First published online June 5, 2003; 10.1104/pp.102.019273
Plant Physiology 132:1415-1423 (2003)
© 2003 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Integration of Wounding and Osmotic Stress Signals Determines the Expression of the AtMYB102 Transcription Factor Gene1
Marten Denekamp and
Sjef C. Smeekens*
Department of Molecular Plant Physiology, Utrecht University, Padualaan
8, 3584CH Utrecht, The Netherlands
Transcript levels of the Arabidopsis R2R3-AtMYB102 transcription
factor gene, previously named AtM4, are rapidly induced by osmotic
stress or abscisic acid (ABA) treatment. Reporter gene expression studies
revealed that in addition, wounding is required for full induction of the
gene. Histochemical analysis showed a local  -glucuronidase induction
around the wounding site, especially in veins. In ABA-treated plants,
wounding-induced -glucuronidase activity could be mimicked by the wound
signaling compound methyl jasmonate. In silico studies of the
AtMYB102 promoter sequence and its close homolog AtMYB74
demonstrated several conserved putative stress regulatory elements such as an
ABA-responsive element, its coupling element 1 (CE1), and a W box.
Interestingly, further studies showed that the 5'-untranslated region is
essential for the osmotic stress and wounding induced expression of the
AtMYB102 gene. This 5'-untranslated region contains putative
conserved regulatory elements such as a second W box and an overlapping
MYB-binding element. These studies suggest that AtMYB102 expression
depends on and integrates signals derived from both wounding and osmotic
stress.
Article, publication date, and citation information can be found at
www.plantphysiol.org/cgi/doi/10.1104/pp.102.019273.
1 This work was supported by the European Commission (REGIA EU contract no.
QLG2–1999–00876).
*
Corresponding author; e-mail
J.C.M.Smeekens{at}bio.uu.nl;
fax 31–0–302513655.
Received December 23, 2002;
returned for revision February 12, 2003;
accepted April 18, 2003.
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