First published online March 20, 2003; 10.1104/pp.102.017434
Plant Physiol, April 2003, Vol. 131, pp. 1671-1680
Analysis of the Competence to Respond to KNOTTED1 Activity in
Arabidopsis Leaves Using a Steroid Induction
System1
Angela
Hay,
David
Jackson,2
Naomi
Ori,3 and
Sarah
Hake4*
Plant and Microbial Biology Department, University of California,
Berkeley, California 94720
Expression of KNOX
(KNOTTED1-like homeobox) genes in the shoot apical
meristem of Arabidopsis is required for maintenance of a functional
meristem, whereas exclusion of KNOX gene expression from
leaf primordia is required for the elaboration of normal leaf
morphology. We have constructed a steroid-inducible system to regulate
both the amount and timing of KN1
(KNOTTED1) misexpression in Arabidopsis leaves. We
demonstrate that lobed leaf morphology is produced in a dose-dependent
manner, indicating that the amount of KN1 quantitatively affects the
severity of lobing. The KN1-glucocorticoid receptor fusion protein is
not detected in leaves in the absence of steroid induction, suggesting
that it is only stable when associated with steroid in an active state.
By using a second inducible fusion protein to mark exposure of leaf
primordia to the steroid, we determined the stage of leaf development
that produces lobed leaves in response to KN1. Primordia as old as
plastochron 7 and as young as plastochron 2 were competent to respond
to KN1.
1
This work was supported by the National Science
Foundation (grant no. 1BN-0131431).
2
Present address: Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724.
3
Present address: Department of Field Crops and Genetics,
Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, Israel.
4
Present address: Plant Gene Expression Center, U.S.
Department of Agriculture-Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710.
*
Corresponding author; e-mail maizesh{at}nature.berkeley.edu;
fax 510-559-5678.
© 2003 American Society of Plant Biologists
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