This Article Full Text Full Text (PDF) All Versions of this Article: 133/3/1091    most recent pp.103.026997v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Tzeng, T.-Y. Articles by Yang, C.-H. Search for Related Content PubMed PubMed Citation Articles by Tzeng, T.-Y. Articles by Yang, C.-H. Agricola Articles by Tzeng, T.-Y. Articles by Yang, C.-H.

DEVELOPMENT AND HORMONE ACTION

Two Lily SEPALLATA-Like Genes Cause Different Effects on Floral Formation and Floral Transition in Arabidopsis¹

Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan 40227 Republic of China

Two AGL2-like MADS-box genes, Lily MADS Box Gene (LMADS) 3 and LMADS4, with extensive homology of LMADS3 to the Arabidopsis SEPALLATA3 were characterized from the lily (Lilium longiflorum). Both LMADS3 and LMADS4 mRNA were detected in the inflorescence meristem, in floral buds of different developmental stages, and in all four whorls of the flower organ. LMADS4 mRNA is also expressed in vegetative leaf and in the inflorescence stem where LMADS3 expression is absent. Transgenic Arabidopsis, which ectopically expresses LMADS3, showed novel phenotypes by significantly reducing plant size, flowering extremely early, and loss of floral determinacy. By contrast, 35S::LMADS4 transgenic plants were morphologically indistinguishable from wild-type plants. The early-flowering phenotype in 35S::LMADS3 transgenic Arabidopsis plants was correlated with the up-regulation of flowering time genes FT, SUPPRESSOR OF OVEREXPRESSION OF CO 1, LUMINIDEPENDENS, and flower meristem identity genes LEAFY and APETALA1. This result was further supported by the ability of 35S::LMADS3 to rescue the late-flowering phenotype in gigantea-1 (gi-1), constans-3 (co-3), and luminidependens-1 but not for ft-1 or fwa-1 mutants. The activation of these flowering time genes is, however, indirect because their expression was unaffected in plants transformed with LMADS3 fused with rat glucocorticoid receptor in the presence of both dexamethasone and cycloheximide.

¹ This work was supported by the Council of Agriculture and National Science Council, Taiwan, Republic of China (grant nos. 90AS–2.1.1–FD–Z1 and NSC90–2317–B–005–002 to C.-H.Y.).

² These authors contributed equally to this paper.

^* Corresponding author; e-mail chyang{at}dragon.nchu.edu.tw; fax 886–4–2285–3126 or 886–4–2285–3527.

Received May 18, 2003; returned for revision June 3, 2003; accepted June 25, 2003.

This article has been cited by other articles:

				Y.-Y. Chang, Y.-F. Chiu, J.-W. Wu, and C.-H. Yang Four Orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS Box Genes Show Novel Expression Patterns and Cause Different Effects on Floral Transition and Formation in Arabidopsis thaliana Plant Cell Physiol., August 1, 2009; 50(8): 1425 - 1438. [Abstract] [Full Text] [PDF]

				M.-K. Chen, I-C. Lin, and C.-H. Yang Functional Analysis of Three Lily (Lilium longiflorum) APETALA1-like MADS Box Genes in Regulating Floral Transition and Formation Plant Cell Physiol., May 1, 2008; 49(5): 704 - 717. [Abstract] [Full Text] [PDF]

				N. Shitsukawa, C. Tahira, K.-i. Kassai, C. Hirabayashi, T. Shimizu, S. Takumi, K. Mochida, K. Kawaura, Y. Ogihara, and K. Murai Genetic and Epigenetic Alteration among Three Homoeologous Genes of a Class E MADS Box Gene in Hexaploid Wheat PLANT CELL, June 1, 2007; 19(6): 1723 - 1737. [Abstract] [Full Text] [PDF]

				S. T. Malcomber and E. A. Kellogg Heterogeneous Expression Patterns and Separate Roles of the SEPALLATA Gene LEAFY HULL STERILE1 in Grasses PLANT CELL, July 1, 2004; 16(7): 1692 - 1706. [Abstract] [Full Text] [PDF]