First published online January 23, 2003; 10.1104/pp.102.011007
Plant Physiol, February 2003, Vol. 131, pp. 454-462
Osmotic Stress Tolerance of Transgenic Tobacco Expressing a Gene
Encoding a Membrane-Located Receptor-Like Protein from Tobacco
Plants1
Takashi
Tamura,
Kojiro
Hara,2
Yube
Yamaguchi,
Nozomu
Koizumi, and
Hiroshi
Sano*
Research and Education Center for Genetic Information, Nara
Institute of Science and Technology, Nara 630-0192, Japan
Tobacco (Nicotiana tabacum) genes regulated
during the early stage of responses to wounding were screened by a
modified fluorescence differential display method. Among 28 genes initially identified, a particular clone designated
NtC7 was subjected to further analysis. Its transcripts
were found to accumulate rapidly and transiently within 1 h upon
treatments with not only wounding but also salt and osmotic stresses.
However, jasmonic and abscisic acids and ethylene did not effectively
induce NtC7 transcripts. Amino acid sequence analysis
suggested NtC7 to be a new type of transmembrane protein that belongs
to the receptor-like protein family, and a membrane location was
confirmed in onion (Allium cepa) epidermis cells
transiently expressing an NtC7-green fluorescent protein fusion
protein. Seeds of transgenic tobacco overexpressing NtC7 normally germinated and grew in the presence of 500 mM
mannitol, but not in the presence of 220 mM sodium chloride
or 60 mM lithium chloride. Cuttings of mature transgenic
leaf exhibited a marked tolerance upon treatment with 500 mM mannitol for 12 h, at which concentration wild-type
counterparts were seriously damaged. These results suggested that NtC7
predominantly functions in maintenance of osmotic adjustment
independently of ion homeostasis.
1
This work was supported by the Japan Society for
the Promotion of Science (Research for the Future Program grant no.
JSPS-RFTF 00L01604 and Research Fellowship for Young Scientist no.
06586 to T.T.).
2
Present address: Akita Prefectural University, Faculty
of Bioresource Sciences, Akita 010-0195, Japan.
*
Corresponding author; e-mail sano{at}gtc.aist-nara.ac.jp; fax
81-743-72-5659.
© 2003 American Society of Plant Biologists
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