First published online May 2, 2002; 10.1104/pp.002089
Plant Physiol, June 2002, Vol. 129, pp. 616-624
Single and Double Knockouts of the Genes for Photosystem I
Subunits G, K, and H of Arabidopsis. Effects on Photosystem I
Composition, Photosynthetic Electron Flow, and State
Transitions1
Claudio
Varotto,
Paolo
Pesaresi,
Peter
Jahns,
Angela
Leßnick,
Marco
Tizzano,
Fabio
Schiavon,
Francesco
Salamini, and
Dario
Leister*
Zentrum zur Identifikation von Genfunktionen durch
Insertionsmutagenese bei Arabidopsis thaliana (C.V.,
P.P., A.L., D.L.), and Abteilung für Pflanzenzüchtung und
Ertragsphysiologie, Max-Planck-Institut für
Züchtungsforschung, Carl-von-Linné Weg 10, 50829 Köln, Germany (M.T., F.Sc., F.Sa., D.L.); and Institut für
Biochemie der Pflanzen, Heinrich-Heine-Universität
Düsseldorf, Universitätsstra e 1, 40225 Düsseldorf,
Germany (P.J.)
Photosystem I (PSI) of higher plants contains 18 subunits.
Using Arabidopsis En insertion lines, we have isolated
knockout alleles of the genes psaG,
psaH2, and psaK, which code for PSI-G, -H, and -K. In the mutants psak-1 and
psag-1.4, complete loss of PSI-K and -G, respectively,
was confirmed, whereas the residual H level in psah2-1.4
is due to a second gene encoding PSI-H, psaH1. Double
mutants, lacking PSI-G, and also -K, or a fraction of -H, together with
the three single mutants were characterized for their growth phenotypes
and PSI polypeptide composition. In general, the loss of each subunit
has secondary, in some cases additive, effects on the abundance of
other PSI polypeptides, such as D, E, H, L, N, and the light-harvesting
complex I proteins Lhca2 and 3. In the G-less mutant
psag-1.4, the variation in PSI composition suggests that
PSI-G stabilizes the PSI-core. Levels of light-harvesting complex I
proteins in plants, which lack simultaneously PSI-G and -K, indicate
that PSI subunits other than G and K can also bind Lhca2 and 3. In the
same single and double mutants, psag-1.4, psak-1, psah2-1.4,
psag-1.4/psah2-1.4, and
psag-1.4/psak-1 photosynthetic electron
flow and excitation energy quenching were analyzed to address the roles
of the various subunits in P700 reduction (mediated by PSI-F and -N)
and oxidation (PSI-E), and state transitions (PSI-H). Based on the
results, we also suggest for PSI-K a role in state transitions.
1
This work was supported by the Deutsche
Forschungsgemeinschaft (grant nos. Ja 665/2-1 to P.J. and Le
1265/1-1, 2 to D.L.) and by the Bundesministerium für Bildung
und Forschung (to D.L.).
*
Corresponding author; e-mail leister{at}mpiz-koeln.mpg.de; fax
49-221-5062-413.
© 2002 American Society of Plant Physiologists
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