterial RNA Chaperones Confer Abiotic Stress Tolerance in Plants and Improved Grain Yield in Maize under Water-Limited Conditions^[W]

Monsanto Company, Mystic Research, Mystic, Connecticut 06355 (P.C., D.W., R.J.B., D.C.A., C.B., M.H.L., J.E.H.); Monsanto Company, Chesterfield, Missouri 63017 (J.H., M.S., M.A., R.D., S.N., S.B., M.F.); Monsanto Company, Malleswaram, Bangalore, 560003 India (G.K., J.T., S.D.); and Monsanto Company, Cambridge, Massachusetts 02139 (S.S.)

Limited available water is the single most important factor that reduces global crop yields, with far reaching socioeconomic implications. In North America alone, it is estimated that 40% of yearly maize (Zea mays) crop losses are due to suboptimal water availability (Boyer, 1982). Agriculture currently accounts for 70% of the fresh water used by humans. This rate of water use can exceed local regeneration rates, often relying on underground aquifers that are rapidly being depleted (Morison et al., 2008). The impending scarcity of water available for agriculture will surely increase overall costs of crop production and drive the need for crops that use water more efficiently. While tremendous progress has been made through breeding and through cultural practices that improve maize yields in water-limited environments, the potential for additional large improvements still exists, and positive impacts on yield and increased yield stability across a broad range of water availability is of great value to farmers, consumers, and the environment.

Maize plants are sensitive to water-deficit stress throughout the growing season. Stresses that occur during the flowering stage, either just before floral initiation or immediately after pollination, result in the most significant reductions in end-of-season grain yields (Claassen and Shaw, 1970; Boyer and Westgate, 2004). Water-deficit stress during the vegetative growth phases typically leads to reductions in overall productivity, resulting in grain loss through reductions in kernel numbers. Late-stage drought stress, during the grain-filling period, can frequently lead to reductions in yield by reducing kernel size as well as increasing rates of kernel abortion, depending upon the severity of the stress.

Improved plant performance under severe water-limited growth chamber and greenhouse conditions has been achieved through multiple transgenic approaches, including the use of osmotic protectants such as the sugar alcohols trehalose (Romero et al., 1997), mannitol (Tarczynski et al., 1993), galactinol (Taji et al., 2002), and ononitol (Sheveleva et al., 1997). Accumulation of zwitterionic compounds such as Pro (Kishor et al., 1995) and Gly-betaine (Rathinasabapathi et al., 1994), or of protein protectants such as HVA1 (Xu et al., 1996), have all demonstrated an ability to confer tolerance to a variety of abiotic stresses. To date, none of the above approaches has been shown to provide durable tolerance in an agriculture production setting. Expression of a maize CAAT box transcription factor, ZmNF-YB2, has been shown to confer drought tolerance and enhanced photosynthetic capacity under drought stress with improvements in grain yield observed across several growing seasons in maize (Nelson et al., 2007). These studies clearly demonstrate that plants are amenable to improved stress tolerance through multiple mechanisms of action.

Biotechnology approaches facilitate our ability to survey and capitalize on the extensive genetic diversity that exists in nature and to improve on conserved pathways important for adaptation to environmental stress. Adaptation requires rapid recovery in growth and maintenance of cellular function following stress. We have looked to model systems such as bacteria and plants for insights into adaptive stress response pathways and commonalities among stress response mechanisms broadly in search of candidates for crop improvement. In this article, we demonstrate that expression of related cold shock proteins (CSPs) from bacteria, CspA from Escherichia coli and CspB from Bacillus subtilis, promotes stress adaptation in multiple plant species. Interestingly, expression of CSP proteins in maize is not associated with negative pleiotropic effects, indicating that stress tolerance does not come at a cost to crop productivity under well-watered conditions.

