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First published online December 11, 2009; 10.1104/pp.109.150466 Plant Physiology 152:927-938 (2010) © 2010 American Society of Plant Biologists OPEN ACCESS ARTICLE
Aneuploidy Causes Tissue-Specific Qualitative Changes in Global Gene Expression Patterns in Maize1,[W],[OA]Biology Department, Hamline University, Saint Paul, Minnesota 55104
Segmental aneuploidy refers to the relative excess or deficiency of specific chromosome regions. This condition results in gene dosage imbalance and often causes severe phenotypic alterations in plants and animals. The mechanisms by which gene dosage imbalance affects gene expression and phenotype are not completely clear. The effects of aneuploidy on the transcriptome may depend on the types of cells analyzed and on the developmental stage. We performed global gene expression profiling to determine the effects of segmental aneuploidy on gene expression levels in two different maize (Zea mays) tissues and a detailed analysis of expression of 30 genes affected by aneuploidy in multiple maize tissues. Different maize tissues varied in the frequency at which genes located outside of the aneuploid regions are positively or negatively regulated as well as in the degree of gene dosage compensation. Multiple genes demonstrated qualitative changes in gene expression due to aneuploidy, when the gene became ectopically expressed or completely silenced in aneuploids relative to wild-type plants. Our data strongly suggested that quantitative changes in gene expression at developmental transition points caused by variation in gene copy number progressed through tissue development and resulted in stable qualitative changes in gene expression patterns. Thus, aneuploidy in maize results in alterations of gene expression patterns that differ between tissues and developmental stages of maize seedlings.
For most eukaryotic genomes, the balance in gene dosage is essential for normal function. Aneuploidy is a deviation from the normal chromosome number that involves the loss (monosomy) or gain (trisomy) of one or more individual chromosome(s) or large chromosomal segments (segmental aneuploidy) and results in a dosage imbalance of genes on the affected chromosome(s). Such imbalance can cause severe phenotypic syndromes in both plants and animals (for review, see Birchler and Veitia, 2007  ; Dierssen et al., 2009). In humans, all autosomal aneuploid conditions are either lethal or result in severe phenotypic syndromes that include mental retardation and multiple anomalies in development of internal organs (Epstein, 2001). Although plants are generally more tolerant to aneuploidy than animals (Matzke et al., 2003), aneuploid plants exhibit a variety of phenotypic syndromes, including developmental delays, partial sterility, and alterations in plant architecture (Birchler et al., 2001; Birchler and Veitia, 2007; Makarevitch et al., 2008). Interestingly, substantial variation exists in the ability of plants to tolerate gene dosage imbalance caused by aneuploidy, both between different plant species and between varieties of the same species (for review, see Henry et al., 2005, 2007). However, despite the widespread interest in aneuploidy, there is a limited understanding of the molecular mechanisms that lead to phenotypic alterations in aneuploid organisms as well as gene interactions involved in coping with gene dosage imbalance caused by aneuploidy on the global genomic level.
Several studies assayed RNA levels or activity of enzymes encoded by genes that were located within segmental aneuploid regions or were exposed to segmental aneuploidy of other chromosomal regions in maize (Zea mays; Birchler, 1979
Indirect comparisons of different cell types in the mouse model of Down syndrome and human patients suggest variation in response to aneuploidy between different tissues (Mao et al., 2003
Segmental aneuploid maize plants analyzed in this study carry three copies of a short arm of chromosome 5 (duplication) and only one copy of a small region of chromosome 6 (deficiency) and are referred to as duplicate-deficient (DpDf) plants. DpDf plants exhibit phenotypic abnormalities when compared with their siblings, including leaf knotting, developmental delay, partial tassel sterility, and growth reduction (Makarevitch et al., 2008 Here, we report the results of two experiments: (1) an investigation of effects of aneuploidy on global gene expression in meristem-enriched and leaf tissues using microarray analysis of over 15,000 maize genes, and (2) a study of gene expression changes in response to aneuploidy for 30 genes affected by aneuploidy in six different maize tissues and at three early developmental stages after germination. At least 23 out of 30 genes analyzed were either ectopically expressed or erroneously silenced in mature aneuploid tissues. Our data strongly suggest that quantitative changes in gene expression at developmental transition points caused by variation in gene copy number progress through tissue development and result in stable qualitative changes in gene expression patterns.
