First published online January 2, 2003; 10.1104/pp.102.010785
Plant Physiol, January 2003, Vol. 131, pp. 345-358
Quantitative Trait Loci Analysis of Nitrogen Use Efficiency in
Arabidopsis
Olivier
Loudet,*
Sylvain
Chaillou,
Patricia
Merigout,
Joël
Talbotec, and
Françoise
Daniel-Vedele
Institut National de la Recherche Agronomique, Unité de
Nutrition Azotée des Plantes, Centre de Versailles, 78 026 Versailles, France
Improving plant nitrogen (N) use efficiency or controlling soil N
requires a better knowledge of the regulation of plant N metabolism.
This could be achieved using Arabidopsis as a model genetic system,
taking advantage of the natural variation available among ecotypes.
Here, we describe an extensive study of N metabolism variation in the
Bay-0 × Shahdara recombinant inbred line population, using
quantitative trait locus (QTL) mapping. We mapped QTL for traits such
as shoot growth, total N, nitrate, and free-amino acid contents,
measured in two contrasting N environments (contrasting nitrate
availability in the soil), in controlled conditions. Genetic variation
and transgression were observed for all traits, and most of the genetic
variation was identified through QTL and QTL × QTL epistatic
interactions. The 48 significant QTL represent at least 18 loci that
are polymorphic between parents; some may correspond to known genes
from the N metabolic pathway, but others represent new genes
controlling or interacting with N physiology. The correlations between
traits are dissected through QTL colocalizations: The identification of
the individual factors contributing to the regulation of different
traits sheds new light on the relations among these characters. We also
point out that the regulation of our traits is mostly specific to the N
environment (N availability). Finally, we describe four interesting
loci at which positional cloning is feasible.
*
Corresponding author; e-mail
loudet{at}versailles.inra.fr; fax 33-1-30-83-30-96.
© 2003 American Society of Plant Biologists
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