RNA chaperones are ubiquitous and abundant proteins found in all living organisms and viruses. RNA tends to be kinetically trapped in misfolded forms, and RNA binding proteins, acting as chaperones, can resolve these structures, ensuring accessibility for its biological function. In bacteria, RNA chaperones are believed to play a general role in sustaining active growth by favoring active transcription, translation, and/or ribosome assembly. In E. coli, cell growth is arrested by cold shock, and the arrest is associated with a significant reduction in protein synthesis (Etchegaray et al., 1996; Etchegaray and Inouye, 1999). A small set of proteins rapidly accumulates during the cold shock (for review, see Gualerzi et al., 2003; Horn et al., 2007). These proteins have been designated as CSPs and can account for as much as 10% of the newly synthesized protein of cold-shocked cells (Goldstein et al., 1990). One such example of a CSP protein is the E. coli protein CspA, which contains a prototypical cold shock domain (CSD) that is composed of 65 to 70 amino acid residues and has been reported in bacteria, archaea, and eukaryotes, including plants (for review, see Karlson and Imai, 2003; Weber and Marahiel, 2003; Horn et al., 2007). In bacteria, the CSP proteins are 7 to 10 kD in size and contain the nucleic acid binding activity sufficient for their function as RNA chaperones. The CSD contains a polynucleotide binding function, and in E. coli CspA is reported to act as an RNA chaperone where it binds to and, when necessary, converts double-stranded RNA into single-stranded RNA with low sequence selectivity (Jiang et al., 1997). The chaperone function of CspA, as well as other CSP proteins, is thought to be important for stimulating growth following stress acclimation and during periods of high metabolic activity. A recently proposed model describes CSPs working in conjunction with a DEAD box helicase to rescue misfolded mRNA molecules and maintain proper initiation of translation (Hunger et al., 2006). This model is consistent with previously reported evidence for the colocalization of CSPs with ribosomes in transcriptionally active cells, where CSP proteins are proposed to be implicated in coupling transcription with translation (Mascarenhas et al., 2001; Weber et al., 2001; for review, see El-Sharoud and Graumann, 2007).

Several reports described below support the hypothesis that the endogenous function of CSPs in plants relies on RNA binding/chaperone activity through the CSD and that these proteins, similarly to bacteria, regulate stress responses through a posttranscriptional mechanism. Mussgnug et al. (2005) describe the function of NAB1, a CSD-containing RNA chaperone in Chlamydomonas that links its RNA binding/chaperone activity with a target protein important for high light acclimation. NAB1 plays a role in high light acclimation by regulating the size of the light-harvesting antennae of PSII through posttranscriptional control of light-harvesting chlorophyll binding protein. Binding of NAB1 protein stabilizes light-harvesting chlorophyll binding protein mRNA at the preinitiation level via sequestration or masking and thereby suppresses translation. NAB1 activity accounts for approximately 50% of the translational repression of the protein, suggesting a mechanism that fine tunes expression at a posttranscriptional level and allows for very rapid response to occur under changing environmental conditions. The CSD domain of NAB1 was determined to be necessary and sufficient for specific high-affinity binding to this target. This RNA masking function was described previously in Xenopus oocytes for another CSD-containing protein, FRGY2 (Matsumoto et al., 1996; Manival et al., 2001).

In higher plants, Karlson et al. (2002) reported that wheat (Triticum aestivum) expresses a homolog of E. coli CSPA. The researchers found that this homolog, WCSP1, contains two RNA binding domains and increases in concentration during cold treatment. Kim et al. (2007) investigated a Gly-rich RNA binding protein from Arabidopsis (Arabidopsis thaliana), GRP2, and demonstrated that this protein plays a role in salt and cold stress adaptation. The researchers also found that GRP2 can rescue cold-sensitive E. coli CSP quadruple knockout and exhibits other properties consistent with an RNA binding/chaperone function. AtCSP2 (synonymous with GRP2) is also associated with floral transition and seed development (Fusaro et al., 2007; Sasaki et al., 2007). The possibility that CSPs functions in stress adaptation at least partially mediated by its role in the development and protection of reproductive structures should be explored. In maize, floral transition and reproductive development stages are the most sensitive to the water deficit stress conditions in terms of yield impact.

In this article, we demonstrate that bacterial CSPs can confer improved stress adaptation to multiple plant species. The action of CSPs in plants through a conserved stress adaptation mechanism common to plants and bacteria is supported by data showing that a functional RNA binding motif is required for the improved stress tolerance in both E. coli and maize. Stress tolerance at both vegetative and reproductive stages is reported with enhanced yield stability observed in maize under water-limiting conditions that were either imposed at various stages during plant development through controlled irrigation or occurred naturally in the western dryland region of the U.S. corn belt. Breadth of tolerance across environments and germplasms are key elements in establishing the value of transgenic strategies for crop stress tolerance improvement and require years of rigorous field testing to characterize the potential benefits.