Aneuploidy Affects Global Gene Expression in a Tissue-Specific Manner
We were interested in comparing genome-wide expression profiles of DpDf plants that are trisomic for a short arm of chromosome 5 and monosomic for a small region of chromosome 6 and their diploid siblings in multiple tissues. Specifically, we wanted to address the tissue-specific differences in the frequency of primary or cis-effects (variation in the expression levels of genes located within the affected portions of the genome) and secondary or trans-effects (variation in the expression levels of diploid genes located elsewhere in the genome). We hypothesized that meristem tissues would show a larger degree of trans-effects, as a developmentally active tissue with an elaborate gene regulation network. In this study, we performed global gene expression profiling of aneuploid maize meristem-enriched tissues and compared these data with whole seedling expression data for these same genotypes from a previous study (Makarevitch et al., 2008
A series of statistical tests, ratio cutoffs, and expression level criteria (for detailed description of handling cutoffs and several normalization methods used in the analysis, see "Materials and Methods" ) were applied to identify a set of 1,447 genes that are differentially expressed in DpDf plants relative to wild-type siblings (Table I ; Supplemental Table S1). These 1,447 differentially expressed genes include 971 genes (6.5% of all genes present on the microarrays) that displayed higher transcript levels either in DpDf meristem-enriched tissue or in DpDf total seedlings and 474 genes (3.1% of genes) that displayed lower transcript levels in either one or both DpDf tissues (Table I). One gene was transcribed at a higher level in DpDf meristem-enriched tissues and at a lower level in DpDf total seedling tissues. In total, less than 10% of all the genes assessed by this platform demonstrated altered gene expression levels. In conformity with the expected dosage-dependent change in expression (1.5-fold increase in the trisomic region and 2-fold decrease in the monosomic region), the vast majority (1,411 of 1,447) of the differentially expressed genes exhibited less than a 2-fold increase in transcript levels in DpDf plants relative to wild-type siblings (Table I). Analysis of the Gene Ontology annotations for the differentially expressed genes did not reveal evidence for overrepresentation of any functional categories relative to their abundance on the microarray (data not shown). We also did not notice a correlation between the expression level of a gene and its likelihood to be sensitive to dosage changes in one or both tissues. Applying expression ratio cutoffs could potentially affect the number and distribution of genes identified as differentially expressed. To test for this possibility, we performed additional analyses for all genes displaying statistically significant differences in expression levels between DpDf and wild-type tissues (for details, see "Materials and Methods"; Supplemental Tables S2 and S3; Supplemental Text S1). This analysis indeed revealed a larger number of differentially affected genes (1,846 genes compared with 1,447). However, the proportion and distribution of trans- and cis-effects between different tissues remained very similar regardless of whether cutoffs were used or not.
Aneuploidy Affects Different Sets of Genes in Meristem-Enriched and Seedling Tissues Interestingly, the effects of aneuploidy on gene expression varied substantially in the two tissues that were assessed. Only 37% of the genes with higher transcript levels in DpDf plants (358 of 972) were differentially expressed in both meristem and seedling tissues, whereas 40% (385 of 972) and 24% (229 of 972) of the genes with higher transcript levels in DpDf plants were affected only in meristem-enriched tissue or whole seedlings, respectively. Even more strikingly, only 56 (12% of all genes with lower transcript levels in DpDf plants) genes were differentially expressed in both tissues, whereas 207 and 211 genes were affected only in meristem or whole seedling tissue, respectively (Table I). One possible explanation for these tissue-specific differences in aneuploidy effects could be that genes affected in only one tissue were not expressed at all in another tissue and therefore did not exhibit dosage-dependent differences in expression levels in the other tissue. However, the vast majority (over 85%) of genes differentially expressed in either meristem-enriched or whole seedling tissue were expressed in the other tissue (genes differentially expressed in one of the tissues and not expressed in another tissue are shown in parentheses in Table I). Another possible explanation for the tissue-specific differences could be the failure of some genes to reach a statistical threshold level, such that a particular gene was affected in both tissues but was only statistically significant in one of the tissues. Genes affected only in meristems or seedlings (shown in white squares or gray triangles in Fig. 1 ) could be clearly divided into two groups: likely affected in both tissues (located in the top right or bottom left quadrant) and affected only in one of two tissues (located along the axes). We performed t tests to identify genes that demonstrated statistically significant differences between ratios of gene expression in DpDf and wild-type seedlings in meristem-enriched tissues versus seedling tissues. Over 57% of genes expressed in both tissues and identified as differentially expressed only in one of the two tissues by microarrays demonstrated statistically significant differences (t test, P < 0.1) between meristem-enriched and seedling tissues (Fig. 1; Supplemental Table S1). Taken together, our microarray data suggested that the effects of aneuploidy on gene expression differ in meristem-enriched and predominantly leaf tissues.
