First published online November 14, 2002; 10.1104/pp.010546
Plant Physiol, December 2002, Vol. 130, pp. 1908-1917
Auxin and Ethylene Response Interactions during Arabidopsis Root
Hair Development Dissected by Auxin Influx Modulators
Abidur
Rahman,1
Satoko
Hosokawa,
Yutaka
Oono,
Taisaku
Amakawa,
Nobuharu
Goto, and
Seiji
Tsurumi*
Graduate School of Science and Technology (A.R., T.A.), Faculty of
Science (S.H.), and Radioisotope Research Center (S.T.), Kobe
University, Rokkodai, Nada-Ku, Kobe 657-8501, Japan; Plant Resources
Laboratory, Japan Atomic Energy Research Institute, Watanuki, Takasaki
370-1292, Japan (Y.O.); and Department of Biology, Miyagi University
of Education, Aoba-Ku, Sendai 980-0845, Japan (N.G.)
The plant hormones auxin and ethylene have been shown to
play important roles during root hair development. However, cross talk
between auxin and ethylene makes it difficult to understand the
independent role of either hormone. To dissect their respective roles,
we examined the effects of two compounds, chromosaponin I (CSI) and
1-naphthoxyacetic acid (1-NOA), on the root hair developmental process
in wild-type Arabidopsis, ethylene-insensitive mutant ein2-1, and auxin influx mutants aux1-7,
aux1-22, and double mutant aux1-7 ein2.
 -Glucuronidase (GUS) expression analysis in the BA-GUS transgenic
line, consisting of auxin-responsive domains of
PS-IAA4/5 promoter and GUS
reporter, revealed that 1-NOA and CSI act as auxin uptake inhibitors in
Arabidopsis roots. The frequency of root hairs in ein2-1
roots was greatly reduced in the presence of CSI or 1-NOA, suggesting
that endogenous auxin plays a critical role for the root hair
initiation in the absence of an ethylene response. All of these mutants
showed a reduction in root hair length, however, the root hair length
could be restored with a variable concentration of 1-naphthaleneacetic
acid (NAA). NAA (10 nM) restored the root hair length of
aux1 mutants to wild-type level, whereas 100 nM NAA was needed for ein2-1 and
aux1-7 ein2 mutants. Our results suggest that
insensitivity in ethylene response affects the auxin-driven root hair
elongation. CSI exhibited a similar effect to 1-NOA, reducing root hair
growth and the number of root hair-bearing cells in wild-type and
ein2-1 roots, while stimulating these traits in
aux1-7and aux1-7ein2 roots, confirming that CSI is a unique modulator of AUX1.
1
Present address: Plant Resources Laboratory,
Japan Atomic Energy Research Institute, Watanuki, Takasaki, Gunma
370-1292, Japan.
*
Corresponding author; e-mail tsurumis{at}scitec.kobe-u.ac.jp; fax
81-78-803-5989.
© 2002 American Society of Plant Biologists
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