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Plant Physiol, July 2000, Vol. 123, pp. 795-806

Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You1

Wolfgang Lukowitz,* C. Stewart Gillmor, and Wolf-Rüdiger Scheible

Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305 (W.L., C.S.G., W.-R.S.); and Department of Biological Sciences, Stanford University, Gilbert Hall, Stanford, California 94305 (C.S.G.)

Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.


1 W.L. was partially supported by a postdoctoral fellowship (LT 594/96) from the Human Frontier Science Program Organization and by a fellowship from the Carnegie Institution of Washington. C.S.G. was supported by a U.S. Department of Energy/National Science Foundation/U.S. Department of Agriculture TRI-Agency Training Grant, and W.-R.S. was supported by a postdoctoral fellowship (Sche 548 1/1) from the Deutsche Forschungsgemeinschaft.

* Corresponding author; e-mail lubo{at}andrew2.stanford.edu; fax 650-325-6857.

© 2000 American Society of Plant Physiologists



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Plant Physiol.Home page
D. Eapen, M. L. Barroso, M. E. Campos, G. Ponce, G. Corkidi, J. G. Dubrovsky, and G. I. Cassab
A no hydrotropic response Root Mutant that Responds Positively to Gravitropism in Arabidopsis
Plant Physiology, February 1, 2003; 131(2): 536 - 546.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
O. Loudet, S. Chaillou, P. Merigout, J. Talbotec, and F. Daniel-Vedele
Quantitative Trait Loci Analysis of Nitrogen Use Efficiency in Arabidopsis
Plant Physiology, January 1, 2003; 131(1): 345 - 358.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
C. S. Chan, H.-P. Peng, and M.-C. Shih
Mutations Affecting Light Regulation of Nuclear Genes Encoding Chloroplast Glyceraldehyde-3-Phosphate Dehydrogenase in Arabidopsis
Plant Physiology, November 1, 2002; 130(3): 1476 - 1486.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
M. Gonzalez-Guzman, N. Apostolova, J. M. Belles, J. M. Barrero, P. Piqueras, M. R. Ponce, J. L. Micol, R. Serrano, and P. L. Rodriguez
The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde
PLANT CELL, August 1, 2002; 14(8): 1833 - 1846.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
G. Jander, S. R. Norris, S. D. Rounsley, D. F. Bush, I. M. Levin, and R. L. Last
Arabidopsis Map-Based Cloning in the Post-Genome Era
Plant Physiology, June 1, 2002; 129(2): 440 - 450.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
R. Sollner, G. Glasser, G. Wanner, C. R. Somerville, G. Jurgens, and F. F. Assaad
Cytokinesis-Defective Mutants of Arabidopsis
Plant Physiology, June 1, 2002; 129(2): 678 - 690.
[Abstract] [Full Text] [PDF]


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JCBHome page
C. S. Gillmor, P. Poindexter, J. Lorieau, M. M. Palcic, and C. Somerville
{alpha}-Glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis
J. Cell Biol., March 18, 2002; 156(6): 1003 - 1013.
[Abstract] [Full Text] [PDF]


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GeneticsHome page
R. T. Durrett, K.-Y. Chen, and S. D. Tanksley
A Simple Formula Useful for Positional Cloning
Genetics, January 1, 2002; 160(1): 353 - 355.
[Abstract] [Full Text] [PDF]


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GeneticsHome page
J. McElver, I. Tzafrir, G. Aux, R. Rogers, C. Ashby, K. Smith, C. Thomas, A. Schetter, Q. Zhou, M. A. Cushman, et al.
Insertional Mutagenesis of Genes Required for Seed Development in Arabidopsis thaliana
Genetics, December 1, 2001; 159(4): 1751 - 1763.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
J. L. Peters, H. Constandt, P. Neyt, G. Cnops, J. Zethof, M. Zabeau, and T. Gerats
A Physical Amplified Fragment-Length Polymorphism Map of Arabidopsis
Plant Physiology, December 1, 2001; 127(4): 1579 - 1589.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
A. E. Pepper, M.-s. Seong-Kim, S. M. Hebst, K. N. Ivey, S.-J. Kwak, and D. E. Broyles
shl, a New Set of Arabidopsis Mutants with Exaggerated Developmental Responses to Available Red, Far-Red, and Blue Light
Plant Physiology, September 1, 2001; 127(1): 295 - 304.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
W.-R. Scheible, R. Eshed, T. Richmond, D. Delmer, and C. Somerville
Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants
PNAS, August 17, 2001; (2001) 191361598.
[Abstract] [Full Text] [PDF]


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GeneticsHome page
I. W. Wilson, C. L. Schiff, D. E. Hughes, and S. C. Somerville
Quantitative Trait Loci Analysis of Powdery Mildew Disease Resistance in the Arabidopsis thaliana Accession Kashmir-1
Genetics, July 1, 2001; 158(3): 1301 - 1309.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
W. Lukowitz, T. C. Nickle, D. W. Meinke, R. L. Last, P. L. Conklin, and C. R. Somerville
Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis
PNAS, February 15, 2001; (2001) 51625798.
[Abstract] [Full Text]


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Plant Physiol.Home page
E. Drenkard, B. G. Richter, S. Rozen, L. M. Stutius, N. A. Angell, M. Mindrinos, R. J. Cho, P. J. Oefner, R. W. Davis, and F. M. Ausubel
A Simple Procedure for the Analysis of Single Nucleotide Polymorphisms Facilitates Map-Based Cloning in Arabidopsis
Plant Physiology, December 1, 2000; 124(4): 1483 - 1492.
[Abstract] [Full Text]


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Plant Physiol.Home page
M. E. Nasrallah, K. Yogeeswaran, S. Snyder, and J. B. Nasrallah
Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants
Plant Physiology, December 1, 2000; 124(4): 1605 - 1614.
[Abstract] [Full Text]


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Proc. Natl. Acad. Sci. USAHome page
W. Lukowitz, T. C. Nickle, D. W. Meinke, R. L. Last, P. L. Conklin, and C. R. Somerville
Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis
PNAS, February 27, 2001; 98(5): 2262 - 2267.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
W.-R. Scheible, R. Eshed, T. Richmond, D. Delmer, and C. Somerville
Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants
PNAS, August 28, 2001; 98(18): 10079 - 10084.
[Abstract] [Full Text] [PDF]




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