Tissue-Specific Effects of Aneuploidy on Gene Expression Are Predominantly Due to trans-Effects The maize genome sequence and genetic and physical map resources were used to determine the locations of 10,143 genes probed with Affymetrix microarrays (67% of all genes present on microarrays; Table I, Fig. 2 ). In the subsequent analysis of aneuploidy effects on gene expression, we primarily focused on trisomic genes located on the short arm of chromosome 5. Although expression changes for the monosomic genes could cause some of the trans-effects and phenotypic alterations of DpDf plants, we believe that effects of the monosomic genes are likely less significant, since the monosomic region of chromosome 6 in DpDf plants is very short, with only 14 genes present on the arrays used in this study (less than 2% compared with trisomic genes located on the short arm of chromosome 5). The majority (418 of 752) of the genes with higher transcript levels in either tissue of DpDf plants with available map positions are located on the trisomic portion of chromosome 5 (contigs 204–238). However, the genomic localization of genes displaying higher transcript levels in DpDf plants varied depending on whether the genes exhibit a common response in both tissues or a tissue-specific expression response to aneuploidy. Over 91% (262 of 287) of the genes with higher transcript levels in both DpDf tissues are located in the segmental aneuploid portion of the genome (Table I). However, only 65% (107 of 164) or 16% (49 of 301) of the genes displaying higher transcript levels only in seedling or meristem, respectively, are located within the segmental aneuploid region (Table I). This suggests that aneuploidy-induced trans-effects are much more common in meristem tissue than in whole seedling tissues. Trans-effects were equally distributed across all chromosomes (Fig. 2; Fisher's exact test, P = 46%), indicating that segmental aneuploidy of a short arm of chromosome 5 can affect the expression levels of genes throughout the maize genome in a tissue-specific manner. The data also suggested that dosage-dependent alterations in expression levels of genes located in trisomic regions of chromosome 5 are often maintained in multiple tissues, whereas trans-effects involving genes from other chromosomes and diploid regions of chromosome 5 are more dependent on a particular tissue. Similar analyses for the monosomic region of chromosome 6 were inconclusive, because this region is very short and contains only 14 genes present on the microarrays. We did note that 10 out of 14 genes in this monosomic region exhibited lower expression in DpDf plants than in wild-type plants in one or both tissues, potentially causing some of the trans-effects reported in this study. Similar trends were noticed when only genes with higher than 2-fold changes in gene expression were analyzed (Table II ).
Approximately 50% of Trisomic Genes Exhibit Dosage Compensation in Each of Two Tissues Our data provided evidence for dosage compensation effects for some genes within the trisomic region of chromosome 5. Out of 694 trisomic genes expressed in either tissue and assayed by 17 K maize microarrays, 38% (265 of 694) of genes did not exhibit altered expression in any of two tissues, while another 38% (262 of 694) showed higher transcript levels in both DpDf tissues and 22% (156 of 694) demonstrated higher transcript levels in one of the two DpDf tissues studied (Table I; Figs. 2 and 3 ). To verify this general trend for trisomic genes, we used quantitative reverse transcription (qRT)-PCR to quantify transcript levels of five genes with higher transcript levels in DpDf plants and five dosage-compensated genes showing moderate and low expression. The qRT-PCR data supported the microarray data and suggested that some genes exhibit dosage-dependent up-regulation while other genes exhibit dosage compensation (Supplemental Table S4). Comparing gene expression ratios for genes located in contigs 204 through 225 and contigs 230 through 238 (Fig. 2) revealed a higher proportion of genes with higher transcript levels in either or both DpDf tissues in contigs 204 through 225. These data suggest that the actual breakpoint in the DpDf plants occurred somewhere between contigs 225 and 230, leading to overestimation of the number of dosage-compensated trisomic genes. Genes located in contigs 230 through 238 and considered trisomic in this study may actually be diploid, since most of the genes in this region, as opposed to contigs 204 through 225, did not exhibit changes in gene expression. Cytological and fluorescent in situ hybridization studies are necessary to confirm the actual location of the break point. However, even when these genes were taken into account, more than 45% of trisomic genes were dosage compensated in at least one of the DpDf tissues analyzed. Interestingly, despite the increased gene dosage, 11 genes located on the short arm of chromosome 5 had significantly lower expression levels in DpDf plants compared with the wild-type seedlings. Four of these 11 genes showed lower expression values in meristem-enriched DpDf tissues, while seven showed lower transcript levels in only whole seedling DpDf tissue. Whether the observed lower transcript levels in DpDf tissues are due to epigenetic silencing, altered transcription factor availability, or some other mechanism remains to be investigated.
