Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online November 20, 2003; 10.1104/pp.103.022202

Plant Physiology 133:2000-2009 (2003)
© 2003 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
133/4/2000    most recent
pp.103.022202v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chaban, C.
Right arrow Articles by Nick, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chaban, C.
Right arrow Articles by Nick, P.
Agricola
Right arrow Articles by Chaban, C.
Right arrow Articles by Nick, P.
CELL BIOLOGY AND SIGNAL TRANSDUCTION

Auxin Responsiveness of a Novel Cytochrome P450 in Rice Coleoptiles1

Christina Chaban*, Frank Waller2, Masaki Furuya and Peter Nick

Institut für Biologie II, Schänzlestrasse 1, D-79104 Freiburg, Germany (C.C., F.W., P.N.); and Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan (M.F.)

An early auxin-induced gene was isolated from rice (Oryza sativa L. subsp. japonica cv Nihonmasari) coleoptiles by a fluorescent-labeled differential display screen. The full-length gene contains conserved domains characteristic for the cytochrome P450 superfamily. This gene, designated as CYP87A3, was weakly expressed in dark-grown coleoptiles but was up-regulated rapidly and transiently when coleoptile segments were incubated in 5 µM indole-3-acetic acid. This induction by auxin could not be suppressed by cycloheximide. Depletion of segments from endogenous auxin reduced the amount of CYP87A3 transcripts. The CYP87A3 transcript level was rapidly, although transiently, up-regulated in response to light as well. The observed pattern of gene regulation might indicate a role in the suppression of auxin-induced coleoptile growth. The role of CYP87A3 is discussed with respect to auxin signaling in the regulation of coleoptile growth.

1 This work has been supported by a fellowship from the Graduiertenkolleg "Molekulare Mechanismen der pflanzlichen Differenzierung" by the Deutsch Forschungsgemeinschaft (to C.C.), by HARL(B2023) and the Program for Promotion of Basic Research Activities for Innovative Biosciences, Japan (to M.F.), and by funds from the Volkswagen Foundation (to F.W. and P.N.).

2 Present address: Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.022202.

* Corresponding author; e-mail christina.chaban{at}uni-freiburg.de; fax 49-221-470-6897.

Received February 19, 2003; returned for revision March 26, 2003; accepted July 26, 2003.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists