First published online May 2, 2002; 10.1104/pp.001362
Plant Physiol, June 2002, Vol. 129, pp. 838-853
Heat Stress- and Heat Shock Transcription Factor-Dependent
Expression and Activity of Ascorbate Peroxidase in
Arabidopsis1
Irina I.
Panchuk,
Roman A.
Volkov, and
Friedrich
Schöffl*
Zentrum für Molekularbiologie der Pflanzen (Center of Plant
Molecular Biology), Allgemeine Genetik, Universität
Tübingen, 72076 Tübingen, Germany
To find evidence for a connection between heat stress
response, oxidative stress, and common stress tolerance, we studied the
effects of elevated growth temperatures and heat stress on the activity
and expression of ascorbate peroxidase (APX). We compared wild-type
Arabidopsis with transgenic plants overexpressing heat shock
transcription factor 3 (HSF3), which synthesize heat shock proteins and
are improved in basal thermotolerance. Following heat stress, APX
activity was positively affected in transgenic plants and correlated
with a new thermostable isoform, APXS. This enzyme was
present in addition to thermolabile cytosolic APX1, the prevalent
isoform in unstressed cells. In HSF3-transgenic plants,
APXS activity was detectable at normal temperature and
persisted after severe heat stress at 44°C. In nontransgenic plants,
APXS was undetectable at normal temperature, but could be
induced by moderate heat stress. The mRNA expression profiles of known and three new Apx genes were determined using real-time
PCR. Apx1 and Apx2 genes encoding
cytosolic APX were heat stress and HSF dependently expressed, but only
the representations of Apx2 mRNA met the criteria that
suggest identity between APXS and APX2: not expressed at
normal temperature in wild type, strong induction by heat stress, and
HSF3-dependent expression in transgenic plants. Our data suggest that
Apx2 is a novel heat shock gene and that the enzymatic
activity of APX2/APXS is required to compensate heat
stress-dependent decline of APX1 activity in the cytosol. The
functional roles of modulations of APX expression and the
interdependence of heat stress and oxidative stress response and
signaling mechanisms are discussed.
1
This work was supported in part by
Sonderforschungsbereich 446 University of Tübingen of the
Deutsche Forschungsgemeinschaft.
*
Corresponding author; e-mail
friedrich.schoeffl{at}zmbp.uni-tuebingen.de; fax
49-7071-29-5042.
© 2002 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
I. Granlund, P. Storm, M. Schubert, J. G. Garcia-Cerdan, C. Funk, and W. P. Schroder
The TL29 Protein is Lumen Located, Associated with PSII and Not an Ascorbate Peroxidase
Plant Cell Physiol.,
November 1, 2009;
50(11):
1898 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. J. Im, M. Ji, A. Lee, R. Killens, A. M. Grunden, and W. F. Boss
Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance
Plant Physiology,
October 1, 2009;
151(2):
893 - 904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Frank, E. Pressman, R. Ophir, L. Althan, R. Shaked, M. Freedman, S. Shen, and N. Firon
Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response
J. Exp. Bot.,
September 1, 2009;
60(13):
3891 - 3908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zhang, Y. Li, D. Xing, and C. Gao
Characterization of mitochondrial dynamics and subcellular localization of ROS reveal that HsfA2 alleviates oxidative damage caused by heat stress in Arabidopsis
J. Exp. Bot.,
May 1, 2009;
60(7):
2073 - 2091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Koussevitzky, N. Suzuki, S. Huntington, L. Armijo, W. Sha, D. Cortes, V. Shulaev, and R. Mittler
Ascorbate Peroxidase 1 Plays a Key Role in the Response of Arabidopsis thaliana to Stress Combination
J. Biol. Chem.,
December 5, 2008;
283(49):
34197 - 34203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Muhlenbock, M. Szechynska-Hebda, M. Plaszczyca, M. Baudo, A. Mateo, P. M. Mullineaux, J. E. Parker, B. Karpinska, and S. Karpinski
Chloroplast Signaling and LESION SIMULATING DISEASE1 Regulate Crosstalk between Light Acclimation and Immunity in Arabidopsis
PLANT CELL,
September 1, 2008;
20(9):
2339 - 2356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, S. Bajad, J. Shuman, V. Shulaev, and R. Mittler
The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana
J. Biol. Chem.,
April 4, 2008;
283(14):
9269 - 9275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Bechtold, O. Richard, A. Zamboni, C. Gapper, M. Geisler, B. Pogson, S. Karpinski, and P. M. Mullineaux
Impact of chloroplastic- and extracellular-sourced ROS on high light-responsive gene expression in Arabidopsis
J. Exp. Bot.,
February 1, 2008;
59(2):
121 - 133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Rossel, P. B. Wilson, D. Hussain, N. S. Woo, M. J. Gordon, O. P. Mewett, K. A. Howell, J. Whelan, K. Kazan, and B. J. Pogson
Systemic and Intracellular Responses to Photooxidative Stress in Arabidopsis
PLANT CELL,
December 1, 2007;
19(12):
4091 - 4110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ogawa, K. Yamaguchi, and T. Nishiuchi
High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth
J. Exp. Bot.,
September 20, 2007;
(2007)