	ARABIDOPSIS COLD TOLERANCE

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The expression of bacterial CSPs was shown to improve cold tolerance in transgenic Arabidopsis seedlings germinated and grown at low temperatures on standard agar media in petri dishes (Fig. 1 ). Transgenic Arabidopsis seedlings expressing CspA and CspB were tested for improved growth under low temperature conditions, using nontransgenic seedlings as controls. Following a 6-week treatment of 8°C under constant light, seedlings were visually scored for relative growth. At the end of the treatment period, a visual improvement in growth was noted for both transgenes, relative to their negative controls. This proof-of-concept for CSPs, demonstrating improved cold tolerance in a dicot plant species, was followed by similar abiotic stress experiments in transgenic rice (Oryza sativa).

View larger version (71K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 1. Transgenic Arabidopsis seedlings demonstrate improved growth under constant light conditions at 8°C for 6 weeks. Transgenic Arabidopsis seedlings expressing CspA or CspB are displayed on the right and exhibit more growth under these conditions relative to nontransgenic controls. A, CspA-negative control. B, CspA-positive transgenic. C, CspB-negative control. D, CspB-positive transgenic. The positive growth effect was only observed under the chilling stress conditions and not at 25°C (data not shown). Experimental details are described in Supplemental Materials and Methods S1.

	RICE COLD, HEAT, AND WATER-DEFICIT TOLERANCE

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A water-limited treatment led to a 50% reduction in the final plant height of nontransgenic control plants relative to well-watered controls. CspB conferred improved tolerance to water deficits as demonstrated by greater final plant heights in transgene-positive plants. At the end of the water-limited treatment, three of four CspB events tested were significantly taller (P < 0.05) than their nontransgenic controls. One event demonstrated significant improvements under all three stress treatments, and several other events showed positive trends across multiple treatments. Overall, this demonstrated that expression of CspB in rice results in tolerance to multiple abiotic stresses, and this tolerance occurs with fairly high frequency in the individual stress treatments.

For CspA-positive rice plants, cold and heat treatments also resulted in improved growth, as measured by greater final plant height. The treatment impact on plant height at the end of the recovery period resulted in a 46% and 51% reduction in plant height of nontransgenic control plants for the cold and heat treatments, respectively. Four of seven CspA events demonstrated significantly greater final plant height than their nontransgenic controls in the cold treatment, while three of seven CspA events demonstrated significantly greater final plant height compared to their nontransgenic control plants under the heat stress treatment. CspA rice plants were not evaluated in a water-deficit treatment.

Thus, the cold tolerance we demonstrated in transgenic Arabidopsis, a dicot, was also observed in rice, a monocot. Furthermore, the improved stress tolerance was extended beyond the cold tolerance that we observed in Arabidopsis and shown to also include heat and water-deficit tolerance. The rice water-deficit results, in particular, present a compelling outcome that drove further abiotic stress testing of transgenic CspA and CspB in maize.

	MAIZE VEGETATIVE WATER-DEFICIT TOLERANCE

TOP ARABIDOPSIS COLD TOLERANCE RICE COLD, HEAT, AND... MAIZE VEGETATIVE WATER-DEFICIT... MAIZE REPRODUCTIVE TOLERANCE AND... A Functional RNA Binding... DATA SUMMARY AND CONCLUSIONS LITERATURE CITED

 
An important factor in the success of a field testing program is the ability to accurately collect phenotypic data under predictable and consistent water deficit conditions in a managed stress environment. This has been achieved through a field testing network located in rain-free environments, allowing precise control over irrigation levels. Herein, we have reported improved vegetative and reproductive performance in field-grown maize exposed to water deficits that were introduced during periods of vegetative growth and ovule and kernel development.

We have demonstrated that transgenic expression of CspB in maize plants contributes to improved vegetative performance. Twenty-two CspB events were evaluated in water-limited field trials using commercial grade hybrid corn in environments that received no rainfall during the target period for the water-deficit treatment, a span of 10 to 14 d immediately prior to flowering. The water-deficit treatment resulted in an average reduction in growth rates to 50% of the well-watered rate. Using an across-event analysis, the CspB transgenics demonstrated a 3.6% increase in leaf extension rates relative to nontransgenic controls (Table II ). The best performing events demonstrated growth rate increases of 12% and 24%. This growth rate improvement under water-limited conditions indicated that the CspB transgene was having a substantial positive impact on plant productivity during the vegetative phase of plant growth and development. The CspB-expressing plants also demonstrated significant improvements in chlorophyll content and photosynthetic rates (Table II). Across all events, chlorophyll content was increased by 2.5%, with the top two events exhibiting increases of 4.4% and 3.3%. The improvements to the photosynthetic rates were 3.6% across all events, with increases of 8.5% and 7.7% for the top two performing events. Similar observations have been made with transgenic maize plants expressing CspA under greenhouse conditions (data not shown). These measures of vegetative performance are key indicators of plant productivity and would be expected to enhance the overall yield potential of the crop. When plants were grown under nonstressed, fully irrigated, or rain-fed conditions in both the greenhouse and field, we did not detect any appreciable difference between CspA- or CspB-expressing lines and the isogenic control for plant growth rate or plant height measured at different stages of development (data not shown).