Large Changes in Gene Expression Are Frequently Due to Qualitative Changes in Gene Expression Patterns Only 36 genes (0.2% of all genes present on the microarrays) showed a higher than 2-fold change in gene expression between DpDf and wild-type seedlings in either meristem-enriched or whole seedling tissue (Table II). A large proportion of the differentially expressed genes showing large fold changes demonstrated qualitative changes in the expression state (on/off) as opposed to quantitative changes in gene transcript levels (Table II). To more fully investigate the pattern of aneuploidy effects on gene expression, we performed qRT-PCR analysis for genes showing high fold changes in gene expression in at least one of the tissues.
We were intrigued by the tissue-specific effects of aneuploidy on gene expression levels and decided to further characterize this phenomenon in additional tissues. We selected 30 genes, including genes displaying both higher and lower transcript levels in either one or both DpDf tissues (Table III ), and analyzed their expression using qRT-PCR in six tissues: meristem, young leaf, developing leaf, mature leaf, root, and immature ear (for details of tissue collection, see "Materials and Methods"). The majority of these 30 genes showed clear tissue-dependent patterns of gene expression changes in response to aneuploidy (Table III; Fig. 4 ). For example, gene CF629635 was not affected in meristem-enriched tissues, demonstrated quantitatively higher transcript levels in all DpDf leaf tissues, and was turned on in immature ear tissue of DpDf plants (Table III). A gene was termed "turned on" when its transcript was absent in a wild-type tissue and present in an aneuploid tissue. Similarly, a gene was defined as "turned off" when its transcript was present in a wild-type tissue and absent in an aneuploid tissue. We defined the "absence" of the transcript as no detectable PCR product after 40 cycles of RT-PCR or noise-level amplification signal in qRT-PCR. Most of the genes (25 of 30) with large fold change differences in meristem or total seedlings exhibit qualitative (presence/absence) variation for gene expression between DpDf and wild-type plants in at least one tissue analyzed.
Our experiments revealed multiple examples of altered gene expression patterns in aneuploid tissues that progressed throughout leaf development. Although these meristem-specific genes were progressively turned off during leaf development in wild-type seedlings, they failed to turn off and remained expressed in mature leaf tissues of DpDf seedlings, thus resulting in qualitative changes in gene expression patterns. Five of 14 genes with higher transcript levels in DpDf tissues and 11 of 16 genes with lower transcript levels in DpDf plants analyzed by qRT-PCR showed relatively mild quantitative changes in expression levels in DpDf meristems that progressed throughout leaf development and resulted in qualitative (absence/presence) variation and ectopic gene expression or erroneous transcriptional silencing of these genes in mature leaf tissues (Fig. 4). For example, in wild-type plants, gene BM379473 was expressed in meristem tissues and displayed progressively lower transcript levels during leaf development, so that it was not expressed at all in mature leaves. This gene was expressed at a 2-fold higher level in DpDf meristems and remained ectopically expressed at meristem tissue level in DpDf mature leaf tissues. Similar examples of progressive alteration of gene expression patterns were discovered during early seedling development right after germination (Table IV ). We found that at least six out of nine genes with higher transcript levels in DpDf tissues and four out of six genes with lower transcript levels in DpDf tissues showed a pattern of progressive alteration of gene expression patterns during early development, when gene expression levels were tested in seedlings at three time points after germination. In these examples, 5-mm DpDf seedlings did not show significant changes in expression relative to wild-type seedlings, while DpDf seedlings at later time points during development (15- or 30-mm seedlings) demonstrated significant quantitative or qualitative changes in gene expression levels (Table IV). Although we could not directly link changes in the expression of a particular gene to a particular phenotypic alteration of DpDf plants, taken together our data strongly suggested that tissue-specific qualitative changes in gene expression patterns are a common response to aneuploidy in plants and are a likely cause of phenotypic alterations seen in DpDf plants.
