First published online February 27, 2003; 10.1104/pp.102.011122
Plant Physiol, March 2003, Vol. 131, pp. 1091-1103
The Medicago Species A2-Type Cyclin Is Auxin
Regulated and Involved in Meristem Formation But Dispensable for
Endoreduplication-Associated Developmental
Programs1
François
Roudier,2
Elena
Fedorova,3
Manuel
Lebris,4
Phillippe
Lecomte,
Janos
Györgyey,5
Daniele
Vaubert,
Gabor
Horvath,
Pierre
Abad,
Adam
Kondorosi, and
Eva
Kondorosi*
Institut des Sciences du Végétal, Centre National de la
Recherche Scientifique Unité Propre de Recherche 2355, Avenue de
la Terrasse, 91198 Gif-sur-Yvette, France (F.R., E.F., J.G., D.V.,
G.H., A.K., E.K.); and Unité Interactions Plantes Microorganismes
et Santé Végétale, Institut National de la Recherche
Agronomique, Boite Postale 2078, 06606 Antibes cedex, France (M.L.,
P.L., P.A.)
Phytohormones as well as temporal and spatial
regulation of the cell cycle play a key role in plant development.
Here, we investigated the function and regulation of an alfalfa
(Medicago sativa) A2-type cyclin in three distinct root
developmental programs: in primary and secondary root development,
nodule development, and nematode-elicited gall formation. Using
transgenic plants carrying the
Medsa;cycA2;2
promoter- -glucuronidase gene fusion, in combination with other
techniques, cycA2;2 expression was
localized in meristems and proliferating cells in the lateral root and
nodule primordia. Rapid induction of
cycA2;2 by Nod factors demonstrated that
this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly,
cycA2;2 was repressed in the
endoreduplicating, division-arrested cells both during nodule
development and formation of giant cells in nematode-induced galls,
indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of
cycA2;2 in transgenic plants corresponded
to wild type protein levels and had no apparent phenotype. In contrast,
antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the
formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as
expected from the presence of auxin response elements in the promoter.
Moreover, auxin also affected the spatial expression pattern of this
cyclin by shifting the cycA2;2 expression
from the phloem to the xylem poles.
1
This work was supported by the
Ministère de la Recherche et Technologie (to F.R.), by the
Ministère des Affaires étrangères (to E.F.), and by
the European Commission, European Cell Cycle Consortium Network
Program (grant no. QLG2-99-00454 to G.H.).
2
Present address: Duke University, Department of Biology,
Developmental, Cell and Molecular Biology Group, 104 Research Drive, Durham, NC, 27708.
3
Timiriazev Institute of Plant Physiology, Russian
Academy of Science, Botanicheskaya 35, Moscow 127276, Russia.
4
Laboratoire de Morphogénèse
Végétale, Centre National de la Recherche Scientifique
Unité Mixte de Recherche 6116, Université d'Aix-Marseille
III, Avenue de l'Escadrille Normandie-Niemen, 13397 Marseille cedex
20, France.
5
Biological Research Center of the Hungarian Academy of
Sciences, 6726 Szeged, Temesvari krt. 62, Hungary.
*
Corresponding author; e-mail Eva.Kondorosi{at}isv.cnrs-gif.fr;
fax 33-1-69-82-36-95.
© 2003 American Society of Plant Biologists
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