erm184v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yamasaki, S. E. Abdel-Ghany, C. M. Cohu, Y. Kobayashi, T. Shikanai, and M. Pilon
Regulation of Copper Homeostasis by Micro-RNA in Arabidopsis
J. Biol. Chem.,
June 1, 2007;
282(22):
16369 - 16378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kotak, E. Vierling, H. Baumlein, and P. v. Koskull-Doring
A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis
PLANT CELL,
January 1, 2007;
19(1):
182 - 195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-y. Charng, H.-c. Liu, N.-y. Liu, W.-t. Chi, C.-n. Wang, S.-h. Chang, and T.-t. Wang
A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in Arabidopsis
Plant Physiology,
January 1, 2007;
143(1):
251 - 262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Y. Yoo, K. Miura, J. B. Jin, J. Lee, H. C. Park, D. E. Salt, D.-J. Yun, R. A. Bressan, and P. M. Hasegawa
SIZ1 Small Ubiquitin-Like Modifier E3 Ligase Facilitates Basal Thermotolerance in Arabidopsis Independent of Salicylic Acid
Plant Physiology,
December 1, 2006;
142(4):
1548 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. F. XU and W. M. SHI
Expression Profiling of the 14-3-3 Gene Family in Response to Salt Stress and Potassium and Iron Deficiencies in Young Tomato (Solanum lycopersicum) Roots: Analysis by Real-time RT-PCR
Ann. Bot.,
November 1, 2006;
98(5):
965 - 974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Narendra, S. Venkataramani, G. Shen, J. Wang, V. Pasapula, Y. Lin, D. Kornyeyev, A. S. Holaday, and H. Zhang
The Arabidopsis ascorbate peroxidase 3 is a peroxisomal membrane-bound antioxidant enzyme and is dispensable for Arabidopsis growth and development
J. Exp. Bot.,
September 1, 2006;
57(12):
3033 - 3042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. MILLER and R. MITTLER
Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?
Ann. Bot.,
August 1, 2006;
98(2):
279 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kojo, T. Yaeno, K. Kusumi, H. Matsumura, S. Fujisawa, R. Terauchi, and K. Iba
Regulatory Mechanisms of ROI Generation are Affected by Rice spl Mutations
Plant Cell Physiol.,
August 1, 2006;
47(8):
1035 - 1044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Baier and K.-J. Dietz
Chloroplasts as source and target of cellular redox regulation: a discussion on chloroplast redox signals in the context of plant physiology
J. Exp. Bot.,
June 1, 2005;
56(416):
1449 - 1462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald
Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway
Plant Physiology,
June 1, 2005;
138(2):
686 - 700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Davletova, L. Rizhsky, H. Liang, Z. Shengqiang, D. J. Oliver, J. Coutu, V. Shulaev, K. Schlauch, and R. Mittler
Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactive Oxygen Gene Network of Arabidopsis
PLANT CELL,
January 1, 2005;
17(1):
268 - 281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Panikulangara, G. Eggers-Schumacher, M. Wunderlich, H. Stransky, and F. Schoffl
Galactinol synthase1. A Novel Heat Shock Factor Target Gene Responsible for Heat-Induced Synthesis of Raffinose Family Oligosaccharides in Arabidopsis
Plant Physiology,
October 1, 2004;
136(2):
3148 - 3158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Gachon, A. Mingam, and B. Charrier
Real-time PCR: what relevance to plant studies?
J. Exp. Bot.,
July 1, 2004;
55(402):
1445 - 1454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rizhsky, H. Liang, J. Shuman, V. Shulaev, S. Davletova, and R. Mittler
When Defense Pathways Collide. The Response of Arabidopsis to a Combination of Drought and Heat Stress
Plant Physiology,
April 1, 2004;
134(4):
1683 - 1696.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rizhsky, S. Davletova, H. Liang, and R. Mittler
The Zinc Finger Protein Zat12 Is Required for Cytosolic Ascorbate Peroxidase 1 Expression during Oxidative Stress in Arabidopsis
J. Biol. Chem.,
March 19, 2004;
279(12):
11736 - 11743.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Fourcroy, G. Vansuyt, S. Kushnir, D. Inze, and J.-F. Briat
Iron-Regulated Expression of a Cytosolic Ascorbate Peroxidase Encoded by the APX1 Gene in Arabidopsis Seedlings
Plant Physiology,
February 1, 2004;
134(2):
605 - 613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Wolucka and M. Van Montagu
GDP-Mannose 3',5'-Epimerase Forms GDP-L-gulose, a Putative Intermediate for the de Novo Biosynthesis of Vitamin C in Plants
J. Biol. Chem.,
November 28, 2003;
278(48):
47483 - 47490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Volkov, I. I. Panchuk, and F. Schoffl
Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR
J. Exp. Bot.,
October 1, 2003;
54(391):
2343 - 2349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-T. Liu, B. Li, Z.-L. Shang, X.-Z. Li, R.-L. Mu, D.-Y. Sun, and R.-G. Zhou
Calmodulin Is Involved in Heat Shock Signal Transduction in Wheat
Plant Physiology,
July 1, 2003;
132(3):
1186 - 1195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Rossel, I. W. Wilson, and B. J. Pogson
Global Changes in Gene Expression in Response to High Light in Arabidopsis
Plant Physiology,
November 1, 2002;
130(3):
1109 - 1120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
