First published online June 14, 2002; 10.1104/pp.001677
Plant Physiol, July 2002, Vol. 129, pp. 1150-1159
PROLIFERATING INFLORESCENCE MERISTEM, a MADS-Box Gene
That Regulates Floral Meristem Identity in Pea1
Scott A.
Taylor,2
Julie M.I.
Hofer,*
Ian C.
Murfet,
John D.
Sollinger,3
Susan R.
Singer,
Maggie R.
Knox, and
T.H. Noel
Ellis
School of Plant Science, University of Tasmania, G.P.O. Box
252-55, Hobart, Tasmania, 7001, Australia (S.A.T., I.C.M.); John Innes
Centre, Colney Lane, Norwich NR4 7UH, United Kingdom (S.A.T., J.M.I.H.,
M.R.K., T.H.N.E.); and Department of Biology, Carleton College,
Northfield, Minnesota 55057 (J.D.S., S.R.S.)
SQUAMOSA and APETALA1 are
floral meristem identity genes from snapdragon
(Antirrhinum majus) and Arabidopsis,
respectively. Here, we characterize the floral meristem identity
mutation proliferating inflorescence meristem
(pim) from pea (Pisum sativum) and show that it corresponds to a defect in the PEAM4 gene, a
homolog of SQUAMOSA and APETALA1. The
PEAM4 coding region was deleted in the
pim-1 allele, and this deletion cosegregated with the
pim-1 mutant phenotype. The pim-2 allele
carried a nucleotide substitution at a predicted 5' splice site that
resulted in mis-splicing of pim-2 mRNA. PCR products
corresponding to unspliced and exon-skipped mRNA species were observed.
The pim-1 and pim-2 mutations delayed floral meristem specification and altered floral morphology
significantly but had no observable effect on vegetative development.
These floral-specific mutant phenotypes and the restriction of
PIM gene expression to flowers contrast with other known
floral meristem genes in pea that additionally affect vegetative
development. The identification of PIM provides an
opportunity to compare pathways to flowering in species with different
inflorescence architectures.
1
This work was supported by the Australian
Research Council (I.C.M.), the Biotechnology and Biological Sciences
Research Council, UK (T.H.N.E. and M.R.K.), the Department for
Environment, Food, and Rural Affairs, UK (grant no. AR0102 to
J.M.I.H.), and the National Science Foundation (grant no. NSF 9977087 to S.R.S.). S.A.T. received funding from an Australian Postgraduate
Award and European Union project EuDicot Map (no. B104 CT 97-2170).
2
Present address: John Innes Centre, Norwich NR4 7UH, UK.
3
Present address: Southern Oregon University, Ashland, OR
97520-5071.
*
Corresponding author; e-mail julie.hofer{at}bbsrc.ac.uk; fax
44-1603-450045.
© 2002 American Society of Plant Physiologists
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