First published online January 31, 2003; 10.1104/pp.014134
Plant Physiol, February 2003, Vol. 131, pp. 409-418
A Sequence-Based Map of Arabidopsis Genes with Mutant
Phenotypes1,[w]
David W.
Meinke,*
Laura K.
Meinke,2
Thomas C.
Showalter,3
Anna M.
Schissel,4
Lukas A.
Mueller, and
Iris
Tzafrir
Department of Botany, Oklahoma State University, Stillwater,
Oklahoma 74078 (D.W.M., L.K.M., T.C.S., A.M.S., I.T.); and Department
of Plant Biology, Carnegie Institution, 260 Panama Street, Stanford,
California 94305 (L.A.M.)
The classical genetic map of Arabidopsis contains 462 genes with
mutant phenotypes. Chromosomal locations of these genes have been
determined over the past 25 years based on recombination frequencies
with visible and molecular markers. The most recent update of the
classical map was published in a special genome issue of
Science that dealt with Arabidopsis (D.W. Meinke, J.M. Cherry, C. Dean, S.D. Rounsley, M. Koornneef [1998] Science 282: 662-682). We present here a comprehensive list and sequence-based map
of 620 cloned genes with mutant phenotypes. This map documents for the
first time the exact locations of large numbers of Arabidopsis genes
that give a phenotype when disrupted by mutation. Such a community-based physical map should have broad applications in Arabidopsis research and should serve as a replacement for the classical genetic map in the future. Assembling a comprehensive list of
genes with a loss-of-function phenotype will also focus attention on
essential genes that are not functionally redundant and ultimately
contribute to the identification of the minimal gene set required to
make a flowering plant.
1
This research was supported by the National
Science Foundation Developmental Mechanisms and Arabidopsis 2010 Programs.
2
Present address: Macalester College, St. Paul, MN 55105.
3
Present address: Swarthmore College, Swarthmore, PA 19081.
4
Present address: College of Osteopathic Medicine,
Kirksville, MO 63501.
*
Corresponding author; e-mail Meinke{at}okstate.edu; fax
405-744-7074.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
© 2003 American Society of Plant Biologists
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