	MAIZE REPRODUCTIVE TOLERANCE AND YIELD UNDER WATER-DEFICIT CONDITIONS

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Grain yield trials were performed under water-deficit stress and nonstress conditions on 10 CspA and 10 CspB-positive events, most of which had previously demonstrated improved vegetative performance in either greenhouse screens or field trials. Grain yield data was collected from four field sites where water was limited during the late vegetative phase of development, a treatment similar to the initial water-deficit field trial. Mean yield at the water-limited sites was 6.8 tons (t)/ha, representing an approximately 50% reduction in yield relative to the average mean yield of crops in the Midwest. An across-event analysis demonstrates that the CspA transgenic entries provide a yield increase of 4.6% (P < 0.2) under water stress, with the two best performing events demonstrating advantages of 30.8% and 18.3% (Table III ). Yield averages of CspB-positive plants as a group were significantly greater than controls, by 7.5% (P < 0.01). A number of individual events exhibited significant yield advantages as well; the best two performing events, CspB-Zm event 1 and event 2, demonstrated yield improvements of 20.4% and 10.9%, respectively. These are the same two events that demonstrated significant improvements in leaf growth, chlorophyll content, and photosynthetic rates, providing evidence that these improvements in vegetative productivity will translate into improvements in reproductive performance and grain yield.

View larger version (20K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 2. A single transgenic CspB event demonstrates yield improvements across multiple years of testing under water-stressed environments. Performance of CspB-Zm event 1 (black diamonds) and the appropriate control (white circles) were plotted against the location average yields across the past three seasons of testing under managed stress environments. x axis, Average yield (tons/hectare) at each location; y axis, mean yield (tons/hectare) of the entries, by location. Experimental details are described in Supplemental Materials and Methods S1.

This same transgenic CspB event was tested under western dryland maize conditions without supplemental water. Hybrid entries were planted as 100-foot-long 4-row plots to better simulate normal agronomic conditions for this region, planting at appropriate population densities for those regions, and were paired with appropriate nontransgenic controls. Environmental data was collected and seasonal weather patterns, including rainfall accumulation, were utilized to classify the dryland locations as to whether each of the locations experienced water-deficit stress during the season. In the final analysis, 12 of the locations planted across the western dryland were categorized as having experienced water stress during the late vegetative through reproductive developmental stages and were utilized for analysis. As depicted in Figure 3 , yield benefits were observed in the same three hybrid backgrounds that were evaluated under controlled water-deficit conditions described in Table IV. When compared to the nontransgenic control, the CspB transgenic event provides yield benefits of up to 0.75 t/ha, or 15%. These dryland growing conditions created a lower yielding environment (average yield of the controls were 4.9 t/ha) than the controlled water-deficit locations where the overall yields of the controls ranged from 6.4 to 8.5 t/ha. The results from the managed stress environment treatments were very similar to the observations made under dryland growing conditions, highlighting the utility of the managed stress environmental testing platform.

View larger version (18K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 3. Yield results from Midwest evaluations under water-deficit conditions. Three different hybrids carrying a single transgenic event expressing CspB were evaluated in yield trials across the western dryland market. Yield results were averaged across locations that experienced water-deficit stress during the late vegetative or grain fill periods of the season. Experimental details are described in Supplemental Materials and Methods S1.

