First published online January 2, 2003; 10.1104/pp.012963
Plant Physiol, January 2003, Vol. 131, pp. 335-344
The Pea Gene NA Encodes
ent-Kaurenoic Acid Oxidase1
Sandra E.
Davidson,
Robert
C.
Elliott,
Chris A.
Helliwell,
Andrew T.
Poole, and
James B.
Reid*
School of Plant Science, University of Tasmania, G.P.O. Box
252-55, Hobart, Tasmania, 7001, Australia (S.E.D., R.C.E., J.B.R.);
and Commonwealth Scientific and Industrial Research
Organization, Plant Industry, G.P.O. Box 1600, Canberra, Australian
Capitol Territory 2601, Australia (C.A.H., A.T.P.)
The gibberellin (GA)-deficient dwarf na
mutant in pea (Pisum sativum) has severely reduced
internode elongation, reduced root growth, and decreased leaflet size.
However, the seeds develop normally. Two genes, PsKAO1
and PsKAO2, encoding cytochrome P450 monooxygenases of
the subfamily CYP88A were isolated. Both PsKAO1 and PsKAO2 had
ent-kaurenoic acid oxidase (KAO) activity, catalyzing the three steps of the GA biosynthetic pathway from
ent-kaurenoic acid to GA12 when expressed in
yeast (Saccharomyces cerevisiae). In addition to
the intermediates ent-7 -hydroxykaurenoic acid and
GA12-aldehyde, some additional products of the pea KAO
activity were detected, including
ent-6,7-dihydroxykaurenoic acid and 7 -hydroxykaurenolide. The NA gene encodes PsKAO1,
because in two independent mutant alleles, na-1 and
na-2, PsKAO1 had altered sequences and
the five-base deletion in PsKAO1 associated with the
na-1 allele cosegregated with the dwarf
na phenotype. PsKAO1 was expressed in the
stem, apical bud, leaf, pod, and root, organs in which GA levels have
previously been shown to be reduced in na plants.
PsKAO2 was expressed only in seeds and this may explain the normal seed development and normal GA biosynthesis in seeds of
na plants.
1
This work was supported by the Australian
Research Council (grants to J.B.R.). S.E.D. was the recipient of an
Australian Postgraduate Award.
*
Corresponding author; e-mail Jim.Reid{at}utas.edu.au; fax
61-3-6226-2698.
© 2003 American Society of Plant Biologists
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