First published online July 18, 2002; 10.1104/pp.006072
Plant Physiol, August 2002, Vol. 129, pp. 1880-1891
Two Novel Mitogen-Activated Protein Signaling Components, OsMEK1
and OsMAP1, Are Involved in a Moderate Low-Temperature Signaling
Pathway in Rice1
Jiang-Qi
Wen,2
Kiyoharu
Oono,3 and
Ryozo
Imai*
Winter Stress Laboratory, National Agricultural Research Center for
Hokkaido Region, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555,
Japan
Rice (Oryza sativa) anther development is easily
damaged by moderately low temperatures above 12°C. Subtractive
screening of cDNA that accumulated in 12°C-treated anthers identified
a cDNA clone, OsMEK1, encoding a protein with features
characteristic of a mitogen-activated protein (MAP) kinase kinase. The
putative OsMEK1 protein shows 92% identity to the maize (Zea
mays) MEK homolog, ZmMEK1. OsMEK1
transcript levels were induced in rice anthers by 12°C treatment for
48 h. Similar OsMEK1 induction was observed in
shoots and roots of seedlings that were treated at 12°C for up to
24 h. It is interesting that no induction of OsMEK1 transcripts was observed in 4°C-treated seedlings. In contrast, rice
lip19, encoding a bZIP protein possibly involved in low
temperature signal transduction, was not induced by 12°C treatment
but was induced by 4°C treatment. Among the three MAP kinase homologs cloned, only OsMAP1 displayed similar 12°C-specific
induction pattern as OsMEK1. A yeast two-hybrid system
revealed that OsMEK1 interacts with OsMAP1, but not with OsMAP2 and
OsMAP3, suggesting that OsMEK1 and OsMAP1 probably function in the same
signaling pathway. An in-gel assay of protein kinase activity revealed
that a protein kinase (approximately 43 kD), which preferentially uses myelin basic protein as a substrate, was activated by 12°C treatment but not by 4°C treatment. Taken together, these results lead us to
conclude that at least two signaling pathways for low temperature stress exist in rice, and that a MAP kinase pathway with OsMEK1 and
OsMAP1 components is possibly involved in the signaling for the higher
range low-temperature stress.
1
This work was supported by the Special
Coordination Funds of the Science and Technology Agency of Japanese
Government and the Cooperative System for Supporting Priority Research
from Japan Science and Technology Cooperation (to R.I.). J.-Q.W. was
supported by a Science and Technology Agency Fellowship from the
Science and Technology Agency of Japan.
2
Present address: Division of Biological Sciences,
University of Missouri, Columbia, MO 65211.
3
Present address: Department of Biotechnology, National
Institute for Agrobiological Sciences, Kannondai, Tsukuba, Japan.
*
Corresponding author; e-mail rzi{at}affrc.go.jp; fax
81-11-857-9382.
© 2002 American Society of Plant Physiologists
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