	A Functional RNA Binding Site Is Required to Confer Yield Benefits to Field-Grown Maize under Water Stress

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A well-documented functional aspect of CSPs, including CspB, is their ability to bind single-stranded DNA or single-stranded RNA that can be measured by the protein's ability to open a stem-loop or double-stranded dual-labeled fluorescence probe in vitro. By mutating a key residue in the RNA binding domain of B. subtilis CspB (CspB_F30R), we have reduced the ability of this protein to open a double-stranded stem loop structure engineered into the probe (Fig. 4 ). There was an approximately 10-fold reduction in fluorescence observed with the CspB_F30R mutant, suggesting that this residue is important for maintaining RNA chaperone activity. A similar result has been obtained through mutation of the identical residue in the E. coli CspE protein (Phadtare et al., 2002), and its activity in antitermination was similarly lost. The comparable mutation in the F31 residue of the E. coli CspA protein (Schröder et al., 1995) resulted in a similar loss of function. Moreover, the protein containing the F30R mutation no longer complements an E. coli mutant (BX04; Xia et al., 2001) that lacks four native CSPs and is highly sensitive to cold stress (data not shown). These results suggest that this protein has lost its ability to function as an RNA chaperone in E. coli.

View larger version (12K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 4. Single-stranded DNA probe opening ability for B. subtilis CspB and the CspB_F30R mutant. Probe opening assay for BsCspB, BsCspBF30R, and controls. Negative control contained all elements that Csp samples contained except protein. The average of three independent replications is represented (±SD). The y axis is labeled as the average relative fluorescence units (RFU). Experimental details are described in Supplemental Materials and Methods S1.

	DATA SUMMARY AND CONCLUSIONS

TOP ARABIDOPSIS COLD TOLERANCE RICE COLD, HEAT, AND... MAIZE VEGETATIVE WATER-DEFICIT... MAIZE REPRODUCTIVE TOLERANCE AND... A Functional RNA Binding... DATA SUMMARY AND CONCLUSIONS LITERATURE CITED

 
Future progress toward more sustainable agricultural practices will be accelerated through the systematic analysis of gene function important for yield under water-deficit conditions. Model systems research has provided a greater understanding of pathways of plant stress acclimation that is further providing valuable insights into crop responses under environmental stress conditions. Rapidly moving to large-scale analyses of candidate gene function in crops, under standard agronomic practices and where the influences of environmental factors and the ultimate impact on yield can be assessed, is critical for developing improved crops through biotechnology.

We have demonstrated that constitutive expression of two members of a family of bacterial RNA chaperones, E. coli CspA and B. subtilus CspB, was shown to confer abiotic stress tolerance in transgenic Arabidopsis, rice, and maize. Importantly, the improvements in water-limited field trials were not associated with a yield penalty in high-yielding environments. Consistent with the timing of the water deficit, the positive yield impact of the transgenes was predominately on kernel numbers, not on kernel weight (data not shown). This technology has been observed under different stress regimes and across environments as clearly providing performance benefits under late vegetative/flowering water deficit as well as during the grain fill period. During these two periods, water-deficit stress leading to three consecutive days of wilting can lead to 30% to 50% reductions in grain yields (Claassen and Shaw, 1970). The sensitivity of maize yield at these developmental stages and the frequency with which these conditions occur across the corn belt are consistent with the observed higher correlation of grain yield to kernel number and not kernel weight (Duvick, 2005). A multi-year analysis across the full range of stress conditions suggests that this technology provides broad yield stability under water-limiting conditions. In addition to the managed stress environment testing, a CspB transgenic event was tested under dryland conditions as well. Demonstration of consistent yield benefits under managed water environments and dryland conditions is an important advancement in the development of this technology. These yield improvements further outline the potential of this technology to provide a significant agricultural benefit. As these hybrids were configured with an elite transformation germplasm, a component of future evaluation of this technology will be testing of these transgenic events in germplasm that is adapted for the marketplace.

The potential utility of CSPs as transgenes is strongly supported by the demonstration that the two CSPs tested, which share only 61% overall identity, were both capable of improving stress tolerance in plants. As tested, we were not able to distinguish the performance of CspA from CspB, and we speculate that they are likely acting by similar mechanisms. The myriad of CSPs known in nature, and available for testing, and the possibility of improving on the current expression pattern and coding regions of these transgenes present intriguing possibilities for even further improvements.