The concept of "gene dosage balance" has always been viewed as an important mechanism for the regulation of gene function. It has been long known that aneuploidy causes different phenotypic abnormalities both in plants and animals. Interestingly, despite the existence of similar phenotypic defects in different aneuploids (Torres et al., 2007 ), many such phenotypic abnormalities are frequently specific depending on the particular chromosome that is being duplicated. For example, in human, trisomy 13 (Patau syndrome), 18 (Edwards syndrome), 21 (Down syndrome), and trisomy of sex chromosomes (Kleinfelter syndrome) have different phenotypic characteristics and different degrees of mental retardation and lethality (for review, see Altug-Teber et al., 2007). Trisomic Datura lines differ in the structure of the leaves and flowers (Blakeslee et al., 1920). Trisomic maize plants possess characteristic features in leaf and tassel structure, plant architecture, developmental stages, and other characteristics (www.maizegdb.org). Such differences suggest that there might be specific "key" genes on each of the chromosomes that cause these phenotypic effects when their copy number is out of balance with other genes. However, even with a small duplicated regions (chromosome 21 in Down syndrome contains only 350 genes), it is still impossible to create genetic models for each of the duplicated genes to identify such key genes responsible for specific phenotypes (Laffaire et al., 2009). Therefore, most of the recent investigations of effects of aneuploidy have been focused on global gene expression profiling. However, plant studies of tissue-specific effects of aneuploidy on gene expression have been limited to several genes and a limited number of tissues (Guo and Birchler, 1994; Cooper and Birchler, 2001). Tissue-specific aneuploidy effects on global gene expression have not been studied in plants.
We performed global gene expression profiling in meristem-enriched and predominantly leaf maize seedling tissues and found that these two tissues differed substantially in the prevalence of trans- and cis-effects and dosage compensation. Interestingly, meristem-enriched tissues showed a much higher rate of trans-effects and a much stronger level of dosage compensation for the trisomic genes. The results of more detailed study of 30 genes in six tissues of DpDf and wild-type maize seedlings and at three early developmental points further suggest that effects of aneuploidy on global gene expression depend on the tissue analyzed. In a study of mouse cerebellar regions enriched in granule cell precursors, similar differences in primary (cis) and secondary (trans) effects of trisomy have been observed between cells at different developmental stages and likely even between different cells of the same stage (Laffaire et al., 2009
Stress-response genes, transcription factors, and other potential regulatory genes have been frequently reported to be overrepresented among the genes affected by aneuploidy both in plants and animals (Huettel et al., 2008
It is commonly assumed that phenotypic alterations caused by aneuploids occur due to quantitative changes in the expression of genes with altered dosage (cis-effects) or genes located throughout the genome (trans-effects). It has been previously reported that aneuploidy causes greater quantitative changes in gene expression of two maize genes (sus1 and sh1) in 2-week-old plants compared with embryo and endosperm tissues (Cooper and Birchler, 2001 Based on the results of global gene expression profiling of aneuploids, it could be concluded that regardless of the system analyzed, aneuploidy causes (1) cis-effects, correlated to gene dosage and predominantly common to multiple tissues; (2) varying degrees of gene dosage compensation for trisomic genes; (3) tissue-specific trans-effects (likely as a result of misregulation due to the slight variation in the presence of a regulatory protein); and (4) tissue-specific fixed qualitative variation in gene expression patterns that is more frequent in mature tissues. However, all of these changes have been reported for gene mRNA levels, and it is still unclear which of these effects are translated to the protein level and are indeed important for phenotypic abnormalities, given the existence of posttranscriptional and posttranslational regulation mechanisms.
