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DEVELOPMENT AND HORMONE ACTION

Activation Tagging of a Dominant Gibberellin Catabolism Gene (GA 2-oxidase) from Poplar That Regulates Tree Stature¹

Department of Forest Science, Oregon State University, Corvallis, Oregon 97331–5752 (V.B.B., R.M., C.M., S.H.S.); and University of Lethbridge, Department of Biological Sciences, Lethbridge, Alberta, Canada T1K 3M4 (D.W.P., S.B.R.)

We identified a dwarf transgenic hybrid poplar (Populus tremula x Populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. The cause of the phenotype was a hyperactivated gene encoding GA 2-oxidase (GA2ox), the major gibberellin (GA) catabolic enzyme in plants. The mutation resulted from insertion of a strong transcriptional enhancer near the transcription start site. Overexpression of the poplar GA2ox gene (PtaGA2ox1) caused hyperaccumulation of mRNA transcripts, quantitative shifts in the spectrum of GAs, and similarity in phenotype to transgenic poplars that overexpress a bean (Phaseolus coccineus) GA2ox gene. The poplar PtaGA2ox1 sequence was most closely related to PsGA2ox2 from pea (Pisum sativum) and two poorly known GA2oxs from Arabidopsis (AtGA2ox4 and AtGA2ox5). The dwarf phenotype was reversible through gibberellic acid application to the shoot apex. Transgenic approaches to producing semidwarf trees for use in arboriculture, horticulture, and forestry could have significant economic and environmental benefits, including altered fiber and fruit production, greater ease of management, and reduced risk of spread in wild populations.

¹ This work was sponsored in part by the Tree Genetic Engineering Research Cooperative, by the National Science Foundation Industry/University Cooperative Research Centers Program (grant no. 9980423–EEC), by the Consortium for Plant Biotechnology Research, Inc. (Department of Energy Prime Agreement no. DEFG36–02GO12026), by the U.S. Department of Energy's Biomass Program (contract no. 4000014546 with Oak Ridge National Laboratory; Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract no. DE–AC05–00OR22725), and by the Natural Sciences and Engineering Research Council of Canada (Discovery Grant to S.B.R).

^* Corresponding author; e-mail Steve.Strauss{at}orst.edu; fax 541–737–3093.

Received January 10, 2003; returned for revision February 21, 2003; accepted March 20, 2003.

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