Previous reports demonstrated that CSD protein functionality in vivo is significantly impaired by mutations that affect RNA binding. Nakaminami et al. (2006), for example, reported that site-directed mutagenesis of WCSP1 ribonucleoprotein (RNP) domains abolishes the ability of the protein to complement the E. coli cold-sensitive mutant BX04. Our observation that maize plants expressing the CspB_F30R single amino acid mutation do not exhibit improved yield under water-deficit conditions further supports the prediction that the biochemical function of these proteins as defined in bacterial systems is also important in plants. Testing the ability of other known CSP mutants for their ability to confer tolerance to water-deficit in plants will further define the structure-function relationships and improve our understanding of the mode of action of these proteins. Consistent with these hypotheses, we have evaluated the subcellular location of GFP protein fusions with CspA, CspB, and a cotton (Gossypium hirsutum) CSP transiently expressed in maize protoplasts. As depicted in Figure 5 , we observed GFP fluorescence in the cytosol, nucleus, and nucleolar region, consistent with observations of subcellular localization of plant and animal CSD proteins (Nakaminami et al., 2006; Sasaki et al., 2007). By comparison, expression of an Arabidopsis AP2-type transcription factor is restricted to the nucleus and nucleolar region, consistent with its function. Nucleoli are largely comprised of proteins and the ribosomal DNA sequences of chromosomes, surrounded by a layer of condensed chromatin. Because nucleoli are involved in the production and maturation of ribosomes, these suborganellar structures are extremely important during phases of rapid growth and development. Concentrations of CspA and CspB proteins in these regions as well as in the cytosolic regions of the cell are highly consistent with their proposed role in RNA binding and participation in RNPs.

View larger version (58K): [in this window] [in a new window] [as a PowerPoint slide]   Figure 5. CspA (A), CspB (B), and cotton CSP-like 1 (C) GFP fusion proteins accumulate in the cytoplasm, nucleus, and nucleolus when expressed in maize protoplasts. D, Results obtained with an AtCBF3 transcription factor:GFP fusion protein that localizes exclusively to the nucleus. Experimental details are described in Supplemental Materials and Methods S1.

Based on the yield and stress benefits we have observed from expression of CSD-containing proteins in plants and our current understanding of the common RNA chaperone function of CSD proteins in prokaryotes and eukaryotes, expression of CSD proteins from a variety of plant and bacterial sources represents a promising approach for stimulating growth and productivity in plants under conditions of abiotic stress. The fact that expression of CSP proteins did not result in detrimental effects on plant size, development, or productivity as previously observed for other transgenic approaches further supports the utility of these proteins. It is interesting to speculate why CSP functionality was not selected for over the years of conventional breeding. However, the low heritability of yield, the challenges associated with controlled environment testing, and the quantitative nature of the trait are likely contributing factors.

The stress tolerance conferred by CSPs represents a novel, compelling approach toward engineering improved plant productivity in suboptimal growth conditions. These studies have confirmed that this family of proteins is capable of delivering broad stress tolerance, which also translates to improvements in grain yield under both managed stress studies and marketplace environments. As water resources become increasingly scarce and the global demands for grain continue to increase, the ability to bring yield stability across water-limiting environments presents an important advancement in the area of stress tolerance research. The opportunity exists for the drought-tolerant trait to be added to a growing set of germplasm and trait options that mitigate environmental stresses on the corn plant and provide the crop with a better opportunity to reach its yield potential in any environment.

Sequence data from this article can be found in the GenBank/EMBL data libraries under accession numbers M30139 and U58859.

Supplemental Data

The following materials are available in the online version of this article.

Supplemental Materials and Methods S1.

	ACKNOWLEDGMENTS

 
This work could not have been accomplished without the assistance from Don Nelson, Tom R. Adams, Brendan Hinchey, and Adrian Lund. Valuable advice and consultation was received from Philip Miller, Tom H. Adams, Stan Dotson, Nordine Cheikh, Mark Lawson, Tom Peters, and Mike Stephens. We would also like to acknowledge the many people who supported this work, including the plant transformation, greenhouse staff, and farm network teams.

Mary Fernandes and Mark Abad contributed to the Arabidopsis work.

Ganesh Kumar, Jayaprakash Targolli, and Santanu Dasgupta contributed to the rice work.

Paolo Castiglioni, Don Anstrom, Robert Bensen, Dave Warner, Martin Stoecker, Jay Harrison, Christopher Bonin, Robert D'Ordine, Sara Salvador, Santiago Navarro, Stephanie Back, Michael Luethy, and Jacqueline Heard contributed to the maize work.

Received March 11, 2008; accepted April 21, 2008; published June 6, 2008.

	FOOTNOTES

 
¹ Present address: 1920 Fifth Street, Davis, CA 95616.

² Present address: 700 Chesterfield Parkway, Chesterfield, MO 63017.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Jacqueline E. Heard (jacqueline.e.heard{at}monsanto.com).

^[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.118828

^* Corresponding author; e-mail jacqueline.e.heard{at}monsanto.com.

	LITERATURE CITED

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