First published online September 20, 2002; 10.1104/pp.007781
Plant Physiol, October 2002, Vol. 130, pp. 784-795
Inventory and Functional Characterization of the HAK Potassium
Transporters of Rice1
María A.
Bañuelos,
Blanca
Garciadeblas,
Beatriz
Cubero, and
Alonso
Rodríguez-Navarro*
Departamento de Biotecnología, Universidad
Politécnica de Madrid, 28040 Madrid, Spain (M.A.B., B.G.,
A.R.-N.); and Instituto de Recursos Naturales y Agrobiología,
Consejo Superior de Investigaciones Científicas, 41080 Sevilla,
Spain (B.C.)
Plants take up large amounts of K+ from the soil
solution and distribute it to the cells of all organs, where it
fulfills important physiological functions. Transport of K+
from the soil solution to its final destination is mediated by channels
and transporters. To better understand K+ movements in
plants, we intended to characterize the function of the large
KT-HAK-KUP family of transporters in rice (Oryza sativa
cv Nipponbare). By searching in databases and cDNA cloning, we have
identified 17 genes (OsHAK1-17) encoding
transporters of this family and obtained evidence of the existence of
other two genes. Phylogenetic analysis of the encoded transporters
reveals a great diversity among them, and three distant transporters, OsHAK1, OsHAK7, and OsHAK10, were expressed in yeast
(Saccharomyces cerevisiae) and bacterial mutants to
determine their functions. The three transporters mediate
K+ influxes or effluxes, depending on the conditions of the
experiment. A comparative kinetic analysis of HAK-mediated
K+ influx in yeast and in roots of K+-starved
rice seedlings demonstrated the involvement of HAK transporters in root
K+ uptake. We discuss that all HAK transporters may mediate
K+ transport, but probably not only in the plasma membrane.
Transient expression of the OsHAK10-green fluorescent protein
fusion protein in living onion epidermal cells targeted this
protein to the tonoplast.
1
This work was supported by the
Consejería de Educación y Cultura de la Comunidad de
Madrid (Programa de Grupos Estratégicos) and by the Ministerio de
Ciencia y Tecnología (grant no. BIO2000-0938).
*
Corresponding author; e-mail arodrignavar{at}bit.etsia.upm.es; fax
34-913365757.
© 2002 American Society of Plant Physiologists
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