Plant Materials and Tissue Collection
Maize (Zea mays) stocks carrying a T5-6b translocation, backcrossed into the B73 genetic background for over 10 generations, were obtained from the University of Minnesota collection. The interchange T5-6b carries a break at 5S.1 (approximately 350 centimorgan on IBM2 2008 Neighbors genetic map; in contig 238 according to www.maizesequence.org) and between the middle and distal chromomere satellites of 6S (break occurs prior to 70 centimorgan on IBM2 2008 Neighbors genetic map; in contig 257 according to www.maizesequence.org; Phillips, 1969
RNA isolation and Affymetrix microarray hybridization were performed as described (Makarevitch et al., 2008
We combined the microarray data from meristem-enriched tissue (described in this experiment; National Center for Biotechnology Information Gene Expression Omnibus Series submission no. GSE19212) and the data from total seedlings (National Center for Biotechnology Information Gene Expression Omnibus Series submission no. GSE10243; Makarevitch et al., 2008 To address the possibility that average gene expression could differ between wild-type and aneuploid tissues, thus skewing results of the analysis in case of normalization between the arrays, alternative analyses were performed. In the first approach, normalization between arrays was performed, while leaving out the genes located on either all chromosomes 5 and 6 or in aneuploid regions of chromosomes 5 and 6 and expected to be differentially expressed in aneuploid tissues. In the second approach, microarray data were normalized using per gene normalization and genes differentially expressed in DpDf plants relative to wild-type siblings were identified by one-way ANOVA on the GC-RMA values using a nonparametric test. Despite some differences in the number of genes identified as differentially expressed, all of these analyses yielded similar results concerning the frequency and distribution of trans- and cis-effects between two tissues. To confirm that applying the cutoff to filter genes with very small changes in gene expression does not alter the results of our analysis, the cutoff requirement was dropped and genes with significant changes in expression levels in DpDf versus wild-type tissues were identified using a false-discovery rate significance threshold of 0.05 and an expression level threshold of at least 50 units for GC-RMA values in at least one of the genotypes. In this analysis, the number of differentially expressed genes increased to 1,846. However, the proportion and distribution of trans- and cis-effects between different tissues remained very similar (for details of this additional analysis, see Supplemental Tables S2 and S3; Supplemental Text S1).
One microgram of total RNA was treated with DNase I (Qiagen) and used for cDNA synthesis using Qiagen Omniscript reverse transcriptase (Qiagen) according to the manufacturer's instructions and was diluted 1:5 for use in qRT-PCR experiments. Thirty genes that exhibited variation in expression patterns between DpDf and wild-type genotypes in meristem-enriched and/or whole seedling tissues were selected for qRT-PCR analysis. Primers for these 30 genes (Supplemental Table S5) and three control genes (actin1, gene identifier 100282267; GAPC, 542367; and mez1, 541954) were designed using Primer 3.0 software (Rozen and Skaletsky, 2000
Annotations for differentially expressed genes were based on information available at The Institute for Genomic Research Maize Gene Index (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=maize). The Gene Ontology annotations were obtained based on the assignment of the best Arabidopsis (Arabidopsis thaliana) hits from The Arabidopsis Information Resource Web site (http://www.arabidopsis.org/tools/bulk/go/index.jsp) and based on microarray annotations provided by Affymetrix. The genetic map positions for Affymetrix array probe sets were predicted based on identity with genetically mapped sequences or inferred based upon identity with bacterial artificial chromosome contig sequences that contained genetically mapped markers (www.maizesequence.org, www.maizegdb.org, http://www.genome.arizona.edu).
The following materials are available in the online version of this article.
We gratefully acknowledge the assistance of Peter Hermanson and Nathan Springer in helping with plant growth and plant crosses. We are greatly thankful to Nathan Springer, who suggested an idea for this project, provided genetic stocks and field space, and patiently offered helpful comments at multiple stages of this project, including writing and editing of the manuscript. We appreciate the suggestions of three anonymous reviewers that greatly strengthened the manuscript. Received November 4, 2009; accepted December 8, 2009; published December 11, 2009.
1 This work was supported by the Hamline Biology Lund Fund and the Fairchild Sherman Foundation.  The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Irina Makarevitch (imakarevitch01{at}hamline.edu).
[W] The online version of this article contains Web-only data.
[OA] Open Access articles can be viewed online without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.109.150466 * Corresponding author; e-mail imakarevitch01{at}hamline.edu.
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TOP
ABSTRACT
RESULTS
Ct value (difference in the number of cycles to reach a threshold) was calculated by subtracting the Ct mean value for the samples to be compared (DpDf versus the wild type). Fold differences for a given primer combination were calculated as (primer efficiency)